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Rational modulation of electronic structure in PtAuCuNi alloys boosts efficient electrocatalytic ethanol oxidation assisted with energy-saving hydrogen evolution

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Journal of Energy Chemistry 2024年第6期93卷 557-567,I0014页

作者： Hu yao Yinan Zheng Xin Yu Songjie Hu Baolian Su xiaohui Guo Key Lab of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education The College of Chemistry and Materials ScienceNorthwest UniversityXi’an 710069ShaanxiChina Department of Inorganic Chemistry University of NamurBruxellesB-5000 NamurBelgium

Compared to conventional electrocatalytic water splitting,electrocatalytic ethanol oxidation reaction(EOR)along with hydrogen production is considered a more energy-efficient ***,we prepared a type of novel quaternary alloy catalyst(PtAuCuNi@NF)that exhibits excellent activity for EOR(0.215 V at 10 mA cm^(-2))and hydrogen evolution reaction(HER)(7 mV at 10 mA cm^(-2)).Experimental results demonstrated that both Cu and Ni modulated the electronic environment around Pt and *** electron-rich active center facilitates the rapid adsorption and dissociation of reactants and intermediates for both EOR and ***,in the ethanol-assisted overall water splitting(E-OWS),a current density of 10 mA cm^(-2)was achieved at 0.28 ***,an advanced acid-base self-powered system(A-Bsps)that can achieve a self-powered voltage of 0.59 V was ***,the self-driven hydrogen production with zero external power supply was realized by integrating A-Bsps with the E-OWS *** interesting results can provide a feasible strategy for designing and developing advanced nanoalloy-based materials for clean energy integration and use in various fields.

关键词： Pt-based alloy Electronic structure Ethanol oxidation Self-powered system Overall water splitting

猪圆环病毒4型Cap蛋白单克隆抗体的制备与鉴定

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畜牧与兽医 2022年第4期54卷 67-71页

作者：姚晓慧连拯民袁丽莉胡丹鹤刘盼娆朱振邦李向东扬州大学兽医学院江苏扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009 扬州大学教育部农业与农产品安全国际合作联合实验室江苏扬州225009

为制备猪圆环病毒4型(PCV4)Cap蛋白单克隆抗体,以原核表达的重组Cap蛋白免疫6~8周龄小鼠,三次免疫后取免疫小鼠脾细胞与SP2/0骨髓瘤细胞进行融合。利用亚克隆技术和间接免疫荧光(IFA)方法筛选到2株阳性单克隆杂交瘤细胞株,分别命名为5H9... 详细信息

为制备猪圆环病毒4型(PCV4)Cap蛋白单克隆抗体,以原核表达的重组Cap蛋白免疫6~8周龄小鼠,三次免疫后取免疫小鼠脾细胞与SP2/0骨髓瘤细胞进行融合。利用亚克隆技术和间接免疫荧光(IFA)方法筛选到2株阳性单克隆杂交瘤细胞株,分别命名为5H9和2F3。间接免疫荧光和Western blot试验表明,2株单抗均能特异性识别293T细胞中特异表达的Cap蛋白,且5H9和2F3的IFA效价分别为1∶12 800和1∶6 400。亚类鉴定结果表明,两株单抗重链均属于IgG1,轻链类型为kappa型。本研究制备的抗PCV4 Cap特异性单抗,为PCV4检测方法的建立和流行病学调查提供了有效的生物材料,也为该病毒的分离鉴定与Cap蛋白功能的探究奠定了基础。

关键词：猪圆环病毒4型衣壳蛋白原核表达单克隆抗体

不同动物粪源葡萄球菌耐药性调查及耐药基因检测分析

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新疆农业科学 2020年第5期57卷 967-973页

作者：杨紫嫣姚晓慧马木尔·阿克木汉王舒丰王凯夏利宁新疆农业大学动物医学学院乌鲁木齐830052

【目的】研究新疆霍城县不同动物粪源葡萄球菌对临床常用抗菌药物的耐药情况。【方法】新疆霍城县主要的规模化养殖场分别采集猪、鸡、牛及羊的粪便。采用琼脂稀释法对分离出的葡萄球菌进行最小抑菌浓度测定。通过PCR方法检测相应耐药... 详细信息

【目的】研究新疆霍城县不同动物粪源葡萄球菌对临床常用抗菌药物的耐药情况。【方法】新疆霍城县主要的规模化养殖场分别采集猪、鸡、牛及羊的粪便。采用琼脂稀释法对分离出的葡萄球菌进行最小抑菌浓度测定。通过PCR方法检测相应耐药基因。【结果】不同动物粪源葡萄球菌对被检抗菌药物耐药严重程度依次为猪粪源葡萄球菌>鸡粪源葡萄球菌>羊粪源葡萄球菌>牛粪源葡萄球菌;鸡粪源葡萄球菌及猪粪源葡萄球菌对苯唑西林、四环素耐药率均超过80%。牛粪源葡萄球菌对庆大霉素和阿米卡星敏感率达100%。ermB基因是猪粪源葡萄球菌和鸡粪源葡萄球菌中检出率最高的林可胺类耐药基因,检出率分别是70.8%和93.5%;femA基因是羊粪源葡萄球菌和牛粪源葡萄球菌中检出率最高的β-内酰胺类耐药基因。【结论】新疆霍城县不同动物粪源葡萄球菌对被检抗菌药物的耐药程度不同,多药耐药情况严重,在养殖过程中应考虑耐药情况需合理使用抗菌药物。

关键词：不同动物粪源葡萄球菌多药耐药耐药基因

抗鸡源EphA2蛋白小鼠多克隆抗体的制备及其特性研究

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中国家禽 2020年第3期42卷 29-34页

作者：姚晓慧李拓凡谢泉万志敏邵红霞秦爱建叶建强扬州大学兽医学院禽类预防医学教育部重点试验室江苏省动物预防医学重点试验室江苏扬州225009 江苏高校动物重要疫病与人兽共患病防控协同创新中心江苏扬州225009 扬州大学农业科技发展研究院(国际联合实验室) 江苏扬州225009 教育部农业与农产品安全国际合作联合实验室江苏扬州225009

为深入探究鸡源EphA2生物学功能,试验首先对鸡源EphA2基因进行了原核分段克隆以及真核表达载体构建。重组原核表达载体分别命名为pGEX-6p-1-EphA2-A(1-534aa)和pGEX-6p-1-EphA2-B(585-972aa),真核表达载体命名为pcDNA3.1-EphA2。重组原... 详细信息

为深入探究鸡源EphA2生物学功能,试验首先对鸡源EphA2基因进行了原核分段克隆以及真核表达载体构建。重组原核表达载体分别命名为pGEX-6p-1-EphA2-A(1-534aa)和pGEX-6p-1-EphA2-B(585-972aa),真核表达载体命名为pcDNA3.1-EphA2。重组原核表达载体转化BL21后经IPTG诱导以及SDS-PAGE分析发现,融合蛋白GST-EphA2-A和GST-EphA2-B均获得有效表达。以纯化的GST-EphA2-A和GST-EphA2-B蛋白免疫小鼠制备了抗鸡EphA2多克隆抗体。间接免疫荧光试验以及Western blot试验表明,所制备的抗鸡EphA2多克隆抗体不仅能识别转染pcDNA3.1-EphA2的DF-1及LMH细胞中表达的外源性EphA2,而且还能有效识别内源性鸡源EphA2蛋白。本研究中鸡EphA2的成功表达及其多克隆抗体研制为后续研究鸡EphA2的功能提供了分子生物学工具。

关键词：鸡源EphA2 原核分段克隆表达真核表达多克隆抗体反应性

Decomposition and Evolution of Intracontinental Strike-Slip Faults in Eastern Tibetan Plateau

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Acta Geologica Sinica(English Edition) 2013年第2期87卷 304-317页

作者： yao Qi XU Xiwei XING Huilin XU Chong WANG xiaohui Key Laboratory of Active Tectonics and Volcano Institute of Geology China Earthquake Administration China Earthquake Networks Center Earth systems Science Computational Centre (ESSCC) The University of Queensland School of Electrical Engineering Beijing Jiaotong University

Little attention had been paid to the intracontinental strike-slip faults of the Tibetan Plateau. Since the discovery of the Longriba fault using re-measured GPS data in 2003, an increasing amount of attention has been paid to this neglected fault. The local relief and transverse swath profile show that the Longriba fault is the boundary line that separates the high and flat tomography of the Tibet plateau from the high and precipitous tomography of Orogen. In addition, GPS data shows that the Longriba fault is the boundary line where the migratory direction of the Bayan Har block changed from eastward to southeastward. The GPS data shows that the Longriba fault is the boundary fault of the sub-blocks of the eastern Bayan Har block. We built three-dimensional models containing the Longriba fault and the middle segment of the Longmenshan fault, across the Bayan Har block and the Sichuan Basin. A nonlinear finite element method was used to simulate the fault behavior and the block deformation of the Eastern Tibetan Plateau. The results show that the low resistivity and low velocity layer acts as a detachment layer, which causes the overlying blocks to move southeastward. The detachment layer also controls the vertical and horizontal deformation of the rigid Bayan Har block and leads to accumulation strain on the edge of the layer where the Longmenshan thrust is located. After a sufficient amount of strain has been accumulated on the Longmenshan fault, a large earthquake occurs, such as the 2008 Wenchuan earthquake. The strike slip activity of the Longriba fault, which is above the low resistivity and low velocity layer, partitions the lateral displacements of the Bayan Har block and adjusts the direction of motion of the Bayan Har block, from the eastward moving Ahba sub-block in the west to southeastward moving Longmenshan sub-block in the east. Four models with different depths to the Longriba fault were constructed：（1） a shallow fault with a depth of only 4 km, （2

关键词： Bayan Har block Longmenshan thrust fault Longriba fault intracontinental fault,nonlinear friction

DNA barcoding commercial Chinese caterpillar fungus

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DNA barcoding commercial Chinese caterpillar fungus

中药与天然药高峰论坛暨第十二届全国中药和天然药物学术研讨会

作者： Li Xiang~a,Jingyuan Song~a,Tianyi Xin~a,Linchun Shi~a,Yingjie Zhu~a,Hui yao~a,xiaohui Pang~a, Shilin Chen~(a,b,*) a National Engineering Laboratory for Breeding of Endangered Medicinal Materials,Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China. b China Academy of Chinese Medical Sciences,Beijing 100700,China.

Chinese caterpillar fungus {Cordyceps sinensis)has been widely and famously used as a precious tonic in the long history of Asian *** the price of *** is higher than that of gold,counterfeits of *** have appeared on the market and are difficult to identify because they are morphologically very similar to *** this study,the DNA barcode ITS/ITS2 was used to distinguish between *** and counterfeits or closely related *** PCR amplification efficiency,differential intra- and inter-specific divergences,DNA barcode gap, and identification ability were used to test the applicability of this DNA barcode.A total of 131 ITS/ITS2 sequences of *** and 19 counterfeits or closely related species were used for the *** results showed that the PCR amplification rate of *** was 100%,the distributions of inter- and intra-specific variation of ITS and ITS2 exhibited distinct barcode gaps, and the identification efficiencies based on the BLAST1,nearest genetic distance,and NJ tree methods were 100%.In summary,the ITS/ITS2 sequence was an ideal DNA barcode to unequivocally distinguish *** from counterfeits or closely related ***,it is also a powerful,although not perfect,tool for identifying the genuine production areas for C. *** barcoding could also be used to ensure the safe use of ***.

关键词： Chinese caterpillar fungus Cordyceps sinensis DNA Barcode ITS/ITS2 Species identification

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被动水下目标识别研究进展综述

无人系统技术 2019年第4期2卷 1-7页

作者：杨宏晖徐光辉李俊豪申昇姚晓辉西北工业大学航海学院

由于海洋环境的复杂性和舰船声隐身技术的发展,被动水下目标识别面临巨大的挑战,是各国水声信号处理领域研究的焦点。首先在介绍基于机器学习的水下目标识别(UATR)技术的基础上,重点分析与总结了水下目标特征提取(时域波形结构特征提取... 详细信息

由于海洋环境的复杂性和舰船声隐身技术的发展,被动水下目标识别面临巨大的挑战,是各国水声信号处理领域研究的焦点。首先在介绍基于机器学习的水下目标识别(UATR)技术的基础上,重点分析与总结了水下目标特征提取(时域波形结构特征提取、频域谱特征提取、时频分析特征提取、听觉感知特征提取)、特征选择、分类器设计等方面的研究现状,然后阐述了在人工智能快速发展背景下的UATR技术发展的现状,最后,论述了UATR智能化发展中依然存在的问题及未来发展趋势。综述表明,在水下目标识别领域,基于舰船辐射噪声的水下目标识别依然面临着诸多困难和挑战,构建智能水下目标识别系统,还需要更多的思考和实践。

关键词：舰船辐射噪声被动水下目标识别特征提取特征选择深度学习类脑智能

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钢轨快速打磨车动力学仿真研究

机械 2024年第4期51卷 27-33页

作者：王振宇章优仕姚晓晖董龙王衡禹西南交通大学牵引动力国家重点实验室四川成都610031 四川西南交大铁路发展股份有限公司四川成都610041

为研究钢轨快速打磨车服役时的动力学性能,建立了考虑打磨小车与大车、磨石与钢轨之间相互作用的钢轨快速打磨车模型,并进行动力学仿真分析,得到了该车在直线和曲线上运行时不同作业状态下的动力学性能。研究表明:钢轨快速打磨车的大车... 详细信息

为研究钢轨快速打磨车服役时的动力学性能,建立了考虑打磨小车与大车、磨石与钢轨之间相互作用的钢轨快速打磨车模型,并进行动力学仿真分析,得到了该车在直线和曲线上运行时不同作业状态下的动力学性能。研究表明:钢轨快速打磨车的大车在自运行、非工作和工作状态下的临界速度分别为178 km/h、176 km/h、169 km/h,打磨小车在非工作和工作状态下的临界速度分别为145 km/h、119 km/h,均能满足80 km/h及以上的速度要求。在不同半径的曲线轨道上运行时,大车和打磨小车的曲线通过性能均随曲线半径的增大而提高,且均能满足标准要求。在不同欠超高量的曲线轨道上运行时,欠超高量越小,打磨小车在作业状态和非作业状态下的曲线通过性能越好,作业状态受欠超高量的影响相较非作业状态较小,且打磨小车的曲线通过安全性在所研究的超高范围内均能满足标准要求。

关键词：钢轨快速打磨车车辆动力学临界速度运行安全性

High-entropy selenides: A new platform for highly selective oxidation of glycerol to formate and energy-saving hydrogen evolution in alkali-acid hybrid electrolytic cell

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Nano Research 2023年第8期16卷 10832-10839页

作者： Hu yao Yibo Wang Yinan Zheng Xin Yu Junjie Ge Yonghong Zhu xiaohui Guo Key Lab of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education The College of Chemistry and Materials ScienceNorthwest UniversityXi’an 710069China Changchun Institute of Applied Chemistry Changchun 130022China School of Chemical Engineering Northwest UniversityXi’an 710069China

Glycerol oxidation reaction(GOR)coupled with hydrogen generation simultaneously is a promising strategy for developing sustainable energy conversion technologies,but the complexity of glycerol oxidation products and the high coupling hydrogen evolution potential limit its wide *** this paper,a self-supported high-entropy selenide electrode can be fabricated via a simple hydrothermal ***,the prepared electrode as an advanced catalyst displays optimal catalytic activity(1.20 V at 10 mA·cm^(−2))and high selectivity for the formation of formate in *** results show that the lattice distortion effect of high entropy materials composed of multiple elements is mainly responsible for the greatly improved catalytic activity and selectivity for ***,an advanced alkali-acid hybrid electrolytic cell was assembled that enables efficient energy-saving hydrogen generation and GOR ***,the electrolyzer requires only 0.5 V applied voltage to reach 10 mA·cm^(−2) for hydrogen generation and maintains long-term operation stability.

关键词： high-entropy selenides lattice distortion glycerol oxidation reaction(GOR) formate activity