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Journal of Oceanology and Limnology 2024年第1期42卷 216-225页

作者： Zhongyi ZHANG Hang LIU xiaohui pan Yanan ZONG Leili FENG Lixian LIU Li GUO Guanpin YANG College of Marine Life Sciences Ocean University of ChinaQingdao 266003China Institutes of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of ChinaQingdao 266003China

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from *** genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional *** random insertional mutant library constructed using this method has become a method of forward genetics for gene ***,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous *** microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries *** this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was *** method will facilitate the genetic studies of ***,which should also be a reference for other microalgal species.

关键词： Nannochloropsis oceanica genetic transformation random insertional mutant library zeocin pretreatment forward genetics

The inhibitory effect of adenosine on tumor adaptive immunity and intervention strategies

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Acta Pharmaceutica Sinica B 2024年第5期14卷 1951-1964页

作者： Longsheng Wang Jie Zhang Wenxin Zhang Mingming Zheng Hongjie Guo xiaohui pan Wen Li Bo Yang Ling Ding Zhejiang Province Key Laboratory of Anti-Cancer Drug Research Institute of Pharmacology and ToxicologyCollege of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China The Innovation Institute for Artificial Intelligence in Medicine Zhejiang UniversityHangzhou 310018China Nanhu Brain-Computer Interface Institute Hangzhou 311100China

Adenosine(Ado)is significantly elevated in the tumor microenvironment(TME)compared to normal *** binds to adenosine receptors(AdoRs),suppressing tumor antigen presentation and immune cell activation,thereby inhibiting tumor adaptive *** downregulates major histocompatibility complex II(MHC II)and co-stimulatory factors on dendritic cells(DCs)and macrophages,inhibiting antigen *** suppresses anti-tumor cytokine secretion and T cell activation by disrupting T cell receptor(TCR)binding and signal *** also inhibits chemokine secretion and KCa3.1 channel activity,impeding effector T cell trafficking and infiltration into the tumor ***,Ado diminishes T cell cytotoxicity against tumor cells by promoting immune-suppressive cytokine secretion,upregulating immune checkpoint proteins,and enhancing immune-suppressive cell *** Ado production in the TME can significantly enhance anti-tumor immune responses and improve the efficacy of other *** and clinical development of inhibitors targeting Ado generation or AdoRs is ***,this article will summarize and analyze the inhibitory effects and molecular mechanisms of Ado on tumor adaptive immunity,as well as provide an overview of the latest advancements in targeting Ado pathways in anti-tumor immune responses.

关键词： Adenosine Adenosine receptors Adenosine-generating enzymes Tumor microenvironment Immune suppression Immune escape Clinical treatment Targeting drugs

反刍动物对饲草不同加工产品高效利用的研究进展

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饲料博览 2024年第1期 20-24页

作者：潘晓辉新疆喀什地区叶城县人民武装部新疆喀什844900

饲草作为反刍动物饲料的主要来源之一,其品质的优劣直接关乎动物的生长发育及生产性能等。因此,在动物养殖过程中常常对饲草进行加工调制以提高其品质和利用率,并制成多种形式的草产品。文章综述了关于不同饲草产品及反刍动物对其高效... 详细信息

饲草作为反刍动物饲料的主要来源之一,其品质的优劣直接关乎动物的生长发育及生产性能等。因此,在动物养殖过程中常常对饲草进行加工调制以提高其品质和利用率,并制成多种形式的草产品。文章综述了关于不同饲草产品及反刍动物对其高效利用方面的研究进展,并为促进畜牧业可持续发展提供相关参考。

关键词：饲草加工调制反刍动物高效利用

基于E2蛋白A-D抗原区检测猪瘟抗体间接ELISA方法的建立与应用

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福建畜牧兽医 2024年第3期46卷 8-11页

作者：潘小慧兆丰华生物科技(福州)有限公司福州350014

本研究以猪瘟病毒E2蛋白主要抗原区E2 A-D为包被抗原,建立一种可特异性检测猪瘟(CSF)抗体的间接ELISA方法。该方法中抗原包被浓度为0.75μg/mL,检测血清稀释倍数为1:150。对123份兔体中和试验测定为阴性的样本进行临界值测定,确定OD450n... 详细信息

本研究以猪瘟病毒E2蛋白主要抗原区E2 A-D为包被抗原,建立一种可特异性检测猪瘟(CSF)抗体的间接ELISA方法。该方法中抗原包被浓度为0.75μg/mL,检测血清稀释倍数为1:150。对123份兔体中和试验测定为阴性的样本进行临界值测定,确定OD450nm≥0.38为阳性,OD450nm≤0.31为阴性,0.31

关键词：猪瘟病毒间接ELISA E2蛋白

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基于CZ-BWT改进的LZW压缩算法在电力报文中的应用

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计算机集成制造系统 2024年第8期30卷 2947-2953页

作者：周航潘小辉孙佳炜腾力阳江结林国网南京供电公司江苏南京210019 南京信息工程大学软件学院江苏南京210044

随着电网远动信息数据量日益剧增,对有限的硬件存储和网络带宽带来严峻考验。通过对报文数据的无损压缩研究以缓解硬件设备压力,确保数据解压后能无损还原出压缩之前的数据显得尤为重要。针对IEC60870-5-104报文特有结构及LZW(Lempel-Zi... 详细信息

随着电网远动信息数据量日益剧增,对有限的硬件存储和网络带宽带来严峻考验。通过对报文数据的无损压缩研究以缓解硬件设备压力,确保数据解压后能无损还原出压缩之前的数据显得尤为重要。针对IEC60870-5-104报文特有结构及LZW(Lempel-Ziv-Welch)的特点,提出基于截断CZ-BWT(Burrows-Wheeler transform)改进的LZW报文压缩算法。首先对输入的IEC60870-5-104报文进行CZ-BWT转换作为预处理,增大报文内容的相关性;其次用LZW算法对转换后的数据进行压缩操作。实验结果表明,基于CZ-BWT改进的LZW算法的压缩率优于传统LZW算法。此外,在压缩和解压速度方面,相比于大部分的压缩算法而言,具有一定优势。

关键词：数据压缩 LZW算法远动信息规约无损压缩

存量规划背景下晋江市陈埭镇低效用地提升策略

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华中建筑 2024年第6期42卷 123-127页

作者：王炜王梓靖潘晓辉福州大学城乡与规划学院福建省城乡规划设计院

“十四五”期间,国土空间规划的改革对国土资源用途进行了严格管制,城镇建设也全面进入存量规划时期,盘活城镇低效用地的研究逐渐兴起。在此背景下,该文选取晋江市陈埭镇为例,剖析其存量用地现状及存在问题,发掘低效用地再开发难点,并... 详细信息

“十四五”期间,国土空间规划的改革对国土资源用途进行了严格管制,城镇建设也全面进入存量规划时期,盘活城镇低效用地的研究逐渐兴起。在此背景下,该文选取晋江市陈埭镇为例,剖析其存量用地现状及存在问题,发掘低效用地再开发难点,并从从调整空间结构、创建产业园区、提高空间品质、重塑文化记忆、健全管理机制等五个方面提出陈埭镇低效用地的提升策略,以期为类似城镇更新提供一些参考。

关键词：存量规划低效用地提升陈埭镇

Transcriptome Analysis During Tetrasporogenesis of Gracilariopsis lemaneiformis and Preliminary Study of the Expressions of Its Meiotic Genes

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Journal of Ocean University of China 2023年第2期22卷 541-554页

作者： ZHU Weifang YANG Ze XU Di YANG Jinxin ZHANG Yingyue pan xiaohui The Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of ChinaQingdao 266003China

The transcriptomes of three different parts of the fertile tetrasporophyte of Gracilariopsis lemaneiformis,including tip(T),middle(M),and subjacent(S)parts,with a gradual tetrasporangium maturity were analyzed and compared to identify the genes involved in the process of *** number of differentially expressed genes(DEGs)for the Gple-S versus Gple-T comparison was 10296,and the numbers of DEGs for the Gple-S versus Gple-M and Gple-T versus Gple-M comparisons were 7435 and 1337,*** Ontology and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed,and the results showed the enrichment of 132 KEGG pathways(corrected P<0.05).A total of 58 DEGs related to meiosis were screened and blasted against 18 meiosis-related genes(dmc1,mlh1,mnd1,msh4,msh2,msh6,mre11,pds5,pms1,rad21,rad50,rad51,smc1,smc2,smc4,smc5,smc6,and spo11),including four meiosis-specific *** transcriptome comparison indicated that in the T part,the meiosis,ribosome,and RNA transport-related genes were mostly up-regulated compared with those in the other two *** the M part,the genes related to ribosomes and the endoplasmic reticulum were also up-regulated compared with those in the lower ***,in the S part,the genes associated with photosynthesis were mostly up-regulated,which might be helpful to the recovery from spore formation and release.

关键词： Gracilariopsis lemaneiformis transcriptome tetrasporogenesis meiosis related genes

小麦-玉米轮作根茬燃烧热触杀防控土传病害的试验

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农业工程学报 2024年第8期40卷 90-97页

作者：朱焕光李刚刘超洁高飞刘新新潘晓慧李鹏飞张晓婷贺超刘亮焦有宙河南农业大学机电工程学院郑州450002 河南农业大学植物保护学院郑州450002 农业农村部农村可再生能源新材料与装备重点实验室郑州450002 河南省低碳农业智能装备工程技术研究中心郑州450002 河南工程学院机械工程学院郑州451191

针对秸秆和根茬直接还田导致作物土传病害重发和频发问题,采用自主设计的可控燃烧热触杀装备进行了试验研究,分析了不同运行工况下装备内的温度分布规律、热触杀后土壤中病原真菌与细菌的灭杀率及作物植株发病程度。结果表明:当进料速度... 详细信息

针对秸秆和根茬直接还田导致作物土传病害重发和频发问题,采用自主设计的可控燃烧热触杀装备进行了试验研究,分析了不同运行工况下装备内的温度分布规律、热触杀后土壤中病原真菌与细菌的灭杀率及作物植株发病程度。结果表明:当进料速度为360~1800 kg/h时,装备可实现热触杀温度(117~167℃)和送风温度(62~172℃)的有效调控,炉膛内沿程烟气温度分布呈先增大后减小趋势,稳定燃烧时炉膛最高温度可达479℃,可保证燃烧效率持续处于较高水平。土壤中细菌比真菌的耐温性更强,117℃下细菌和真菌的灭杀率分别为53.33%和33.33%,132℃以上时两者的灭杀率均达到86%以上,167℃下可实现深度灭杀。与对照组相比,秸秆与根茬燃烧高温热触杀后田间玉米植株根部病情指数降低了34.1,该研究对实现作物连作土传病害的绿色防控具有重要意义。

关键词：作物根茬高温烟气热触杀土传病害病情指数

高密度老城区微绿地选址方法研究——以合肥市老城区为例

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景观设计 2024年第1期22卷 18-21页

作者：潘晓慧钱丰张春彦董英天津大学建筑学院合肥工业大学建筑艺术学院

在现有绿地选址研究的基础上,以微绿地服务水平和居民需求为导向,构建微绿地适建空间评价体系,综合评估居民对现状绿地的获取差异性、不同居住区的需求程度等影响微绿地选址的因素,划定微绿地适建区。通过位置分配(Location-allocation,... 详细信息

在现有绿地选址研究的基础上,以微绿地服务水平和居民需求为导向,构建微绿地适建空间评价体系,综合评估居民对现状绿地的获取差异性、不同居住区的需求程度等影响微绿地选址的因素,划定微绿地适建区。通过位置分配(Location-allocation,LA)模型,在适建区挖掘适建点,并增加居民需求系数以优化LA模型,量化需求差异性的影响。以合肥市老城区为例,进行实地研究,以期为微绿地选址提供新思路。

关键词：微绿地适建空间评价适建点位置分配模型

cDNA cloning of four Hsp genes from Agarophyton vermiculophyllum and transcription analysis in different phases

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Marine Life Science & Technology 2020年第3期2卷 222-230页

作者： xiaohui pan Weifang Zhu Di Xu Hongyan Yang Xiaofei Cao Zhenghong Sui The Key Laboratory of Marine Genetics and Breeding Ministry of EducationCollege of Marine Life SciencesOcean University of China.Qingdao 266003China

Agarophyton vermiculophyllum is an agarophytic red alga originating from Northeast Asia that successfully spread to Europe and North America in the last 20 *** this study,three hsp70 genes(hsp70-l,hsp70-2,and hsp70-3)and one hsp90 gene were cloned,and real-time reverse transcription polymerase chain reaction(RT-qPCR)was used to measure their transcriptional levels in three kinds of thalli(tetrasporophytes,and male/female gametophytes)belonging to diploid or haploid phases in the life cycle of *** results show that the three Hsp70s in *** clustered into three different groups,and the locations of the putative Hsp70-l,Hsp70-2,and Hsp70-3 were in the cytoplasm,endoplasmic reticulum and chloroplast,respectively,according to their *** on the same research,the putative Hsp90 was supposed to have a cytoplasmic *** RT-qPCR results show that the three hsp70 genes were highly upregulated in gametophytes as compared to tetrasporophytes but the transcriptional level of hsp90 did not show such a significant *** chloroplast hsp70-3 exhibited the highest upregulation and the transcriptional level in creased more than 570 fold in female gametophytes,and 17 fold in male gametophytes,compared with ***,cpHsp70-3 might act more like a chaperon molecule involved in haploid development under natural condition,while Hsp70-l and Hsp70-2 were more active in stress resistance.

关键词： Agarophyton vermiculophyllum-Hsp70 Hsp90 Phases RT-qPCR