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基于超音速膨胀凝结的油田伴生气脱二氧化碳研究

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中国石油大学学报（自然科学版） 2024年第1期48卷 159-165页

作者：范路韩卓蒲晓莉马立辉李炜边江中国石化胜利油田技术检测中心山东东营257000 中国石油江汉机械研究所有限公司湖北武汉430024 中国石油大学(华东)储运与建筑工程学院山东青岛266580

油田伴生气通常含有大量的二氧化碳等酸性气体,为了提升油田伴生气质量,必须对天然气进行脱二氧化碳处理;将超音速旋流分离技术用于伴生气脱除二氧化碳是一条全新路径,但二氧化碳在超音速流动条件下的冷凝特性有待进一步明晰。建立二氧... 详细信息

油田伴生气通常含有大量的二氧化碳等酸性气体,为了提升油田伴生气质量,必须对天然气进行脱二氧化碳处理;将超音速旋流分离技术用于伴生气脱除二氧化碳是一条全新路径,但二氧化碳在超音速流动条件下的冷凝特性有待进一步明晰。建立二氧化碳在喷管中的相变过程数学模型,讨论二氧化碳超音速凝结流动特性,分析入口温度和压力对二氧化碳超音速凝结特性的影响规律。结果表明:二氧化碳在喷管喉部之后的扩张段发生凝结,生成大量的二氧化碳液滴;随着喷管入口温度增加,尽管凝结的二氧化碳液滴数量增加,但喷管出口和中轴线上的液相质量分数减小,凝结位置越靠近喷管出口;随着喷管入口压力增加,混合气体在喷管出口的压力和温度升高、速度减小,二氧化碳在喷管内凝结产生的液滴数量减少,喷管出口和中心轴线上的液相质量分数越大。

关键词：油田伴生气二氧化碳喷管超音速凝结

小分子化合物与铝佐剂联用对铜绿假单胞菌疫苗候选抗原PA0833保护效果的影响及机制初步研究

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中华微生物学和免疫学杂志 2024年第3期44卷 189-197页

作者：孙恬峻张晓丽夏真萍赵卓章金勇王乂中国海洋大学医药学院海洋药物教育部重点实验室青岛266003 陆军军医大学药学与检验医学系微生物与生化药学教研室重庆400038 陆军军医大学药学与检验医学系临床血液学教研室重庆400038

目的评价二乙基二硫代氨基甲酸钠(sodium diethyldithiocarbamate,DTC)、左旋咪唑(levamisole,LMS)、咪喹莫特(imiquimod,Imi)3个小分子化合物对铜绿假单胞菌疫苗候选抗原PA0833免疫原性和保护效果的影响,并进一步对其机制进行初步研究... 详细信息

目的评价二乙基二硫代氨基甲酸钠(sodium diethyldithiocarbamate,DTC)、左旋咪唑(levamisole,LMS)、咪喹莫特(imiquimod,Imi)3个小分子化合物对铜绿假单胞菌疫苗候选抗原PA0833免疫原性和保护效果的影响,并进一步对其机制进行初步研究。方法抗原PA0833分别与上述3种小分子以及铝佐剂配伍制备疫苗,通过肌肉注射的方式于0、14、21 d分别免疫BALB/c小鼠。收集3次免疫后血清,ELISA测定PA0833特异性IgG抗体水平。采用铜绿假单胞菌PAO1感染免疫后的小鼠,通过生存率、体重变化、临床评分、炎症因子检测和肺组织病理学改变等评价疫苗的保护效果。在此基础上,对小鼠连续7 d肌肉注射DTC,取肺脏组织进行转录组测序分析,并通过流式细胞术检测其对抗原提呈细胞活化的影响。结果联用小分子免疫组能够有效提高PA0833特异性IgG抗体效价,降低细菌感染后肺组织中细菌负荷和TNF-α、IL-6、IL-1β炎症因子水平,显著提高细菌感染后的生存率,其中DTC效果最好。转录组测序结果表明,相比于PBS对照组,DTC处理组样本有121个基因表达上调、18个基因下调;差异表达基因主要富集在免疫信号通路,IL-17信号通路活化明显。流式细胞术结果表明DTC能促进脾细胞中Th17细胞分化,且能促进抗原提呈细胞活化。结论本研究中的3种小分子都能够有效地增强抗原的免疫原性,其中DTC效果最优,可用于新型疫苗佐剂的研发。

关键词：佐剂二乙基二硫代氨基甲酸钠(DTC) 铜绿假单胞菌疫苗

基于形气神三位一体观论糖尿病视网膜病变的发生

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河南中医 2024年第3期44卷 337-342页

作者：王秋玲李吉武林春婷卓小莉广西中医药大学广西南宁530001 广西中医药大学第一附属医院广西南宁530023

人为一整体,形、气、神相互关联,三位一体。形为载体,为气神之宅;气属动力,乃形神之充;神乃主宰,为形气之主。一者太过或不及,余二者必不善。先天禀赋不足,五脏阴虚燥热,消渴乃成,日久视衣为病。三焦通行元气,热酒熏蒸三焦,火郁内燔,精... 详细信息

人为一整体,形、气、神相互关联,三位一体。形为载体,为气神之宅;气属动力,乃形神之充;神乃主宰,为形气之主。一者太过或不及,余二者必不善。先天禀赋不足,五脏阴虚燥热,消渴乃成,日久视衣为病。三焦通行元气,热酒熏蒸三焦,火郁内燔,精血津涸,五脏阴虚燥热,乃致消渴。火热灼损视衣脉络,血溢络外、入于神膏,或血停成瘀,则神光失用。三焦为水谷之道路,三焦不利,水谷精微不布视衣则目昏;水湿不下,泛于视衣则视衣水肿,湿聚成痰则视衣翳障,蒙蔽瞳神。元气不足,升降出入乏力,清阳不上则视衣失荣,浊阴不下则邪阻视衣,视衣水肿或翳障,神光不发。元阳不足,痰湿内阻,则视衣翳障;元阴不足,精血不足,则视衣失荣。消渴阴虚燥热,肺气阴伤损,魄化生乏源,精血失于充养,视衣失濡,则目暗。消渴日久,肝肾亏虚,魂虚及子,魂、神俱虚,魂弱不张,神虚不扬,魂神失用,则不可精明万物。七情失调则乱气机、伤五脏,致视衣为病。形气神三者生理病理一脉相通,形致本病者涵及气神失调,气致本病者兼形神失用,神致本病者不离形气失常,脏腑、三焦、元宗二气、元神、魂魄及七情等一者失常,必牵及余者,遂生本病。临床辨治当同审形气神,注重三者关联。

关键词：糖尿病视网膜病变消渴内障形气神形气神三位一体观

Neoantigen vaccine and neoantigen-specific cell adoptive transfer therapy in solid tumors:Challenges and future directions

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Cancer Innovation 2022年第2期1卷 168-182页

作者： Yanwei Shen Lu Yu xiaoli Xu Shaojun Yu zhuo Yu Shanghai Jianshan Medical Tech Co Ltd ShanghaiChina Department of Surgery The Second Affiliated HospitalZhejiang University School of MedicineHangzhouChina Department of Medical Oncology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijingChina

The phenomenon of tumor hierarchy and genetic instability can be explained by the“two-hits theory”and results in the occurrence of many somatic mutations.The expression of nonsynonymous mutations results in the production of mutant proteins from tumor cells,namely tumor-specific antigens called neoantigens.Because neoantigens do not exist in healthy cells,they have the potential to stimulate antitumor immune responses by CD4+and CD8+T-cell activation without jeopardizing normal tissues.Immunotherapy has reshaped the cancer treatment paradigm in recent decades with the introduction of immune-checkpoint blockade therapy and transgenic Tcell receptor/chimeric antigen receptor T cells.However,these strategies performed poorly in solid tumors because of the obstacles of the immunosuppressive microenvironment caused by regulatory T cells and other suppressor cells.Therefore,other immunotherapeutic strategies are under development,such as personalized vaccines,to trigger de novo T-cell responses against neoantigens and lead to the amplification of tumorspecific T-cell subclones.Neoantigen epitope prediction algorithms have enabled the detection of neoantigens and the creation of tailored neoantigen vaccines as a result of the fast development of next-generation sequencing and cancer bioinformatics.Here we provide an overview of the current neoantigen cancer vaccines and adoptive T-cell transfer therapy with neoantigen-specific lymphocytes.We also discuss the challenges in developing neoantigentargeted immunotherapeutic strategies for cancer.

关键词： neoantigen cancer vaccine immunotherapy tumor microenvironment heterogeneity precision medicine

CsIPT1b and CsUGT85A2 delay female corolla opening in cucumber by regulating the content of cytokinins

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Horticultural Plant Journal 2023年第4期9卷 754-762页

作者： xiaoli Li Haonan Cui Xiaofei Song Chengzhen Sun zhuo Ding Xueyun Zhu Xiaofeng Liu Liying Yan Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization College of Horticulture Science and TechnologyHebei Normal University of Science and TechnologyQinhuangdaoHebei 066004China College of Horticulture Qingdao Agricultural UniversityQingdaoShandong 266109China Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province QingdaoShandong 266109China

Corolla opening is essential for the propagation of unisexual flowering plants,meanwhile commodity fruits with opening corolla have higher commodity value in cucumber fresh food market.Until now,there is little research on the mechanism of corolla opening in cucumber.In a previous paper,we identified a unique cucumber(Cucumis sativus)line(‘6457’)that possesses super ovaries and shows delayed corolla opening when nutrient supplies are abundant.We also previously showed that the expression of CsIPT1b(isopentenyl transferase)and CsUGT85A2(cytokinin glycosyltransferase)is correlated with the delayed opening of the female corolla.Here,we investigated the mechanism of delayed female corolla opening in cucumber by conducting transgenic experiments,hormone assays,and yeast one-hybrid assays.CsIPT1b and CsUGT85A2 positively and negatively regulated delayed female corolla opening,respectively.In CsIPT1b-overexpressing plants and CsUGT85A2 knockout plants,the content of the trans-zeatin riboside,trans-zeatin,and N6-isopentenyladenosine was increased.Overall,our findings indicate that CsIPT1b and CsUGT85A2 were important regulators of the timing of female corolla opening.These genes could thus be targets to cucumber molecular breeding of fruit with flowers remaining on the tip.

关键词： Cucumis sativus CsIPT1b CsUGT85A2 Corolla opening

非小细胞肺癌驱动基因突变的中医辨证分型研究进展

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辽宁中医杂志 2024年第5期51卷 201-205页

作者：蒋凤仙禚孝丽赖靖江王景亮刘寨东付国斌山东第一医科大学附属省立医院肿瘤中心山东济南250021 山东中医药大学第二临床医学院山东济南250002 山东第一医科大学(山东省医学科学院)临床医学院山东济南250117 山东中医药大学附属医院肿瘤科山东济南250014 山东大学附属省立医院肿瘤中心山东济南250021

随着基因组学的不断发展,研究者们对非小细胞肺癌的致病机制有了更深层次的认识。相应地,非小细胞肺癌驱动基因与中医证型之间是否存在相关性亦引起了中西医学者密切关注。通过检索万方数据、中国知网、重庆维普、考克兰图书馆(Cochranc... 详细信息

随着基因组学的不断发展,研究者们对非小细胞肺癌的致病机制有了更深层次的认识。相应地,非小细胞肺癌驱动基因与中医证型之间是否存在相关性亦引起了中西医学者密切关注。通过检索万方数据、中国知网、重庆维普、考克兰图书馆(Cochrance Library)、美国生物技术信息中心下属的医学图书检索系统(PubMed)、荷兰医学文摘数据库(Embase)6个数据库尚未发现有非小细胞肺癌驱动基因与中医证型相关性研究进展综述,故该文系统梳理了肺癌主要驱动基因突变的中医证型研究,发现EGFR基因突变的肺癌患者中医证型主要以气阴两虚证和脾虚痰湿证最为常见;EML4-ALK融合基因、KRAS基因突变的肺癌患者中医证型研究较少且未达成共识;对于突变率小于5%的驱动基因BRAF、ROS1、HER2、RET、NTRK、MET阳性肺癌患者的中医证型尚未有研究报道。同时,期望该文能为非小细胞肺癌驱动基因突变的中医辨证分型研究提供线索和在精准靶向时代拓展中西医结合诊断治疗新思路。

关键词：非小细胞肺癌驱动基因中医证型

内部沸腾法提取紫花地丁抗肿瘤成分总黄酮的工艺优化研究

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广东化工 2024年第2期51卷 42-44,22页

作者：段建荣李春梅高晓莉胡雪娇陈卓沙岭子医院药剂科河北张家口075000

目的:优化紫花地丁总黄酮的内部沸腾法提取工艺,提高总黄酮提取率。方法:考察提取过程中涉及到的7个因素对提取率的影响,即解吸附溶剂浓度、解吸附溶剂用量、解吸附所用时间、提取溶剂浓度、提取温度、提取时间和提取溶剂用量,并在单因... 详细信息

目的:优化紫花地丁总黄酮的内部沸腾法提取工艺,提高总黄酮提取率。方法:考察提取过程中涉及到的7个因素对提取率的影响,即解吸附溶剂浓度、解吸附溶剂用量、解吸附所用时间、提取溶剂浓度、提取温度、提取时间和提取溶剂用量,并在单因素试验基础上,采用Box-Benhnken试验和响应面分析法,优化了紫花地丁总黄酮的提取工艺。结果:先用浓度86%的C_(2)H_(5)OH溶液3 mL/g解吸附20 min,然后用浓度22%C_(2)H_(5)OH溶液25 mL/g在86℃提取紫花地丁2次,每次5 min,结果紫花地丁总黄酮提取得率为13.5%。结论:内部沸腾提取法快速、有效且经济,用于紫花地丁中总黄酮的提取效果良好。

关键词：内部沸腾紫花地丁提取总黄酮工艺优化

Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204

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Molecular Plant 2022年第9期15卷 1440-1456页

作者： xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in Universities of Shandong College of AgricultureLudong UniversityYantai 264025China Computer Network Information Center Chinese Academy of SciencesBeijing 100190China Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China College of Advanced Agricultural Sciences University of Chinese Academy of SciencesBeijing 100049China School of Computer Science and Technology University of Chinese Academy of SciencesBeijing 100049China CAS-JIC Centre of Excellence for Plant and Microbial Science Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Yazhou Bay Seed Laboratory of Hainan Province Sanya 572019China

Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait.

关键词： wheat genome sequencing spatiotemporal gene expression expansion and differentiation of gene family nitrogen use efficiency Kenong 9204

Dorsomorphin induces cancer cell apoptosis and sensitizes cancer cells to HSP90 and proteasome inhibitors by reducing nuclear heat shock factor 1 levels

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Cancer Biology & Medicine 2019年第2期16卷 220-233页

作者： Na Li Ting Wang Zongmeng Li xiaoli Ye Bo Deng Shu zhuo Pengle Yao Mengmei Yang Hong Mei Xiaofang Chen Tengfei Zhu Shiting Chen Hui Wang Jiming Wang Yingying Le CAS Key Laboratory of Nutrition Metabolism and Food Safety Shanghai Institute of Nutrition and Health Shanghai/ Institutes for Biological SciencesUniversity of Chinese Academy of Sciences Chinese Academy of Sciences Shanghai 200031 China Key Laboratory of Food Safety Risk Assessment Ministry of Health Beijing 100022 China Cancer and Inflammation Program Center for Cancer Research National Cancer Institute at Frederick Frederick 21702 MD USA

Objective: Heat shock factor 1(HSF1), a transcriptional regulator of heat shock proteins(HSPs), is an attractive therapeutic target for cancer. However, only a few HSF1 inhibitors have been identified so far.Methods: The mRNA and protein levels of HSF1, HSPs, cleaved PARP, and phosphorylated HSF1 were examined by real-time PCR and Western blot. Forced expression, RNA interference, and immunofluorescence assay were used for mechanistic studies.Cell viability and apoptosis were measured by WST-8 assay and flow cytometry, respectively. Xenograft studies were performed in nude mice to evaluate the effect of dorsomorphin and an HSP90 inhibitor on tumor growth.Results: Dorsomorphin suppressed multiple stimuli-induced and constitutive HSPs expression in cancer cells. Mechanistic studies revealed that dorsomorphin reduced heat-induced HSP expression independent of adenosine monophosphate activated protein kinase. Dorsomorphin reduced heat-stimulated HSF1 Ser320 phosphorylation and nuclear translocation, as well as resting nuclear HSF1 levels in cancer cells. Dorsomorphin induced cancer cell apoptosis by inhibiting HSF1 expression. A structure-activity study revealed that the 4-pyridyl at the 3-site of the pyrazolo [1, 5-a]pyrimidine ring is critical for the anti-HSF1 activities of dorsomorphin. Dorsomorphin sensitized cancer cells to HSP90 and proteasome inhibitors and inhibited HSP70 expression induced by these inhibitors in vitro. In tumor-bearing nude mice, dorsomorphin enhanced HSP90 inhibitor-induced cancer cell apoptosis, tumor growth inhibition, and HSP70 expression.Conclusions: Dorsomorphin is an HSF1 inhibitor. It induces cancer cell apoptosis, sensitizes cancer cells to both HSP90 and proteasome inhibitors, and suppresses HSP upregulation by these drugs, which may prevent the development of drug resistance.Hence, dorsomorphin and its derivates may serve as potential precursors for developing drugs against cancer.

关键词： Dorsomorphin heat shock factor 1 heat shock protein apoptosis cancer