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Effectiveness of escape vent shape in crab pots for releasing swimming crab Portunus trituberculatus in the East China sea

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Aquaculture and Fisheries 2023年第3期8卷 332-340页

作者： Jian Zhang Xiaofei Shi Pingguo He Jiangao shi College of Marine Sciences Shanghai Ocean UniversityShanghai201306China National Engineering Research Centre for Oceanic Fisheries Shanghai Ocean UniversityShanghai201306China Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China School for Marine Science and Technology University of Massachusetts DartmouthNew BedfordMA02744USA East China Sea Fisheries Research Institute Chinese Academy of Fishery SciencesShanghai200090China

To stop the decline of swimming crab(Portunus trituberculatus)stocks in the East China Sea,escape vents on crab pots are urgently required to improve pot selectivity and enable release of sublegal-sized crabs.This study presents field comparative fishing experiments,which examined the effectiveness of different shapes and sizes of escape vents in crab pots for the release of swimming crabs.Two sizes each of rectangular,elliptic,and circular escape vents were tested.The results of comparative fishing experiments indicated that experimental pots with escape vents,regardless of their configuration,caught significantly fewer sublegal-sized crabs(<49 mm carapace length,or<116 mm carapace width)than unmodified control pots.Although legal-sized swimming crabs were also less caught,analysis of size selectivity showed that rectangular vents produced steeper selectivity curves than elliptic and circular escape vents.This indicates that rectangular vents may be more suitable and controllable when escape vents will become a requirement and their use will be enforced in fisheries.Underwater observations were conducted using GoPro cameras and LED lights during the first 2.5 h of the deployments(which usually lasted between 5 and 12 h).Video recordings of experimental pots equipped with rectangular or elliptic escape vents showed that swimming crabs trapped in the pots could detect the vents,and readily approached and passed through these.However,no significant difference was detected in the response behavior of swimming crabs between rectangular and elliptic vent pots when quantitative indicators,derived from video recordings,were compared.The effect of different vent shapes on the escape behavior of swimming crab gradually manifested as an increased soak time and by the onset of the escape of larger crabs.Future underwater observations should thus last the entire duration of pot soaking to study how crabs escape from the pots.

关键词： Crab pots Portunus trituberculatus Escape vents Vent shape Size selection Underwater observation

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Multileveled Engagement:A Cognitive Empirical Approach to Crossover Picturebooks

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Language and Semiotic Studies 2020年第4期6卷 25-45页

作者： Xiaofei Shi Soochow University China

Crossover picturebooks attract readers of different ages.The trend of refocusing on the body in children’s literature criticism,especially under the influence of literary cognitive studies as Maria Nikolajeva points out(2016),provides a context to reconfigure our understanding of crossover picturebooks.Informed by literary cognitive studies,particularly the theory of brain laterality,the project selects the picturebook that may have crossover potential to start with,then investigates the actual readers’engagement with the potentially crossover picturebook,and finally feeds the insights gleaned from the empirical study back into the understanding of the selected text,and possibly crossover picturebooks generally.The project aims to learn about the crossover picturebook through exploring how it impacts readers.It was a small-scale case study,conducted in the east of England,UK.Colin Thompson’s How to Live Forever was used with two adults and two children.Observation and interviewing were used to collect data.Text-related creative activities served to support or clarify the oral response.The project reveals:the multileveled nature of crossover picturebooks that many scholars emphasize may correspond to the multileveled engagement that texts can elicit from readers,which is partially the outcome of cognitive-affective skills and mechanisms engaged differently across children and adults.This study also shows the crossover potential particular to picturebooks:picturebooks may use the visual to flesh out the embodied experience,which can springboard further thinking about abstract,complicated notions.This mode of representation corresponds to the way the human mind works,thus engaging children and adults alike.

关键词： crossover picturebook cognitive literary studies empirical research

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Crossover Literature: Transcending Cognitive-Affective Boundaries——Exemplified with a Close Analysis of Anthony Browne’s The Big Baby

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Language and Semiotic Studies 2019年第2期5卷 101-118页

作者： Xiaofei Shi Soochow University

This article explores crossover literature, that is, literature transcending age boundaries and, in particular, the boundaries between adult and child readership. This article suggests that texts transcending age boundaries are not a recent phenomenon—adults have almost always existed in children’s literature as a background authority or a hidden and even active addressee. But it is the changes in a social, historical, and cultural context that call for a new term, crossover, to designate this phenomenon. Central to the discussion of crossover literature is how to conceptualise the different readings that a text can elicit from child and adult readers. Drawing on developments in cognitive literary studies, this article argues that crossover literature is characterised by its potential to accommodate both child and adult readers’ different levels of cognitive-affective development, subject to the factor of age. The argument is exemplified with a close analysis of Anthony Browne’s picturebook The Big Baby(1995). It is particularly productive and illuminating to discuss how crossover picturebooks can attract both children and adults, contrary to the traditional assumption that picturebooks are merely 'kids’ stuff.' The close analysis of The Big Baby reveals that cognitive literary studies can tease out the implications of the age difference, which are crucial to crossover research, and shed new light on how crossover literature may attract readers of different ages.

关键词： crossover literature crossover picturebook cognitive literary studies Anthony Browne

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Growth structures and growth strata of the Qianjiadian Basin in the western Yanshan fold and thrust belt, North China

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Science China Earth Sciences 2019年第7期62卷 1092-1109页

作者： Xiaofei Shi Shaofeng LIU Chengfa LIN State Key Laboratory of Geological Processes and Mineral Resources and School of Geosciences and Resources China University of Geosciences (Beijing)

The northwestward subduction of the Izanagi Plate beneath the eastern Eurasian Plate during the Late Mesozoic caused a series of compressional deformation events in the Yanshan fold and thrust belt(YFTB), but the tectonic deformation timing, tectonic properties and relationship between tectonic uplift and sediment accumulation in the intermontane basins continue to be debated. For this reason, the sedimentology, sediment provenance, and basin structure of the Qianjiadian Basin(QJB) in the northern Beijing region during the Late Jurassic and Early Cretaceous were studied in detail. The results suggest that a fault-propagation fold-type(FPFT) growth structure and growth strata developed on the western edge of the QJB and that the top part of Member 2 and Member 3 of the Tuchengzi Formation are growth strata controlled by the limb rotation mechanism. In two small thrust faults in the QJB, the Mesoproterozoic Xiamaling Formation is thrust over the Tuchengzi Formation, and these faults may have controlled the development of the fault-bend fold-type growth strata. An analysis of the "source-to-sink" process suggests that the hanging wall succession of the Shangyi-Pingquan fault(SPF) was the main source area of the Late Jurassic to early Early Cretaceous strata in the QJB. A zircon206 Pb/238 U age of 140.8±2.4 Ma for the volcanic rocks at the bottom of the FPFT growth strata represents the timing of the initiation of FPFT growth structure development. The discovery of the FPFT growth structure and growth strata in the QJB indicate that the QJB was an intermontane flexural basin controlled by fold and thrust structures during the early Early Cretaceous. Near the E-W-trending SPF, the NE-SW-trending Qianjiadian thrust fault(QJTF) and two small intrabasinal thrust faults may constitute a unified right-lateral strike-slip system that formed in response to the northwestward flat subduction of the Izanagi Plate beneath the East Asian continent during the Jurassic-Early Cretaceous.

关键词： Yanshan fold and thrust belt Qianjiadian Basin Tuchengzi Formation Growth structure Growth strata Izanagi Plate

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Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses

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Genomics, Proteomics & Bioinformatics 2021年第5期19卷 707-726页

作者： Ruona shi Zhenhuan Feng xiaofei Zhang CAS Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineCenter for Cell Lineage and DevelopmentGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou 510530China University of Chinese Academy of Sciences Beijing 100049China Center for Cell Lineage and Atlas Bioland LaboratoryGuangzhou Regenerative Medicine and Health Guangdong LaboratoryGuangzhou 510530China

The coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection is currently a global pandemic.Extensive investigations have been performed to study the clinical and cellular effects of SARS-CoV-2 infection.Mass spectrometry-based proteomics studies have revealed the cellular changes due to the infection and identified a plethora of interactors for all SARS-CoV-2 components,except for the longest non-structural protein 3(NSP3).Here,we expressed the full-length NSP3 proteins of SARS-CoV and SARS-CoV-2 to investigate their unique and shared functions using multi-omics methods.We conducted interactome,phosphoproteome,ubiquitylome,transcriptome,and proteome analyses of NSP3-expressing cells.We found that NSP3 plays essential roles in cellular functions such as RNA metabolism and immune response(e.g.,NF-κB signal transduction).Interestingly,we showed that SARS-CoV-2 NSP3 has both endoplasmic reticulum and mitochondrial localizations.In addition,SARS-CoV-2 NSP3 is more closely related to mitochondrial ribosomal proteins,whereas SARS-CoV NSP3 is related to the cytosolic ribosomal proteins.In summary,our integrative multi-omics study of NSP3 improves the understanding of the functions of NSP3 and offers potential targets for the development of anti-SARS strategies.

关键词： NSP3 SARS-CoV-2 SARS-CoV Multi-omics Mitochondria

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Single-cell transcriptomic landscape of peripheral blood cells provides insights into adaptation of red-eared sliders(Trachemys scripta elegans)

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Integrative Zoology 2024年第3期19卷 468-479页

作者： Rui GUO Guangwei MA xiaofei ZHAI Haitao shi Jichao WANG Ministry of Education Key Laboratory for Ecology of Tropical Islands Key Laboratory of Tropical Animal and Plant Ecology of Hainan ProvinceCollege of Life SciencesHainan Normal UniversityHaikouChina

Red-eared sliders(Trachemys scripta elegans),as one of the 100 most threatening aliens,have stronger immunity than the native species in response to environmental stress.Blood cells are an important component of immunity in the body.However,the blood cell researches of turtle are still in the traditional blood cell classification and morphological structure observation.Furthermore,turtle granulocytes cannot be accurately identified using traditional methods.Single-cell RNA sequencing techniques have been successfully implemented to study cells based on the mRNA expression patterns of each cell.The present study profiled the transcriptomes of peripheral blood cells in red-eared sliders to construct a single-cell transcriptional landscape of the different cell types and explored environmental adaptation mechanism from the perspective of hematology.All 14 transcriptionally distinct clusters(platelets,erythrocytes1,erythrocytes2,CSF1R monocytes,POF1B monocytes,neutrophils,GATA2high basophils,GATA2low basophils,CD4 T cells,CD7 T cells,B cells,ACKR4 cells,serotriflin cells,and ficolin cells)were identified in the peripheral blood cells of the red-eared sliders.In particular,a subtype of erythrocytes(erythrocytes1)that expressed immune signals was identified.Peripheral blood cells were grouped into three lineages:platelet,erythroid/lymphoid,and myeloid cell lineages.Furthermore,based on differentiation trajectory and up-regulated gene expression,ACKR4 cells were newly identified as lymphocytes,and serotriflin and ficolin cells as granulocytes.The single-cell transcriptional atlas of the peripheral blood cells in red-eared sliders provided in the present study will offer a comprehensive transcriptome reference for the exploration of physiological and pathological hematology in this species.

关键词： blood cells single-cell RNA-seq Trachemys scripta elegans

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Synergism of 1D CdS/2D Modified Ti_(3)C_(2)T_(x) MXene Heterojunctions for Boosted Photocatalytic Hydrogen Production

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Chinese Journal of Structural Chemistry 2022年第8期41卷 58-64页

作者： shi Cheng Qianqian Xiong Chengxiao Zhao xiaofei Yang College of Science Nanjing Forestry UniversityNanjing 210037China

Rational design and controllable synthesis of visible-light-responsive photocatalysts that exhibit both good hydrogen-producing efficiency and stability in the water splitting reaction are undoubtedly a challenge. Here we report an integrated CdS nanorod/oxygen-terminated Ti_(3)C_(2)T_(x) MXene nanosheet heterojunction with a high catalytic hydrogen evolution reaction(HER) activity. By incorporating one-dimensional(1D)CdS nanorods onto annealed ultrathin two-dimensional(2D) MXene nanosheets, the mixed-dimensional 1D/2D heterojunction achieved a hydrogen-evolving rate of 8.87 mmol·g^(-1)·h^(-1), much higher than that of bulk CdS and CdS/unmodified MXene hybrid catalysts. The enhanced HER activity and stability of the designed heterojunction catalyst are attributed to the presence of oxygen-containing terminal groups on the surface of thermally treated Ti_(3)C_(2)T_(x) MXene, extended light absorption spectra as well as the precisely constructed intimate Schottky contact, implying an accelerated interfacial charge transfer and efficient, long-term photocatalytic hydrogen production performance. The results demonstrate that oxygen-terminated 2D MXene can be well utilized as a functional platform for the development of novel heterojunction photocatalysts.

关键词： CdS MXene heterojunction water splitting hydrogen evolution reaction

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Exploring Picturebooks Through the Model of Double-Scope Stories

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Language and Semiotic Studies 2017年第1期3卷 114-124页

作者： Xiaofei Shi Soochow University China

This article investigates the picturebook as the aesthetic object that integrates the verbal narrative and the visual narrative, and hinges on interactions between the two levels of narratives. Often drawing on the theory of semiotics, picturebook scholars have revealed the complex interactions between the verbal and the visual, the demands that a picturebook may put on the reader, and the hermeneutic nature of picturebook reading. Two signifi cant issues, however, still need to be further explored: why the reader can possibly relate and integrate the verbal and the visual, and why some readers may turn to pictures more than to words, or vice versa. I propose to explore the two issues primarily through the model of double-scope stories within the theoretical framework of cognitive criticism. I argue that the universality of the intellectual and imaginative activity of conceptual blending makes it possible for the reader to relate pictures to words, or vice versa, and to integrate them. Though to make meaning from the picturebook as a double-scope story requires the reader to integrate the two levels of narratives, the verbal and the visual can sometimes constitute two relatively independent input stories. Readers may be more oriented towards either one of them, partially due to the difference in their innate cognitive structures. The arguments will be illustrated with a close analysis of Lane Smith's picturebook Grandpa Green.

关键词： picturebook cognitive criticism conceptual blending double-scope story

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Convolutional Neural Networks of Whole Jujube Fruits Prediction Model Based on Multi-Spectral Imaging Method

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Chinese Journal of Electronics 2023年第3期32卷 655-662页

作者： WANG Jing FAN xiaofei shi Nan ZHAO Zhihui SUN Lei SUO Xuesong College of Mechanical and Electrical Engineering Hebei Agricultural University Key Laboratory of Microbial Diversity Research and Application of Hebei Province College of Life Sciences Hebei University Research Center of Chinese Jujube Hebei Agricultural University

Soluble sugar is an important index to determine the quality of jujube, and also an important factor to influence the taste of jujube. The acquisition of the soluble sugar content of jujube mainly relies on manual chemical measurement which is time-consuming and labor-intensive. In this study, the feasibility of multispectral imaging combined with deep learning for rapid nondestructive testing of fruit internal quality was analyzed. Support vector machine regression model, partial least squares regression model, and convolutional neural networks(CNNs) model were established by multispectral imaging method to predict the soluble sugar content of the whole jujube fruit, and the optimal model was selected to predict the content of three kinds of soluble sugar. The study showed that the sucrose prediction model of the whole jujube had the best performance after CNNs training, and the correlation coefficient of verification set was 0.88, which proved the feasibility of using CNNs for prediction of the soluble sugar content of jujube fruits.

关键词： Jujube Multi-spectral imaging Convolutional neural networks

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DOA Estimation Algorithm with Non-uniform Motion Synthetic Linear Array

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Transactions of Nanjing University of Aeronautics and Astronautics 2018年第S1期35卷 75-80页

作者： shi Zhan Zhang xiaofei College of Electronic and Information Engineering Nanjing University of Aeronautics and Astronautics State Key Laboratory of Millimeter Waves Southeast University

Synthetic aperture using moving array is widely used for extending array aperture and improving direction of arrival(DOA)estimation performance.This paper proposes a DOA estimation algorithm with non-uniform motion synthetic linear array.The proposed method successively estimates the phase correction factors of the signals received by a moving linear array and then compensates the manifold to construct a much larger synthetic array.With the synthetic array,the DOA estimation performance is efficiently improved.Besides,due to the successive phase correction factors estimation,the proposed method can be applied regardless of the configuration and motion of the linear array and can release the request of temporal signal coherence.The simulations validate the effectiveness of the proposed method.

关键词： DOA estimation synthetic array moving array

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