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SiRAP2-12,a Positive Regulatory Factor,Effectively Improves the Waterlogging Tolerance of Foxtail Millet(Setaria italica)

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 445-465页

作者： Xueyan Xia xiaohong fu Yu Zhao Jihan Cui Nuoya Xiao Jingxin Wang Yiwei Lu Meihong Huang Cheng Chu Jia Zhang Mengxin Yang Shunguo Li Jianfeng Liu Institute of Millet Crops Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Genetic Improvement and Utilization for Featured Coarse Cereals(Co-Construction byMinistry and Province)Ministry of Agriculture and Rural Affairs/The Key Research Laboratory of Minor Cereal Crops of Hebei ProvinceShijiazhuang050035China School of Life Sciences Institute of Life Science and Green DevelopmentHebei UniversityBaoding071002China

Foxtail millet(Setaria italica)growth was inhibited because of waterlogging stress,which has caused yield *** family plays an important role to plant adversity *** our study,we obtained 19,819 differential expressed genes(DEGs)between the two treatments based on the RNA-seq sequencing of foxtail millet of water-logging ***,a total of 28 ERF family members were obtained,which have a complete open read-ing *** studied the evolution and function of SiERF family and how they affected the waterlogging *** was found that SiERF1A/B/C(GenBank ID:OR775217,OR775219,OR775218)and SiRAP2-12(GenBank ID:OR775216)have similar functions to the known waterlogging tolerance genes of other *** them,the SiRAP2-12 expression was obviously signiﬁcantly up-regulated in foxtail millet after 5d water-logging *** SiRAP2-12 was silenced,the activity of defense enzymes in millet decreased signiﬁ*** details,superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),the osmotic regulator proline(Pro),and the activity of the anaerobic respiratory enzyme alcohol dehydrogenase(ADH)content were decreased by 78.61%,29.52%,79.95%,19.41%and 54.77%,*** contrast,the relative electrical conductivity contents(REC),malondialdehyde(MDA),and hydrogen peroxide(H_(2)O_(2))of the foxtail millet subjected to virus-induced gene silencing clearly increased by 1.03-fold,36.09%,and 15.21%,*** content of sodium(Na^(+))in the SiRAP2-12-silenced foxtail millet also increased,but that of potassium(K^(+))***,we found that ethylene content was signiﬁcantly ***,the SiAOC1 expression,an essential gene for ethylene synthesis,was inhibited in SiRAP2-12-silenced foxtail millet after waterlogging *** together,we hypothesized that SiRAP2-12 might be a positive regulator of millet tolerance to waterlogging stress.

关键词： Foxtail millet waterlogging SiRAP2-12 VIGS ethylene

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情动的身体拉美魔幻现实主义电影的美学辩证法

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北京电影学院学报 2024年第3期 120-128页

作者：付晓红杨宾中国传媒大学戏剧与影视学院北京外国语大学中华文化国际传播研究院

以“魔幻现实主义”命名的拉美电影,由现实主义转向魔幻现实主义的同时,也由“纪录+批判现实”转向用“象征+奇观”表现精神世界,由政治表达转向情动(affect)表述。通过马尔克斯编剧的电影,索拉纳斯、佐杜洛夫斯基导演的电影为研究对象... 详细信息

以“魔幻现实主义”命名的拉美电影,由现实主义转向魔幻现实主义的同时,也由“纪录+批判现实”转向用“象征+奇观”表现精神世界,由政治表达转向情动(affect)表述。通过马尔克斯编剧的电影,索拉纳斯、佐杜洛夫斯基导演的电影为研究对象,可以发现拉美魔幻现实主义电影以“情动身体”为核心意象,将拉美历史记忆与现代经验的纷乱经纬交织,将这片土地所共有的情动传统相调和,形成独具拉美电影特色的“美学辩证法”。

关键词：魔幻现实主义拉丁美洲新电影情动身体

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Effects of vanadium(V)-substitution on the oxidative properties of α-Keggin-type heteropolyanion clusters——progress in DFT theoretical studies

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Science China Chemistry 2009年第12期52卷 2096-2105页

作者： WANG JinYue fu xiaohong WANG Jian HU ChangWei Institute of Applied Chemistry Department of Chemistry and Chemical EngineeringKey Laboratory of Computational Physics in Universities of SichuanYibin UniversityYibin 644007China Key Laboratory of Green Chemistry and Technology Ministry of EducationCollege of ChemistrySichuan UniversityChengdu 610064China

The redox properties of α-Keggin-type heteropolyanion clusters [XM12O40]n-(X = Si,P; M = Mo,W) mainly depend on their constituent outer metal-oxygen cages {M12O36}. They act as "reservoirs",through which the transfer and transition of electrons and protons may occur. At the atomic and molecular level,the redox properties of these clusters can be controlled and also tuned by modifying the metal M in the cages and the central heteroatom X of the clusters. Combined with relevant experimental results,this review summarizes our recent theoretical investigations of the effect of vanadium substitution on the redox properties of Keggin anion clusters. Theoretical modeling and calculation results showed that the oxidative ability of the modified species was increased by partial substitution of the cage M atoms of the Keggin clusters by vanadium atoms which have lower electronegativity. A linear correlation between the catalytic efficiency per vanadium atom and the microstructures of the vanadium(V)-substituted heteropolyanions [PVnMo12-nO40](3+n)-(n = 1-3) was established for the first time. This relationship may be suitable to interpret the catalytic behavior of the title compounds in the hydroxylation of benzene to phenol,and may also be used in understanding other reactions such as the oxidative dehydrogenation of isobutyric acid and the nitration of adamantine. The establishment of this nearly linear structure-property relationship may lay the foundations of understanding the behavior of the title compounds in homogeneous catalytic oxidation reactions,and may direct the design of future catalysts and the choice of other catalytic reactions.

关键词： polyoxometalates α-Keggin-type heteropolyanions vanadium substitution oxidative property density functional theory (DFT)

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高效液相色谱-质谱法同时测定退热贴中13种解热镇痛类化学药物

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化学分析计量 2024年第6期33卷 34-39页

作者：傅小红陈思姜伟张思甜南昌市检验检测中心南昌330000

建立了高效液相色谱-质谱法同时测定退热贴中13种解热镇痛类化学药物。样品用5 mmol/L甲酸铵溶液(含0.1%的甲酸)-甲醇溶液(体积比为1∶1)提取,经Waters ACQUITY UPLC BEH C_(18)色谱柱(100 mm×2.1 mm,1.7μm)梯度洗脱分离,采用电喷雾... 详细信息

建立了高效液相色谱-质谱法同时测定退热贴中13种解热镇痛类化学药物。样品用5 mmol/L甲酸铵溶液(含0.1%的甲酸)-甲醇溶液(体积比为1∶1)提取,经Waters ACQUITY UPLC BEH C_(18)色谱柱(100 mm×2.1 mm,1.7μm)梯度洗脱分离,采用电喷雾离子源,多反应监测模式进行检测,以色谱峰面积外标法进行定量。13种化合物的质量浓度在各自浓度范围内与色谱峰面积呈良好的线性关系,相关系数为0.995~0.9996,方法检出限为0.12~2.5μg/kg,定量限为0.32~8.2μg/kg;基质空白加入不同浓度水平的加标回收率为93.9%~102.0%,测定结果的相对标准偏差为2.31%~4.81%(n=6)。该方法基质干扰小、准确、可靠,适用于退热贴中解热镇痛类化学药非法添加的测定。

关键词：退热贴解热镇痛非法添加高效液相色谱-质谱法

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Effect of SiC whiskers on the oxidation protective properties of SiC coatings for carbon/carbon composites

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Rare Metals 2006年第1期25卷 58-62页

作者： SHI xiaohong LI Hejun fu Qiangang LI Kezhi ZHANG Xiulian The Key Laboratory of Superhigh Temperature Composites Northwestern Polytechnical University Xi＇an 710072 China

In order to effectively employ the unique high temperature mechanical properties of carbon/carbon composite substrates, SiC coatings reinforced by SiC whiskers were prepared by pack cementation method. The effect of SiC whiskers on the oxidation resistance properties of the single-layer coating and double-layer coating was investigated. SiC whiskers in the single-layer SiC coating have little effect on the anti-oxidation property but obviously improve the thermal shock property. The double-layer coating with inner-layer reinforced coating exhibits more perfect anti-oxidation ability than the double-layer coating with SiC inner-layer coating.

关键词： carbon/carbon composites SiC coating oxidation protection SiC whiskers

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Effects of glutamate and nimodipine on survival rate of embryonic rat neuronal stem cells cultured in vitro

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Neural Regeneration Research 2008年第12期3卷 1286-1289页

作者： xiaohong Lu Hailong fu Qiang Sun Li Cui Dihui Ma Weihong Lin Department of Neurology First Hospital Jinlin University Changchun 1300021 Jilin Province China

BACKGROUND： At least three types of calcium ion channel （T, N, and L） have been recognized in nerve ceils, but only the L type of channel is sensitive to drugs. Theoretically, nimodipine can lead to L-type channel inactivation and prevent calcium ion inflow, thereby exhibiting protective effects on nerve cells. OBJECTIVE： To observe the protective effects of nimodipine on glutamate-induced injury to embryonic rat neural stem cells, and to make a comparison with MK-801, a nonselective glutamate receptor antagonist. DESIGN, TIME AND SETTING： The present in vitro experiment pertaining to neural stem cells was performed at the Department of Neurology, First Hospital, Jilin University between January 2005 and December 2006. MATERIALS： Glutamate was sourced from the Shanghai Biological Research Institute of the Chinese Academy of Sciences. Nimodipine was provided by Bayer Company, Germany. Brain tissue was taken from Wistar rats on day 15 of gestation for isolation and culture of neural stem cells. METHODS： Passage 2 neural stem cell spheres were taken for preparation of single cell suspension. The prepared single cell suspension was divided into 4 groups：（1） Normal control, normally cultured. （2） Glutamate, cultured with 50, 100, 200, 500, and 1 000 μmol/L glutamate. （3） Nimodipine, received a 30- minute nimodipine [1×（10^-8-10^-2） g/L] culture followed by a glutamate （200 μmol/L） treatment step. （4） MK-801, given as 30- minute MK-801 （100 μmol/L） culture, followed by a glutamate （200 μmol/L） treatment step. MAIN OUTCOME MEASURES： Determination of glutamate-induced cell death by methyl thiazolyl tetrazolium （MTT） assay; calculation of neural stem cell survival rate following addition of nimodipine. RESULTS： The survival rate of neural stem cells was approximately 25.26% following 24 hour 50 μmol/L glutamate culture and gradually decreased as the glutamate dose increased （P 〈 0.05 0.01）. Only 9.27% of neural stem cells survived when the glutamate dose was 1000 μmol/L. Th

关键词： neural stem cells culture glutamate injury nimodipine protective effects

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甘草泻心口腔护理液改善脑卒中患者口腔状态的临床研究

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国际医药卫生导报 2024年第17期30卷 2974-2978页

作者：施华符晓红王乐苏亚盼张倬萁河南中医药大学第一附属医院神经外科郑州450000 河南科技大学第一附属医院肝胆外科洛阳471003

目的探讨甘草泻心口腔护理液(甘草泻心口护液)改善脑卒中患者口腔状态的效果。方法采用随机对照试验,选取2021年12月至2023年10月在河南中医药大学第一附属医院神经外科治疗的80例脑卒中患者作为研究对象,以随机数表法分为对照组和观察... 详细信息

目的探讨甘草泻心口腔护理液(甘草泻心口护液)改善脑卒中患者口腔状态的效果。方法采用随机对照试验,选取2021年12月至2023年10月在河南中医药大学第一附属医院神经外科治疗的80例脑卒中患者作为研究对象,以随机数表法分为对照组和观察组,各40例。对照组男25例、女15例,年龄(52.20±4.95)岁,病程(1.84±0.53)个月,予以生理盐水进行口腔护理,2次/d,20 ml/次,治疗10 d;观察组男26例、女14例,年龄(52.20±6.37)岁,病程(1.84±0.53)个月,采用甘草泻心口护液进行口腔护理,2次/d,50 ml/次,治疗10 d。对比两组患者治疗前后牙龈指数、菌斑指数、口腔pH值、口唇湿润度、口腔异味、口腔黏膜炎及不良反应发生情况。统计学方法采用t检验、χ^(2)检验。结果治疗前,两组患者牙龈指数、菌斑指数、口腔pH值、口唇湿润度、口腔异味发生情况比较,差异均无统计学意义(均P>0.05)。治疗5 d、10 d,观察组牙龈指数、菌斑指数、口唇湿润度及口腔异味情况均低于对照组[(1.23±0.42)分比(1.98±0.16)分、(0.65±0.48)分比(1.10±0.30)分、(1.44±0.50)分比(1.93±0.27)分、(0.78±0.48)分比(1.20±0.52)分、(0.65±0.53)分比(0.98±0.42)分、(0.15±0.36)分比(0.65±0.53)分、(2.28±0.45)分比(2.50±0.51)分、(1.45±0.60)分比(1.90±0.38)分],口腔pH值均高于对照组[(6.53±0.08)比(6.42±0.11)、(6.98±0.10)比(6.62±0.14)],差异均有统计学意义(t=10.506、4.987、5.416、3.814、3.086、4.936、2.096、4.007、5.115、13.234,均P<0.05)。治疗后,观察组口腔黏膜炎发生率为5.0%(2/40),低于对照组的15.0%(6/40),差异有统计学意义(χ^(2)=6.486,P<0.05)。两组患者治疗期间均无不良反应情况发生。结论甘草泻心口护液能有效改善脑卒中患者口腔状态,操作简便安全,患者依从性高,值得临床进一步推广应用。

关键词：脑卒中甘草泻心口腔护理液口腔状态

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Nickel-catalyzed arylcyanation of alkenes via cyano group translocation:access to 1,n-dinitriles or 4-amino nitriles

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Science China Chemistry 2024年第9期67卷 2975-2981页

作者： xiaohong Li Shenfan Wang Xiangxiang fu Donghui Xing Zeyuan fu Yuanfu Deng Huanfeng Jiang Liangbin Huang State Key Laboratory of Pulp and Paper Engineering School of Chemistry and Chemical EngineeringSouth China University of TechnologyGuangzhou 510640China

Herein,a nickel-catalyzed arylcyanation of unactivated alkenes via cyano group translocation with aryl boronic acids has been *** transformations provided a robust approach to constructing structurally diverse 1,n-dinitriles or 4-amino nitriles from easily prepared and commercially available starting *** cyano group translocation was achieved,involving the addition into the intramolecular C–N triple bond followed by the retro-Thorpe *** studies revealed that high temperature and CsHCO_(3) as the base were crucial for the cyano group translocation.

关键词： arylcyanation nickel catalysis cyano group translocation 1 n-dinitriles 4-amino nitriles

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晚期非小细胞肺癌患者营养不良-肌少症综合征患病率及影响因素分析

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成都医学院学报 2024年第4期19卷 710-713,718页

作者：余姣伏小红罗小珍吴驭雷莹谢灵灵四川大学华西医院老年医学中心/四川大学华西护理学院成都610041 四川大学华西医院急诊科/四川大学华西护理学院成都610041 成都上锦南府医院/四川大学华西医院上锦医院肿瘤科成都610041

目的分析晚期非小细胞肺癌(NSCLC)患者合并营养不良-肌少症综合征(MSS)的患病率,探讨其发生的相关影响因素。方法选取以2021年1—12月于四川大学华西医院上锦医院肿瘤科住院并接受抗肿瘤治疗的285例NSCLC患者作为研究对象,进行巢式病例... 详细信息

目的分析晚期非小细胞肺癌(NSCLC)患者合并营养不良-肌少症综合征(MSS)的患病率,探讨其发生的相关影响因素。方法选取以2021年1—12月于四川大学华西医院上锦医院肿瘤科住院并接受抗肿瘤治疗的285例NSCLC患者作为研究对象,进行巢式病例对照研究。同时存在营养不良和肌少症的患者判定为营养不良-肌少症综合征(MSS),纳入MSS组,未判定为MSS的患者纳入非MSS组。比较两组患者的年龄、婚姻状况、既往病史、血红蛋白、白蛋白、体重指数(BMI)等一般资料,并进行多因素分析,探讨MSS发生的相关影响因素。结果285例患者中有97例(34.04%)存在MSS,两组年龄、婚姻状况、BMI、血红蛋白、白蛋白、既往病史等一般资料比较,差异有统计学意义(P<0.05)。多因素分析结果显示,年龄≥70岁(OR=4.365,95%CI:2.227~8.554)、具有既往病史(OR=1.937,95%CI:1.037~3.617)、贫血(OR=0.981,95%CI:0.967~0.995)、低白蛋白血症(OR=0.911,95%CI:0.860~0.966)、BMI降低(OR=0.758,95%CI:0.686~0.838)均与NSCLC患者发生MSS相关(P<0.05)。结论高龄、有既往疾病史、贫血、低白蛋白血症、BMI降低均是NSCLC患者发生MSS的相关影响因素。MSS在NSCLC患者中患病率较高,亟需医护人员在日常照护中重视患者MSS的评估及早期干预。

关键词：非小细胞肺癌营养不良-肌少症综合征影响因素

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Characteristics and expression of the TCP transcription factors family in Allium senescens reveal its potential roles in drought stress responses

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BIOCELL 2023年第4期47卷 905-917页

作者： xiaohong fu JIE ZHAO DANDAN CAO CHENGXING HE ZIYI WANG YIBEI JIANG JIANFENG LIU GUIXIA LIU College of Life Science Institute of Life Science and Green DevelopmentHebei UniversityBaoding071000China Hebei Innovation Center for Bioengineering and Biotechnology Hebei UniversityBaoding071002China College of Plant Protection China Agricultural UniversityBeijing100193China

Allium senescens,is an important economic and ecological grassland plant with drought-resistant characteristics.A TCP protein transcription factor is important in the regulation of plant development and adverse ***,the mechanism by which TCP transcription functions in drought resistance in Allium senescens is still not ***,we obtained a total of 190,305 transcripts with 115,562 single gene clusters based on RNA-Seq sequencing of Allium senescens under drought *** total number of bases was 97,195,096 bp,and the average length was 841.06 ***,we found that there were eight genes of the TCP family that showed an upregulated expression trend under drought stress in Allium *** carried out an investigation to determine the evolution and function of the AsTCP family and how they produce an effect in drought *** 14 AsTCP genes were confirmed and divided into class I and class II containing CIN and CYC/TBI subfamilies,*** also found that the expression of AsTCP17 was remarkably upregulated with drought ***,the transformation of AsTCP17 in Arabidopsis revealed that the protective enzymes,namely polyphenol oxidase(POD)and superoxide dismutase(SOD),were increased by 0.4 and 0.8 times,*** content was also increased,while the H2O2 and malondialdehyde(MDA)contents were *** assays with 3,3′-diaminobenzidine(DAB)also suggested that the AsTCP17 downregulates reactive oxygen species(ROS)*** addition,overexpression of the AsTCP17 affected the accumulation of drought-related hormones in plants,and the synthesis of *** expression of AtSVP and AtNCED3,related ABA synthesis pathway genes,indicated that the level of expression of AtSVP and AtNCED3 was obviously enhanced,with the overexpression of line 6 showing a 20.6-fold and 7.0-fold increase,*** together,our findings systematically analyze the AsTCPs family at the transcriptome expression

关键词： Allium senescens Drought stress TCP transcription factor ABA synthesis pathway

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