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Catalytic effects of V-and O-species derived from PrF_(3)/V_(2)C for efficient hydrogen storage in MgH_(2)

Nano Research 2024年第8期17卷 7117-7125页

作者： Zhenluo Yuan Yuhang Wang Xiuxiu Zhang Shuyan Guan xiaojiao Wang Liqiang Ji Qiuming Peng Shumin han Yanping Fan Baozhong Liu College of Chemistry and Chemical Engineering Henan Polytechnic UniversityJiaozuo 454000China Henan Famous Diamond Co. Ltd.Mengzhou 454763China School of Chemical Engineering Zhengzhou UniversityZhengzhou 450001China Collaborative Innovation Center of Steel Technology University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Metastable Materials Science and Technology Yanshan UniversityQinhuangdao 066004China

Magnesium hydride(MgH_(2))is considered as an ideal hydrogen storage material with excellent hydrogen capacity,but the slow kinetics impedes its ***,an efficient additive of V2C MXene-anchored PrF_(3) nanoparticles(PrF_(3)/V_(2)C)was synthesized,which presents excellent catalytic effect in improving the reversibility and stability of hydrogen storage in MgH_(2).The initial dehydrogenation temperature of the 5 wt.% PrF_(3)/V_(2)C-containing MgH_(2)(182℃) is 105℃ lower than that of pure MgH_(2),and 6.5 wt.%hydrogen is rapidly released from 5 wt.%PrF_(3)/V_(2)C-added MgH_(2)sample in 6 min at 240℃.In addition,5 wt.%PrF_(3)/V_(2)C-containing MgH_(2) sample possesses outstanding reversible hydrogen storage capability of 6.5 wt.% after 10 cycles of dehydrogenation and *** analysis shows that the introduction of Pr improves the stability of V-species(V^(0)and V^(2+))and O-species(lattice oxygen(OL)and vacancy oxygen(OV))formed during ball milling,promotes the interaction between V-species and O-species,and enhances their reversibility,which contributes to the significant improvement in re/dehydrogenation reversibility and cycling stability of MgH_(2).This study provides effective ideas and strategies for the purpose of designing and fabricating high-efficient catalysts for solid-state hydrogen storage materials.

关键词： hydrogen storage materials MgH_(2) dehydrogenation reversibility cyclic stability

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Lignin biosynthesis and accumulation in response to abiotic stresses in woody plants

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Forestry Research 2022年第1期2卷 79-88页

作者： xiaojiao han Yanqiu Zhao Yinjie Chen Jing Xu Cheng Jiang Xiaqin Wang Renying Zhuo Meng-Zhu Lu Jin Zhang State Key Laboratory of Tree Genetics and Breeding Key Laboratory of Tree Breeding of Zhejiang ProvinceResearch Institute of Subtropical ForestryChinese Academy of ForestryHangzhouZhejiang 311400China State Key Laboratory of Subtropical Silviculture College of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouZhejiang 311300China

Woody plants have to experience various abiotic stresses due to their immobility and perennial ***,woody plants have evolved a series of specific regulation pathways in physiological and molecular mechanisms to deal with adverse *** with herbaceous plants,perennial woody plants have the advantages of developed roots and hard stems,and increased secondary xylem,which can strengthen the vascular system of the *** lignification process involves the lignin deposition on the cell wall by oxidation and polymerization of lignin monomer,which plays an important role in abiotic stress *** review focuses on recent progress in the biosynthesis,content,and accumulation of lignin in response to various abiotic stresses in *** role of transcription factors is also discussed in regulating lignin biosynthesis to enhance abiotic stress tolerance via changing cell wall *** woody plants shared similar lignin biosynthesis mechanisms with herbaceous plants,the temporal and spatial expression and stress response profiles of lignin biosynthetic genes provide the basis for the differences in stress tolerance of various *** in-depth understanding of the role of lignin in the abiotic stress tolerance of woody plants will lay the foundation for the next step in tree resistance breeding through genetic engineering.

关键词： species stress breeding

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考虑风电爬坡灵活调节的碳捕集电厂低碳经济调度

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电工技术学报 2024年第7期39卷 2033-2045页

作者：韩丽王冲于晓娇喻洪波王晓静中国矿业大学电气工程学院徐州221116

为实现“双碳”目标,风电等可再生能源的使用是减少碳排放的根本途径,而碳捕集电厂是降低碳排放的直接手段。但风电具有与生俱来的波动性,风电的骤升骤降会影响碳捕集电厂“电碳耦合”特性,从而对碳捕集效果带来不利影响。而储液罐通过... 详细信息

为实现“双碳”目标,风电等可再生能源的使用是减少碳排放的根本途径,而碳捕集电厂是降低碳排放的直接手段。但风电具有与生俱来的波动性,风电的骤升骤降会影响碳捕集电厂“电碳耦合”特性,从而对碳捕集效果带来不利影响。而储液罐通过增减自身溶液量可近似地存储释放能量,为解决上述问题提供了可能。该文首先搭建碳捕集电厂数学模型,分析储液罐通过增减溶液量带来的储放能量与碳捕集设备能耗之间的关联,研究储液罐的双向调节能力;然后,提出上爬坡碳超前补偿和下爬坡碳滞后补偿调度策略,双向调节储液罐使碳捕集电厂电碳解耦;接着,构建跟随风电爬坡进行调节的低碳经济调度模型;最后,对含碳捕集电厂、高碳电厂和风电场的电力系统进行仿真,证明所提方法的有效性。

关键词：碳减排风电爬坡碳捕集电厂碳补偿

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The Nramp6 Metal Transporter from Sedum alfredii Contributes to Cadimium Toxicity

The Nramp6 Metal Transporter from Sedum alfredii Contributes...

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全国农业生物化学与分子生物学第十五届学术研讨会

作者： Shuangshuang Chen xiaojiao han Jie Fang Mingying Liu Jian Sang Jing Jiang Renying Zhuo State Key Laboratory of Forest Genetics and Breeding Key Lab of Tree Genomics The Research Institute of Subtropical of ForestryChinese Academy of Forestry Chemical Biology Center Lishui Institute of Agricultural Sciences

Plant natural resistance-associated macrophage protein(Nramp) families play an important role in tolerance of heavy metal *** this study,we cloned and identified the SaNramp6 gene from the heavy metal-hyperaccumulating plant Sedum alfredii and analyzed its function in transgenic Arabidopsis *** 1638-bp cDNA for SaNramp6 encodes 545 amino ***6 was strongly expressed in roots and leaves,and its transcription of root increased in response to Cd stress,with expression reaching a maximum in roots 1 week after ***6 fused to green fluorescent protein was localized to the plasma membrane in both Nicotiana benthamiana lower leaf epidermal cells and onion(Allium cepa) epidermal *** *** expressing SaNramp6 exhibited higher Cd toxicity as compared with the control,with higher cadmium concentration,MDA content,electrolyte leakage and antioxidant enzyme activity,as well as lower chlorophyll content under Cd *** and NBT staining also indicated that the transgenic plants accumulate more reactive oxygen species compared to the control when exposed to ***,these results suggest that overexpression of SaNramp6 plays a significant role in improving the cadmium accumulation,and the gene may be useful for the biotechnological development of transgenic plants to phytoremediate soil contaminated with heavy metals.

关键词： Nramp Sedum alfredii Cadmium stress overexpression

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The research of photosynthesis and chlorophyll fluorescent parameters variation between two species of Sedum under cadmium treatment

The research of photosynthesis and chlorophyll fluorescent p...

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2016年全国植物生物学大会

作者： Liu Zheng Xuelian He Wenmin Qiu xiaojiao han Mingying Liu Zhengquan He Renying Zhuo Biotechnology Research Center China Three Gorges University Research Institute of Subtropical Forestry The Chinese Academy of Forestry

The hyperaccumulating plant is a promising phytoremediation candidate accumulating substantial heavy metal ions without obvious signs of poisoning. We used the hyperaccumulating ecotype of Sedum alfredii hance(HE) and non-hyperaccumulating ecotype of Sedum alfredii hance(NHE) for physiology experiment. Hydroponic experiments were conducted using a modified culture solution. We determined the chlorophyll content,photosynthetic parameter,chlorophyll fluorescence characteristics at different time points. The results showed that along with the prolonging of stress time,the chlorophyll content a(Chla) and the chlorophyll content b(Chlb) finally reached a steady after a big drop. The Pn,Gs,Tr,Ci reduced at the beginning of stress,and then showed a rising trend,which illustrated the cadmium tolerance of two species of Sedum. The difference of Pn and Tr between two species of Sedum show that the cadmium tolerance of HE was stronger than the NHE. Compared these chlorophyll fluorescence characteristics(F0,Fm,ΦPSII、NPQ,q P,ETR),we found that cadmium tolerance of two species of Sedum was completely different. Verifing the cadmium tolerance of two species of Sedum would have a great help for the control of environmental pollution.

关键词： hyperaccumulating ecotype Sedum alfredii non-hyperaccumulating ecotype Sedum alfredii cadmium stresses chlorophyll photosynthetic characters fluorescence parameter

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伴矿景天WRKY基因家族鉴定及镉胁迫响应分析

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南京林业大学学报（自然科学版） 2023年第2期47卷 49-60页

作者：王剑超邱文敏金康鸣陆铸畴韩小娇卓仁英刘晓光何正权三峡区域植物遗传与种质创新重点实验室(三峡大学) 三峡大学生物与制药学院湖北宜昌443002 中国林业科学研究院亚热带林业研究所浙江省林木育种技术研究重点实验室浙江杭州311400 唐山市农业科学研究院河北唐山063001

【目的】WRKY转录因子在植物响应非生物胁迫过程中发挥着重要的调控作用,目前超积累植物伴矿景天(Sedum plumbizincicola)WRKY转录因子的研究较少。进行SpWRKY基因家族的全基因组鉴定及其在镉胁迫下的表达模式分析可为后续SpWRKYs基因... 详细信息

【目的】WRKY转录因子在植物响应非生物胁迫过程中发挥着重要的调控作用,目前超积累植物伴矿景天(Sedum plumbizincicola)WRKY转录因子的研究较少。进行SpWRKY基因家族的全基因组鉴定及其在镉胁迫下的表达模式分析可为后续SpWRKYs基因克隆和耐镉功能分析提供参考。【方法】对伴矿景天WRKY基因家族进行全基因组鉴定和生物信息学分析,利用转录组数据和qRT-PCR技术检测WRKY基因在镉胁迫下的表达模式并进行转基因拟南芥耐镉性的分析。【结果】伴矿景天基因组中共鉴定出77个SpWRKYs,非均匀地分布在各条染色体上;系统进化树分析发现,伴矿景天WRKY成员被分成3大类群(Ⅰ—Ⅲ),第Ⅱ类群又被分成5个亚群(Ⅱa—e);共线性分析发现,伴矿景天WRKY基因家族和拟南芥(Arabidopsis thaliana)之间存在7个共线基因对,和玉吊钟(Kalanchoe fedtschenkoi)之间存在53个共线基因对;SpWRKYs基因之间存在19个片段复制基因对;启动子序列分析发现,SpWRKYs启动子有多种与激素和逆境响应等相关的顺式调控元件;镉胁迫表达分析发现,7个SpWRKYs受镉胁迫显著上调表达,整体表达趋势呈现先增后减的状态;在拟南芥中过表达SpWRKY69发现,SpWRKY69可以增强Cd离子向地上部的转运速率,对植株镉耐性起到负调控作用。【结论】伴矿景天WRKY基因家族与其他物种的WRKY家族结构相似,片段复制是其主要进化动力之一。镉胁迫条件下部分成员相对表达量显著变化,而SpWRKY69能增加Cd离子向地上部的转运速率,故其他WRKY成员可能也参与调控植株镉耐性。

关键词：伴矿景天 WRKY转录因子镉胁迫基因表达拟南芥转基因

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Compatibility of protein extraction methods with proteomic analysis for the Tung trees(Vernicia fordii)seed

Compatibility of protein extraction methods with proteomic a...

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第十三届全国植物基因组学大会

作者： Zhiyong Zhan,Yicun Chen,xiaojiao han,Qinqin Cui,Yangdong Wang~* Research Institute of Subtropical Forestry,Fuyang 311400,Zhejiang,China

An effective protein extraction method is crucial to separate the proteins by twodimensional polyacrylamide gel electrophoresis(2D-PAGE).Tung tree(Vernicia fordii) seed,a recalcitrant plant tissue with large amounts of compounds can interfere with the proteins extraction,even affect the resolution of 2D-PAGE images,was used

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Exploration of the “Four-Three-Four” Internship and Training Mode in Higher Vocational Colleges Under the Modern Vocational Education System

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Journal of Contemporary Educational Research 2024年第3期8卷 111-117页

作者： Jifeng Liu Na han xiaojiao Li Jing Wang Xuezhen Feng Shandong Maritime Vocational College Weifang 261108Shandong ProvinceChina College of Educational Sciences Xinjiang Normal UniversityUrumqi 830017Xinjiang Uygur Autonomous RegionChina Weifang City Government Kindergarten Weifang 261041Shandong ProvinceChina

This article mainly introduces the innovative internship and training model of vocational colleges,including five aspects such as“four-party collaboration,three-stage progression,four-way integration,value-added evaluation,and double selection and promotion.”The model aims to improve students’practical skills and professional quality to better adapt to market demand and social *** article also presents the prospects of the future internship and training model,including strengthening cooperation and communication with industry enterprises,focusing on students’personalized development and practical skill cultivation,and establishing a scientific and objective evaluation and feedback mechanism.

关键词： Four-party collaboration Three-stage progression Four-way integration Value-added evaluation Double selection and promotion

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Selection of reliable reference genes for gene expression studies using real-time PCR in tung tree

Selection of reliable reference genes for gene expression st...

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第十三届全国植物基因组学大会

作者： xiaojiao han,Yicun Chen,Zhiyong Zhan,Qinqin Cui,Yangdong Wang~* Research Institute of Subtropical Foresty,Chinese Academy of Foresty,Fuyang 311400, China

Quantitative real-time PCR(RT-qPCR) has become an accurate and widely used technique to analyze expression levels of selected *** is very necessary to select appropriate reference genes for gene expression *** tree (Vernicia fordii),belongs to a species of the genus Vernicia in the spurge (Euphorbiaceae) *** and identification of stable reference genes will

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Molecular breeding of water lily: engineering cold stress tolerance into tropical water lily

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Horticulture Research 2018年第1期5卷 91-101页

作者： Cuiwei Yu Guirong Qiao Wenmin Qiu Dongbei Yu Shirong Zhou Yan Shen Guanchun Yu Jing Jiang xiaojiao han Mingying Liu Liangsheng Zhang Fei Chen Yuchu Chen Renying Zhuo State Key Laboratory of Tree Genetics and Breeding Chinese Academy of Forestry100091BeijingChina Zhejiang Humanities Landscape Co. Ltd.Hangzhou Tianjing Aquatic Botanical Garden310000HangzhouZhejiangChina Key Laboratory of Tree Breeding of Zhejiang Province The Research Institute of Subtropical forestryChinese Academy of Forestry311400HangzhouZhejiangChina State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsFujian Provincial Key Laboratory of Haixia Applied Plant Systems BiologyFujian Agriculture and Forestry University350002FuzhouChina

Water lilies(order Nymphaeales)are rich in both economic and cultural *** grow into aquatic herbs,and are divided into two ecological types:tropical and *** tropical water lilies have more ornamental and medicinal values compared to the hardy water lily,the study and utilization of tropical water lilies in both landscaping and pharmaceutical use is greatly hindered due to their limited planting *** water lilies cannot survive the winter in areas beyond 24.3°N ***,the transgenic pipeline through the pollen-tube pathway was generated for water lily for the first *** improve cold stress tolerance of tropical water lilies,a gene encoding choline oxidase(CodA)driven by a cold stress-inducible promoter was transformed into a tropical water lily through the pollen-tube *** independent transgenic lines were tested for survival rate during two winter seasons from 2015 to 2017 in hangzhou(30.3°N latitude).PCR and southern blot detection revealed that the CodA gene had been integrated into the *** transcription PCR showed that CodA gene was induced after cold stress treatment,and further quantitative real-time PCR revealed different expressions among six 4 lines and line 3 had the highest *** physiological experiments showed that after cold stress treatment,both the conductivity and malondialdehyde(MDA)levels from transgenic plants were significantly lower than those of non-transgenic plants,whereas the content of betaine and the activity of superoxide dismutase,catalase,and peroxidase were higher than those from non-transgenic *** results suggest that expression of exogenous CodA gene significantly improved the cold stress tolerance of tropical water lilies through a wide range of physiological *** results currently expanded a six-latitude cultivating area of the tropical water *** results not only illuminate the bright future for water lily breeding but will als

关键词： tropical breeding stress

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