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Orchid conservation in China from 2000 to 2020:Achievements and perspectives

Plant Diversity 2021年第5期43卷 343-349页

作者： Zhihua Zhou Ronghong Shi Yu Zhang xiaoke xing Xiaohua Jin Department of Wildlife Conservation National Forestry and Grassland AdministrationNo.18Hepingli DongjieBeijing100714China Beijing Botanical Garden Wofosi RdXiangshanBeijing100093China Institute of Medicinal Plant Development Chinese Academy of Medical SciencesBeijing100193China Institute of Botany Chinese Academy of Sciences(IBCAS)Nanxincun 20XiangshanBeijing100093PR China

We review achievements in the conservation of orchid diversity in China over the last 21 *** provide updated information on orchid biodiversity and suggestions for orchid conservation in *** outline national policies of biodiversity conservation,especially of orchid conservation,which provide general guidelines for orchid conservation in *** are now approximately 1708 known species of Orchidaceae in 181 genera in China,including five new genera and 365 new species described over the last 21 *** assessment of risk of extinction of all 1502 known native orchid species in China in 2013 indicated that 653 species were identified as threatened,132 species were treated as data-deficient,and four species endemic to China were classified as *** 1100 species(ca.65%)are protected in national nature reserves,and another~66 species in provincial nature *** 800 native orchid species have living collections in major botanical *** pollination biology of 74 native orchid species and the genetic diversity and spatial genetic structure of 29 orchid species have been investigated at a local scale and/or across species *** mycorrhizal fungal community composition has been investigated in many genera,such as Bletilla,Coelogyne,Cymbidium,Cypripedium,and *** 292 species will be included in the list of national key protected wild plants this *** major tasks for near future include in situ conservation and monitoring population dynamics of endangered species.

关键词： Orchid diversity In situ conservation Ex situ conservation Conservation biology List of national key protected wild plants

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Phylogenetic constrains on mycorrhizal specificity in eight Dendrobium(Orchidaceae) species

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Science China(Life Sciences) 2017年第5期60卷 536-544页

作者： xiaoke xing Xueting Ma Jinxin Men Yanhong Chen Shunxing Guo Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of Education Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100193 China

Plant phylogeny constrains orchid mycorrhizal(OrM) fungal community composition in some orchids. Here, we investigated the structures of the OrM fungal communities of eight Dendrobium species in one niche to determine whether similarities in the OrM fungal communities correlated with the phylogeny of the host plants and whether the Dendrobium-OrM fungal interactions are phylogenetically conserved. A phylogeny based on DNA data was constructed for the eight coexisting Dendrobium species,and the OrM fungal communities were characterized by their roots. There were 31 different fungal lineages associated with the eight Dendrobium species. In total, 82.98% of the identified associations belonging to Tulasnellaceae, and a smaller proportion involved members of the unknown Basidiomycota(9.67%). Community analyses revealed that phylogenetically related Dendrobium tended to interact with a similar set of Tulasnellaceae fungi. The interactions between Dendrobium and Tulasnellaceae fungi were significantly influenced by the phylogenetic relationships among the Dendrobium species. Our results provide evidence that the mycorrhizal specificity in the eight coexisting Dendrobium species was phylogenetically conserved.

关键词： orchid mycorrhiza mycorrhizal network fungal community composition phylogenetic conservatism

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Orchid diversity and distribution pattern in karst forests in eastern Yunnan Province,China

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Forest Ecosystems 2023年第3期10卷 348-356页

作者： Qiang Liu Xunfeng Wu Haitao xing Kuanbo Chi Wenhua Wang Liang Song xiaoke xing Innovation Group of Orchid Conservation and Utilization Yunnan Forestry Technological CollegeJindian RoadPanlong DistrictKunming650224China Jiuwuji Agricultural Service Center Luoping655804China CAS Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesKunming650223China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100193China

Global biodiversity loss and mass extinction of species have raised serious global concerns,especially in fragile *** forest,a well-known fragile ecosystem,is served as a natural laboratory for biogeographical,ecological,evolutionary,and taxonomic ***,species richness and distribution patterns in karst forests largely remain *** has been regarded as the“flagship”group of plant *** this study,we use Wanfengshan Nature Reserve in southeast Yunnan,China,as a model to determine the conservation status of orchids in the karst forest by conducting systematic field surveys in the past three years from 2019 to *** total,we identified 78 orchid species in 35 genera in Wanfengshan Nature *** dominant species were Bulbophyllum andersonii,Eria coronaria,Nervilia mackinnonii and Paphiopedilum *** life forms were also observed,including epiphyte(14.29%),facultative epiphyte(18.18%),lithophyte(23.38%),and terrestrial(44.15%).Orchid species richness showed a bimodal pattern varied with altitude,the higher richness was recorded at 1,500-1,600 and 1,200-1,300 m,while the minimum was below 1,100 *** independent hill is rich in orchid species including‘endemic’species.A total of 25(33.8%)orchid species were considered threatened by the Redlist of Chinese Vascular Plants(Qin et al.,2017),including 3 CR species,5 EN species and 17 VU *** call for more research addressing the mechanisms of ecological adaption,mycorrhizal interactions,and pollination of orchids in karst forests,and effective ways for recovery and conservation of orchids.

关键词： Orchid diversity Limestone forest Resource investigation Threatened status Orchid conservation

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Homeostatic regulation of NAD(H)and NADP(H)in cells

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Genes & Diseases 2024年第5期11卷 285-299页

作者： Luojun Chen xiaoke xing Pingfeng Zhang Lulu Chen Huadong Pei Cancer Center Renmin Hospital of Wuhan UniversityWuhanHubei 430062China Department of Oncology Georgetown Lombardi Comprehensive Cancer CenterGeorgetown University Medical CenterWashingtonDC 20057USA

Nicotinamide adenine dinucleotide(NAD^(+))/reduced NAD^(+)(NADH)and nicotinamide adenine dinucleotide phosphate(NADP^(+))/reduced NADP^(+)(NADPH)are essential metabolites involved in multiple metabolic pathways and cellular ***^(+)and NADH redox couple plays a vital role in catabolic redox reactions,while NADPH is crucial for cellular anabolism and antioxidant *** NAD(H)and NADP(H)homeostasis is crucial for normal physiological activity and is tightly regulated through various mechanisms,such as biosynthesis,consumption,recycling,and conversion between NAD(H)and NADP(H).The conversions between NAD(H)and NADP(H)are controlled by NAD kinases(NADKs)and NADP(H)phosphatases[specifically,metazoan SpoT homolog-1(MESH1)and nocturnin(NOCT)].NADKs facilitate the synthesis of NADP^(+)from NAD^(+),while MESH1 and NOCT convert NADP(H)into NAD(H).In this review,we summarize the physiological roles of NAD(H)and NADP(H)and discuss the regulatory mechanisms governing NAD(H)and NADP(H)homeostasis in three key aspects:the transcriptional and posttranslational regulation of NADKs,the role of MESH1 and NOCT in maintaining NAD(H)and NADP(H)homeostasis,and the influence of the circadian clock on NAD(H)and NADP(H)*** conclusion,NADKs,MESH1,and NOCT are integral to various cellular processes,regulating NAD(H)and NADP(H)*** of these enzymes results in various human diseases,such as cancers and metabolic ***,strategies aiming to restore NAD(H)and NADP(H)homeostasis hold promise as novel therapeutic approaches for these diseases.

关键词： MESH1 NAD(H) NADK NADP(H) Nocturnin

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Mycorrhizal fungal community composition in seven orchid species inhabiting Song Mountain, Beijing, China

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Science China(Life Sciences) 2019年第6期62卷 838-847页

作者： Yanhong Chen Yue Gao Linli Song Zeyu Zhao Shunxing Guo xiaoke xing Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of Education Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College

Mycorrhizal fungi play an important role in the germination and growth of orchids essentially influencing their survival,abundance, and spatial distribution. In this study, we investigated the composition of the mycorrhizal fungal community in seven terrestrial orchid species inhabiting Song Mountain, Beijing, China, using Illumina MiSeq high-throughput sequencing. The mycorrhizal communities in the seven orchids were mainly composed of members of the Ceratobasidiaceae, Sebacinales, and Tulasnellaceae, while a number of ectomycorrhizal fungi belonging to the Russulaceae, Tricholomataceae, Thelephoraceae, and Cortinariaceae were occasionally observed. However, the dominant fungal associates and mycorrhizal community differed significantly among the orchid species as well as subhabitats. These findings confirm the previous observation that sympatric orchid species show different preferences for mycorrhizal fungi, which may drive niche partitioning and contribute to their cooccurrence.

关键词： orchid mycorrhiza fungal community terrestrial orchids fungal diversity

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基于Ag@Cu-MOF/GCE传感器检测VC的研究

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中国食品添加剂 2023年第11期34卷 174-179页

作者：邢晓轲邢鹏胡萍许昌职业技术学院园林与食品工程学院许昌461000

合成制备具有多变体结构的银@铜有机骨架纳米复合材料(Silver@copper organic skeleton nanocomposites,Ag@Cu-MOF)并用玻碳电极(Glassy carbon electrode,GCE)构建Ag@Cu-MOF/GCE以对VC进行快速检测分析。通过对Ag@Cu-MOF和磷酸盐缓冲溶液(Phosphate buffer solution,PBS)的优化确定Ag@Cu-MOF/GCE的最佳工作条件。结果显示Ag@Cu-MOF具有良好的导电性和电催化作用,可有效提高Ag@Cu-MOF/GCE的灵敏度。VC浓度与其对应的氧化峰电流在0.5~1500μmol/L呈线性关系,R2=0.9987,检出限(S/N=3)为0.1942μmol/L,明显优于其它传感器,且Ag@Cu-MOF/GCE对常见共存物葡萄糖、蔗糖、果糖、Ca^(2+)、Mg^(2+)、Zn^(2+)、Fe3+以及多巴胺等有很好的抗干扰能力,加标回收率在96.12%~98.25%之间,可信度较高。Ag@Cu-MOF/GCE操作简单、成本低廉、灵敏度高,在果蔬中VC的快速分析方面潜力巨大。

关键词：维生素C 银纳米粒子铜金属有机骨架纳米复合材料传感器

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磺胺二甲嘧啶抑制低C/N废水生物除磷效能的机制探究

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水处理技术 2024年第9期50卷 45-50页

作者：邢晓轲邢鹏谷俊华许昌职业技术学院河南许昌461000

磺胺二甲嘧啶(SMZ)是废水内高频检出的难降解光谱类抗生素,广泛存在的SMZ对污水生物处理过程构成严重威胁。强化生物除磷工艺是污水厂普遍采用的技术,而SMZ对EBPR的影响的报道至今匮乏。本论文在中温和低有机质进水条件下考察了SMZ含量... 详细信息

磺胺二甲嘧啶(SMZ)是废水内高频检出的难降解光谱类抗生素,广泛存在的SMZ对污水生物处理过程构成严重威胁。强化生物除磷工艺是污水厂普遍采用的技术,而SMZ对EBPR的影响的报道至今匮乏。本论文在中温和低有机质进水条件下考察了SMZ含量对EBPR工艺的影响,并探究了SMZ去除效率、污染物及营养盐的周期性变化规律。最后,通过分析内聚物变化特征及微生物代谢规律揭示了高浓度SMZ抑制EBPR的作用机制。SMZ对EBPR的影响具有含量依赖性,低于1.0 mg/L SMZ未对EBPR产生显著影响,而高浓度SMZ抑制了EBPR对污染物和OP的去除。高浓度SMZ抑制了典型周期内COD的消耗、厌氧释磷及好氧吸磷。高浓度SMZ促进了胞外聚合物但降低了胞内聚合物PAH含量但促进了糖原质代谢。研究结果为EBPR处理含SMZ废水提供数据支撑。

关键词：抗生素 EBPR 内聚物厌氧释磷

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基于TiO_(2)-Fe_(2)O_(3)@Ag/GCE传感器检测日落黄的研究

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食品科技 2024年第9期49卷 333-340页

作者：邢晓轲邢鹏谷俊华许昌职业技术学院园林与食品工程学院河南许昌461000

采用化学合成法合成了一种新型的二氧化钛-三氧化二铁@银纳米复合材料(Titanium dioxide-iron oxide@silver nanocomposite material,TiO_(2)-Fe_(2)O_(3)@Ag),并采用玻碳电极(Glassy carbon electrode,GCE)构建一种新型的TiO_(2)-Fe_(2)O_(3)@Ag/GCE传感器对饮料中的日落黄(Sunset yellow,SY)进行定量分析。通过对TiO_(2)-Fe_(2)O_(3)比例、富集时间以及磷酸盐缓冲溶液(Phosphate buffered saline,PBS)p H值等条件的优化,确定了TiO_(2)-Fe_(2)O_(3)@Ag/GCE的最佳工作条件。结果表明:TiO_(2)-Fe_(2)O_(3)@Ag不仅具有良好的导电性而且对SY具有很好的吸附作用,可有效缩短传感器的富集时间,提高传感器的灵敏度。TiO_(2)-Fe_(2)O_(3)@Ag/GCE的最佳工作条件为TiO_(2)和Fe_(2)O_(3)的比例9:1、PBS的pH值5.0、富集时间120 s。在最佳条件下SY氧化峰电流Ip与其浓度分别在1.0×10^(–4)~0.1μmol/L和0.1~15μmol/L范围内呈线性关系,检出限(S/N=3)为0.0867 nmol/L。此外TiO_(2)-Fe_(2)O_(3)@Ag/GCE对SY中常见干扰物有很好的抗干扰能力,对橘汁饮料中SY的检测结果与高效液相色谱法一致,可信度较高,具有较好的实用价值。

关键词：日落黄电化学传感器纳米复合材料检测

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不同酶对藜麦蛋白肽制备的影响及其抗氧化活性研究

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中国食品添加剂 2022年第1期33卷 69-74页

作者：谷俊华邢晓轲许昌职业技术学院园林与食品工程学院许昌461000

本实验以藜麦蛋白为原料,采用碱性蛋白酶、复合蛋白酶、风味蛋白酶、木瓜蛋白酶、中性蛋白酶分别对藜麦蛋白进行水解,制备藜麦蛋白肽。通过单因素实验对五种酶水解制备的藜麦蛋白肽以蛋白水解度、DPPH自由基清除能力、·OH自由基清除能... 详细信息

本实验以藜麦蛋白为原料,采用碱性蛋白酶、复合蛋白酶、风味蛋白酶、木瓜蛋白酶、中性蛋白酶分别对藜麦蛋白进行水解,制备藜麦蛋白肽。通过单因素实验对五种酶水解制备的藜麦蛋白肽以蛋白水解度、DPPH自由基清除能力、·OH自由基清除能力、ABTS+自由基清除能力、·O^(2-)自由基清除能力为测定指标,研究五种蛋白酶对藜麦蛋白的水解能力和抗氧化活性影响,选出效果相对较佳的酶水解工艺。综合各指标的结果表明,碱性蛋白酶和复合蛋白酶水解能力较强,制备的藜麦蛋白肽抗氧化活性较其他两种酶更好。碱性蛋白酶和复合蛋白酶最佳酶解工艺分别为:酶与底物比0.5%(w/w),底物与水为1∶25(w/v),水解温度50℃,水解时间5h,pH10.0;酶与底物比0.5%(w/w),底物与水为1∶25(w/v),水解温度55℃,水解时间5h,pH8.0。本研究为藜麦蛋白的后期的深加工利用提供一定理论依据。

关键词：藜麦蛋白酶蛋白肽水解度抗氧化性

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脉冲电沉积工艺参数对结晶器铜板Ni-Al_2O_3复合镀层硬度的影响

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电镀与环保 2018年第4期38卷 4-7页

作者：邢晓轲张晓伟许昌职业技术学院园林与食品工程学院河南许昌461000 许昌职业技术学院机电与汽车工程学院河南许昌461000

采用脉冲电沉积技术在结晶器铜板表面制备了Ni-Al_2O_3复合镀层,并研究了脉冲电沉积工艺参数对其硬度的影响。结果表明:Ni-Al_2O_3复合镀层的硬度随占空比的增大(20%~100%)先增大后减小,随平均电流密度的增加(2~15A/dm^2)同样是先增大... 详细信息

采用脉冲电沉积技术在结晶器铜板表面制备了Ni-Al_2O_3复合镀层,并研究了脉冲电沉积工艺参数对其硬度的影响。结果表明:Ni-Al_2O_3复合镀层的硬度随占空比的增大(20%~100%)先增大后减小,随平均电流密度的增加(2~15A/dm^2)同样是先增大后减小;在占空比70%、平均电流密度9A/dm^2的条件下,获得纳米微粒的质量分数为3.76%的Ni-Al_2O_3复合镀层,其硬度达到最大值5.5GPa。复合镀层中纳米微粒的质量分数升高,有利于提高复合镀层的硬度。

关键词：硬度 Ni-Al2O3复合镀层结晶器铜板占空比平均电流密度

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