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Upregulation of GLT25D1 in Hepatic Stellate Cells Promotes Liver Fibrosis via the TGF-β1/SMAD3 Pathway In Vivo and In vitro

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Journal of Clinical and Translational Hepatology 2023年第1期11卷 1-14页

作者： Shiwei Wang Lingling He Fan Xiao Meixin Gao Herui Wei Junru Yang Yang Shu Fuyang Zhang xiaohui ye Ping Li Xiaohua Hao Xingang Zhou Hongshan Wei Department of Gastroenterology Beijing Ditan HospitalCapital Medical UniversityBeijingChina Institute of Infectious Diseases Beijing Ditan HospitalCapital Medical UniversityBeijingChina Department of Gastroenterology Beijing Huaxin Hospitalthe First Affiliated Hospital of Tsinghua UniversityBeijingChina Beijing Ditan Hospital Capital Medical UniversityBeijingChina Department of Pathology Beijing Ditan HospitalCapital Medical UniversityBeijingChina Department of Gastroenterology Peking University Ditan Teaching HospitalBeijingChina

Background and Aims:Collagenβ(1-O)galactosyltransferase 25 domain 1(GLT25D1)is associated with collagen production and glycosylation,and its knockout in mice results in embryonic death.However,its role in liver fibrosis remains elusive,particularly in hepatic stellate cells(HSCs),the primary collagen-producing cells associated with liver fibrogenesis.Herein,we aimed to elucidate the role of GLT25D1 in HSCs.Methods:Bile duct ligation(BDL)-induced mouse liver fibrosis models,primary mouse HSCs(mHSCs),and transforming growth factor beta 1(TGF-β1)-stimulated LX-2 human hepatic stellate cells were used in in vivo and in vitro studies.Stable LX-2 cell lines with either GLT25D1 overexpression or knockdown were established using lentiviral transfection.RNA-seq was performed to investigate the genomic differences.HPLCMS/MS were used to identify glycosylation sites.Scanning electronic microscopy(SEM)and second-harmonic generation/two-photon excited fluorescence(SHG/TPEF)were used to image collagen fibril morphology.Results:GLT25D1 expression was upregulated in nonparenchymal cells in human cirrhotic liver tissues.Meanwhile,its knockdown attenuated collagen deposition in BDL-induced mouse liver fibrosis and inhibited mHSC activation.GLT25D1 was overexpressed in activated versus quiescence LX-2 cells and regulated in vitro LX-2 cell activation,including proliferation,contraction,and migration.GLT25D1 also significantly increased liver fibrogenic gene and protein expression.GLT25D1 upregulation promoted HSC activation and enhanced collagen expression through the TGF-β1/SMAD signaling pathway.Mass spectrometry showed that GLT25D1 regulated the glycosylation of collagen in HSCs,affecting the diameter of collagen fibers.Conclusions:Collectively,the upregulation of GLT25D1 in HSCs promoted the progression of liver fibrosis by affecting HSCs activation and collagen stability.

关键词： Collagen Glycosylation Hepatic stellate cells Liver fibrosis TGF-β1

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MTHFD2在肾透明细胞癌中的表达及其与预后和免疫浸润相关性的分析

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医学理论与实践 2024年第7期37卷 1089-1092页

作者：叶小辉福建省光泽县医院泌尿外科 354100

目的:探究MTHFD2基因表达与肾透明细胞癌(ccRCC)肿瘤进展及免疫浸润的关系,寻找其新的潜在治疗靶点。方法:利用R包(limma,ggplot2,survminer,survival,pROC)及在线数据库,首先分析MTHDF2在ccRCC中的表达水平,随后分析其表达水平与ccRCC... 详细信息

目的:探究MTHFD2基因表达与肾透明细胞癌(ccRCC)肿瘤进展及免疫浸润的关系,寻找其新的潜在治疗靶点。方法:利用R包(limma,ggplot2,survminer,survival,pROC)及在线数据库,首先分析MTHDF2在ccRCC中的表达水平,随后分析其表达水平与ccRCC患者生存期、诊断价值的相关性,最后分析其在ccRCC单细胞表达水平,以及其与免疫细胞浸润和免疫检查点的关系。结果:MTHFD2在ccRCC中表达量增高,且其高表达与肿瘤分期及不良预后相关;在ccRCC肿瘤组织中MTHFD2在CD4^(+)T细胞、CD8^(+)T细胞、调节性T细胞、NK细胞、树突状细胞等多种免疫相关细胞中表达,且其表达量与CD8^(+)T细胞、CD4^(+)T细胞、B细胞及巨噬细胞等免疫细胞及免疫检查点相关。结论:MTHFD2在ccRCC中表达量增多,并与其肿瘤细胞发生发展以及免疫细胞浸润相关,靶向MTHFD2可能是一种潜在的治疗手段。

关键词：肾透明细胞癌 MTHFD2 预后免疫浸润

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Hydrolysis hydrogen production mechanism of Mg10Ni10Ce alloy surface modified by SnO_(2) nanotubes in different aqueous systems

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Green Energy & Environment 2021年第1期6卷 124-137页

作者： Xiaojiang Hou Lu Yang Kaiming Hou Hongchang Shi Lei Feng Guoquan Suo xiaohui ye Li Zhang Yanling Yang School of Material Science and Engineering Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic MaterialsShaanxi University of Science and TechnologyXi'an710021China State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi'an710072China

(Mg-10wt%Ni)-10wt%Ce(Mg10Ni10Ce)was ball-milled with SnO_(2)nanotubes and Mg10Ni10Ce-xSnO_(2)(x¼0,5,10 and 15 wt%)composites have been prepared.The phase compositions,microstructures,morphologies and hydrolysis H2 generation performance in different aqueous systems(distilled water,tap water and simulated seawater)have been investigated and the corresponding hydrolysis mechanism of Mg10Ni10Ce and Mg10Ni10CeeSnO_(2)has been proposed.Adding a small amount of SnO_(2)nanotubes can significantly enhance the hydrolysis reaction of Mg10Ni10Ce,especially the initial hydrolysis kinetics and the final H_(2) generation yield.Unfortunately,the Mg10Ni10Ce-xSnO_(2)hardly reacts with distilled water at room temperature.The hydrolysis reaction rate of Mg10Ni10Cee5SnO_(2)composite in tap water is still very slow with only 17.3%generation yield after 1 h at 303 K.Fortunately,in simulated seawater(3.5 wt%NaCl solution),the hydrolytic H2 generation behavior of the Mg10Ni10Cee5SnO_(2)composite has been greatly improved,which can release as high as 468.6 mL g^(-1 )H_(2) with about 60.9%generation yield within 30 s at 303 K.The Cl destroys the passivation layer on MgeNieCe alloy surface and the added SnO_(2)nanotubes accelerate the hydrolysis reaction rate and enhance the H2 generation yield.The Mg10Ni10Cee5SnO_(2)composite can rapidly generate a large amount of H2 in simulated seawater in a short time,which is expected to be applied on portable H2 generators in the future.

关键词： Mg10Ni10Ce Hydrogen production Surface modification Hydrolysis behavior Aqueous systems

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基于模糊WMR优化PID方法的电机转速控制优化分析

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机械制造与自动化 2024年第2期53卷 239-242页

作者：段晓伟叶小晖江苏省南京市中石化金陵分公司江苏南京210033 清华大学电机工程与应用电子技术系北京100084

针对白噪声下传统PID控制模式造成的输出转速显著波动变化的问题,开发一种通过无刷直流电机转速进行调节的模糊WMR-PID技术。采用模糊推理的方式确定输出变量,根据重心分析法解模糊处理,并建立模糊WMR-PID控制模型。研究结果表明:模糊WM... 详细信息

针对白噪声下传统PID控制模式造成的输出转速显著波动变化的问题,开发一种通过无刷直流电机转速进行调节的模糊WMR-PID技术。采用模糊推理的方式确定输出变量,根据重心分析法解模糊处理,并建立模糊WMR-PID控制模型。研究结果表明:模糊WMR-PID控制方式可以实现快速跟随要求,当初始指令速度为2 450 r/min时,指令在8 s时迅速增大至3 540 r/min,14 s时又迅速降低至1 930 r/min。相对模糊PID方法,模糊WMR-PID能够大幅降低控制动态的超调量,说明模糊WMR-PID控制器可以实现理想的控制效果并达到优异鲁棒性。该研究提高了无刷直流电机转速调节能力,表现出良好控制效果。

关键词：无刷直流电机转速控制模糊自整定超调量鲁棒性

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Construction of SnS2/SnO2 heterostructures with enhanced potassium storage performance

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Journal of Materials Science & Technology 2020年第20期52卷 167-172页

作者： Guoquan Suo Dan Li Lei Feng Xiaojiang Hou xiaohui ye Li Zhang Qiyao Yu Yanling Yang Wei(Alex)Wang School of Materials Science and Engineering Shaanxi University of Science and TechnologyXi’an710021China Institute of Advanced Structure Technology Beijing Institute of TechnologyBeijing 100081China Department of Materials Science and Engineering College of EngineeringPeking UniversityBeijing 100871China

Increasing attention has been focused on potassium ion batteries(KIBs) as promising energy-sto rage system(ESS) owing to the abundance and low-cost of potassium resources.Here,SnS2/SnO2 hete rostructures were successfully fixed onto stainless steel mesh(SnS2/SnO2/SSM) through a facile two-step hydrothermal method and used as anodes for KIBs.Due to the advantages of SnS2/SnO2 heterostructures and good conductivity of SSM substrate,the SnS2/SnO2/SSM anodes display enhanced electrochemical performance.The SnS2/SnO2/SSM anodes deliver specific capacity of 394 mA h g^-1 at 50 mA g^-1 over 100 cycles,better than SnO2/SSM.Even at 500 mA g^-1 after 250 cycles,high capacity of 155 mA h g^-1 can still be obtained.

关键词： SnS2/SnO2 Heterostructures Potassium-ion batteries Anode

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H_2 generation kinetics/thermodynamics and hydrolysis mechanism of high-performance La-doped Mg-Ni alloys in Na Cl solution——A large-scale and quick strategy to get hydrogen

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Journal of Magnesium and Alloys 2021年第3期9卷 1068-1083页

作者： Xiaojiang Hou Hongchang Shi Lu Yang Kaiming Hou Yi Wang Lei Feng Guoquan Suo xiaohui ye Li Zhang Yanling Yang School of Material Science and Engineering Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic MaterialsShaanxi University of Science and TechnologyXi'an 710021China State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi'an 710072China

In this work,La-doped Mg-Ni multiphase alloys were prepared by resistance melting furnace(RMF)and then modified by high-energy ball milling(HEBM).The hydrolysis H_(2) generation kinetics/thermodynamics of prepared alloys in Na Cl solutions have been investigated with the help of nonlinear and linear fitting by Avrami-Erofeev and Arrhenius equations.Combining the microstructure information before and after hydrolysis and thermodynamics fitting results,the hydrolysis H_(2) generation mechanism based on nucleation&growth has been elaborated.The final H_(2) generation capacities of 0La,5La,10La and 15 La alloys are 677,653,641 and 770 m L·g^(-1)H_(2) in 240 min at291 K,respectively.While,the final H_(2) generation capacities of HEBM 0La,5La,10La and 15 La alloys are 632,824,611 and 653 m L·g^(-1)H_(2) in 20 min at 291 K,respectively.The as-cast 15La alloy and HEMB 5La alloy present the best H_(2) production rates and final H_(2) production capacities,especially the HEBM 5La can rapidly achieve high H_(2) generation capacity(670 and 824 m L·g^(-1)H_(2) )at low temperature(291 K)within short time(5 and 20 min).The difference between the H_(2) generation capacities is mainly originated from the initial nucleation rate of Mg(OH)_(2) and the subsequent processes affected by the microstructures and phase compositions of the hydrolysis alloys.Relative low initial nucleation rate and fully growth of Mg(OH)_(2) nucleus are the premise of high H_(2) generation capacity due to the hydrolysis H_(2) generation process consisted by the nucleation,growth and contacting of Mg(OH)_(2) nucleus.To utilization H_(2) by designing solid state H_(2) generators using optimized Mg-based alloys is expected to be a feasible H_(2) generation strategy at the moment.

关键词： H_(2)generation La-doped Mg-Ni alloys Kinetics Thermodynamics Hydrolysis mechanism

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Full-spectrum responsive photocatalytic activity via non-noble metal Bi decorated mulberry-like BiVO_(4)

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Journal of Materials Science & Technology 2021年第24期83卷 102-112页

作者： Yaxin Bi Yanling Yang Xiao-Lei Shi Lei Feng Xiaojiang Hou xiaohui ye Li Zhang Guoquan Suo Siyu Lu Zhi-Gang Chen School of Materials Science and Engineering Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic MaterialsShaanxi University of Science and TechnologyXi'an710021China Centre for Future Materials University of Southern QueenslandSpringfieldQLD4300Australia School of Mechanical and Mining Engineering The University of QueenslandBrisbaneQLD4072Australia College of Chemistry and Molecular Engineering Zhengzhou UniversityZhengzhou 450001China

Due to its appropriate bandgap(~2.4 e V)and efficient light absorption,bismuth vanadate(Bi VO_(4))shows promising photocatalysis activity.However,the charge carrier recombination and poor electron transmission often induce poor photocatalytic performance.Herein,we report a new method to in-situ synthesize non-noble metal Bi decorated mulberry-like Bi VO_(4)by a two-step calcination process.Comprehensive characterizations reveal that non-noble metal Bi nanoparticles grown in-situ on Bi VO_(4)result in the red-shift of the absorbance edge,greatly extending the light absorption from the ultraviolet into the near-infrared region.The surface plasmon resonance excitation of Bi nanoparticles and synergetic effects between Bi and Bi VO_(4)effectively improve the photocatalytic efficiency and promote the separation of photoinduced electron-hole pairs in mulberry-like Bi VO_(4).Density functional theory(DFT)calculation results further verify that the electrons are transferred from Bi to Bi VO_(4)and the formation of·OH radical in Bi/Bi VO_(4)is attributed to the lower simulated free energy,which supports our experimental outcomes.This work provides a novel strategy to enhance light absorption and promote efficient solar utilization of photocatalysts for practical applications.

关键词： Bi BiVO_(4) Photocatalysis Full-spectrum Density functional theory

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Laser-ablated violet phosphorus/graphene heterojunction as ultrasensitive ppb-level room-temperature NO sensor

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中国化学快报:英文版 2023年第9期34卷 248-253页

作者： xiaohui ye Ming Qi Hao Qiang Mengzhen Chen Xi Zheng Mengyue Gu Xuewen Zhao Yanling Yang Chaozheng He Jinying Zhang School of Material Science and Engineering Shaanxi University of Science and TechnologyXi’an 710021China Center of Nanomaterials for Renewable Energy(CNRE) School of Electrical EngineeringXi’an Jiaotong UniversityXi’an 710049China Institute of Environmental and Energy Catalysis School of Materials Science and Chemical EngineeringXi’an Technological UniversityXi’an 710021China

Two dimensional(2D)materials are promising gas sensing materials,but the most of them need to be heated to show promising sensing performance.Sensing structures with high sensing performance at room-temperature are urgent.Here,another 2D material,violet phosphorus(VP)nanoflake is investigated as gas sensing material.The VP nanoflakes have been effectively ablated to have layers of 1–5 layers by laser ablation in glycol.The VP nanoflakes are combined with graphene to form VP/G heterostructuresbased NO sensor.An ultra-high gauge factor of 3×10^(7)for ppb-level sensing and high resistance response of 59.21%with ultra-short recovery time of 6s for ppm-level sensing have been obtained.The sensing mechanism is also analysed by density functional theory(DFT)calculations.The adsorption energy of VP/G is calculated to be-0.788 e V,resulting in electrons migration from P to N to form a P-N bond in the gap between VP and graphene sheet.This work provides a facile approach to ablate VP for mass production.The as-produced structures have also provided potential gas sensors with ultrasensitive performance as ppb-level room-temperature sensors.

关键词： Laser ablation Violet phosphorus nanoflakes Heterostructures ppb level NO sensor

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Thermoelectric performance enhancement by manipulation of Sr/Ti doping in two sublayers of Ca_(3)Co_(4)O_(9)

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Journal of Advanced Ceramics 2020年第6期9卷 769-781页

作者： Li ZHANG Yichen LIU Thiam Teck TAN Yi LIU Jian ZHENG Yanling YANG Xiaojiang HOU Lei FENG Guoquan SUO xiaohui ye Sean LI Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic Materials School of Materials Science and EngineeringShaanxi University of Science&TechnologyXi’an 710021China UNSW Materials and Manufacturing Futures Institute School of Material Science and EngineeringThe University of New South WalesKensingtonNew South Wales2052Australia

Thermoelectric(TE)performance of Ca_(3)Co_(4)O_(9)(CCO)has been investigated extensively via a doping strategy in the past decades.However,the doping sites of different sublayers in CCO and their contributions to the TE performance remain unrevealed because of its strong correlated electronic system.In this work,Sr and Ti are chosen to realize doping at the[Ca_(2)CoO_(3)]and[CoO_(2)]sublayers in CCO.It was found that figure of merit(ZT)at 957 K of Ti-doped CCO was improved 30% than that of undoped CCO whereas 1 at% Sr doping brought about a 150% increase in ZT as compared to undoped CCO.The significant increase in electronic conductivity and the Seebeck coefficient are attributed to the enhanced carrier concentration and spin-entropy of Co^(4+) originating from the Sr doping effects in[Ca_(2)CoO_(3)]sublayer,which are evidenced by the scanning electron microscope(SEM),Raman,Hall,and X-ray photoelectron spectroscopy(XPS)analysis.Furthermore,the reduced thermal conductivity is attributed to the improved phonon scattering from heavier Sr doped Ca site in[Ca_(2)CoO_(3)]sublayer.Our findings demonstrate that doping at Ca sites of[Ca_(2)CoO_(3)]layer is a feasible pathway to boost TE performance of CCO material through promoting the electronic conductivity and the Seebeck coefficient,and reducing the thermal conductivity simultaneously.This work provides a deep understanding of the current limited ZT enhancement on CCO material and provides an approach to enhance the TE performance of other layered structure materials.

关键词： layered structures manipulation doping sites Ca_(3)Co_(4)O_(9)(CCO) spin-entropy thermoelectric performance

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不同灭菌方式对细胞毒性评价的研究

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安徽预防医学杂志 2024年第1期30卷 61-65页

作者：叶小恵张磊章亚兵安徽省食品药品检验研究院安徽合肥230051

目的探讨不同灭菌方式对4种医疗器械或材料细胞毒性评价结果的影响。方法采用浸提液法和MTT法2种细胞毒性方法对4种常用医疗器械和材料(一次性使用医用口罩、一次性使用多功能手术解剖器(镊体)、注射用冷冻干燥用氯化丁基橡胶塞和一... 详细信息

目的探讨不同灭菌方式对4种医疗器械或材料细胞毒性评价结果的影响。方法采用浸提液法和MTT法2种细胞毒性方法对4种常用医疗器械和材料(一次性使用医用口罩、一次性使用多功能手术解剖器(镊体)、注射用冷冻干燥用氯化丁基橡胶塞和一次性使用输液器)在不同灭菌方式(紫外辐照灭菌、高温湿热灭菌、环氧乙烷灭菌)后的细胞毒性进行测定。结果一次性使用医用口罩、一次性使用多功能手术解剖器(镊体)、注射用氯化丁基橡胶塞在3种灭菌条件下细胞毒性分级均为1级,细胞存活率均>70%;一次性使用输液器在紫外辐照灭菌和高温湿热灭菌方式下细胞毒性分级均为1级,细胞存活率均>70%,环氧乙烷灭菌条件下细胞毒性分级为2级,细胞存活率<70%。结论医疗器械或材料的灭菌方式会影响其细胞毒性结果的测定,根据产品理化性质和材料的不同,应选择适宜的灭菌方式。尤其多组分、多材料,涉及各种添加剂的器械产品,在进行细胞毒性试验时一定要根据产品的特性或与其临床使用灭菌方式保持一致。

关键词：紫外辐照灭菌高温湿热灭菌环氧乙烷灭菌细胞毒性

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