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STAT3 mediates resistance of CD44^+CD24^(-/low) breast cancer stem cells to tamoxifen in vitro

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The Journal of Biomedical Research 2012年第5期26卷 325-335页

作者： Xiaoyan Wang Guozhu Wang Yi Zhao xiaoan liu Qiang Ding Jingping Shi Yin Ding Shui Wang Department of Breast Surgery the First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu 210029 China Laboratory of Mesoseopw Chemistry and Department of Polymer Science and Engineering College of Chemistry and Chemical Engineering Nanjing University Nanjing Jiangsu 210093 China

We sought to determine whether STAT3 mediated tamoxifen resistance of breast cancer stem cells in *** capacities for mammosphere formation and STAT3 expression of CD44＋CD24-/low MCF-7 and MCF-7 were *** CD44＋CD24-/low subpopulation ratio and its sensitivity to adriamycin were analyzed in MCF-7 and TAM resistant（TAM-R） *** cycle,apoptosis,STAT3 and phospho-STAT3 changes were observed af-ter treatment with *** interference RNA-mediated knockdown of STAT3 in TAM-R cells was also ***44＋CD24-/low MCF-7 showed higher capacities for mammosphere formation and STAT3 expression than total *** CD44＋CD24-/low subpopulation was also upregulated in TAM-R cells with less sensitivity to adriamycin than *** cycle changes,anti-apoptotic effects and STAT3 changes were also ***-while,the knock-down of STAT3 in TAM-R resulted in an increase in sensitivity to *** is concluded that STAT3 plays an essential role in breast cancer stem cells,which correlated with tamoxifen resistance.

关键词： STAT3 breast cancer cancer stem cells tamoxifen drug resistance

Hypoxia-induced factor-1 alpha upregulates vascular endothelial growth factor C to promote lymphangiogenesis and angiogenesis in breast cancer patients

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The Journal of Biomedical Research 2013年第6期27卷 478-485页

作者： Xiaojian Ni Yingchun Zhao Jingjing Ma Tiansong Xia xiaoan liu Qiang Ding Xiaoming Zha Shui Wang Department of Breast Surgery The First Affiliated Hospital of Nanjing Medical University Department of Breast Surgery The Second People's Hospital Affiliated with Wannan Medical College State Key Laboratory of Reproductive Medicine Department of Breast Surgery Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University

Hypoxia-induced factor-1 alpha （HIF-1α） affects many effector molecules and regulates tumor lymphangio- genesis and angiogenesis during hypoxia. The aim of this study was to investigate the role of HIF-1α in the regu- lation of vascular endothelial growth factor C （VEGF-C） expression and its effect on lymphangiogenesis and an- giogenesis in breast cancer. Lymphatic vessel density （LVD）, microvessel density （MVD） and the expressions of HIF-1α and VEGF-C proteins were evaluated by immunohistochemistry in 75 breast cancer samples. There was a significant correlation between HIF-1α and VEGF-C （P = 0.014, r = 0.273, Spearman＇s coefficient of correlation）. HIF-1α and VEGF-C overexpression was significantly correlated with higher LVD （P = 0.003 and P = 0.017, re- spectively）, regional lymph nodal involvement （P = 0.002 and P = 0.004, respectively） and advanced tumor, node, metastasis （TNM） classification （P = 0.001 and P = 0.01, respectively）. Higher MVD was observed in the group expressing higher levels of HIF-1α and VEGF-C （P = 0.033 and P = 0.037, respectively）. Univariate analysis showed shorter survival time in patients expressing higher levels of HIF-1α and VEGF-C. HIF-1α was also found to be an independent prognostic factor of overall survival in multivariate analysis. The results suggest that HIF-1α may affect VEGF-C expression, thus acting as a crucial regulator of lymphangiogenesis and angiogenesis in breast cancer. This study highlights promising potential of HIF- 1α as a therapeutic target against tumor lymph node me- tastasis.

关键词： HIF-1α VEGF-C lymphangiogenesis angiogenesis breast cancer

The cancer-testis gene,MEIOB,sensitizes triple-negative breast cancer to PARP1 inhibitors by inducing homologous recombination deficiency

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Cancer Biology & Medicine 2021年第1期18卷 74-87页

作者： Yayun Gu Cheng Wang Rongxuan Zhu Jianshui Yang Wenwen Yuan Yanhui Zhu Yan Zhou Na Qin Hongbing Shen Hongxia Ma Hongxia Wang xiaoan liu Zhibin Hu State Key Laboratory of Reproductive Medicine Center for Global HealthSchool of Public HealthNanjing Medical UniversityNanjing 211166China Jiangsu Key Lab of Cancer Biomarkers Prevention and TreatmentJiangsu Collaborative Innovation Center for Cancer Personalized MedicineNanjing Medical UniversityNanjing 211116China Department of Bioinformatics School of Biomedical Engineering and InformaticsNanjing Medical UniversityNanjing 211166China Department of Oncology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Department of Breast Surgery The First Affiliated Hospital with Nanjing Medical UniversityNanjing 210029China

Objective:The newly defined cancer-testis(CT)gene,MEIOB,was previously found to play key roles in DNA double-strand break(DSB)*** this study,we aimed to investigate the effects and mechanisms of MEIOB in the carcinogenesis of triple-negative breast cancers(TNBCs).Methods:The Cancer Genome Atlas database was used to quantify the expression of *** regression analysis was used to evaluate the association between MEIOB expression and the prognosis of human *** effects of MEIOB on cell proliferation and migration in TNBCs were also assessed in ***-derived xenograft(PDX)models were used to assess the sensitivity of breast cancers with active MEIOB to PARP1 ***:We confirmed MEIOB as a CT gene whose expression was restricted to the testes and breast tumors,especially *** activation was significantly associated with poor survival in breast cancer patients[overall,hazard ratio(HR)=1.90(1.16–2.06);TNBCs:HR=7.05(1.16–41.80)].In addition,we found that MEIOB was oncogenic and significantly promoted the proliferation of TNBC *** analysis showed that MEIOB participated in DSB repair in ***,in contrast to its function in meiosis,it mediated homologous recombination deficiency(HRD)through the activation of poly ADP-ribose polymerase(PARP)1 by interacting with ***,activated MEIOB was shown to confer sensitivity to PARP inhibitors,which was confirmed in PDX ***:MEIOB played an oncogenic role in TNBC through its involvement in *** addition,dysregulation of MEIOB sensitized TNBC cells to PARP inhibitors,so MEIOB may be a therapeutic target of PARP1 inhibitors in TNBC.

关键词： Cancer-testis gene MEIOB triple-negative breast cancer PARP1 inhibitor cell proliferation

Detection of micrometastases in bone marrow and sentinel lymph nodes of breast cancer patients

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Journal of Nanjing Medical University 2007年第1期21卷 32-35页

作者： Jia Zhao xiaoan liu Lijun Ling Department of General Surgery the First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

To study the sensitivity and clinical significance of HE-staining,IHC and RT-PCR in detecting breast cancer micrometastases in bone marrow and sentinel lymph nodes （SLNs）. Methods ：After general anesthesia, all patients underwent bone marrow puncture and sentinel lymph node biopsy （SLNB） by 1% isosulfan blue, and then HE-staining,IHC and RT-PCR were used to detect micrometastases. Results：Of 62 patients with breast cancer whose axillary lymph nodes showed negative HE-staining results, 15 cases presented with positive RT-PCR and 9 cases showed positive IHC results positive in bone marrow micrometastases detection. PT-PCR and IHC showed good uniformity（kappa=0.6945）and there was significant difference in detective rate between these two methods （X2=4.1667,P = 0.0412）. In SLN samples, 13 showed positive RT-PCR results, while 7 showed positive IHC results. PT-PCR and IHC showed good uniformity （kappa=0.64.83）and significant difference was also found in detective rate between these two methods （X^2=4.1667 ,P = 0.0412）. Both bone marrow and SLN samples were RT-PCR positive in 3 cases, which indicated that bone marrow did not always accompany SLN micrometastases（X^2=0.067,P = 0.796）. Conclusion： Even if no axillary lymph node involvement or distant metastases are present in routine preoperative examination, micrometastases can still be detected in bone marrow or SLNs. Because the bone marrow micrometastases and axillary node micrometastses are not present simultaneously, combination test of multiple indicators will detect micrometastases more accurately.

关键词： breast cancer micrometastases bone marrow sentinel lymph nodes cytokeratin

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基于卷积神经网络的中文景点识别研究

计算机工程与应用 2020年第4期56卷 140-145页

作者：刘小安彭涛北京联合大学智慧城市学院北京100101 北京联合大学机器人学院北京100101

命名实体识别是自然语言处理任务的重要环节。近年来,基于深度学习的通用命名实体识别模型取得显著效果。而在旅游领域,中文旅游景点实体识别主要依赖于特征工程的方法。提出一种基于CNN-BiLSTM-CRF的网络模型,该模型不使用任何人工特征... 详细信息

命名实体识别是自然语言处理任务的重要环节。近年来,基于深度学习的通用命名实体识别模型取得显著效果。而在旅游领域,中文旅游景点实体识别主要依赖于特征工程的方法。提出一种基于CNN-BiLSTM-CRF的网络模型,该模型不使用任何人工特征,通过神经网络充分对文本的局部信息特征进行抽象化抽取和表示,并学习和利用文本的上下文信息,实现对景点实体的识别。实验结果显示,该方法能够有效识别中文旅游景点实体,并在实验中取得F1值93.9%的效果。

关键词：中文命名实体识别深度学习景点识别卷积神经网络(CNN) 双向长短记忆网络(BiLSTM) 条件随机场(CRF)

脂质体介导C-erbB-2反义寡核苷酸转染小鼠乳腺癌TM40D细胞的效率及毒性

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江苏医药 2006年第8期32卷 757-759页

作者：刘晓安武正炎南京医科大学第一附属医院普通外科 210029

目的探讨阳离子脂质体为载体的反义寡核苷酸(ODNs)转染小鼠乳腺癌TM40D细胞的最佳转染效率及对细胞的毒性作用。方法分别将有/无脂质体介导的反义ODNs转染小鼠乳腺癌TM40D细胞,应用流式细胞仪观察不同时间的反义ODNs的转染效率,荧光显... 详细信息

目的探讨阳离子脂质体为载体的反义寡核苷酸(ODNs)转染小鼠乳腺癌TM40D细胞的最佳转染效率及对细胞的毒性作用。方法分别将有/无脂质体介导的反义ODNs转染小鼠乳腺癌TM40D细胞,应用流式细胞仪观察不同时间的反义ODNs的转染效率,荧光显微镜观察反义ODNs在细胞内的分布,全自动生化分析仪检测转染培养上清液的乳酸脱氢酶(LDH)浓度。结果无脂质体介导的反义ODNs转染细胞效率随着时间的延长增加,6h达到63.00%。脂质体介导的反义ODNs转染细胞4h时转染效率达到高峰为72.23%。细胞内荧光强度与细胞转染效率相关。无脂质体介导的FAM标记的反义ODNs分布在细胞浆,脂质体介导的反义ODNs在细胞浆及细胞核内均有分布。各组LDH浓度无统计学差异。结论脂质体介导组反义ODNs转染效率高于无脂质体组,同时转染高峰提前出现。脂质体促进反义ODNs进入细胞核内。短时间内阳离子脂质体及反义ODNs对细胞的毒性不明显。

关键词：脂质体 TM40D细胞反义寡核苷酸

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基于多特征多分类器集成的专利自动分类研究

数据分析与知识发现 2017年第8期1卷 76-84页

作者：贾杉杉刘畅孙连英刘小安彭涛北京联合大学智慧城市学院北京100101 北京联合大学机器人学院北京100101 北京联合大学城市轨道交通与物流学院北京100101

【目的】为了准确地给专利申请书分配IPC分类号,本文提出一种基于多特征多分类器集成的专利自动分类方法。【方法】使用从专利申请书中提取的全词典TFIDF特征、信息增益词典TFIDF特征、段落向量特征、主题模型向量特征,分别训练朴素贝... 详细信息

【目的】为了准确地给专利申请书分配IPC分类号,本文提出一种基于多特征多分类器集成的专利自动分类方法。【方法】使用从专利申请书中提取的全词典TFIDF特征、信息增益词典TFIDF特征、段落向量特征、主题模型向量特征,分别训练朴素贝叶斯、支持向量机、AdaBoost分类器,以此构建特征–类别矩阵,并结合F1权重矩阵集成,获得最终IPC预测分类号。【结果】对2014年–2016年"发动机或泵"领域的10个小类进行分类,使用Top Prediction、All Categories和Two Guesses三种评估方法得到准确率分别为:78.9%、80.1%、91.2%。【局限】训练仅仅使用了2014年–2016年共三年的专利数据,数据规模有限。【结论】在"发动机或泵"领域,本文方法能够有效地提高专利文本分类的准确率。

关键词：专利分类段落向量主题向量分类器集成

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连云港市养老保险基金支撑能力探究

淮海工学院学报（人文社会科学版） 2017年第12期15卷 107-109页

作者：刘肖安连云港市人力资源和社会保障局社会劳动保险管理处江苏连云港222006

随着我国人口老龄化、城镇化、经济市场化和就业多元化,部分地区养老保险基金收不抵支,养老保险基金支撑能力下降,基金收支矛盾已成为制约社会保障事业持续发展的一大瓶颈。阐述了连云港市基本养老保险基金收支状况及存在的问题,在分析... 详细信息

随着我国人口老龄化、城镇化、经济市场化和就业多元化,部分地区养老保险基金收不抵支,养老保险基金支撑能力下降,基金收支矛盾已成为制约社会保障事业持续发展的一大瓶颈。阐述了连云港市基本养老保险基金收支状况及存在的问题,在分析其原因的基础上,提出了增强养老保险基金支撑能力的对策。

关键词：养老保险基金收支矛盾支撑能力连云港市

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提升退休人员社会化管理服务水平研究

淮海工学院学报（人文社会科学版） 2017年第11期15卷 104-106页

作者：刘肖安连云港市人力资源和社会保障局社会劳动保险管理处江苏连云港222006

持续提高企业退休人员实行社会化管理服务水平是新时期党和政府赋予社会保障部门的工作任务。随着人口老龄化进程的加快,迫切需要持续提升社会化管理服务工作水平。从体制机制、队伍建设、退管经费、政策落实等社会化管理服务方面问题... 详细信息

持续提高企业退休人员实行社会化管理服务水平是新时期党和政府赋予社会保障部门的工作任务。随着人口老龄化进程的加快,迫切需要持续提升社会化管理服务工作水平。从体制机制、队伍建设、退管经费、政策落实等社会化管理服务方面问题进行探讨,为更好地实现退休人员老有所养、老有所乐、老有所医、老有所为,提出了相应对策。

关键词：退休人员社会化管理服务水平退管经费