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Transplantation of Mesenchymal Stem Cells Attenuates Acute Liver Failure in Mice via an Interleukin-4-dependent Switch to the M2 Macrophage Anti-inflammatory Phenotype

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Journal of Clinical and Translational Hepatology 2022年第4期10卷 669-679页

作者： Jinglin Wang Haoran Ding Jingchao Zhou Senzhe Xia xiaolei shi Haozhen Ren Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine Nanjing University of Chinese MedicineNanjingJiangsuChina Department of Hepatobiliary Surgery Affiliated Drum Tower Hospital of Nanjing Univer-sity Medical SchoolNanjingJiangsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Nanjing Medical UniversityJiangsuChina Department of Hepatobiliary Surgery Nanjing Drum Tower Hospital Clinical College of Jiangsu UniversityNanjingJiangsuChina

Background and Aims:Transplantation of mesenchymal stem cells(MSCs)derived from bone marrow(BM)is an alternative treatment of acute liver failure(ALF)mainly be-cause of the resulting anti-inflammatory activity.It is not known how MSCs regulate local immune responses and liver regeneration.This study explored the effects of MSCs on hepatic macrophages and the Wnt signaling pathway in ALF.Methods:MSCs were isolated from BM aspirates of C57BL/6J mice,and transplanted in mice with ALF induced by D-galactosamine(D-Gal).The proliferation of hepato-cytes was assayed by immunohistochemical(IHC)staining of Ki-67 and proliferating cell nuclear antigen(PCNA).The levels of key proteins in the Wnt signaling pathway were assayed by western blotting and cytokines were determined enzyme-linked immunosorbent assays(ELISAs).A mac-rophage polarization assay characterized the M1/M2 ratio.The potential role of interleukin-4(IL-4)in the biological ac-tivity of MSCs was determined by silencing of IL-4.Results:Transplantation of allogeneic MSCs significantly attenuated D-Gal-induced hepatic inflammation and promoted liver re-generation.MSC transplantation significantly promoted a phenotypic switch from proinflamatory M1 macrophages to anti-inflammatory M2 macrophages,leading to significant Wnt-3a induction and activation of the Wnt signaling path-way in mice with D-Gal-induced ALF.Of the paracrine fac-tors secreted by MSCs(G-CSF,IL-6,IL-1 beta,IL-4,and IL-17A),IL-4 was specifically induced following transplantation in the ALF model mice.The silencing of IL-4 significantly ab-rogated the phenotypic switch to M2 macrophages and the protective effects of MSCs in both the ALF model mice and a co-culture model in an IL-4 dependent manner.Conclu-sions:In vivo and in vitro studies showed that MSCs ame-liorated ALF through an IL-4-dependent macrophage switch toward the M2 anti-inflammatory phenotype.The findings may have clinical implications in that overexpression of IL-4 may enhance the therapeutic effects of allogeneic MSC transplantation in

关键词： Acute liver failure Mesenchymal stem cells Interleukin 4 Macrophage Wnt signaling pathway

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Podocyte injury and death:New insights into lupus nephritis pathogenesis and therapy

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Rheumatology & Autoimmunity 2023年第4期3卷 193-204页

作者： xiaolei shi Zhipeng Wang Ruihan Tang Wei Chen Department of Nephrology The First Affiliated HospitalSun Yat-sen UniversityGuangdongGuangzhouChina Key Laboratory of Nephrology National Health Commission of ChinaGuangdongChina Key Laboratory of Nephrology Guangdong ProvincialGuangzhouChina

Lupus nephritis(LN),an immune complex-mediated glomerulonephritis,is one of the most severe complications of systemic lupus erythematosus.Current therapeutic regimens for LN mainly involve nonspecific immunosuppressants and biologics targeting immune cells,but these treatments are not always effective and some patients are nonresponsive and susceptible to recurrence.Podocytes are essential for maintaining the glomerular filtration barrier.Injury to and loss of podocytes lead to proteinuria,a hallmark of renal involvement and LN.Podocytes are,therefore,emerging as prospective therapeutic targets for LN.There are numerous mechanisms underlying podocyte malfunction in LN,with autophagy,mitochondrial dysregulation,and programmed cell death being the main processes behind podocyte loss.This review summarizes recent advances in understanding podocyte dysfunction in LN pathogenesis and discusses potential therapeutic interventions targeting podocytes in LN.

关键词： cell death injury lupus nephritis podocyte treatment

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车用小型化内燃机余热回收ORC系统试验

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内燃机学报 2024年第2期42卷 185-191页

作者：石晓磊高妍田华王轩中汽数据(天津)有限公司天津300300 中汽研新能源汽车检验中心(天津)有限公司天津300300 天津大学先进内燃动力全国重点实验室天津300350

基于有机朗肯循环(ORC)原理,针对车用内燃机余热回收系统,搭建了集成化试验台架并开展了台架综合性能试验,探究了内燃机余热回收ORC系统小型化优势和上车应用的潜力.结果表明:设计搭建的试验台架主体尺寸为800 mm×750 mm×620 mm,质量... 详细信息

基于有机朗肯循环(ORC)原理,针对车用内燃机余热回收系统,搭建了集成化试验台架并开展了台架综合性能试验,探究了内燃机余热回收ORC系统小型化优势和上车应用的潜力.结果表明:设计搭建的试验台架主体尺寸为800 mm×750 mm×620 mm,质量为200 kg左右,具有一定的小型化优势;ORC系统整体运行具有较好的稳定性和可靠性,在发动机转速为1300 r/min、转矩为600 N·m工况下,工质质量流量为0.1716 kg/s,蒸发压力达到1.26 MPa,膨胀机运行转速调整为1456 r/min时,ORC系统的输出功率达到2.64 kW.

关键词：车用内燃机余热回收有机朗肯循环

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Corrosion behavior of Al_(0.4)CoCu_(0.6)NiSi_(0.2)Ti_(0.25) high-entropy alloy coating via 3D printing laser cladding in a sulphur environment

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Journal of Materials Science & Technology 2021年第1期60卷 197-205页

作者： Hongxia Wan Dongdong Song xiaolei shi Yong Cai Tingting Li Changfeng Chen Beijing Key Laboratory of Failure Corrosion and Protection of Oil/Gas Facility MaterialsDepartment of Materials Science and EngineeringChina University of Petroleum(Beijing)Beijing 102249China Institute of Advanced Wear&Corrosion Resistant and Functional Materials Jinan UniversityGuangzhou 510632China

High-entropy alloys(HEAs) are of great interest in materials science and engineering communities owing to their unique phase structure.HEAs are constructed with five or more principal alloying elements in equimolar or near-equimolar ratios.Therefore,they can derive their performance from multiple principal elements ratherthan a single element.In this work,three-dimensional printing laser cladding was applied to produce an Al_(0.4)CoCu_(0.6)NiSi_(0.2)Ti_(0.25) HEA coating.The experimental results confirmed that the laser cladding could be used to produce a thin coating of 120 μm in thickness.In the high-temperature laser cladding process,some Fe elements diffused from the substrate to the coating,forming a combination of face-centred cubic and body-centred cubic phase structures.The HEA coating metallurgically bonded well with the substrate.Owing to the increased dislocation density and number of grain boundaries,the HEA coating was harder and had a stronger hydrophobicity than X70 steel.The electrochemistry results showed that the HEA coating had better corrosion resistance than X70 steel.Aluminium oxides formed on the surface of the HEA coating had a certain protective effect.However,because of the laser cladding,the HEA coating generated cracks.In future work,the laser cladding technology will be improved and heat treatment will be implemented to prevent formation of cracks.

关键词： High-entropy alloy coating(HEA coating) Laser cladding Corrosion behaviour

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Brilliant blue G attenuates lipopolysaccharide-mediated microglial activation and inflammation

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Neural Regeneration Research 2013年第7期8卷 599-608页

作者： Kui Lu Jue Wang Bin Hu xiaolei shi Junyi Zhou Yamei Tang Ying Peng Department of Neurology Sun Yat-sen Memorial Hospital Sun Yat-sen University Department of Gynaecology and Obstetrics First Affiliated Hospital of Kunming Medical University Department of Neurosurgery Sun Yat-Sen Memorial Hospital Sun Yat-Sen University Department of Biochemistry and Molecular Biology Medical school of Sun Yat-sen University

Previous studies have confirmed that oxidized adenosine triphosphate, a P2X7 receptor antagonist, attenuates lipopolysaccharide-mediated microglial activation and inflammatory expression following neuronal damage in rat brain. NaCI and temperature may affect the potency of oxidized adenosine triphosphate. Brilliant blue G is a derivative of a widely used food additive and has little toxicity. This study explored the effects of brilliant blue G, a selective P2X7 receptor antagonist, on microglial activation and inflammation. Results demonstrated that brilliant blue G inhibited the release of cydooxygenase-2 and interleukin-6 in BV2 cells. Immunofluorescence displayed that brilliant blue G could suppress lipopolysaccharide-induced microglial activation. This study used RNA interference to block P2X7 receptor expression and found that small interfering RNA also suppressed the release of cyclooxygenase-2 and interleukin-6 in BV2 cells. These results suggested that downregulation of the P2X7 receptor by brilliant blue G was involved in the inhibition of microglial activation and inflammation.

关键词： neural regeneration neurodegenerative disease brilliant blue G P2X7 receptor lipopolysaccharide microglia inflammatory cytokines RNA interference cyclooxygenase-2 interleukin-6 grants-supported paper photographs-containing paper neuroregeneration

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Enhanced thermoelectric properties of hydrothermally synthesized n-type Se&Lu-codoped Bi2Te3

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Journal of Advanced Ceramics 2020年第4期9卷 424-431页

作者： xiaolei shi Xin Ai Qihao Zhang Xiaofang Lu shijia Gu Li Su Lianjun Wang Wan Jiang State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai201620China College of Information Science and Technology Donghua UniversityShanghai201620China State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghai200050China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing100049China Institute of Functional Materials Donghua UniversityShanghai201620China Engineering Research Center of Advanced Glasses Manufacturing Technology Ministry of EducationShanghai201620China

N-type Se&Lu-codoped Bi2Te3 nanopowders were prepared by hydrothermal method and sintered by spark plasma sintering technology to form dense samples.By further doping Se element into Lu-doped Bi2Te3 samples,the thickness of the nanosheets has the tendency to become thinner.The electrical conductivity of Lu0.1Bi1.9Te3−xSex material is reduced with the increasing Se content due to the reduced carrier concentration,while the Seeback coefficient values are enhanced.The lattice thermal conductivity of the Lu0.1Bi1.9Te3−xSex is greatly reduced due to the introduced point defects and atomic mass fluctuation.Finally,the Lu0.1Bi1.9Te2.7Se0.3 sample obtained a maximum ZT value of 0.85 at 420 K.This study provides a low-cost and simple low-temperature method to mass production of Se&Lu-codoped Bi2Te3 with high thermoelectric performance for practical applications.

关键词： thermoelectric material n-type bismuth telluride hydrothermal synthesis co-doping

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Improvement of the assimilable organic carbon （AOC） analytical method for reclaimed water

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Frontiers of Environmental Science & Engineering 2013年第4期7卷 483-491页

作者： Xin ZHAO Hongying HU Shuming LIU Feng JIANG xiaolei shi Mingtang LI Xueqiao XU Environmental Simulation and Pollution Control State Key Joint Laboratory School of Environment Tsinghua University Beijing 100084 China State Environmental Protection Key Laboratory of Microorganism Application and Ris~k Control (MARC)~ Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China College of Resources and Environment Jilin Agriculture University Changchun 130118 China

Microbial growth is an issue of concern that may cause hygienic and aesthetic problems during the transportation and usage of reclaimed water. Assimilable organic carbon （AOC） is an important parameter which determines the heterotrophic bacterial growth potential of water. Pseudomonas fluorescens P17 and Spirillum sp. NOX are widely used to measure AOC in drinking water. The AOC values of various reclaimed water samples determined by P 17 and NOX were compared with those determined by the new strains isolated from reclaimed water in this study. It showed that the conventional test strains were not suitable for AOC measurement of reclaimed water in certain cases. In addition to P17 and NOX, Stenotrophomonas sp. Z J2, Pseudomonas saponi- phila G3 and Enterobacter sp. G6, were selected as test strains for AOC measurement of reclaimed water. Key aspects of the bioassay including inoculum cell density, incubation temperature, incubation time and the pH of samples were evaluated for the newly selected test strains. Higher inoculum density （104 CFU.mL-1） and higher incubation temperature （25℃） could reduce the time required for the tests. The AOC results of various collected samples showed the advantages of the method proposed based on those five strains in evaluating the biologic stability of reclaimed water.

关键词： assimilable organic carbon （AOC） bioassay,biological stability reclaimed water test bacterial strains

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Occurrence of estrogenic endocrine disrupting chemicals concern in sewage plant effluent

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Frontiers of Environmental Science & Engineering 2014年第1期8卷 18-26页

作者： Yan SUN Huang HUANG Ying SUN Chao WANG xiaolei shi Hongying HU Takashi KAMEYA Koichi FUJIE State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua University Beijing 100084 China 302 Military Hospital of China Beijing 100039 China Faculty of Science and Technology Technological and Higher Education Institute of Hong Kong Hong Kong China State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC) Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China Faculty of Environment and Information Sciences Yokohama National University Yokohama 240-8501 Japan

The purpose of this study was to give a worldwide overview of the concentrations of typical estrogenic endocrine disrupting chemicals （EDCs） in the effluent of sewage plants and then compare the concentra- tion distribution of the estrogenic EDCs in ten countries based on the survey data of the estrogenic EDCs research. The concentrations of three main categories （totally eight kinds） of estrogenic EDCs including steroidal estrogens （estrone （El）, estradiol （E2）, estriol （E3） and 17a- ethynylestradiol （EE2））, phenolic compounds （nonylphe- nol （NP） and bisphenol A （BPA）） and phthalate esters （dibutyl phthalate （DBP） and dibutyl phthalate （2- ethylhexyl） phthalate （DEHP）） in the effluents of sewage plants reported in major international journals over the past decade were collected. The statistics showed that the concentration distributions of eight kinds of EDCs were in the range of ng·L^-1 to μg·L^-1. The concentrations of steroidal estrogens mainly ranged within 50.00 ng. L-1, and the median concentrations of El, E2, E3 and EE2 were 11.00, 3.68, 4.90 and 1.00 ng·L^-1, respectively. Phenolic compounds and phthalate esters were found at pg. L-1 level （some individual values were at the high level of 40.00 μg·L^-1）. The median concentrations of BPA, NP, DBP and DEHP were 0.06, 0.55, 0.07 and 0.88 μg·L^-1, respectively. The concentrations of phenolic compounds and phthalate esters in the effluents were higher than that of steroids estrogens. The analysis of the concentration in various ten countries showed that steroids estrogens, phenolic compounds and phthalate esters in sewage plant effluents were detected with high concentration in Canada, Spain and China, respectively.

关键词： estrogenic endocrine disrupting chemicals,concentration distribution sewage plant

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Primary metabolite contents are correlated with seed protein and oil traits in near-isogenic lines of soybean

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The Crop Journal 2019年第5期7卷 651-659页

作者： Jie Wang Pengfei Zhou xiaolei shi Na Yang Long Yan Qingsong Zhao Chunyan Yang Yuefeng Guan College of Resources and Environment Fujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina The Key Laboratory of Crop Genetics and Breeding of Hebei Institute of Cereal and Oil CropsHebei Academy of Agricultural and Forestry SciencesShijiazhuang 050035HebeiChina FAFU-UCR Joint Center for Horticultural Plant Biology and Technology Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina

Soybean(Glycine max[L.]Merr.)is an important source of human dietary protein and vegetable oil.A strong negative correlation between protein and oil contents has hindered efforts to improve soybean seed quality.The metabolic and genetic bases of soybean seed composition remain elusive.We evaluated metabolic diversity in a soybean near-isogenic line(NIL)population derived from parents(JD12 and CMSD)with contrasting seed oil contents.Using GC-TOF/MS,we compared seed primary metabolites of high protein/low oil lines,low protein/high oil lines,and their parents.Principal-components analysis showed that metabolic profiles of all progeny lines could be discriminated based on protein and oil contents.Univariate analysis revealed wide variation and transgressive segregation of metabolites in the population.Twenty-eight annotated metabolites,in particular free asparagine,free 3-cyanoalanine,and L-malic acid,were correlated with seed protein content or seed oil content or seed protein and oil content.These results shed light on the metabolic and genetic basis of soybean seed composition.

关键词： Metabolomics Seed composition Protein Oil Near-isogenic population

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The extending length calculation of hydraulic drive non-metallic completion screen pipe running into ultra-short radius horizontal well

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Petroleum Exploration and Development 2022年第4期49卷 942-954页

作者： BI Yansen XIAN Baoan shi xiaolei GAO Deli MOE Key Laboratory of Petroleum Engineering China University of PetroleumBeijing 102249China Institute of Resources&Environment Henan Polytechnic UniversityJiaozuo 454000China

Focusing on the extending length restriction of the completion screen pipe resistance running into ultra-short radius horizontal well,this paper proposed technology of hydraulic drive completion tubular string running into ultra-short radius horizontal well.Innovative hydraulic drive tools and string structure are designed,which are composed of guide tubing,hydraulic drive tubing and non-metallic completion screen pipe from inside to outside.A novel mechanical-hydraulic coupling model is established.Based on the wellbore structure of an ultra-short radius horizontal well for deep coalbed methane,the numerical calculations of force and hydraulic load on tubular strings were accomplished by the mechanical-hydraulic coupling model.The results show that the extending length of completion tubular string with the hydraulic drive is 17 times that of conventional completion technology under the same conditions.The multi-factor orthogonal design is adopted to analyze the numerical calculations,and the results show that the extending length of the completion tubular string is mainly affected by the completion tubular string structure and the friction coefficient between the non-metallic composite continuous screen pipe and the wellbore.Two series of hydraulic drive completion tubular string structures suitable for ultra-short radius horizontal wells under different conditions are optimized,with the extending limits of 381 m and 655 m,respectively.These researches will provide theoretical guidance for design and control of hydraulic drive non-metallic composite continuous completion screen pipe running into ultra-short radius horizontal wells.

关键词： ultra-short radius horizontal well screen pipe completion hydraulic drive technology non-metallic composite continuous screen pipe screen pipe extending length

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