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Reciprocal regulation between lunapark and atlastin facilitates ER three-way junction formation

Protein & Cell 2019年第7期10卷 510-525页

作者： Xin Zhou xiaofang huang Yuting Guo Dong Li Junjie Hu Department of Genetics and Cell Biology College of Life Sciences Nankai University and Tianjin Key Laboratory of Protein Sciences Tianjin 300071 China National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Science Beijing 100101 China University of Chinese Academy of Sciences Beijing 100101 China

Three-way junctions are characteristic structures of the tubular endoplasmic reticulum (ER) network. Junctions are formed through atlastin (ATL)-mediated membrane fusion and stabilized by lunapark (Lnp). However, how Lnp is preferentially enriched at three-way junctions remains elusiveHere, we showed that Lnp loses its junction localization when ATLs are deleted. Reintroduction of ATL1 R77A and ATL3, which have been shown to cluster at the junctions, but not wild-type ATL1, relocates Lnp to the junctions. Mutations in the Nmyristoylation site or hydrophobic residues in the coiled coil (CC1) of Lnp N-terminus (NT) cause mis-targeting of LnpConversely, deletion of the lunapark motif in the C-terminal zinc fin ger domain, which affects the homooligomerization of Lnp, does not alter its localizationPurified Lnp-NT attaches to the membrane in a myristoylation- dependent manner. The mutation of hydrophobic residues in CC1 does not affect membrane association, but compromises ATL interactionsIn addition, Lnp-NT inhibits ATL-mediated vesicle fusion in vitro. These results suggest that CC1 in Lnp-NT contacts junction-enriched ATLs for proper localization;subsequently, further ATL activity is limited by Lnp after the junction is formed. The proposed mechanism ensures coordinated actions of ATL and Lnp in generating and maintaining three-way junctions.

关键词： endoplasmic reticulum three-way junction membrane fusion lunapark atlastin amphipathic helix myristoylation

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Molecular characterization and expression of the SiUCP2 gene in sea urchin Strongylocentrotus intermedius

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Journal of Oceanology and Limnology 2021年第4期39卷 1523-1537页

作者： Lingshu HAN Zijiao QUAN Bing HAN Beichen DING xiaofang huang Heng WANG Yaqing CHANG Jun DING Key Laboratory of Mariculture&Stock Enhancement in North China's Sea Ministry of Agriculture and Rural Aff airsDalian Ocean UniversityDalian 116023China

Uncoupling protein 2 (UCP2) is a proton transporter located in the inner mitochondrial membrane, and inhibits the formation of adenosine triphosphate and reactive oxygen species by uncoupling oxidative phosphorylation. To provide a theoretical basis for the role of SiUCP2 in lipid metabolism, a 2 341-bp full-length cDNA of SiUCP2 from sea urchin Strongylocentrotus intermedius , which encodes 323 amino acids (predicted MW 36.11 kDa) was obtained, and the structure and function of the SiUCP2 gene and its expression at the mRNA and protein level were studied. SiUCP2 had high homology with UCP2 of other species. Expression of SiUCP2 was detected in the order of tube feet > gonads > coelomocytes > intestines. The expression level was the highest in prismatic larvae and lowest in the two-cell stage. Moreover, using in-situ hybridization, we found that SiUCP2 protein was expressed in the gonads and intestine. This study provided a theoretical basis for subsequent studies on the role of SiUCP2 and its regulatory mechanism in lipid metabolism, and for the improvement of gonad quality to obtain a higher economic value from sea urchins.

关键词： Strongylocentrotus intermedius SiUCP2 gene cloning lipid metabolism in-situ hybridization western blot

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Finite-Time Nonsmooth Vibration Control for Building Structure with Actuator Failures 36

Finite-Time Nonsmooth Vibration Control for Building Structu...

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第36届中国控制会议

作者： Wang Jianhui Wang Qing xiaofang huang Mingjing Yao School of Mechanical and Electric Engineering Guangzhou University School of Civil Eengineering Guangzhou University The Engineering & technical college of chengdu University of Technology

ISBN: (纸本)9781538629185

In order to accommodate actuator failures which are uncertain in time, pattern and value, the finite-time stable nonsmooth control method is applied to suppress the vibration, which overcomes the shortcomings of the traditional control method neglecting the influence of actuator. According to theory of Lyapnov stability, the designed control method is proved that can realize the stability of building structural vibration. Finally, several simulation examples are given for numerical simulation that regarding a building by the earthquake. The control effect under nonsmooth control condition is compared with a way of LQR control. The simulation results reveal that the designed control method has great seismic performance and is available for vibration control of actuator failure in building structure.

关键词： Building structure Finite-time stability Nonsmooth control Actuator failure Vibration

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Electrophysiological evaluation in 4 patients with diabetic pseudotabes

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Neural Regeneration Research 2007年第5期2卷 301-304页

作者： xiaofang Cai Qionghua huang Department of Neuroelectrophysiology Zhongshan Hospital Xiamen University Xiamen 361004 Fujian Province China

BACKGROUND： Researches on diabetic nervous system lesion are mainly focus on peripheral nerve and vegetative nerve, so there are few investigations on diabetic pseudotabes. OBJECTIVE： To investigate the electrophysiological examinations on the diagnosis of diabetic pseudotabes. DESIGN： Case study. SETTING： Department of Electrophysiology and Department of Neurology, Zhongshan Hospital Affiliated to Xiamen University. PARTICIPANTS： A total of 4 patients with type 2 diabetes mellitus, including 3 males and 1 female aged from 50 to 72 years, were selected from Department of Neurology, Zhongshan Hospital Affiliated to Xiamen University from March 2002 to February 2005. All accepted subjects met the modified diagnostic criteria of diabetes mellitus, which was set by American Diabetes Mellitus Association （ADA） in 1997. Otherwise, the subjects had typical symptoms and physical signs of spinal posterior funiculus damage. However, patients with spinal cord lesion which was caused by other factors were excluded. All accepted subjects provided the confirmed consent. METHODS： Nicolet NT electromyography （EMG）/evoked potential meter （made in the USA） was used to detect spinal cord conduction velocity （SCCV）, somatosensory evoked potential （SEP） of lower limbs, motor nerve conduction velocity （MNCV） and sensory nerve conduction velocity （SNCV） of extremities. Determining criteria： Measurements were performed based on the laboratory standards. SCCV, which was less than lower limit of normal value （T2–12： 40–55 m/s, T12–L4： 20–41 m/s, T2–L4： 36–45 m/s）, was regarded as abnormal. SEP value of lower limbs： P40, P60 and PF, which were more than standard deviation of normal value （x — ＋2.5）, were regarded as the abnormality. Normal value of P40, P60 and PF latencies （x —±s） in this study： P40, P60 and PF in males were （37.6±1.9） ms, （59.8±3.9） ms and （7.6±0.9） ms, respectively; meanwhile, those in females were （35.5±1.7） ms, （55.2±2.7） ms and （6.3±0.7） ms, respectively. MNCV and SNCV,

关键词： diabetes mellitus tabes dorsalis evoked potentials, somatosensory neural conduction electrophysiology

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Tumor extracellular vesicles mediate anti-PD-L1 therapyresistance by decoying anti-PD-L1

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Cellular & Molecular Immunology 2022年第11期19卷 1290-1301页

作者： Jiming Chen Jie Yang Wenhui Wang Danfeng Guo Chengyan Zhang Shibo Wang Xinliang Lu xiaofang huang Pingli Wang Gensheng Zhang Jing Zhang Jianli Wang Zhijian Cai Institute of Immunology and Department of Orthopaedics of the Second Affiliated HospitalZhejiang University School of Medicine310058 HangzhouChina Henan Key Laboratory for Digestive Organ Transplantation The First Affiliated Hospital of Zhengzhou University450052 ZhengzhouChina Department of Critical Care Medicine Qilu HospitalCheeloo College of MedicineShandong University250063 JinanChina Department of Respiratory and Critical Care Medicine The Second Affiliated Hospital of Zhejiang University School of Medicine310003 HangzhouChina Department of Critical Care Medicine of the Second Affiliated Hospital Zhejiang University School of Medicine310003 HangzhouChina Department of Pathology Zhejiang University First Affiliated Hospital and School of Medicine310002 HangzhouChina Institute of Immunology and Bone Marrow Transplantation Center of the First Affiliated HospitalZhejiang University School of Medicine310058 HangzhouChina Institute of Hematology Zhejiang University&Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy310006 HangzhouChina

PD-L1+tumor-derived extracellular vesicles(TEVs)cause systemic immunosuppression and possibly resistance to anti-PD-L1 antibody(αPD-L1)***,whether and how PD-L1+TEVs mediateαPD-L1 therapy resistance is ***,we show that PD-L1+TEVs substantially decoyαPD-L1 and that TEV-boundαPD-L1 is more rapidly cleared by macrophages,causing insufficient blockade of tumor PD-L1 and subsequentαPD-L1 therapy *** of endogenous production of TEVs by Rab27a or Coro1a knockout reversesαPD-L1 therapy *** an increasedαPD-L1 dose or macrophage depletion mediated by the clinical drug pexidartinib abolishesαPD-L1 therapy ***,in the treatment cycle with the same total treatment dose ofαPD-L1,high-dose and low-frequency treatment had better antitumor effects than low-dose and highfrequency treatment,induced stronger antitumor immune memory,and eliminatedαPD-L1 therapy ***,in humanized immune system mice with human xenograft tumors,both increasedαPD-L1 dose and high-dose and low-frequency treatment enhanced the antitumor effects ofα***,increased doses ofαPD-L1 andαPD-1 had comparable antitumor effects,butαPD-L1 amplified fewer PD-1+Treg cells,which are responsible for tumor ***,our results reveal a TEV-mediated mechanism ofαPD-L1-specific therapy resistance,thus providing promising strategies to improveαPD-L1 efficacy.

关键词： Tumor Extracellular vesicles PD-L1

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Spatial variability of cyanobacterial community composition in Sanya Bay as determined by DGGE fingerprinting and multivariate analysis

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Chinese Science Bulletin 2013年第17期58卷 1019-1027页

作者： LING Juan ZHANG YanYing DONG JunDe WANG YouShao huang Hui CHEN Lei huang xiaofang LONG LiJuan ZHANG Si Key Laboratory of Marine Bio-resourses Sustainable Utilization South China Sea Institute of OceanologyChinese Academy of Sciences State Key Laboratory of Tropical Oceanography South China Sea Institute of OceanologyChinese Academy of Sciences National Experiment Station of Tropical Marine Biology Chinese Academy of Sciences Graduate University of Chinese Academy of Sciences

The cyanobacterial communities in the surface and bottom waters of Sanya Bay were investigated on April 24 and 25,*** cytometry showed that the total cyanobacterial abundance in the surface and bottom layers ranged from 0.7×10 4 to 2.38×10 4 cells mL-1 and from 1×10 4 to 1.8×10 4 cells mL-1,*** diversity was analyzed using a molecular fingerprinting technique called denaturing gradient gel electrophoresis(DGGE),followed by DNA *** results were then interpreted through multivariate statistical *** in the compositions of cyanobacterial communities were observed in the surface and bottom waters at the same station,with some bands obtained from both the surface and bottom layers,whereas some bands were present only in one *** predominant cyanobacterial species of the excised DGGE bands were related to Synechococcus or Synechococcus-like species(56.2%).Other phylogenetic groups identified included Chroococcidiopsis(6.3%),Cyanobium(6.3%) and some unclassified cyanobacteria(31.2%).A redundancy analysis(RDA) was conducted to reveal the relationships between the cyanobacterial community composition and environmental *** results showed that the spatial variations in the cyanobacterial community composition in surface waters was significantly related to chlorophyll a(Chla),the biochemical oxygen demand(BOD),nitrate and phosphate(P<0.05).Meanwhile,the spatial variations in the bottom waters was significantly affected by nitrate,nitrite,and phosphate(P<0.05).Environmental parameters could explain 99.3% and 58.3% of the variations in the surface and bottom layers,respectively.

关键词：群落组成 DGGE 三亚湾空间变异性蓝藻多元分析指纹分析变性梯度凝胶电泳

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地方人大专委会制度及其完善——基于31个省级人大的实证分析

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教学与研究 2015年第6期 27-35页

作者：黄小钫北京市委党校政治学教研部北京100044

地方人大制度在地方治理体系中居于主导地位,是支撑地方治理体系和治理能力现代化的主要政治制度之一。地方人大专门委员会作为地方人大工作机制的重要组成部分,其设立主要源于地方人大及其常委会自身履行职权的需要,有助于发挥地方人... 详细信息

地方人大制度在地方治理体系中居于主导地位,是支撑地方治理体系和治理能力现代化的主要政治制度之一。地方人大专门委员会作为地方人大工作机制的重要组成部分,其设立主要源于地方人大及其常委会自身履行职权的需要,有助于发挥地方人大所担负的治理功能。因此,健全地方人大专门委员会制度,完善地方人大工作机制,是推进地方治理体系建设和提升地方治理能力的基本要求。但是,从实际情况来看,地方人大尤其是省级人大的专委会制度还存在法律不严密、数量不统一、称谓不规范、规模不一致等问题,因而亟须加强地方人大工作机制——即专委会制度建设,充分保障地方人大治理功能的有效发挥。

关键词：地方人大专门委员会制度建设

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我国省级人大常委会会次和会期制度研究——基于北京市的分析

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教学与研究 2017年第10期 50-60页

作者：黄小钫北京市委党校政治学教研部

会次和会期制度是人大常委会会议制度的重要内容,涉及会议召开的次数、会议举行的日期、会议持续的时间以及开会的间隔时间等内容。会次和会期制度作为一项程序性制度,其完善与否直接关系到地方人大常委会会议的质量和实效,进而影响地... 详细信息

会次和会期制度是人大常委会会议制度的重要内容,涉及会议召开的次数、会议举行的日期、会议持续的时间以及开会的间隔时间等内容。会次和会期制度作为一项程序性制度,其完善与否直接关系到地方人大常委会会议的质量和实效,进而影响地方国家权力机关法定职权的正常行使。为此,需要正确认识、把握会次和会期制度的民主价值,加强会次和会期制度的法律制度建设,逐步推进人大常委会组成人员专职化,不断提升人大常委会会议工作的法治化水平,保证地方国家权力机关充分履行职责。

关键词：人大常委会会次会期会议制度

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当代中国地方人大常委会党组:历史演进、结构特征及其运行机制

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教学与研究 2023年第5期 38-49页

作者：黄小钫中国人民大学中共党史党建学院北京100872

地方人大常委会党组是地方党委在地方国家权力机关设立的领导机构,发端于1979年地方人大设立人大常委会作为常设机关之际。设立地方人大常委会党组,是中国共产党作为最高政治领导力量和社会中心力量的内在要求,破解了党既要领导国家政... 详细信息

地方人大常委会党组是地方党委在地方国家权力机关设立的领导机构,发端于1979年地方人大设立人大常委会作为常设机关之际。设立地方人大常委会党组,是中国共产党作为最高政治领导力量和社会中心力量的内在要求,破解了党既要领导国家政权机关但又不能代替国家政权机关的困境,实现了党的领导与执政的有机统一。地方人大常委会党组领导作用的充分发挥,有赖于党组会议机制、请示报告机制、沟通协调机制以及团结联系机制的有效运行。新时代提高党领导人大工作的制度化、规范化和科学化水平,要全面认识并把握人大常委会党组的价值及意义,不断推进人大常委会党组制度建设。

关键词：党组党组制度地方党委地方人大常委会党组

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基于传统皮影特征的个性化皮影人物造型研究

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装饰 2019年第2期 140-141页

作者：黄小芳中国计量大学艺术与传播学院

皮影,是我国流传最为久远的民间戏剧之一,也是世界著名的非物质文化遗产之一。然而,随着科技的进步和社会的发展,尤其是新媒体的日益普及,传统皮影总体趋于没落,前景不容乐观。为了创新发展皮影艺术,本文构建了传统皮影人物数据库,将真... 详细信息

皮影,是我国流传最为久远的民间戏剧之一,也是世界著名的非物质文化遗产之一。然而,随着科技的进步和社会的发展,尤其是新媒体的日益普及,传统皮影总体趋于没落,前景不容乐观。为了创新发展皮影艺术,本文构建了传统皮影人物数据库,将真实人物形态、容貌与传统皮影特征相结合,进行个性化皮影人物造型设计,为皮影的造型研究提供了新的思路。

关键词：皮影非遗数据库个性化设计

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