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Engineered extracellular vesicles efficiently deliver CRISPR-Cas9 ribonucleoprotein(RNP)to inhibit herpes simplex virus1 infection in vitro and in vivo

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1362-1379页

作者： Yuanda Wan Liren Li Ruilin Chen Jiajia Han Qiyun Lei Zhipeng Chen xiaodong tang Wenyu Wu Shuwen Liu Xingang Yao NMPA Key Laboratory for Research and Evaluation of Drug Metabolism Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Center of Clinical Pharmacy Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Key Laboratory of Infectious Diseases Research in South China(Southern Medical University) Ministry of EducationGuangzhou 510515China

Extracellular vesicles(EVs)have recently emerged as a promising delivery platform for CRISPR/Cas9 ribonucleoproteins(RNPs),owing to their ability to minimize off-target effects and immune responses.However,enhancements are required to boost the efficiency and safety of Cas9 RNP enrichment within EVs.In response,we employed the Fc/Spa interaction system,in which the human Fc domain was fused to the intracellular domain of PTGFRN-Δ687 and anchored to the EV membrane.Simultaneously,the B domain of the Spa protein was fused to the C domain of cargos such as Cre or spCas9.Due to the robust interaction between Fc and Spa,this method enriched nearly twice the amount of cargo within the EVs.EVs loaded with spCas9 RNP targeting the HSV1 genome exhibited significant inhibition of viral replication in vitro and in vivo.Moreover,following neuron-targeting peptide RVG modification,the in vivo dosage in neural tissues substantially increased,contributing to the clearance of the HSV1 virus in neural tissues and exhibiting a lower off-target efficiency.These findings establish a robust platform for efficient EV-based SpCas9 delivery,offering potential therapeutic advantages for HSV1 infections and other neurological disorders.

关键词： Extracellular vesicles CRISPR/Cas9 Ribonucleoproteins PTGFRN Fc/Spa HSV1 Neuron-targeting Delivery

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A Survey of Statistical Relationships between Tropical Cyclone Genesis and Convectively Coupled Equatorial Rossby Waves

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Advances in Atmospheric Sciences 2022年第5期39卷 747-762页

作者： Shuguang WANG Juan FANG xiaodong tang Zhe-Min TAN Key Laboratory of Mesoscale Severe Weather(MOE) School of Atmospheric SciencesNanjing UniversityNanjing 210093China

Convectively coupled equatorial Rossby waves(ERW)modulate tropical cyclone activities over tropical oceans.This study presents a survey of the statistical relationship between intraseasonal ERWs and tropical cyclone genesis(TCG)over major global TC basins using four-decade-long outgoing longwave radiation(OLR)and TC best-track datasets.Intraseasonal ERWs are identified from the OLR anomalies using an empirical orthogonal function(EOF)analysis method without imposing equatorial symmetry.We find that westward-propagating ERWs are most significant in four tropical ocean basins over the summer hemisphere and that ERWs exhibit similar northeast-southwest(southeast-northwest)tilted phase lines in the northern(southern)hemisphere,with an appreciable poleward advance of wave energy in most TC basins.The EOF-based ERW indices quantitatively show that ERWs significantly modulate TC genesis.The convectively active(suppressed)phases of ERWs coincide with increased(reduced)TCG occurrences.The TCG modulation by ERWs achieves the maximum where the ERWs propagate through the climatological TCG hotspots.As a result,the total number of TCG occurrences in the TC basins varies significantly according to the ERW phase.The ERW-TCG relationship is significant over the northwestern Pacific Ocean,northeastern Pacific Ocean,and the northern Indian Ocean during the northern summer seasons.In the southern summer season,the ERW-TCG relationship is significant over the southern Indian Ocean,Indonesian-Australia basin,and the southwestern Pacific Ocean.However,ERW activities are weak in the main TC development region of the Atlantic Ocean;and the impact on Atlantic TCG appears to be insignificant.

关键词： tropical cyclone genesis equatorial Rossby waves statistical analysis

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Associative learning of a three-terminal memristor network for digits recognition

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Science China(Information Sciences) 2023年第2期66卷 209-218页

作者： Yiming REN Bobo TIAN Mengge YAN Guangdi FENG Bin GAO Fangyu YUE Hui PENG xiaodong tang Qiuxiang ZHU Junhao CHU Chungang DUAN Key Laboratory of Polar Materials and Devices (MOE) Ministry of Education Department of ElectronicsEast China Normal University Zhejiang Lab Institute of Microelectronics Beijing Innovation Center for Future Chips (ICFC) Tsinghua University Collaborative Innovation Center of Extreme Optics Shanxi University

Imitating the associative intelligence of the biological brain is attractive but is poorly achieved in hardware because the complex tunable connection in neural networks is difficult to reproduce. We develop a circuit composed of a three-terminal memristor network to reproduce the biological conditioning process artificially. The synaptic weight between co-firing neurons is strengthened simultaneously by generating a feedback signal from the integrate-and-fire neuron to the gate of the synaptic memristor. The network allows the multi-associative capacity of recalling more than one digit in one circuit. Both single and multi-associative learning for recalling digital images are achieved. Furthermore, all 10 digital images from “0” to “9” are successfully recalled in an associative network with such paralleling circuits. Assisted by this associative layer, a typical classification network effectively improves the recognition rate for fragmentary digital images.Our work sheds light on brain-inspired artificial associative memory and provides a strategy for applications,such as object recognition with partial features and similar scenes.

关键词： artificial intelligence associative learning memristor classification network

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Direct regeneration of LiNi_(0.5)Co_(0.2)Mn_(0.3)O_(2) cathode material from spent lithium-ion batteries

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Chinese Journal of Chemical Engineering 2021年第12期34卷 278-286页

作者： xiaodong tang Qiankun Guo Miaomiao Zhou Shengwen Zhong Faculty of Materials Melallurgy and Chemistry Jiangxi University of Science and TechnologyGanzhou 341000China

At present,metal ions from spent lithium-ion batteries are mostly recovered by the acid leaching procedure,which unavoidably introduces potential pollutants to the environment.Therefore,it is necessary to develop more direct and effective green recycling methods.In this research,a method for the direct regeneration of anode materials is reported,which includes the particles size reduction of recovered raw materials by jet milling and ball milling,followed by calcination at high temperature after lithium supplementation.The regenerated LiNi_(0.5)Co_(0.2)Mn_(0.3)O_(2) single-crystal cathode material possessed a relatively ideal layered structure and a complete surface morphology when the lithium content was n(Ni+Co+Mn):n(Li)=1:1.10 at a sintering temperature of 920 0 C,and a sintering time of 12 h.The first discharge specific capacity was 154.87 mA·h·g^(-1) between 2.75 V and 4.2 V,with a capacity retention rate of 90% after 100 cycles.

关键词： Spent lithium-ion batteries LiNi_(0.5)Co_(0.2)Mn_(0.3)O_(2)cathode material Direct regeneration

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分离多环芳烃用于超稠油掺稀降黏的研究

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石油炼制与化工 2024年第3期55卷 55-60页

作者：唐晓东李小雨杨谨郑存川西南石油大学化学化工学院成都610500 重庆市自来水有限公司渝中区水厂

针对炼油厂催化裂化柴油大量过剩和芳烃含量过高等问题,结合稠油掺稀降黏开采掺稀比高、稀油资源紧缺的现状,从催化裂化柴油中萃取分离多环芳烃,用于超稠油掺稀降黏研究。对多环芳烃萃取进行正交试验,在筛选出的最佳操作条件(萃取温度... 详细信息

针对炼油厂催化裂化柴油大量过剩和芳烃含量过高等问题,结合稠油掺稀降黏开采掺稀比高、稀油资源紧缺的现状,从催化裂化柴油中萃取分离多环芳烃,用于超稠油掺稀降黏研究。对多环芳烃萃取进行正交试验,在筛选出的最佳操作条件(萃取温度为45℃、萃取时间为5 min、相分离时间为5.5 min和剂油体积比为1.4)下,多环芳烃产品收率为29.87%、芳烃质量分数高达99.07%。在掺稀比均为0.10时,多环芳烃和塔河稀油的掺稀降黏量率分别为94.20%和68.58%。拟合计算结果表明,多环芳烃用量仅为塔河稀油的16.32%时,即可达到塔河油田稠油掺稀降黏要求。以塔河油田稠油自喷井为例,当稠油含水率分别为0,30%和50%时,掺入多环芳烃相对掺入稀油的单井日产油增长率分别达到115.90%,84.57%,62.20%。

关键词：塔河超稠油催化裂化柴油多环芳烃塔河稀油掺稀降黏

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A surgical simulation system for predicting facial soft tissue deformation

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Computational Visual Media 2016年第2期2卷 163-171页

作者： xiaodong tang Jixiang Guo Peng Li Jiancheng Lv Machine Intelligence Lab Sichuan University West China College of Stomatology Sichuan University

In the field of cranio-maxillofacial(CMF)surgery, surgical simulation is becoming a very powerful tool to plan surgery and simulate surgical results before actually performing a CMF surgical procedure.Reliable prediction of facial soft tissue changes is in particular essential for better preparation and to shorten the time taken for the operation. This paper presents a surgical simulation system to predict facial soft tissue changes caused by the movement of bone segments during CMF surgery. Two experiments were designed to test the feasibility of this simulation system. The test results demonstrate the feasibility of fast and good prediction of post-operative facial appearance, with texture. Our surgical simulation system is applicable to computer-assisted CMF surgery.

关键词： cranio-maxillofacial(CMF) surgery finite element model(FEM) soft tissue model view-controller

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Lightweight Body-In-White Design Driven by Optimization Technology

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Automotive Innovation 2018年第3期1卷 255-262页

作者： Heguo Ou xiaodong tang Jieping Xiao Yuanbo Wang Zhongmin Ma Off-Road Vehicle R&D Beijing Automotive Industry CorporationNo.99 Shuanghe StreetShunyi DistrictBeijingChina

Structural optimization technologies are increasingly applied to lightweight automotive structural design.In a body-inwhite(BIW)development project,optimization technologies are applied in three steps that are seamlessly integrated into engineering practice.By following a design-driven approach,an innovative design that was 50.65 kg lighter than the base design was achieved.First,topology optimization was used to investigate the optimal material distribution in a given design space,and helped in extracting the main load path,while considering crashworthiness,NVH,and static stiffness performance requirements.In the second step,the effective design of a beam cross-section and feasible joints was carried out based on optimization technology and expert knowledge.In this step,the deep involvement of manufacturing process experts on stamping and joint connection feasibility analysis increased the chance of success remarkably.The sensitivity analysis,topography optimization,and gauge optimization of the created draft 3D BIW frame comprised the last step before finalizing the initial design.The new design was validated by full load case simulations,including full vehicle crashworthiness analysis,mode frequency,and body mount point dynamic stiffness analysis,and BIW stiffness and component attachment stiffness analysis.The validation results indicate that the new BIW design achieves the performance goal,while being 13.3%lighter than the base design.

关键词： BIW design Lightweight design Structural optimization Optimization technology

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基于三维地质模型的路桥工程特殊地质岩体勘察方法研究

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中国高新科技 2024年第7期 39-41页

作者：唐小东甘肃中科华建规划设计发展有限公司甘肃兰州730030

路桥工程施工前对其地质条件尤其是特殊地质岩体进行勘察具有积极意义,甚至影响未来的工程设计与施工。由于特殊地质岩体的复杂性和不确定性,传统勘察方式存在效率较低、精度不高、人为影响较大等弊端难以准确掌握其性质和特征。因此,... 详细信息

路桥工程施工前对其地质条件尤其是特殊地质岩体进行勘察具有积极意义,甚至影响未来的工程设计与施工。由于特殊地质岩体的复杂性和不确定性,传统勘察方式存在效率较低、精度不高、人为影响较大等弊端难以准确掌握其性质和特征。因此,需要研究新的勘察方法。基于此,文章重点针对3种三维地质模型在路桥工程中的特殊地质岩体勘察方法进行介绍,旨在为后续实践工作提供指导。

关键词：三维地质模型路桥特殊地质岩体勘察

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路桥工程勘察中地基土体液化判别方法设计研究

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科技创新与生产力 2024年第6期45卷 112-114页

作者：唐小东甘肃中科华建规划设计发展有限公司甘肃兰州730030

本文指出土体液化直接影响地基质量,是路桥工程的重要勘察项目之一;相较于传统的经验方法,利用标准贯入试验对土体液化进行判别更为精准、可靠。本文以此为目标,在梳理基本技术原理的基础上,重点对其数理模型进行推演与拟合,并利用工程... 详细信息

本文指出土体液化直接影响地基质量,是路桥工程的重要勘察项目之一;相较于传统的经验方法,利用标准贯入试验对土体液化进行判别更为精准、可靠。本文以此为目标,在梳理基本技术原理的基础上,重点对其数理模型进行推演与拟合,并利用工程案例对其具体流程进行了设计与应用,旨在为后续勘察工作中的地基土体判别提供依据与参考。

关键词：路桥工程勘察地基液化贯入试验

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媒体融合背景下广电经营性业务竞争力提升对策探析

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新闻研究导刊 2024年第5期15卷 251-253页

作者：唐小东山西广播电视台交通广播节目中心山西太原030013

近年来,随着通信技术的进步和传媒科技的发展,新媒体以其便捷性、快速性、分享性等特点迅速崛起,并给以报纸、广播、电视为代表的传统媒体带来了巨大冲击。面对严峻的竞争格局,传统媒体纷纷革新传播方式,积极采用新技术,搭建新平台。新... 详细信息

近年来,随着通信技术的进步和传媒科技的发展,新媒体以其便捷性、快速性、分享性等特点迅速崛起,并给以报纸、广播、电视为代表的传统媒体带来了巨大冲击。面对严峻的竞争格局,传统媒体纷纷革新传播方式,积极采用新技术,搭建新平台。新媒体经过一段“野蛮扩张”时期后,也积极融入传统媒体优秀因素,做优传播内容,组建专业采编运营队伍等。如今,媒体融合已成为无处不在、影响深远的媒体现象、社会现象。在我国,政府主导、行业推动、各媒体积极参与将媒体融合推到一个全新的高度。在媒体融合背景下,各媒体单位应积极抓住发展机遇期,努力提升“内功”,推动自身发展壮大。文章采用案例分析法,以传统媒体中的广电媒体为例,围绕产品生产、运营管理、传播渠道和受众关系四个方面,有针对性地提出通过“独特性+创新性”来提升产品竞争力;通过“资源整合+多元盈利”来提升运营竞争力;通过“营销创新+开放拓展”来提升渠道竞争力;通过“粉丝营销+社区营销”来提升受众管理竞争力等策略。文章认为,面对新媒体带来的冲击与挑战,广电媒体必须转变发展理念,强化服务意识,做好内容生产与形式创新,全面提高品牌知名度和影响力,最终实现品牌效益的最大化。

关键词：媒体融合广电媒体经营性业务竞争力策略

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