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Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation

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Food Science and Human Wellness 2024年第1期13卷 421-433页

作者： Jingjing Liang Meina Zhang xiaohan Li yuan Yue Xiaowei Wang Mengzhen Han Tianli Yue Zhouli Wang Zhenpeng Gao College of Food Science and Engineering Northwest A&F UniversityYangling 712100China Xi'an GaoXin No.1 High School Xi'an 710065China

Plant-based fermentations provide an untapped source for novel biotechnological applications.In this study,a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes.Polysaccharides were extracted from fermented and non-fermented L.edodes and purified via DEAE-52 and Sephadex G-100.The components designated F-LEP-2a and NF-LEP-2a were analyzed by FT-IR,HPGPC,HPAEC,SEM,GC-MS and NMR.The results revealed that probiotic fermentation increased the molecular weight from 1.16×10^(4) Da to 1.87×10^(4) Da and altered the proportions of glucose,galactose and mannose,in which glucose increased from 45.94%to 48.16%.Methylation analysis and NMR spectra indicated that F-LEP-2a and NF-LEP-2a had similar linkage patterns.Furthermore,their immunomodulatory activities were evaluated with immunosuppressive mice.NF-LEP and F-LEP improved immune organ indices,immunoglobulin(Ig G and Ig M)and cytokines concentrations;restored the antioxidation capacity of liver;and maintained the balance of gut microbiota.F-LEP displayed better moderating effects on the spleen index,immunoglobulin,cytokines and the diversity of gut microbiota than NF-LEP(200,400 mg/kg).Our study provides an efficient and environment-friendly way for the structural modification of polysaccharides,which helps to enhance their biological activity and promote their wide application in food,medicine and other fields.

关键词： Lentinus edodes polysaccharide Lactobacillus fermentum fermentation Structural analysis Immunoregulatory activity Nuclear magnetic resonance

Functional nanoemulsions: Controllable low-energy nanoemulsification and advanced biomedical application

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Chinese Chemical Letters 2024年第2期35卷 57-69页

作者： Maojie Zhang Qiang Cao Yuming yuan xiaohan Guo Dawei Pan Rui Xie Xiaojie Ju Zhuang Liu Wei Wang Liangyin Chu College of Engineering Sichuan Normal UniversityChengdu 610101China School of Chemical Engineering Sichuan UniversityChengdu 610065China State Key Laboratory of Polymer Materials Engineering Sichuan UniversityChengdu 610065China

Nanoemulsions are widely used as advanced pharmaceutical delivery systems in biomedical field, due to their high encapsulation efficiency and good therapy efficacy.Nanoemulsification techniques that produce nanoemulsions with controllable sizes and compositions are promising for creating advanced nanoemulsion systems for pharmaceutical delivery.This review summarizes recent advances on low-energy emulsification techniques for producing nanoemulsions, and the use of these nanoemulsions as advanced pharmaceutical delivery systems and as templates to create drug-loaded functional particles for biomedical application.First, nanoemulsification techniques that utilize elaborate interfacial physics/chemistry and micro-/nano-fluidics, featured with relatively-low energy input, to produce nanoemulsions with controllable sizes and compositions, are introduced.Uses of these nanoemulsions to create nanoemulsionincorporated milli-particles, drug-loaded nanoparticles and nanoparticle-incorporated microparticles with sizes ranging from several millimeters to sub-10 nm are emphasized.Flexible and efficient use of the nanoemulsions, functional nanoparticles and milli-/micro-particles integrated with nanoemulsions or nanoparticles for advanced pharmaceutical delivery in biomedical field are highlighted, with focus on how the interplay between their sizes and compositions achieve desired pharmaceutical-delivery performances.Finally, perspectives on further advances on the controllable production of nanoemulsions are provided.

关键词： Nanoemulsions Interfaces Drug delivery Template synthesis Nanoparticles

HFIP-promoted catalyst-free cascade reactions for the synthesis of biologically relevant 3,3-di(indolyl)indolin-2-ones from indoles and isatins

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Chinese Chemical Letters 2020年第9期31卷 2465-2468页

作者： xiaohan yuan Shuai Wang Jialing Cheng Bin Yu Hong-Min Liu School of Pharmaceutical Sciences Zhengzhou UniversityZhengzhou 450001China Gordon Center for Medical Imaging Massachusetts General Hospital and Harvard Medical SchoolBostonMA 02129United States State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China

The first HFIP-promoted catalyst-free cascade reactions for the synthesis of biologically relevant 3,3-di(indolyl)indolin-2-ones(27 examples,up to 98% yield) from readily available indoles and isatin derivatives are described.This protocol shows well tolerance of different functional groups and features mild reaction conditions such as short reaction time(~1 h),no usage of catalyst,easy operation and product isolation.Of particular intere st is the formation of two C-C bonds and one all-carbon quaternary center.This protocol could serve as an alternative strategy to synthesize biologically important 3,3-di(indolyl)indolin-2-ones for biological testing.

关键词： Indoles Isatins HFIP Catalyst-free synthesis 3,3-Di(indoly)indolin-2-ones

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胃癌患者临床结局评估测量工具及应用进展

中华胃肠外科杂志 2024年第1期27卷 92-98页

作者：赵慧孙倩蒋晓涵袁秀红彭俊生中山大学护理学院广州510080 中山大学肿瘤防治中心胃外科广州510060 中山大学附属第六医院胃肠外科中山大学附属第六医院普通外科广州510655

胃癌作为一种常见的消化道肿瘤, 在全球范围内的发病及死亡情况仍不容乐观。尤其是进展期胃癌, 患者的生存结局是临床重要的关注点, 同样也是临床结局评估中重要的结局指标。本文通过对临床结局评估的定义及其在胃癌患者中可应用的测量... 详细信息

胃癌作为一种常见的消化道肿瘤, 在全球范围内的发病及死亡情况仍不容乐观。尤其是进展期胃癌, 患者的生存结局是临床重要的关注点, 同样也是临床结局评估中重要的结局指标。本文通过对临床结局评估的定义及其在胃癌患者中可应用的测量工具进行综述, 阐述临床结局评估工具的详细分类, 并对临床结局评估在胃癌中的应用现状进行综述, 分析其应用的效果及不足, 以期为临床工作人员选择合适的工具提供参考, 为推动精准医学的发展而进行全面、整体的临床结局评估提供帮助。

关键词：胃肿瘤临床结局评估患者报告结局观察者报告结局临床医生报告结局表现结局测量工具

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有氧水环境中藻类产生甲烷的研究进展

地球科学进展 2024年第2期39卷 157-168页

作者：于晓涵李哲肖艳袁翰卿李清中国科学院重庆绿色智能技术研究院中国科学院水库水环境重点实验室重庆400714 中国科学院大学重庆学院重庆400714 重庆交通大学河海学院重庆400074

自然水体是重要的甲烷排放来源,传统观点认为甲烷是微生物在严格厌氧条件下利用有机物的最终产物,但越来越多的证据表明有氧水环境中存在甲烷过饱和现象,被称为“甲烷悖论”。近年的研究表明,上述现象的产生与藻类的存在息息相关。藻类... 详细信息

自然水体是重要的甲烷排放来源,传统观点认为甲烷是微生物在严格厌氧条件下利用有机物的最终产物,但越来越多的证据表明有氧水环境中存在甲烷过饱和现象,被称为“甲烷悖论”。近年的研究表明,上述现象的产生与藻类的存在息息相关。藻类不仅可以通过光合作用或利用甲基化前体物质直接产生甲烷,也可为其他微生物提供厌氧微环境以及前体物质间接促进甲烷生成。然而,针对藻类有氧产甲烷的微生态机制方面的研究尚存不足,也使得全球甲烷收支核算仍存在较大不确定性。未来,进一步突破藻类有氧产甲烷的分子调控机理、揭示藻类对外部条件响应的有氧产甲烷特征及其生理适应性机制,将是深化领域研究的重要方向。

关键词：藻类有氧甲烷生成甲基化前体物质光合作用

Budd-Chiari综合征合并肝肺综合征1例并文献复习

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中华儿科杂志 2024年第1期62卷 71-75页

作者：田凤艳董萧侯晓寒袁如月潘元威张大郑州大学第一附属医院儿科郑州450052 郑州大学第一附属医院放射科郑州450052 郑州大学第一附属医院小儿外科郑州450052

目的总结Budd-Chiari综合征合并肝肺综合征(HPS)患儿的临床特征及预后。方法回顾性分析2016年12月郑州大学第一附属医院儿科收治的1例Budd-Chiari综合征合并HPS患儿的临床资料。以“Budd-Chiari综合征”“肝肺综合征”“Budd-Chiari syn... 详细信息

目的总结Budd-Chiari综合征合并肝肺综合征(HPS)患儿的临床特征及预后。方法回顾性分析2016年12月郑州大学第一附属医院儿科收治的1例Budd-Chiari综合征合并HPS患儿的临床资料。以“Budd-Chiari综合征”“肝肺综合征”“Budd-Chiari syndrome”“hepatopulmonary syndrome”为关键词分别在中国知网、万方数据库、中国生物医学文献数据库、PubMed数据库进行检索(建库至2023年7月),结合本例资料,总结Budd-Chiari综合征合并HPS患儿(<18岁)的临床特征、诊疗经过及预后。结果患儿,男,13岁,因“发绀、活动后胸闷6个月,皮肤黄染1周”入院。入院体格检查可见全身皮肤黏膜、巩膜轻度黄染,口唇、眼周、四肢末端发绀。辅助检查可见肝功能及动脉血气分析异常,总胆红素53μmol/L,直接胆红素14μmol/L,间接胆红素39μmol/L;氧分压54 mmHg(1 mmHg=0.133 kPa),二氧化碳分压31 mmHg,肺泡动脉血氧梯度57 mmHg;腹部CT静脉造影示Budd-Chiari综合征(肝静脉型)、肝硬化、门静脉高压;增强经胸超声心动图造影(CE-TTE)阳性。患儿经对症支持治疗后院外氧疗,随访至2023年3月,低氧血症无明显改善,日常活动受限。文献复习符合检索条件中文文献0篇,英文文献3篇,结合本例患儿共4例,3例首诊原因为呼吸困难、发绀或低氧血症,1例不详。2例因呼吸系统症状就诊诊断Budd-Chiari综合征合并HPS,2例HPS分别发生于Budd-Chiari综合征确诊1.5和8.0年。CE-TTE阳性2例,肺灌注显像阳性2例。2例患儿行肝移植,呼吸功能恢复良好;吸氧治疗1例,低氧血症无改善;等待肝移植1例。结论Budd-Chiari综合征合并HPS早期起病隐匿,临床表现以呼吸困难、发绀多见,早期行CE-TTE明确肺内血管扩张可减少误诊。肝移植有助于改善预后。

关键词：儿童 Budd-Chiari综合征肝肺综合征

Discovery of [1,2,4]triazolo[1,5-a]pyrimidine derivatives as new bromodomain-containing protein 4(BRD4) inhibitors

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Chinese Chemical Letters 2020年第2期31卷 418-422页

作者： Shuai Wang Dandan Shen Lijie Zhao xiaohan yuan Jialing Cheng Bin Yu Yichao Zheng Hongmin Liu Key Laboratory of Technology of Drug Preparation Ministry of Education of China&School of Pharmaceutical SciencesZhengzhou UniversityZhengzhou 450001China

Targeting bromodomain-containing protein 4(BRD4) has been proved to be an effective strategy for cancer therapy.To date,numerous BRD4 inhibitors and degraders have been identified,some of which have advanced into clinical trials.In this work,a focused library of new [1,2,4]triazolo [1,5-a]pyrimidine derivatives were discovered to be able to inhibit BRD4.WS-722 inactivated BRD4(BD1/BD2),BRD2(BD1/BD2) and BRD3(BD1/BD2) broadly with the IC_(50) values less than 5 μmol/L.Besides,WS-722 inhibited growth of THP-1 cells with an IC_(50) value of 3.86 μmol/L.Like(+)-JQ1,WS-722 inhibited BRD4 in a reversible manner and enhanced protein stability.Docking studies showed that WS-722 occupied the central acetyl-lysine(Kac) binding cavity and formed a hydrogen bond with Asn140.In THP-1 cells,WS-722 showed target engagement to BRD4.Cellular effects of WS-722 on THP-1 cells were also examined,showing that WS-722 could block c-MYC expression,induce G0/G1 phase arrest and p21 up-regulation,and promote differentiation of THP-1 cells.BRD4 inhibition by WS-722 resulted in cell apoptosis and upregulated expression of cleaved caspased-3/7 and PARP in THP-1 cell lines.The [1,2,4]triazolo[1,5-a]pyrimidine is a new template for the development of new BRD4 inhibitors.

关键词： Epigenetic regulation BRD4 inhibitor triazolo[1,5-a]pyrimidine THP-1 cells AML treatment

不同培养温度下杉木叶凋落物添加对土壤CO_(2)释放及激发效应的影响

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生态学杂志 2024年第4期43卷 993-999页

作者：元方慧应宇馨陈子亮娄肖寒胡亚林福建农林大学林学院森林生态稳定同位素研究中心福州350002

凋落物输入能够影响土壤有机碳(SOC)输入与输出动态平衡。然而,针对气候变暖背景下凋落物输入对SOC矿化激发效应及温度敏感性的影响仍不清楚。本研究以^(13)C标记的杉木叶凋落物为对象,比较不同培养温度(15、25和35℃)条件下凋落物添加... 详细信息

凋落物输入能够影响土壤有机碳(SOC)输入与输出动态平衡。然而,针对气候变暖背景下凋落物输入对SOC矿化激发效应及温度敏感性的影响仍不清楚。本研究以^(13)C标记的杉木叶凋落物为对象,比较不同培养温度(15、25和35℃)条件下凋落物添加对不同来源CO_(2)释放速率和累积量、SOC矿化激发效应以及温度敏感性(Q_(10))的影响。结果发现:凋落物添加提高土壤总CO_(2)释放速率和累积量,但随着温度的升高,土壤总CO_(2)释放速率和累积量增加幅度呈下降趋势,且不同温度下土壤CO_(2)释放速率随培养时间的延长均呈降低趋势。与对照相比,凋落物添加促进土壤来源CO_(2)释放累积量,呈正激发效应。随着培养温度升高,添加凋落物处理的土壤来源和凋落物来源CO_(2)释放累积量均呈增加趋势,但SOC激发效应呈下降趋势。此外,凋落物添加降低土壤呼吸CO_(2)释放速率的温度敏感性,且凋落物来源CO_(2)释放速率的Q_(10)值低于土壤来源CO_(2)的Q_(10)值。本研究表明,不同培养温度下凋落物输入对土壤CO_(2)释放的影响存在差异,且凋落物输入能够降低土壤呼吸CO_(2)释放速率的温度敏感性。

关键词：叶凋落物土壤呼吸速率激发效应温度敏感性

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电弧增材制造薄壁件形状控制研究进展

材料导报 2024年第6期38卷 191-204页

作者：邱贺方侯笑晗郭晓辉崔帆帆侯根良张泽罗伟蓬袁晓静火箭军工程大学作战保障学院西安710000 第61363部队西安710000

电弧增材制造(WAAM)具有沉积速率快、材料利用率高等优势,在航空航天、军工生产、智能汽车、核电等领域具有广阔的应用前景和发展空间。然而,WAAM自身工艺特点的局限性使其难以充分发挥自由制造的优势。尤其是在制造复杂结构零件过程中,... 详细信息

电弧增材制造(WAAM)具有沉积速率快、材料利用率高等优势,在航空航天、军工生产、智能汽车、核电等领域具有广阔的应用前景和发展空间。然而,WAAM自身工艺特点的局限性使其难以充分发挥自由制造的优势。尤其是在制造复杂结构零件过程中,WAAM存在热积累和热循环产生残余应力和热变形等固有工艺性问题,而且复杂结构零件的结构特征也对WAAM控形精度带来不小挑战。本文从薄壁零件的结构特征出发,综述了WAAM直壁结构、尖角结构、交叉结构、倾斜/悬空结构和空间交叉结构薄壁件形状控制面临的难题,并对WAAM复杂结构薄壁件的形状控制研究进行了展望。

关键词：电弧增材制造控形精度路径规划薄壁结构