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Prevention of DNA re-replication in eukaryotic cells

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Journal of Molecular Cell Biology 2011年第1期3卷 13-22页

作者： Lan N.Truong xiaohua wu Department of Molecular and Experimental Medicine The Scripps Research InstituteLa JollaCA 92037USA

DNA replication is a highly regulated process involving a number of licensing and replication factors that function in a carefully orchestrated manner to faithfully replicate DNA during every cell *** of proper licensing control leads to deregulated DNA replication including DNA re-replication,which can cause genome instability and *** organisms have established several conserved mechanisms to prevent DNA re-replication and to counteract its potentially harmful *** mechanisms include tightly controlled regulation of licensing factors and activation of cell cycle and DNA damage *** licensing control and its associated compromised checkpoints have both been observed in tumor cells,indicating that proper functioning of these pathways is essential for maintaining genome *** this review,we discuss the regulatory mechanisms of licensing control,the deleterious consequences when both licensing and checkpoints are compromised,and present possible mechanisms to prevent re-replication in order to maintain genome stability.

关键词： DNA re-replication cell cycle checkpoints DNA damage response Cdt1 DSB repair genome stability tumorigenesis

p38γ regulates UV-induced checkpoint signaling and repair of UV-induced DNA damage

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Protein & Cell 2010年第6期1卷 573-583页

作者： Chia-Cheng wu xiaohua wu Jiahuai Han Peiqing Sun Department of Immunology and Microbial Science The Scripps Research Institute10550 N.Torrey Pines Road.La JollaCA 92037USA Department of Molecular and Experimental Medicine The Scripps Research Institute10550 N.Torrey Pines Road.La JollaCA 92037USA Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering School of Life SciencesXiamen UniversityXiamen 361005China Dempartment of Molecular Biology The Scripps Research Institute10550 N.Torrey Pines Road.La JollaCA 92037USA

In eukaryotic cells,DNA damage triggers activation of checkpoint signaling pathways that coordinate cell cycle arrest and repair of damaged *** DNA damage responses serve to maintain genome stability and prevent accumulation of genetic mutations and development of *** p38 MAPK was previously implicated in cellular responses to several types of DNA ***,the role of each of the four p38 isoforms and the mechanism for their involvement in DNA damage responses remained poorly *** this study,we demonstrate that p38γ,but not the other p38 isoforms,contributes to the survival of UV-treated *** of p38γsensitizes cells to UV exposure,accompanied by prolonged S phase cell cycle arrest and increased rate of *** investigation reveal that p38γis essential for the optimal activation of the checkpoint signaling caused by UV,and for the efficient repair of UVinduced DNA *** findings have established a novel role of p38γin UV-induced DNA damage responses,and suggested that p38γcontributes to the ability of cells to cope with UV exposure by regulating the checkpoint signaling pathways and the repair of damaged DNA.

关键词： p38γ DNA damage UV DNA repair checkpoint signaling

Electronic Auction Scheme Based on Smart Contract and IPFS

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计算机工程 2023年第2期49卷 181-190页

作者： wu xiaohua LIU Huan wu Fengheng ZHANG Ke School of Information and Software Engineering University of Electronic Science and Technology of China Chengdu Huanhaishaqiu Network Technology Co. Ltd.

Sealed-bid auctions are a vital transaction tool in the e-commerce *** centralized auction schemes typically result in severe threats to data integrity,information transparency,and traceability owing to their excessive reliance on third parties,and blockchain-based auction schemes generally suffer from high storage costs and are deficient in functional and architectural *** solve these problems,this study presents a sealed-bid auction scheme that removes the third-party based on an Ethereum smart contract,ensuring data integrity,openness,and transparency in the execution *** commitment mechanism and distributed storage system help to significantly reduce the user’s storage cost and protect the privacy of user *** the functional design,this study introduces a fulltext-retrieval and dispute-processing module for commodities,which reduces the defects existing in the functional module design of existing auction ***,a prototype auction system on the Ethereum test chain is built to validate the proposed *** show that compared with traditional storage methods,indirect storage based on a distributed storage system of texts and images can reduce the storage cost by at least 50%while ensuring data ***,the gas cost at each stage of the auction scheme and the time required for the full-text retrieval of products are recorded to evaluate the scheme performance and analyze the test results.

关键词： sealed bid auction Ethereum smart contract commitment Interplanetary File System(IPFS)

An Efficient Method for Sulfonylation of Amines, Alcohols and Phenols with N-Fluorobenzenesulfonimide Under Mild Conditions

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Chemical Research in Chinese Universities 2018年第2期34卷 191-196页

作者： JING Li YU Xinling GUAN Mei wu xiaohua WANG Qiantao wu Yong Key Laboratory of Drug-targeting of Education Ministry Department of Medicinal Chemistry West China School of Pharmacy Sichuan University Chengdu 610041 P. R. China West China Hospital Sichuan University Chengdu 610041 P. R. China

A new and convenient procedure was developed for the preparation of sulfonamides and sulfonic esters using N-fluorobenzenesulfonimide（NFSI） as a novel phenylsulfonyl group transfer reagent. In this protocol, a broad range of functional groups were tolerated to give the corresponding sulfonamides or sulfonic esters in moderate to excellent yields. The synthetic strategy for sulfonamides formation proceeded efficiently even under base-, metal- and additive-free conditions with the advantages of operational simplicity, mild reaction conditions as well as short reaction time.

关键词： N-Fluorobenzenesulfonimide Sulfonylation Sulfonamide Sulfonic ester

A Deep Learning-Enabled Skin-Inspired Pressure Sensor for Complicated Recognition Tasks with Ultralong Life

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Research 2024年第1期2023卷 443-454页

作者： Yingxi Xie xiaohua wu Xiangbao Huang Qinghua Liang Shiping Deng Zeji wu Yunpeng Yao Longsheng Lu School of Mechanical&Automotive Engineering South China University of TechnologyGuangzhouChina

Flexible full-textile pressure sensor is able to integrate with clothing directly,which has drawn extensive attention from scholars *** the realization of flexible full-textile pressure sensor with high sensitivity,wide detection range,and long working life remains *** recognition tasks necessitate intricate sensor arrays that require extensive data processing and are susceptible to *** human skin is capable of interpreting tactile signals,such as sliding,by encoding pressure changes and performing complex perceptual *** by the skin,we have developed a simple dip-and-dry approach to fabricate a full-textile pressure sensor with signal transmission layers,protective layers,and sensing *** sensor achieves high sensitivity(2.16 kPa^(−1)),ultrawide detection range(0 to 155.485 kPa),impressive mechanical stability of 1 million loading/unloading cycles without fatigue,and low material *** signal transmission layers that collect local signals enable real-world complicated task recognition through one single *** developed an artificial Internet of Things system utilizing a single sensor,which successfully achieved high accuracy in 4 tasks,including handwriting digit recognition and human activity *** results demonstrate that skin-inspired full-textile sensor paves a promising route toward the development of electronic textiles with important potential in real-world applications,including human–machine interaction and human activity detection.

关键词： Deep Skin utilizing

Application of simulated annealing genetic algorithm-optimized back propagation(BP)neural network in fault diagnosis

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International Journal of Modeling, Simulation, and Scientific Computing 2019年第4期10卷 84-95页

作者： Dawei Zhang Weilin Li xiaohua wu Xiaofeng Lv School of Automation Department of Electrical Engineering Northwestern Polytechnical University 710129 Xi’an ShaanxiP.R.China School of Basic Science for Aviation Naval Aviation University 264001 Yantai ShandongP.R.China

Optimal weights are usually obtained in neural network through a fixed network conformation,which affects the practicality of the *** at the shortage of conformation design and weight training algorithm in neural network application,the back propagation(BP)neural network learning algorithm combined with simulated annealing genetic algorithm(SAGA)is put *** multi-point genetic optimization of neural network topology and network weights is performed using hierarchical coding schemes and genetic *** simulated annealing mechanism is incorporated into the Genetic Algorithm(GA)to optimize the design and optimization of neural network conformation and network weights *** SAGA takes advantage of GA excellent ability in grasping the overall ability of the search process,also uses the SA algorithm to control the convergence of the algorithm to avoid premature *** fault diagnosis of one certain on-board electrical control box of helicopter and one certain flight control box of aircraft autopilot were used as a test platform to simulate the *** simulation conclusions reveal that the algorithm has good convergence rate and high diagnostic accurateness.

关键词： Neural network genetic algorithm simulated annealing algorithm on-board electrical control box fault diagnosis

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嫁接对瓠瓜抗病抗逆性及果实品质的影响

核农学报 2025年第1期39卷 191-200页

作者：王海燕汪颖王尖邢乃林李国景徐沛吴晓花中国计量大学生命科学学院浙江杭州310018 浙江省农业科学院蔬菜研究所浙江杭州310021 宁波市特色园艺作物品质调控和抗性育种重点实验室浙江宁波315040

为探究嫁接对瓠瓜抗病抗逆性和果实品质的影响,以瓠瓜自根苗为对照,比较4种南瓜型砧木(全能铁甲、思壮12、砧西316、黑武士)嫁接浙蒲9号瓠瓜的成活率、枯萎病抗性、低温弱光性及耐盐性、果实品质等差异,并通过主成分分析及隶属函数法对... 详细信息

为探究嫁接对瓠瓜抗病抗逆性和果实品质的影响,以瓠瓜自根苗为对照,比较4种南瓜型砧木(全能铁甲、思壮12、砧西316、黑武士)嫁接浙蒲9号瓠瓜的成活率、枯萎病抗性、低温弱光性及耐盐性、果实品质等差异,并通过主成分分析及隶属函数法对不同嫁接组合进行综合评价。结果表明,浙蒲9号/思壮12嫁接组合的成活率最高(94.87%),不同砧穗组合枯萎病抗性均较对照增强。浙蒲9号/思壮12在低温弱光下的最大光化学效率(Fv/Fm)最高,在200 mmol·L^(-1)NaCl胁迫下生长较好。与自根苗相比,嫁接植株长势均变旺,果实的维生素C含量降低,其他指标均有不同程度的提升。浙蒲9号/全能铁甲的果实可溶性固形物含量最高,浙蒲9号/砧西316的果实钙含量最高,浙蒲9号/黑武士的果实蛋白质和镁含量最高。浙蒲9号/思壮12的产量、果实游离谷氨酸和钾含量最高,硬度和可滴定酸含量最小,瓠瓜品质有所提升。同时,主成分分析和隶属函数值综合评价结果表明,浙蒲9号/思壮12的D值为0.914,排名第一。综上,嫁接可以增强瓠瓜枯萎病抗性,不同程度提高瓠瓜的低温、盐胁迫耐受性,合理选择砧木可提高瓠瓜果实品质,以思壮12为砧木嫁接浙蒲9号瓠瓜综合表现最佳。本研究结果为适宜瓠瓜嫁接砧木的选择提供了技术支持。

关键词：瓠瓜嫁接抗病抗逆性果实品质综合评价

An Electronic Auction Scheme Based on Smart Contract and IPFS

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计算机工程 2022年

作者： xiaohua wu Huan Liu Fengheng wu Ke Zhang School of Information and Software Engineering，University of Electronic Science and Technology of China Chengdu Sea&Dune Network Technology Co.， LTD

Sealed-bid auctions are a vital transaction tool in the field of e-commerce. Through the investigation of the current sealed bid auction scheme， this paper finds that there are still many problems to be solved. The traditional centralized auction scheme has severe threats to data integrity， information transparency， and traceability due to its excessive reliance on third parties. Blockchain-based auction schemes generally suffer from high storage costs and are still not perfect in function and architectural design. In order to solve the above problems， this paper proposes a sealed bidding auction scheme that removes the third party based on the Ethereum smart contract， which ensures the integrity of the data and the openness and transparency of the execution process. Through the Commitment mechanism and distributed storage system， the user's storage cost is significantly reduced while protecting the privacy of user bids. In terms of functional design， a full-text retrieval and dispute processing module for commodities is added， which improves the defects existing in the functional module design of the current auction system. Furthermore， we built a prototype auction system on the Ethereum test chain to validate the proposed scheme. The experimental results show that， compared with traditional storage methods， indirect storage of text and pictures through a distributed storage system can reduce the storage cost of users by at least 50% while ensuring data integrity. Finally， we test the gas cost of each stage of the auction scheme and the time-consuming product full-text retrieval to evaluate the scheme's performance and give the test results and analysis evaluation.

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Preparation of Fe-Based Bulk Amorphous Materials and Its Application to Sensors

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Journal of Rare Earths 2004年第Z1期22卷 90-92页

作者： Sun xiaohua wu Jinbin Zhao Zengqi Zhao Jun Sun Guangjie Liu Changsheng School of Materials &Metallurgy Northeastern UniversityShenyang 110004 China Baotou Rare Earth Research Institute Baotou 014010 China Baotou Rare Earth Research Institute Baotou 014010 China Baotou Rare Earth Research Institute Baotou 014010 China Baotou Rare Earth Research Institute Baotou 014010 China Baotou Rare Earth Research Institute Baotou 014010 China School of Materials &MetallurgyNortheastern UniversityShenyang 110004 China

The study of recent years found that big bulk amorphous alloys were formed for some multi-element compositions at rapid cooling speed such as Zr-, La-, Fe-, Mg-based alloys with wide undercooled liquid phase field and high trend of forming glass.( ATx = crystallization temperature Tx - glass transformation temperature Tg) Bulk amorphous copper mold upper suction casting with minus pressure while some new technical processes and adding new elements such as Co, Nb, Ca, *** used to improve magnetic properties and other performances of the *** results show that Fe-based bulk amorphous alloys have low coercive force and high permeability, which are successfully applied to magnetoelectric sensors with temperature ranges between -45 ℃ and 150 ℃ by special design of magnetic circuits.

关键词： bulk amorphous glass transformation ability suction casting of minus pressure sensor