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Sucrose-associated SnRK1a1-mediated phosphorylation of Opaque2 modulates endosperm filling in maize

Molecular Plant 2024年第5期17卷 788-806页

作者： Tao Yang Yunqin Huang Longyu Liao Shanshan Wang Haoyu Zhang Jingying Pan Yongcai Huang Xiaoling Li Di Chen Tao Liu xiaoduo lu Yongrui Wu State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China Institute of Molecular Breeding for Maize Qilu Normal UniversityJinanChina

During maize endosperm filling,sucrose not only serves as a source of carbon skeletons for storage-reserve synthesis but also acts as a stimulus to promote this ***,the molecular mechanisms underlying sucrose and endosperm filling are poorly *** this study,we found that sucrose promotes the expression of endosperm-filling hub gene Opaque2(O2),coordinating with storage-reserve *** showed that the protein kinase SnRK1a1 can attenuate O2-mediated transactivation,but sucrose can release this *** assays revealed that SnRK1a1 phosphorylates O2 at serine 41(S41),negatively affecting its protein stability and transactivation *** observed that mutation of SnRK1a1 results in larger seeds with increased kernel weight and storage reserves,while overexpression of SnRK1a1 causes the opposite *** of the native O2(O2-OE),phospho-dead(O2-SA),and phospho-mimetic(O2-SD)variants all increased 100-kernel *** O2-SA seeds exhibit smaller kernel size,they have higher accumulation of starch and proteins,resulting in larger vitreous endosperm and increased test weight.O2-SD seeds display larger kernel size but unchanged levels of storage reserves and test weight.O2-OE seeds show elevated kernel dimensions and nutrient storage,like a mixture of O2-SA and O2-SD ***,our study discovers a novel regulatory mechanism of maize endosperm *** of S41 as a SnRK1-mediated phosphorylation site in O2 offers a potential engineering target for enhancing storage-reserve accumulation and yield in maize.

关键词： maize Opaque2 SnRK1 endosperm phosphorylation sucrose

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Integration of high-throughput phenotyping,GWAS,and predictive models reveals the genetic architecture of plant height in maize

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Molecular Plant 2023年第2期16卷 354-373页

作者： Weixuan Wang Weijun Guo Liang Le Jia Yu Yue Wu Dongwei Li Yifan Wang Huan Wang xiaoduo lu Hong Qiao Xiaofeng Gu Jian Tian Chunyi Zhang Li Pu Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya 572024China Institute of Molecular Breeding for Maize Qilu Normal UniversityJinan 250200China Institute for Cellular and Molecular Biology The University of Texas at AustinAustinTX 78712USA Department of Molecular Biosciences The University of Texas at AustinAustinTX 78712USA Sanya Institute Hainan Academy of Agricultural SciencesSanya 572000China

Plant height(PH)is an essential trait in maize(Zea mays)that is tightly associated with planting density,biomass,lodging resistance,and grain yield in the *** the dynamics of maize plant architecture will be beneficial for ideotype-based maize breeding and prediction,as the genetic basis controlling PH in maize remains largely *** this study,we developed an automated high-throughput phenotyping platform(HTP)to systematically and noninvasively quantify 77 image-based traits(i-traits)and 20 field traits(f-traits)for 228 maize inbred lines across all developmental ***-resolved i-traits with novel digital phenotypes and complex correlations with agronomic traits were characterized to reveal the dynamics of maize *** i-trait-based genome-wide association study identified 4945 traitassociated SNPs,2603 genetic loci,and 1974 corresponding candidate *** found that rapid growth of maize plants occurs mainly at two developmental stages,stage 2(S2)to S3 and S5 to S6,accounting for the final PH *** integrating the PH-association network with the transcriptome profiles of specific internodes,we revealed 13 hub genes that may play vital roles during rapid *** candidate genes and novel i-traits identified at multiple growth stages may be used as potential indicators for final PH in *** candidate gene,ZmVATE,was functionally validated and shown to regulate PH-related traits in maize using genetic ***,machine learning was used to build predictive models for final PH based on i-traits,and their performancewas assessed across developmental ***,strong,and very strong correlations between predictions and experimental datasets were achieved from the early S4(tenth-leaf)***,our study provides a valuable tool for dissecting the spatiotemporal formation of specific internodes and the genetic architecture of PH,as well as resources and predictive models that are useful for molecular desig

关键词： phenomics GWAS plant height machine learning prediction maize

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Gene-lndexed Mutations in Maize

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Molecular Plant 2018年第3期11卷 496-504页

作者： xiaoduo lu Jisheng Liu Wen Ren Qun Yang Zhenguang Chai Rumei Chen Lei Wang Jun Zhao Zhihong Lang Haiyang Wang Yunliu Fan Jiuran Zhao Chunyi Zhang Institute of Molecular Breeding for Maize Qilu Normal University Jinan 250200 China Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing 100081 China Maize Research Center Beijing Academy of Agriculture & Forestry Sciences Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing100097 China These authors contributed equally to this article.

The availability of the B73 inbred reference genome sets the stage for high-throughput functional charac- terization of maize genes on a whole-genome scale. Among the 39 324 protein-coding genes predicted, the vast majority are untapped due to the lack of suitable high-throughput reverse genetic resources. We have generated a gene-indexed maize mutant collection through ethyl methanesulfonate mutagenesis and de- tected the mutations by combining exome capture and next-generation sequencing. A total of 1086 mutated MI plants were sequenced, and 195 268 CG〉TA-type point mutations, including stop gain/loss, missplice, start gain/loss, and various non-synonymous protein mutations as well as 4610 InDel mutations, were identified. These mutations were distributed on 32 069 genes, representing 82% of the predicted protein-coding genes in the maize genome. We detected an average of 180 mutations per mutant line and 6.1 mutations per gene. As many as 27 214 mutations of start codons, stop codons, or missplice sites were identified in 14 101 genes, among which 6232 individual genes harbored more than two such muta- tions. Application of this mutant collection is exemplified by the identification of the ent-kaurene synthase gene, which encodes a key enzyme in the gibberellin biosynthesis pathway. This gene-indexed genome- wide mutation collection provides an important resource for functional analysis of maize genes and may bring desirable allelic variants for genetic breeding in maize.

关键词： maize ethyl methanesulfonate mutation exome capture next-generation sequencing functional genomics

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Regulation of Leaf Angle by Auricle Development n Maize

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Molecular Plant 2017年第3期10卷 516-519页

作者： Fanying Kong Tingting Zhang Jisheng Liu Siqi Heng Qingbiao Shi Haisen Zhang Zeli Wang Lei Ge Pinghua Li xiaoduo lu Gang Li College of Life Science State Key Laboratory of Crop Biology Shandong AgricuLtural University Tai'an Shandong 271018 China College of Agronomy Shandong Agricultural University Tai'an Shandong 271018 China Institute of Molecular Breeding for Maize Qilu Normal University Jinan Shandong 250200 China These authors contributed equally to this article.

In plants, the leaf angle （LA） affects how densely crops can be planted and thus is one of factors determining grain yield per acre. This complex quantitative trait is controlled by developmental signals, hormones, ... 详细信息

关键词： Regulation Leaf Angle Auricle Development in Maize

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VAMP726 from maize and Arabidopsis confers pollen resistance to heat and UV radiation by influencing lignin content of sporopollenin

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Plant Communications 2023年第6期4卷 163-178页

作者： Wenqi Yang Dongdong Yao Haiyang Duan Junli Zhang Yaling Cai Chen Lan Bing Zhao Yong Mei Yan Zheng Erbing Yang xiaoduo lu Xuehai Zhang Jihua Tang Ke Yu Xuebin Zhang State Key Laboratory of Crop Stress Adaptation and Improvement Henan Joint International Laboratory for Crop Multi-Omics ResearchSchool of Life SciencesHenan UniversityKaifeng 475004China National Key Laboratory of Wheat and Maize Crop Science College of AgronomyHenan Agricultural UniversityZhengzhou 450002China The Shennong Laboratory Zhengzhou 450002China College of Chemistry Zhengzhou UniversityZhengzhou 450001China National Engineering Laboratory of Crop Stress Resistance School of Life ScienceAnhui Agricultural UniversityHefei 230036China

Sporopollenin in the pollen cell wall protects male gametophytes from *** derivatives,including guaiacyl(G)lignin units,are known to be structural components of sporopollenin,but the exact composition of sporopollenin remains to be fully *** analyzed the phenylpropanoid derivatives in sporopollenin from maize and Arabidopsis by thioacidolysis coupled with nuclear magnetic resonance(NMR)and gas chromatography–mass spectrometry(GC–MS).The NMR and GC–MS results confirmed the presence of p-hydroxyphenyl(H),G,and syringyl(S)lignin units in sporopollenin from maize and ***,H units account for the majority of lignin monomers in sporopollenin from these *** next performed a genome-wide association study to explore the genetic basis of maize sporopollenin composition and identified a vesicle-associated membrane protein(ZmVAMP726)that is strongly associated with lignin monomer composition of maize *** manipulation of VAMP726 affected not only lignin monomer composition in sporopollenin but also pollen resistance to heat and UV radiation in maize and Arabidopsis,indicating that VAMP726 is functionally conserved in monocot and dicot *** work provides new insight into the lignin monomers that serve as structural components of sporopollenin and characterizes VAMP726,which affects sporopollenin composition and stress resistance in pollen.

关键词： pollen cell wall sporopollenin lignin monomers heat stress UV radiation

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QTG-Seq Accelerates QTL Fine Mapping through QTL Partitioning and Whole-Genome Sequencing of Bulked Segregant Samples

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Molecular Plant 2019年第3期12卷 426-437页

作者： Hongwei Zhang Xi Wang Qingchun Pan Pei Li Yunjun Liu xiaoduo lu Wanshun Zhong Minqi Li Linqian Han Juan Li Pingxi Wang Dongdong Li Yan Liu Qing Li Fang Yang Yuan-Ming Zhang Guoying Wang Lin Li 1nstitute of Crop Science Chinese Academy of Agricultural SciencesBeijing 100081China National Key Laboratory of Crop Genetic Improvement Crop Information CenterHuazhong Agricultural UniversityWuhan 430070China Crop Information Center College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China lnstitute of Molecular Breeding for Maize Qilu Normal UniversityJinan 250200China

Deciphering the genetic mechanisms underlying agronomic traits is of great importance for crop improvement. Most of these traits are controlled by multiple quantitative trait loci (QTLs), and identifying the underlying genes by conventional QTL fine-mapping is time-consuming and labor-intensive. Here, we devised a new method, named quantitative trait gene sequencing (QTG-seq), to accelerate QTL fine-mapping. QTGseq combines QTL partitioning to convert a quantitative trait into a near-qualitative trait, sequencing of bulked segregant pools from a large segregating population, and the use of a robust new algorithm for identifying candidate genes. Using QTG-seq, we fine-mapped a plant-height QTL in maize (Zea mays L.), qPH7, to a 300-kb genomic interval and verified that a gene encoding an NF-YC transcription factor was the functional gene. Functional analysis suggested that qPH7-encoding protein might influence plant height by interacting with a CO-like protein and an AP2 domain-containing protein. Selection footprint ana卜 ysis indicated that qPH7 was subject to strong selection during maize improvement. In summary, QTG-seq provides an efficient method for QTL fine-mapping in the era of “big data".

关键词： quantitative trait locus QTL QTL fine-mapping whole genome sequencing plant height

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Identifications of aleurone- and starchy endosperm-specifically expressed transcription factors in rice

Identifications of aleurone- and starchy endosperm-specifica...

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2013全国植物生物学大会

作者： BAI Aining WU Xiaoba lu xiaoduo ZHANG Jindan LIU Jinxin LIU Chunming Institute of Botany Chinese Academy of Sciences

Rice(Oryza sativa L.)endosperm consists of four distinct functional domains:aleurone layer,starchy endosperm,transfer cells and embryo-surrounding *** aleurone layer and starchy endosperm have the same developmental origin,they are different from each other in morphology,biochemical composition and cell *** identify the key transcription factors regulating the differentiation of the aleurone layer and starchy endosperm,microarray analyses were performed in aleurone layer and starchy endosperm cells isolated in seeds 14 days after *** identified 949 transcription factors that were differentially expressed between these two ***-qPCR assays were used to validate the expression pattern, allowing us to identify 15 transcription factors highly expressed in the aleurone layer and 13 ones highly expressed in starchy endosperm *** carrying these genes’ promoter fused withβ-glucuronidase(GUS) were stably transformed into rice,the detailed expression patterns of these genes were analyzed *** elucidate the functions of these genes,we screened for mutants by Targeting Induce Local Lesions in Genome(TILLING) technology. We believe these studies will help to decipher how the development of aleurone and starchy endosperm are regulated in rice.

关键词： endosperm aleurone layer starchy endosperm cells microarray rice

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The Differential Transcription Network between Embryo and Endosperm in the Early Developing Maize Seed

The Differential Transcription Network between Embryo and En...

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第一届全国玉米生物学学术研讨会

作者： xiaoduo lu Dijun Chen Defeng Shu Zhao Zhang Weixuan Wang Christian Klukas Ling-ling Chen Yunliu Fan Ming Chen Chunyi Zhang 中国农业科学院生物技术研究所

Transcriptome analysis of early-developing maize(Zea mays)seed was conducted using Illumina *** mapped 11,074,508 and 11,495,788paired-end reads from endosperm and embryo,respectively,at 9 d after pollination to define gene structure and alternative splicing events as well as transcriptional regulators of gene expression to quantify transcript abundance in both embryo and

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拟南芥GATL12基因影响叶绿体的形成

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植物学报 2011年第4期46卷 379-385页

作者：苏延萍路小铎沈颂东张春义苏州大学基础医学与生物科学学院苏州215123 中国农业科学院生物技术研究所北京100081 中国科学院植物研究所信号转导与代谢组学研究中心北京100093 国家农作物基因资源与遗传改良重大科学工程北京100081

谷氨酰胺氨基转移酶(GATase)能够将谷氨酰胺上的氨基基团转移到底物上形成新的一碳氮基团。GATase有两种类型,即Class-I(trpG型)和Class-II(purF型)。拟南芥(Arabidopsis thaliana)基因组中有13个基因编码Class-I类似蛋白(GATLs),其生... 详细信息

谷氨酰胺氨基转移酶(GATase)能够将谷氨酰胺上的氨基基团转移到底物上形成新的一碳氮基团。GATase有两种类型,即Class-I(trpG型)和Class-II(purF型)。拟南芥(Arabidopsis thaliana)基因组中有13个基因编码Class-I类似蛋白(GATLs),其生物学功能尚不清楚。首先分离到拟南芥GATL12基因的2个T-DNA插入突变体,分别命名为gatl12-1和gatl12-2。然后观察发现在这2个突变体的杂合植株中,大部分植株的胚珠发育到第8天时,由于叶绿体的积累而呈现绿色,其余植株(约有25%)的胚珠为白色。将从杂合突变体植株上收获的种子播种在1/2MS培养基上,有25%的幼苗发育成黄化苗。经PCR检测,这些黄化苗为GATL12的纯合突变体,RT-PCR法在黄化苗中检测不到GATL12基因的转录本。电镜观察表明,突变体中的叶绿体不能正常发育。上述结果表明,GATL12基因在拟南芥的叶绿体发育过程中具有重要作用。

关键词：拟南芥叶绿体发育胚胎发生谷氨酰胺氨基转移酶

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多元醇还原法制备Fe3O4纳米粒子及磁性能表征

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化工新型材料 2015年第1期43卷 166-168,172页

作者：蒋丽萍邱建华韦谷韫陆小朵广西师范大学药用资源化学与药物分子工程教育部重点实验室化学与药学学院桂林541004

采用简便的多元醇还原法合成Fe3O4磁性纳米颗粒,研究了稳定剂类型、初始反应温度、初始反应时间和熟化时间对Fe3O4纳米颗粒形貌、粒径及磁性能的影响。采用XRD、TEM、SQUID多种表征手段对Fe3O4纳米颗粒进行表征,结果表明:聚乙二醇作为... 详细信息

采用简便的多元醇还原法合成Fe3O4磁性纳米颗粒,研究了稳定剂类型、初始反应温度、初始反应时间和熟化时间对Fe3O4纳米颗粒形貌、粒径及磁性能的影响。采用XRD、TEM、SQUID多种表征手段对Fe3O4纳米颗粒进行表征,结果表明:聚乙二醇作为稳定剂制备的Fe3O4纳米具有高结晶度以及单分散性,在300K下,具有超顺磁性和较高的饱和磁化强度;十二烷基磺酸钠和十六烷基溴化铵作为稳定剂却无法得到Fe3O4。稳定剂油酸/油胺的摩尔浓度比为1∶1时,升高初始反应温度从125℃到200℃,对Fe3O4的粒径和磁性能有决定性的影响;延长初始反应时间和熟化时间有利于提高纳米颗粒的分散性。

关键词： Fe3O4纳米颗粒多元醇还原法稳定剂 1,2-十四烷二醇超顺磁性

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