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A highly sensitive DNA-AIEgen-based ＂turn-on＂ fluorescence chemosensor for amplification analysis of Hg^2+ ions in real samples and living cells

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Science China Chemistry 2017年第5期60卷 663-669页

作者： Xiaowen OU xiaoding lou Fan Xia Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica School of Chemistry and Chemical Engineering Huazhong University of Science and Technology Wuhan 430074 China Shenzhen Institute of Huazhong University of Science and Technology Shenzhen 518000 China

Functional nucleic acids(FNAs)-based biosensors have shown great potential in heavy metal ions detection due to their low-cost and easy to operate merits. However, in most FNAs based fluorescence probes, the ingenious designs of double-labeled(fluorophore and quencher group) DNA sequence, not only bring the annoyance of organic synthesis, but also restrict its use as a robust biosensor in practical duties. In this paper, we design a simple AIEgens functional nucleic acids(AFNAs) probe which consists of only fluorogen but no quencher group. With the help of duplex-specific nuclease(DSN) enzyme based target recycling, high fluorescence signal and superior sensitivity towards Hg^(2+) are achieved. This robust assay allows for sensitive and selective detection of Hg^(2+) in real water samples and mapping of intracellular Hg^(2+), without double-labeling of oligonucleotide with a dye-quencher pair, nor the multiple assay steps.

关键词： functional nucleic acids AIEgen Hg2+ detection real samples living cells

DDTC-Na-based colorimetric chemosensor for the sensing of cyanide in water

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Science China Chemistry 2009年第6期52卷 802-808页

作者： lou xiaoding LI Zhen QIN JinGui Department of Chemistry Hubei Key Laboratory on Organic and Polymeric Optoelectronic MaterialsWuhan UniversityWuhan 430072China

Sodium diethyldithiocarbamate (DDTC-Na) was demonstrated to be a new colorimetric cyanide chemosensor by utilizing an indirect trick. First, some copper ions were added to the colorless aque- ous solution of DDTC-Na. Then, the resultant brown solution was studied upon the addition of different anions, including Cl-, I-, IO3-, SO42-, NO-2, Br-, H2PO4-, F-, SCN-, HSO-4, ClO-4 and CN-. It was observed by naked eyes that the brown solution changed to colorless immediately after the addition of the trace cyanide, but there were no changes towards other anions, making DDTC-Na a good selective cyanide chemosensor in pure water.

关键词： colorimetric cyanide chemosensor indirect trick DDTC-Na

DNA hybridization chain reaction and DNA supersandwich self-assembly for ultrasensitive detection

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Science China Chemistry 2017年第3期60卷 311-318页

作者： Nannan Liu Fujian Huang xiaoding lou Fan Xia Key Laboratory of Carbon Materials of Zhejiang Province College of Chemistry & Materials EngineeringWenzhou UniversityWenzhou 325027China Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica School of Chemistry and Chemical EngineeringHuazhong University of Science and Technology CAS Key Laboratory of Soft Matter Chemistry Department of Polymer Science and EngineeringUniversity of Science and Technology of China

The fabrication of sensitive sensors with high selectivity is highly desirable for the detection of some important biomarkers,such as nucleic acids,proteins,small molecules and *** hybridization chain reaction(HCR) and DNA supersandwich self-assembly(SSA) are two prevalent enzyme-free signal amplification strategies to improve sensitivity of the *** this review,we firstly describe the characteristics about DNA HCR and DNA SSA,and then summarize the advances in the one-dimensional DNA nanostructures assisted by HCR and *** review has been divided into three parts according to the two signal amplification methods and highlights recent progress in these two strategies to improve the detection sensitivity of proteins,nucleic acids,small molecules and ions.

关键词： signal amplification method hybridization chain reaction supersandwich self-assembly ultrasensitive detection

Rational Fabrication and Biomedical Application of Biomolecule-Conjugated AIEgens through Click Reaction

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Chinese Journal of Chemistry 2019年第10期37卷 1072-1082页

作者： Qiming Yuan Yong Cheng xiaoding lou Fan Xia Engineering Research Center of Nano-Geomaterials of Ministry of Education Faculty of Materials Science and ChemistryChina University of GeosciencesWuhanHubei 430074China State Key Laboratory of Material Processing and Die&Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhanHubei 430074China Zhejiang Institute China University of GeosciencesHangzhouZhejiang 311305China【摘要】Contents

Recently,the issue of cancer has attracted extensive *** diagnosis and timely therapy are important for cancer *** lots of advanced fluorescent probes have been applied to cancer ***,the further development of these probes is limited by the disadvantages of poor targeting,weak sensitivity and ***,the emergence of biomolecule-conjugated fluorescent probes with aggregationinduced emission properties has taken innovative impetus to the cancer *** review summarizes the rational fabrication and biomedical applications of biomolecule-conjugated A1E luminogens(AIEgens)based on"click reaction"over the past *** the meantime,the challenges of biomolecule-conjugated AIEgens in the field of biomedicine are also discussed.

关键词： AIE diagnosis Rational

Multifunctional aggregates for precise cellular analysis

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Science China Chemistry 2021年第11期64卷 1938-1945页

作者： Jing-Jing Hu Xiaoqi Dong Wenlian Jiang Fan Xia xiaoding lou State Key Laboratory of Biogeology and Environmental Geology Faculty of Materials Science and ChemistryChina University of GeosciencesWuhan430074China

Precisely analyzing the target materials in living cells can reveal the essence and mystery of life at a deeper level,which will provide reliable theoretical basis for the occurrence,development,treatment and prognosis of major ***,because living cells are in the dynamic process of metabolism,there are several challenges existed in accurate analysis,including subcellular compartment heterogeneity,plasma membrane interface barrier,and cell cycle *** this regard,our group has designed and synthesized a series of multifunctional aggregates by mainly integrating the peptide elements,nucleic acid elements and aggregation elements to overcome the *** article summarizes the latest developments of multifunctional aggregates for precise cell analysis by our group,and systematically introduces them according to different design concepts and targeting dimensions,such as space,efficiency as well as *** hope this work could contribute to analyzing the biomarkers in cells through constructing the multifunctional aggregates,understanding the operation mechanism of cells,finally inspiring technology breakthroughs in biomedical fields.

关键词： precise cellular analysis aggregation induced emission peptide nucleic acid aggregate

Recent Development of DNA-modified AIEgen Probes for Biomedical Application

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Chemical Research in Chinese Universities 2021年第1期37卷 66-72页

作者： HU Qinyu WU Jun CHEN Lulu lou xiaoding XIA Fan Engineering Research Center of Nano-Geomaterials of the Ministry of Education Faculty of Materials Science and ChemistryChina University of GeosciencesWuhan430074P.R.China

A variety of DNA-based probes are utilized for the detections of multiple analytes and DNA nanotechnology has been thriving for recent decades and achieving numerous nanostructures,mainly focusing on DNA morphology modulation and multifunctional systems engineered into to the complicated *** the numerous detections,fluorescence method is a non-invasive,highly selective and sensitive means for varieties of applications,but their emissions are often compromised by the aggregation-caused quenching(ACQ)effect,which weakens their *** aggregation induced emission luminogens(AIEgens)are created with non emissive or weakly emissive in a low concentration but emit strong fluorescence in a high concentration with aggregated ***,numerous functionalized AIEgens have been emerged and used for detection and imaging and DNA-modified AIEgen probes are *** this vein,here we report the progress on DNA-modified AIEgen probes in recent years and highlight their conjugation strategies including covalent bonding,electrostatic interaction and their applications of ***,multiple DNA strands are needed to introduce into the DNA-modified AIEgen probes for more *** the end,some challenges are mentioned to discuss the new trend of DNA-modified AIEgen probes.

关键词： Aggregation induced emission luminogen(AIEgen) DNA-modified probe Detection Imaging Biomedical application

One-dimensional and two-dimensional nanomaterials for the detection of multiple biomolecules

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Chinese Chemical Letters 2019年第9期30卷 1557-1564页

作者： Quan Wang Xudong Wang Min Xu xiaoding lou Fan Xia Engineering Research Center of Nano-Geomaterials of Ministry of Education Faculty of Materials Science and Chemistry China University of Geosciences Wuhan 430074 China Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica School of Chemistry and Chemical Engineering Huazhong University of Science and Technology Wuhan 430074 China Zhejiang Institute China University of Geosciences Wuhan Hangzhou 311305 China

The complexity of biological samples determines that the detection of a single biomolecule is unable to satisfy actual needs. Moreover, the "false positives" results caused by a single biomolecule detections easily leads to erroneous clinical diagnosis and treatment. Thus, it is important for the homogenous quantification of multiple biomolecules in not only basic research but also practical application. As a consequent, a large number of literatures have been exploited to monitor multiple biomolecules in homogenous solution, enabling facilitating the development of the disease diagnosis, treatment as well as drug discovery. One-dimensional nanomaterials and two-dimensional nanomaterials have special physical and chemical properties, such as good electrochemical properties, stable structure, large specific surface area, and biocompatibility, which are widely used in electrochemical and fluorescent detection of biomolecules. This tutorial review highlights the recent development for the detection of multiple biomolecules by using nanomaterials including one-dimensional materials(1DMs) as well as twodimensional materials(2DMs).

关键词： One-dimensional nanomaterials Two-dimensional nanomaterials Detection Multiple targets Biomolecules

Nanopores/Nanochannels Based on Electrical and Optical Dual Signal Response for Application in Biological Detection

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Chinese Journal of Chemistry 2023年第11期41卷 1374-1384页

作者： Guangwen Lu Niya Lin Zhaojun Chen Wenlian Jiang Jing-Jing Hu Fan Xia xiaoding lou State Key Laboratory of Biogeology and Environmental Geology Faculty of Materials Science and ChemistryChina University of GeosciencesWuhanHubei430078 China

Cancers and chronic diseases have always been global health problems. The occurrence and development of such diseases are closely related to the abnormalities of proteins, nucleic acids, ions or small molecules in the body. Nowadays, nanopores/nanochannels have emerged as a powerful platform for detecting these biomolecules based on the electrical signal variation caused by biomolecules passing. However, detection relied on the electrical signal easily suffered from the clogging defects, low throughput, and strong background signals. Fortunately, the emergence of designing nanopores/nanochannels based on electrical and optical dual signal response has brought innovative impetus to biological detection, which can also identify the chemical compositions and conformations of the biomolecules. In this review, we summarize the reasonable preparation of nanopores/nanochannels with electrical and optical dual signal response and their application in biological detection. According to different biomolecules, we divide the targets into four types, including nucleic acids, small molecules, ions and proteins. In each section, the design of representative examples and the principle of dual signal generation are introduced and discussed. Finally, the prospects and challenges of nanopores/nanochannels based on electrical and optical dual signal response are also discussed.

关键词： Biomolecules Nanopores nanochannels Electrical and optical Fluorescence lon current

An AIEgens and exonuclease Ⅲ aided quadratic amplification assay for detecting and cellular imaging of telomerase activity

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Science Bulletin 2017年第14期62卷 997-1003页

作者： Xuehong Min Lei Xia Yuan Zhuang Xudong Wang Jie Du Xiaojin Zhang xiaoding lou Fan Xia Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica School of Chemistry and Chemical Engineering Huazhong University of Science and Technology College of Materials and Chemistry Engineering Hainan University Faculty of Material Science and Chemistry China University of Geosciences Shenzhen Institute of Huazhong University of Science & Technology

Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis. In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens mod- ified probe （TPE-Py-DNA） as a fluorescence reporter and exonuclease llI （Exo lIl） as a signal amplifier. With the aid of telomerase, repeat units （TrAGGG）n are extended from the end of template substrate oligonucleotides （TS primer） that form duplex DNAs with TPE-Py-DNA. Then, Exo llI catalyzes the diges- tion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal. The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally, we obtain the target-to-signal amplification ratio of 1 ：N2. This strategy exhibits good performance for detecting clinical urine samples （distinguishing 15 cancer patients＇ samples from 8 healthy ones） and checking intracellular telomerase activity （differentiating cell lines including HeLa, MDA-MB-231, MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug）, which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer.

关键词： Quadratic amplification AIEgens Telomerase Urine samples Exonuclease Ill Cell imaging