检索结果-南通市图书馆

Chinese Chemical Letters 2022年第6期33卷 3043-3048页

作者： Lida Ren Zhihao Ming Wei Zhang Yangwei Liao xiaofeng tang Bei Yan Huimin Lv Xianjin Xiao Centre of Reproductive Medicine/Family Planning Research Institute Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Obstetrics and Gynecology Third Hospital of Shanxi Medical University.Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalTongji Medical College.Huazhong University of Science and TechnologyTaiyuan 030032China

Enzyme assisted DNA probes are powerful tools in molecular diagnostics for their simplicity,rapidity,and low detection limit.However,cost of probes,difficulty in optimization and disturbance of secondary structure hindered the wider application of enzyme assisted DNA probes.To solve the problems,we designed a new system named shared-probe system.By introducing two unlabeled single stranded DNA named Sh1 and Sh2 as the bridge between probe and the substrate,the same sequence of dually labeled probe with stable performance was shared for different mutations,thus sparing the expense and time cost on designing,synthesizing and optimizing corresponding probes.Besides,the hybridization between Sh1 and the substrate could overcome secondary structures,which guaranteed the detection of different substrates.The performance and generality of the design were tested by low abundance detection in synthetic single DNA samples and the limit of detection was 0.05%for PTENR130 Q,EGFR-L858 R and 0.02%for BRCA1-NM007294.3.In genomic DNA samples,the limit of detection of 0.1%can be achieved for EGFR-L858 R,demonstrating the potential of clinical application in our design.

关键词： Nucleic acid probe EndonucleaseⅣ Secondary structure Genomic mutation SNP detection

Thermodynamics-guided two-way interlocking DNA cascade system for universal multiplexed mutation detection

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2022年第1期33卷 334-338页

作者： Wei Zhang Liquan Liu Yangwei Liao Wan Shu xiaofeng tang Kejun Dong Zhihao Ming Xianjin Xiao Hongbo Wang Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Detection of point mutations in driver genes is of great significance for the early diagnosis,treatment,and prognostic evaluation of cancer.However,current detection methods do not offer versatility,specificity,and rapid performance simultaneously.Thus,multiple mutation detection processes are necessary,which results in long processing times and high costs.In this study,we developed a thermodynamics-guided two-way interlocking DNA cascade system for universal multiplexed mutation detection(TTI-CS).This strategy is based on the DNA probe,which changes the thermodynamic balance of the DNA cascade by the designed bubble structure,thereby achieving a good distinction between mutant and wild-type DNA.The designed method greatly shortens the detection time through two-way intrusion.In addition,this method only changes two inexpensive trigger and bridge sequences,which replace the specific and expensive nucleic acid probes used in analyses based on traditional DNA probe methods,thereby enabling multiple detections.We performed the detection of synthetic single-stranded DNA for the five mutation points and successfully detected in endometrial cancer specimens.The detection limit of this method is0.1%,which better meets the needs of clinical low-abundance multiple mutation detection.Overall,TTI-CS is currently one of the best methods for detecting multiple mutation detections.

关键词： Nucleic acid probes DNA cascade system Universality Low-abundance Multiple mutation detection

A new method for evaluating the quality of single sperm by detecting reactive oxygen species

在线全文

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2023年第2期34卷 306-310页

作者： Hao Hu Bei Yan Wei Zhang Wenbo Yan Liquan Liu xiaofeng tang Kejun Dong Longjie Li Xianjin Xiao Chengliang Xiong Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Sperm Bank General Hospital of Ningxia Medical UniversityYinchuan 750004China Department of Obstetrics and Gynecology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China School of Life Science and Technology Wuhan Polytechnic UniversityWuhan 430023China Reproductive Medicine Center Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Hubei Province Human Sperm Bank Wuhan 430030China

Sperm damage caused by reactive oxygen species(ROS) is one of the main causes of male infertility.Therefore, the level of ROS in sperm is an important indicator for the diagnosis and prognosis of male infertility. Herein, we constructed a single sperm ROS detection method(SSRDM) with an optical microprobe fabricated via focused ion beam process. The micro-probe is used to separately excite fluorescence in the sperm and the area around the sperm after ROS staining, and the difference in fluorescence values can reflect the level of ROS in the sperm. We collected 102 semen samples and 72 of them were divided into asthenozoospermia and non-asthenozoospermia groups. SSRDM and flow cytometry were used to detect the ROS levels of the two groups. The results of SSRDM showed that the ROS levels of asthenozoospermia group were higher than that of non-asthenozoospermia group(P = 0.002), while the results of flow cytometry indicated no difference(P = 0.152). In terms of ROS levels, compared with flow cytometry, SSRDM has a stronger ability to distinguish between those two groups, providing a reliable basis for assessment of sperm quality. Another 30 semen samples were used to investigate temperature and temporal variability of SSRDM to ensure the stability and accuracy of this method. Overall, we have developed a method that can quantitatively detect fluorescent substances in sperm at the single-cell level supplying evidence for diagnosis and prognosis of male infertility.

关键词： Reactive oxygen species Single sperm Male infertility Microprobe Flow cytometry

Non-destructive Measurement of Magnetic Properties of Claw Pole

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Mechanical Engineering 2019年第5期32卷 23-33页

作者： Chengliang Hu Xuejiao Bai Minjun tang xiaofeng tang Zhen Zhao Institute of Forming Technology&Equipment School of Material Science and EngineeringShanghai Jiao Tong UniversityShanghai 200030China Jiangsu Longcheng Precision Forging Co. Ltd.Jiangsu 213100China

The magnetic properties of the claw pole have a direct effect on the output power of a generator Many methods can be used to measure these magnetic properties,each with its own advantages,but an important shortcoming is that all are destructive.In this study,a new non-destructive method to measure the magnetic properties of claw pole was proposed and a corresponding testing set-up was designed.A finite-element model was constructed to simulate the measurement process.Results proved that the measured magnetization-like curves had good agreement with the trend of the input magnetic curves and the effect of the positioning error in the measuring process could be neglected.To further validate the new method,seven types of claw poles of different materials subjected to different heat-treatment processes were forged and tested by both the new method and the conventional ring-sample method.Compared with the latter,the new method showed better consistency,relatively higher accuracy,and much stronger stability of measurement results;however,its sensitivity needs to be improved.The effects of material compositions and heat-treatment parameters on the magnetic properties of the claw pole were briefly analyzed.

关键词： Claw pole Magnetic property Measurement Ring sample Accuracy Stability

在线全文

学校读者我要写书评

暂无评论

新手工程师角色转换期的职业韧性塑造机制

高等工程教育研究 2024年第1期 78-85页

作者：马立超唐潇风清华大学教育研究院清华大学教育研究院工程教育研究所

工程场域固有的复杂性、艰难性和未知性对工程师的韧性提出较高挑战,对新手工程师而言,职业韧性在从校园迈向职场的角色转换过程中发挥重要作用。对16名新手工程师进行深度访谈,通过扎根编码构建出新手工程师职业韧性塑造的CRA理论模型... 详细信息

工程场域固有的复杂性、艰难性和未知性对工程师的韧性提出较高挑战,对新手工程师而言,职业韧性在从校园迈向职场的角色转换过程中发挥重要作用。对16名新手工程师进行深度访谈,通过扎根编码构建出新手工程师职业韧性塑造的CRA理论模型。研究发现:危机逆境(Perceived Mini-Crises)、支持要素(Facilitative Resources)、适应行为(Positive Adaptation)是新手工程师职业韧性的三大支柱。新手工程师职业韧性形成的危机逆境表现为工程师与工作、人际、外界和自我的冲突,涵盖工作特征、关系网络、外部环境、自我挣扎等互动情境;支持要素包括外驱条件、内生特质和理想信念三类;适应行为分为依从抗压型和变革突破型。其中,危机逆境是职业韧性的触发因素,支持要素是韧性形成的助力条件,适应行为是良好韧性的外显形态。上述结论有助于延展韧性的概念谱系和理论框架,同时还启发高校应充分重视通识教育对工程人才培养的价值,并将韧性纳入工程教育培养目标体系,工程实践界也要为工程师职业发展创造良好的外部环境和健全的支持体系。

关键词：新手工程师职业韧性角色转换职业适应扎根理论

改良牙根屏障技术即刻种植即刻修复后口内美学区软组织轮廓动态监测及美学评估

在线全文

学校读者我要写书评

暂无评论

中华口腔医学杂志 2024年第6期59卷 551-558页

作者：唐晓枫吴庆庆陈曦王媛付钢重庆医科大学附属口腔医院种植科口腔疾病与生物医学重庆市重点实验室重庆市高校市级口腔生物医学工程重点实验室重庆401147

目的探讨改良牙根屏障技术(MSST)即刻种植即刻修复后1年内种植位点唇侧软组织的动态变化,为评估MSST对种植体周软组织的作用提供依据。方法前瞻性纳入2022年1月至2024年1月于重庆医科大学附属口腔医院种植科完成MSST美学区即刻种植即刻... 详细信息

目的探讨改良牙根屏障技术(MSST)即刻种植即刻修复后1年内种植位点唇侧软组织的动态变化,为评估MSST对种植体周软组织的作用提供依据。方法前瞻性纳入2022年1月至2024年1月于重庆医科大学附属口腔医院种植科完成MSST美学区即刻种植即刻修复术后1年的患者,分别于术前和术后3、6、12个月使用口内扫描仪获取患者口内光学模型,导入Geomagic Studio 2013软件进行多时间点虚拟轮廓重叠配准,于多个层面分析种植位点唇侧软组织轮廓变化,精确计算术后各时间点唇侧龈缘顶点冠根向变化量、龈乳头冠根向变化量及唇侧软组织轮廓唇腭向厚度平均变化量,并通过粉色美学评分进行美学效果评价。结果共纳入患者22例(22枚种植体),患者年龄为(40±13)岁(21~75岁),包括9例男性和13例女性,12个月的随访观察期内,所有种植体均无不良事件发生。术后12个月种植位点唇侧龈缘顶点冠根向变化量为0.08(0.07)mm,近中龈乳头冠根向变化量为0.19(0.25)mm,远中龈乳头冠根向变化量为0.19(0.10)mm,唇侧软组织轮廓唇腭向厚度平均变化量为(0.39±0.09)mm,轮廓塌陷主要发生于龈缘根方2 mm内。种植位点唇侧牙槽骨高度变化量为(0.17±0.08)mm,种植体肩台根方1、3、5 mm处水平向唇侧牙槽骨厚度变化量分别为(0.13±0.08)、(0.12±0.10)、0.04(0.17)mm。粉色美学评分为13.00(2.25)分,所有种植位点均获得理想的美学效果。结论利用MSST于美学区行即刻种植即刻修复,12个月随访观察期内,种植位点唇侧软组织轮廓变化微量。

关键词：美学,牙科牙种植即刻种植即刻修复牙根屏障技术种植体周软组织轮廓

在线全文

学校读者我要写书评

暂无评论

辐射加热颗粒槽道湍流两相流的相间传热特性

Acta Mechanica Sinica 2024年第2期40卷 54-64页

作者：唐晓峰潘明杨文武董宇红 Shanghai Institute of Applied Mathematics and Mechanics School of Mechanics and Engineering ScienceShanghai UniversityShanghai200072China Shanghai Key Laboratory of Mechanics in Energy Engineering and Shanghai Institute of Aircraft Mechanics and Control Shanghai200072China

本文采用直接数值模拟方法结合拉格朗日点-粒模型研究了携带辐射热惯性颗粒的竖直槽道湍流两相流相间传热特性.研究中采用双向耦合模型描述分散相颗粒与流体之间的动量交换和热交换,辐射强度和颗粒Stokes数范围分别为1≤q/q0≤20,0.36×... 详细信息

本文采用直接数值模拟方法结合拉格朗日点-粒模型研究了携带辐射热惯性颗粒的竖直槽道湍流两相流相间传热特性.研究中采用双向耦合模型描述分散相颗粒与流体之间的动量交换和热交换,辐射强度和颗粒Stokes数范围分别为1≤q/q0≤20,0.36×10^(−1)≤Stf≤2.16×10^(−1).研究发现辐射加热颗粒对流体的动力学调制加速了流体运动,使湍流强度增大.高辐射下的小Stokes数颗粒能显著加热流体.我们从流场时均温度方程出发,导出了两相流系统中相间传热Nusselt数的理论表达式,理论分析表明相间传热引起冷板和热板Nusselt数的差异,并进一步得到了相间传热效率与颗粒Stokes数及颗粒所受辐射强度的标度关系.

关键词：加热流体 Stokes数传热效率辐射加热辐射强度直接数值模拟拉格朗日点理论表达式

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

新质人才培养:高校微生物学教学的新航标

微生物学通报 2024年第4期51卷 1051-1054页

作者：唐晓峰陈向东武汉大学生命科学学院湖北武汉430072

习近平总书记提出的“新质生产力”是以科技创新为驱动,以高层次创新型人才为支撑,以战略性新兴产业和未来产业为载体,以数字化、智能化、绿色化为基石的高质量生产力。培养一大批掌握现代技术、具有专业知识的创新型新质人才无疑是新... 详细信息

习近平总书记提出的“新质生产力”是以科技创新为驱动,以高层次创新型人才为支撑,以战略性新兴产业和未来产业为载体,以数字化、智能化、绿色化为基石的高质量生产力。培养一大批掌握现代技术、具有专业知识的创新型新质人才无疑是新时代背景下培育和发展新质生产力的最根本基石,是高校教育教学改革发展的新航标和新机遇。以“第十九届全国微生物学教学和科研及成果产业化研讨会”为契机,《微生物学通报》邀稿组织出版了本期“高等院校教学主题刊”,较全面地反映了近两年我国微生物学教学改革与人才培养的最新进展和发展态势。可以看到,教师的教学模式、学生的学习方式、师生的交流方式等与疫情前相比都发生了巨大的变化,现代教育信息化技术和新媒体平台的运用已经常态化。教学理念的转变、教学资源的整合等也都随之发生了变化。线上线下结合、翻转课堂等教学模式已成为高校的日常,重视对学生创新意识、探索精神和实践能力的培养已成为共识。期望该主题刊的出版有助于进一步促进广大微生物学教师适应国家提出的“加快发展新质生产力,积极打造生物制造新引擎”的需求,推动微生物学教学持续改革迈上新台阶,最终达到提高人才培养质量和加强拔尖创新人才自主培养的目的。

关键词：微生物学课程教学改革创新意识实践能力新质生产力

Short-DNA Specific Blocker PCR for Efficient and Simple Enrichment of Cell Free Fetal DNAs with Short Lengths

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2021年第8期39卷 2101-2106页

作者： Yangwei Liao xiaofeng tang Zhihao Ming Lida Ren Wei Zhang Xianjin Xiao Institute of Reproductive Health Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China NHC Key Laboratory of Birth Defect for Research and Prevention(Hunan Provincial Maternal and Child Health Care Hospital) ChangshaHunan 410008China

As an inn ate characteristic,DNA length has attracted more and more atte ntion.The length of some import ant biomarkers such as ctDNA(Circulating tumor DNA)and cffDNA(Cell free fetal DNA)is shorter than that of cell-free DNA.Researchers have utilized the differe nee in length to in crease the abunda nee of ctDNA or cffDNA in total cell-free DNA to overcome the difficulties in detecti on due to their low abundance.

关键词： DNA Enrichment Polymerase chain reaction Gene seque ncing Non-invasive prenatal testing

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

热成形条件下软磁材料的磁感应强度预测模型

机械工程学报 2024年第2期60卷 132-139,149页

作者：胡成亮苗宏量曾凡赵震汤敏俊汤晓峰上海交通大学模具CAD国家工程研究中心上海200030 上海交通大学塑性成形技术与装备研究院上海200030 江苏龙城精锻有限公司常州213100

爪极是车用爪极式发电机中的重要零件之一,其磁性能直接影响对应发电机的发电效率。热锻是当前工业界爪极制造的常用工艺,面向爪极热锻成形本文开展了爪极锻件磁性能的预测研究工作。以爪极用低碳钢软磁材料为对象,构建热成形条件下的... 详细信息

爪极是车用爪极式发电机中的重要零件之一,其磁性能直接影响对应发电机的发电效率。热锻是当前工业界爪极制造的常用工艺,面向爪极热锻成形本文开展了爪极锻件磁性能的预测研究工作。以爪极用低碳钢软磁材料为对象,构建热成形条件下的磁感应强度预测模型,并介绍了该模型的主要参数及其确定方法;对爪极热锻过程开展数值模拟,引入磁感应强度预测模型试着对爪极锻件磁感应强度分布的进行预测;同时,在爪极预锻不同变形阶段的中心部位及终锻件不同位置进行取样,采用磁环法对具体磁感应强度进行实测,与模拟计算结果进行对比分析;模拟预测与实际测量值间最大误差小于10%,说明该预测模型具有良好的适用性,可为后续面向磁性能提升的爪极工艺设计优化奠定基础。

关键词：爪极磁感应强度热成形预测模型数值模拟