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Contribution to Confirmed &Synthesized on Mycorrhizae of <i>Tuber indicum</i>s.l. with Two Dominated &Subalpine Broadleaf Trees in Southwestern China

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American Journal of Plant Sciences 2014年第21期5卷 3269-3279页

作者： xiaojuan deng Fuqiang Yu Peigui Liu Key Laboratory of Biodiversity and Biogeography Kunming Institute of Botany Chinese Academy of Sciences Kunming China College of Biological Science and Engineering Beifang University of Nationalities Yinchuan China

The ascomata and mycorrhizae of Tuber indicum s.l. were collected under the forest of broad-leaf species Populus yunnanensis and Quercus pannosa in the field respectively. The symbiotic relationships of both trees with T. indicum were examined and affirmed based on morphology and ITS-rDNA sequences. These two mycorrhizal combinations were successfully produced on artificially controlled substrates and cultural condition. This is the first report of a mycorrhizal association and synthesis between Chinese black truffles and poplars. A hyphal net covering the mantle’s surface of the mycorrhizae was detected in both mycorrhizal combinations. The mycorrhizal colonization of P. yunnanensis and Q. pannosa suggests that T. indicum s.l. has a broader host range and that additional corresponding wood species would be used as candidates for the cultivation of T. indicum. The nuclear-ITS sequences of the mycorrhizae included in the phylogeny of the T. indicum complex revealed that the two clades within the complex do not markedly differ with respect to their preferences for host species or geographical origin. Our results help to explain the wide distribution of both clades of the T. indicum complex. It would be more important for truffle conservation and Chinese black truffle plantation development with these two dominated & alpestrine Populus yunnanensis and Quercus pannosa at subalpine limestone areas in China.

关键词： Host Preference Populus yunnanensis Quercus pannosa Mycorrhizae Truffle Conservation & Plantation

Sedimentary and tectonic evolution of the Banquan pull-apart basin and implications for late Cenozoic dextral strike-slip movement of the Tanlu Fault Zone

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Science China Earth Sciences 2023年第4期66卷 797-820页

作者： Peng SHU Xiwei XU Shaoying FENG Baojin LIU Kang LI Paul TAPPONNIER xiaojuan deng Guihua CHEN Nuan XIA Hongtai XU Jingjing QIN Fubing HE Yan MA Rongzhang ZHENG State Key Laboratory of Earthquake Dynamics Institute of GeologyChina Earthquake AdministrationBeijing 100029China Anhui Earthquake Agency Hefei 230031China Mengcheng National Geophysical Observatory Anhui Earthquake AgencyMengcheng 233500China National Institute of Natural Hazard Ministry of Emergency Management of the People’s Republic of ChinaBeijing 100085China Geophysical Exploration Center China Earthquake AdministrationZhengzhou 450002China Seismic Hazard Mitigation Center of Shandong Province(Engineering Seismology Research Center of Shandong Province) Ji’nan 250014China Beijing Institute of Geological Survey Beijing 100195China

The Banquan Basin is a pull-apart basin with the largest scale and the most prominent structure due to dextral slip of the Tanlu Fault Zone(TLFZ) in late Cenozoic. The depositional history of the basin records the start time and evolution of the right-lateral strike-slip movement of the TLFZ. This paper studies the sedimentary and tectonic evolution of the Banquan Basin by seismic reflection exploration, borehole detection and cosmogenic nuclide chronology. We analyze the coupling relationship between the pull-apart basin and the strike-slip fault and discuss the start time and tectonic significance of the right-lateral strikeslip of the TLFZ. Our study indicates that the Banquan Basin has undergone three evolutionary stages: weak rifting during the pre-pull-apart period, strong extension during the syn-pull-apart period and subsidence during the post-pull-apart period. This implies that the TLFZ, which controls the evolution of the basin, experienced an evolutionary process of weak activity,intensified activity and migration of activity toward the central basin. The sedimentary filling of the basin has strong response to the episodic pull-apart and extension of the basin. Lying upon the basement of the basin, a thin layer of Miocene mudstone slowly accumulated due to local rifting before the strong pull-apart event. Along with the dextral slipping and pull-apart process, the basin was filled with alluvial fan facies, fluvial facies and floodplain facies strata from bottom to top. The latest tectonic movement of the TLFZ in the North China Block in late Cenozoic was dominated by episodic dextral strike-slip motion, and this deformation pattern started at 4.01±1.27 Ma. The latest tectonic deformation in North China since late Cenozoic was governed by eastward extrusion and tectonic orogenesis of the eastern margin of the Qinghai-Xizang Plateau since late Miocene. The eastward thrusting of the Liupanshan fault zone and sinistral shearing of the Qinling fault zone led

关键词： Banquan Basin Strike-slip movement Pull-apart basin Tanlu Fault Zone North China Plain Start time

Arabidopsis ABCG14 forms a homodimeric transporter for multiple cytokinins and mediates long-distance transport of isopentenyladeninetype cytokinins

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Plant Communications 2023年第2期4卷 179-192页

作者： Jiangzhe Zhao xiaojuan deng Jiayun Qian Ting Liu Min Ju Juan Li Qin Yang Xiaoxian Zhu Weiqiang Li Chang-Jun Liu Zhigang Jin Kewei Zhang Institute of Plant Stress Adaptation and Genetic Enhancement Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic PlantsCollege of Chemistry and Life SciencesZhejiang Normal UniversityJinhuaZhejiang 321004P.R.China Key Laboratory of Mollisols Agroecology Northeast Institute of Geography and AgroecologyChinese Academy of SciencesNo.4888 Shengbei StreetChangchun 130102China Biology Department Brookhaven National LaboratoryUptonNY 11973USA

Cytokinins(CKs),primarily trans-zeatin(tZ)and isopentenyladenine(iP)types,play critical roles in plant growth,development,and various stress ***-distance transport of tZ-type CKs meidated by Arabidopsis ATP-binding cassette transporter subfamily G14(AtABCG14)has been well studied;however,less is known about the biochemical properties of AtABCG14 and its transporter activity toward iP-type *** we reveal the biochemical properties of AtABCG14 and provide evidence that it is also required for long-distance transport of iP-type ***14 formed homodimers in human(Homo sapiens)HEK293T,tobacco(Nicotiana tabacum),and Arabidopsis *** activity assays of AtABCG14 in Arabidopsis,tobacco,and yeast(Saccharomyces cerevisiae)showed that AtABCG14 may directly transport multiple CKs,including iP-and tZ-type ***14 expression was induced by iP in a tZ-type CKdeficient double mutant(cypDM)of CYP735A1 and *** atabcg14 cypDM triple mutant exhibited stronger CK-deficiency phenotypes than *** profiling,reciprocal grafting,and 2H6-iP isotope tracer experiments showed that root-to-shoot and shoot-to-root long-distance transport of iP-type CKs were suppressed in atabcg14 cypDM and *** results suggest that AtABCG14 participates in three steps of the circular long-distance transport of iP-type CKs:xylem loading in the root for shootward transport,phloem unloading in the shoot for shoot distribution,and phloemunloading in the root for root *** found that AtABCG14 displays transporter activity toward multiple CK species and revealed its versatile roles in circular long-distance transport of iP-type *** findings provide newinsights into the transport mechanisms of CKs and other plant hormones.

关键词： half-size ABC transporter homodimer isopentenyladenine-type cytokinins long-distance transport circular transport

“Second quantitative characterization”and its application in fractured-vuggy carbonate reservoirs

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Petroleum Exploration and Development 2022年第4期49卷 797-809页

作者： LI Yong deng xiaojuan NING Chaozhong WANG Qi CUI Shiti ZHANG Qi WAN Xiaoguo Research Institute of Petroleum Exploration&Development PetroChinaBeijing 100083China Tarim Oilfield Company PetroChinaKorla 841000China

The Ordovician fracture-vug carbonate reservoirs of Tarim Basin,are featured by developed vugs,caves and *** strong heterogeneity results in huge uncertainty when these reservoirs are quantitatively characterized using merely static seismic *** effective quantitative characterization of the reservoirs has been an urgent problem to be *** study creatively proposes the"second quantitative characterization"technique with the combination of dynamic and static data based on the primary static quantitative characterization and fully considering lots of key influence factors when conducting *** this technique,dynamic analysis methods such as well testing,production rate transient analysis,dynamic reserve evaluation and dynamic connectivity evaluation are used to get understandings on this kind of *** understandings are used as statistical parameters to constrain the inversion of seismic wave impedance to improve the relationship between wave impedance and porosity and determine the fracture-vug morphology,calculate dynamic reserves,and then a more accurate fracture-vugmodel can be selected and used to calculate the oil-water contact inversely based on the results of"second quantitative characterization".This method can lower the uncertainties in the primary quantitative characterization of fracture-vug reservoirs,enhance the accuracy of characterization results significantly,and has achieved good application results in the fracture-vug carbonate reservoirs of Tarim Basin.

关键词： Tarim Basin Ordovician carbonate fracture-vug reservoir uncertainty static quantitative characterization dynamic analysis combination of dynamic and static data second quantitative characterization

Disruption of Shootward Transport of Root-synthesized Cytokinins Delays Plant Senescence in Arabidopsis

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Disruption of Shootward Transport of Root-synthesized Cytoki...

芦根多糖诱导自噬和凋亡抑制非小细胞肺癌A549细胞增殖

第三届全国植物开花·衰老与采后生物学大会

作者： Qilu Yu xiaojuan deng Kewei Zhang Institute of Plant Genetics and Developmental Biology College of Chemistry and Life Sciences Zhejiang Normal University

Cytokinin（CK） is one of the most important phytohormones which plays an essential role in several developmental processes including plant senescence. In most plant species such as tobacco and Brassica napus, high concentration of CKs often delays leaf senescence. But we found the deficiency of root-synthesized CKs in the leaves of Arabidopsis led to an evident delayed-senescence phenotype. In this CK-deficient mutant, root-synthesized CKs cannot be efficiently transported into shoot, resulting in a series changes to the plant. Compared to the wild-type, the growth rate of the rosette leaves was slower in this mutant, leading to smaller rosette diameters and leaf areas;the average flower time was significantly retarded for almost 3 days;the numbers of rosette leaves and lateral buds also decreased apperently. Most important of all, the life span of the mutant was greatly extended for about 15%. After 6 days treatment under dark condition, the chlorophyll content and photosynthetic rate of detached leaves from this mutant reduced less than that of wild-type. Thus, we speculate that the disruption of shootward transport of root-synthesized CKs in Arabidopsis may delay plant senescence with an unknown molecular mechanism.

关键词： Arabidopsis root-synthesized cytokinin leaf senescence

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医药导报 2020年第8期39卷 1041-1046页

作者：邓小娟敖素华四川省医学科学院•四川省人民医院门诊部成都610072 西南医科大学附属中医医院肺病科泸州646000

目的探讨芦根多糖抑制非小细胞肺癌生长、增殖的作用及相关分子机制。方法建立A549细胞体外模型,加入芦根多糖干预后,CCK-8法检测细胞活性,流式细胞仪检测A549细胞凋亡情况,免疫印迹法(Western blotting)检测A549细胞的LC3-Ⅱ/Ⅰ、AKT、... 详细信息

目的探讨芦根多糖抑制非小细胞肺癌生长、增殖的作用及相关分子机制。方法建立A549细胞体外模型,加入芦根多糖干预后,CCK-8法检测细胞活性,流式细胞仪检测A549细胞凋亡情况,免疫印迹法(Western blotting)检测A549细胞的LC3-Ⅱ/Ⅰ、AKT、p-AKT、PI3K、p-PI3K蛋白表达;建立体内移植瘤小鼠模型,加入芦根多糖作用,并以顺铂为阳性对照,观察芦根多糖对荷瘤小鼠肿瘤生长影响,免疫组化法检测移植瘤小鼠A549细胞肿瘤组织凋亡蛋白Bax、Bcl-2、Caspase-3蛋白表达。结果100μg·mL^-1的芦根多糖在48及72 h时显著抑制A549细胞增殖(P<0.05);芦根多糖可使A549细胞凋亡的比例显著升高(P<0.05),A549细胞增殖的LC3-Ⅱ/Ⅰ、p-AKT/AKT、p-PI3K/PI3K比值显著升高(P<0.05),而AKT、PI3K蛋白表达水平无明显变化。裸鼠移植A549细胞7 d后,腋下开始生长肿瘤块,第13天时小鼠肿瘤体积达到90 mm 3,模型成功率90.0%;给药第9天开始,顺铂组及芦根多糖组肿瘤生长曲线平缓,肿瘤体积明显低于对照组(P<0.05)。给药第18天时,与对照组比较,顺铂和芦根多糖对小鼠肿瘤的抑制率分别为39.1%,35.9%(P<0.05),瘤细胞的凋亡蛋白Bax、Casepes-3表达显著升高(P<0.05),Bcl-2蛋白表达显著降低(P<0.05)。结论芦根多糖可能通过激活自噬和促进凋亡抑制A549细胞增殖,可作为一种潜在的非小细胞肺癌的治疗药物。

关键词：芦根多糖自噬细胞凋亡非小细胞肺癌 A549细胞株

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聚焦堆栈中空间几何结构的深度估计

光学学报 2023年第20期43卷 103-114页

作者：罗天琦邓小娟刘畅邱钧北京信息科技大学应用数学研究所北京100101

利用聚焦堆栈估计场景深度是计算成像领域中的重要技术手段。提出三维自适应加权全变分计算框架,用于解决场景中弱纹理区域和遮挡区域深度线索丢失导致深度估计不准确的问题。相比传统二维引导滤波方法,所提三维优化框架不仅考虑聚焦堆... 详细信息

利用聚焦堆栈估计场景深度是计算成像领域中的重要技术手段。提出三维自适应加权全变分计算框架,用于解决场景中弱纹理区域和遮挡区域深度线索丢失导致深度估计不准确的问题。相比传统二维引导滤波方法,所提三维优化框架不仅考虑聚焦堆栈和聚焦测度中共同蕴藏的场景几何结构,避免在深度图中错误地引入场景物理信息,还充分考虑聚焦堆栈和聚焦测度沿图像序列方向的结构特点,实现更高程度的数据保真。模拟数据和实际数据实验结果表明,所提方法能够有效提升聚焦堆栈估计深度的精度。

关键词：图像处理聚焦堆栈聚焦测度深度估计结构一致性

“互联网+”赋能农村学校美育教学变革的内在机理与实践路向

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电化教育研究 2023年第3期44卷 71-77页

作者：邓小娟王福平西北师范大学甘肃兰州730070

随着互联网与教育融合不断深入,以“互联网+”赋能农村学校美育教学变革是农村教育发展的方向和趋势,是促进城乡美育教学均衡发展的重要举措。由于当前农村学校美育教学存在美育观念相对落后,师资、教学条件不足,课程开设和评价方式单... 详细信息

随着互联网与教育融合不断深入,以“互联网+”赋能农村学校美育教学变革是农村教育发展的方向和趋势,是促进城乡美育教学均衡发展的重要举措。由于当前农村学校美育教学存在美育观念相对落后,师资、教学条件不足,课程开设和评价方式单一等现实问题亟待解决。研究从互联网空间的特性深入剖析了“互联网+”赋能农村学校美育教学变革的内在机理,认为“互联网+”可以拓展农村学校美育课程教学场域、补充农村学校美育教学资源短板、细化农村学校美育教学评价结果、推动农村学校美育教学群智协同、构建农村学校美育新型师生关系和促进农村学校美育联动有机发展。基于此,研究进一步从夯实基础设施、优化技术手段、落实发展任务、完善课程建设和健全保障体系等方面提出了“互联网+”赋能农村学校美育教学变革的实践路向,以期助力农村学校美育教学工作者正确认识与践行“互联网+”赋能农村学校美育教学。

关键词：互联网+美育农村学校教学变革优质教学资源实践路向

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核磁共振波谱法测定试剂中残留1H含量

分析试验室 2023年第1期42卷 45-48页

作者：邓小娟王意丁国生天津大学分析测试中心天津300072

提出了一种核磁共振氢谱外标法快速测定氘代试剂中残留1H的方法,采用内标法标定氘代氯仿对照品,得到氘代氯仿对照品的氘代率,以此对照品作为外标,测定氘代试剂样品氢谱,通过分析残留1H积分面积比值计算氘代试剂样品的氘代率。结果表明,... 详细信息

提出了一种核磁共振氢谱外标法快速测定氘代试剂中残留1H的方法,采用内标法标定氘代氯仿对照品,得到氘代氯仿对照品的氘代率,以此对照品作为外标,测定氘代试剂样品氢谱,通过分析残留1H积分面积比值计算氘代试剂样品的氘代率。结果表明,氘代氯仿中残留氯仿浓度与其1H信号积分面积线性关系良好,方法可用于实际样品测定。

关键词：核磁共振氘代氯仿氘代率