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Antifungal Activity of Compounds Isolated from Bamboo Vermicompost against <i>Rhizoctonia solani</i>AG1-IB

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Advances in Microbiology 2019年第12期9卷 957-970页

作者： xiaodong you Daigo Wakana Kazuki Ishikawa Tomoo Hosoe Motoaki Tojo Graduate School of Life and Environmental Sciences Osaka Prefecture University Sakai Osaka Japan Department of Organic Chemistry Hoshi University Shinagawa Tokyo Japan Research Institute of Pharmaceutical Sciences Musashino University Nishitokyo-Shi Tokyo Japan

Vermicompost has become a promising alternative to chemical pesticide because of its disease suppression effect during these decades. However, the mechanisms by which vermicompost suppress plant disease are not well-understood. Antifungal compounds, which are released by beneficial organisms in the vermicompost, may play an important role in inhibiting plant pathogens;however, these mechanisms have not been widely examined. In this study, Sterilized Water Extracts of bamboo vermicompost (SWE), using a 0.22-μm cellulose acetate filter, significantly (P < 0.05) inhibited the mycelium growth of Rhizoctonia solani AG1-IB on a Potato Dextrose Agar (PDA) plate. This suggests that antifungal compounds are present in bamboo vermicompost. The ethanol acetate (EtOAc) crude extracts of bamboo vermicompost showing antifungal activity were further separated. Two compounds were isolated from the EtOAc fraction of bamboo vermicompost and characterized as ergosterol peroxide (1) and (22E, 24R)-5α,8α-epidioxyergosta-6,9(11),22-trien-3β-ol (2). Their chemical structures and mass spectra were determined by nuclear magnetic resonance and mass spectrometry analyses. Ergosterol peroxide tested at 150, 300, 600, and 900 μg showed 13%, 22%, 34%, and 53% mycelial growth inhibition against R. solani AG1-IB, respectively. Because EtOAc crude extracts of the initial substrate materials of bamboo vermicompost did not inhibit mycelium growth of R. solani AG1-IB, antifungal compounds in the vermicompost may be released by microbes but not from the original substrates during vermicomposting.

关键词： Antifungal Compound Ergosterol Peroxide Vermicompost Rhizoctonia solani AG1-IB

IMMUNOMODULATING EFFECTS OF THE FUCOIDAN-GALACTOSAN SULFATE (F_12) ON MACROPHAGES IN MICE

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Chinese Journal of Reactive Polymers 2001年第1期10卷 73-76页

作者： ZHANG Guozhi you xiaodong WANG Lei SONG Jianqiu WANG Hairen Yantai Kangtai Medicine Product Corp. Shandong province 264006 College of Life Science Shandong UniversityJinan 250100

The immunomodulating effects of F12 on mouse peritoneal macrophages was intended to be studied with different doses of 20, 40, 80 and 120mg/Kg. F12 increased peroxidase activity in dexc dependent mammer from 0.02±0.001 to 0.175±0.038, 0.211±0.041, 0.137±0.045 and 0.078±0.036, respectively, increased cytotoxicity from 127.33±22.96 to 162.74±19.53, 237.30±25.15 , 178.62±36.22 and 158.66±42.75dpm, respectively, promoted phagocytic activity from 0.03±0.01 to0.21±0.016, 0.43±0.041, 0.40±0.032 and 0.30±0.160b. Furthermore, F12 in a concentration of 15, 30,60,120μg/ml enhanced IL-1 production from 0.15±0.02 to 0.20±0.005b 0.21±0.02,0.22±0.005,0.24±0.002 and 0.22±0.02,respectively. Thus F12 can be considered as an effective stimulator of macrophages.

关键词： Fucoidan-Galactosan sulfate Macrophage Tumor Cytotoxicity Interleukin-1.

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推陈出新的办刊秘笈世界杂志的创新报告

编辑学报 2017年第2期29卷 196-199页

作者：张晓冬游苏宁 <中华放射学杂志>编辑部中华医学会继续教育部北京100710

通过阅读《2016—2017世界杂志媒体创新报告》,文章从独特的视角、全球的视野介绍2016—2017年世界杂志媒体创新的发展前沿。编者精心挑选并高度概括了当今世界杂志媒体的主要亮点。文章认为:应通过重塑文化为创新奠基,从而应对广告拦... 详细信息

通过阅读《2016—2017世界杂志媒体创新报告》,文章从独特的视角、全球的视野介绍2016—2017年世界杂志媒体创新的发展前沿。编者精心挑选并高度概括了当今世界杂志媒体的主要亮点。文章认为:应通过重塑文化为创新奠基,从而应对广告拦截的软件危机;通过构建易于使用的数据库,充分发挥智能数据的无穷潜力;通过综述微支付模式的日臻成熟、移动唯一的势不可挡、原生广告的迅猛来袭,总结出有助于我们探囊取物以实现盈利的他山之石。

关键词：杂志媒体创新报告

计算机模拟——儿童解决算术应用题的认知行为发展模型(Ⅰ)

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心理科学 1991年第2期14卷 22-27,64页

作者：刘凡尤晓东中央教育科学研究所北京航空航天大学计算机系

本研究采用计算机模拟的方法,对儿童解决简单加减法应用题的认知行为进行了系统的分析,建立了一个信息加工的表征转换模型。这个模型解释了问题解决过程中各种知识图式组织和处理数量信息的作用,并且详细地描述了儿童问题解决行为的三... 详细信息

本研究采用计算机模拟的方法,对儿童解决简单加减法应用题的认知行为进行了系统的分析,建立了一个信息加工的表征转换模型。这个模型解释了问题解决过程中各种知识图式组织和处理数量信息的作用,并且详细地描述了儿童问题解决行为的三种能力水平。

关键词：儿童算术应用题问题情境解决问题计算机模拟数量关系相依关系数学表征问题解决认知行为

基于生态文明视角下我国国土综合整治的思路与建议

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南方农业 2019年第24期13卷 91-93,105页

作者：尤晓东陕西地建土地勘测规划设计院有限责任公司陕西土地工程建设集团有限责任公司

国土综合整治是生态文明建设的重要内容之一,依靠科学的综合整治方法可以有效提高土地生产力,实现资源的再配置和可持续发展.基于此,通过简述国土综合整治和生态文明建设的内涵、现状及其相互关系,分析国内外土地资源综合整治的现状和问... 详细信息

国土综合整治是生态文明建设的重要内容之一,依靠科学的综合整治方法可以有效提高土地生产力,实现资源的再配置和可持续发展.基于此,通过简述国土综合整治和生态文明建设的内涵、现状及其相互关系,分析国内外土地资源综合整治的现状和问题,探索国土综合整治需要坚持的原则,进而提出推进我国国土综合整治的思路与建议.

关键词：生态文明国土综合整治可持续发展