检索结果-南通市图书馆

近红外二区光驱动的光动力治疗在克服肿瘤乏氧环境中的研究进展

在线全文

学校读者我要写书评

暂无评论

应用化学 2024年第7期41卷 925-936页

作者：杨懿芊严晓霞彭皓吴爱国杨方上海大学化学系上海200444 中国科学院宁波材料技术与工程研究所先进诊疗材料与技术实验室中国科学院宁波材料技术与工程研究所宁波315201 先进能源科学与技术广东省实验室惠州516000

光动力疗法因其具有非侵入性、低毒性、高时空选择性和特异靶向性等独特优势而被广泛应用于肿瘤治疗。该疗法利用光敏剂、光和氧分子,通过光敏剂介导产生活性氧自由基导致氧化损伤选择性地引起靶细胞的凋亡和坏死。然而,传统光动力疗法... 详细信息

光动力疗法因其具有非侵入性、低毒性、高时空选择性和特异靶向性等独特优势而被广泛应用于肿瘤治疗。该疗法利用光敏剂、光和氧分子,通过光敏剂介导产生活性氧自由基导致氧化损伤选择性地引起靶细胞的凋亡和坏死。然而,传统光动力疗法仍存在一些缺点,如光的组织穿透性有限、分子氧依赖以及肿瘤对治疗的固有障碍,这在很大程度上限制了肿瘤治疗效果。近些年来,国内外科研团队积极开发各种新型光敏剂,并将其与近红外光激发相结合,使得光动力疗法能够更深入地作用于肿瘤组织。针对深层肿瘤组织乏氧环境,进一步采取改善、利用该肿瘤微环境的策略,克服深层肿瘤组织缺氧对光动力疗法的限制,有助于优化肿瘤抑制效果。本文在阐明光动力疗法工作机制的基础上,系统总结讨论了近5年来近红外二区激光激发的光动力疗法在克服肿瘤乏氧障碍的最新进展,包括增加肿瘤组织内部氧含量和通过耐受缺氧环境2种方式针对性开发新型光敏剂,并对其发展前景进行了展望。

关键词：纳米材料光动力治疗肿瘤治疗近红外二区乏氧

维普期刊数据库博看期刊

Effects of Graded Hypothermia on Hypoxic-ischemic Brain Damage in the Neonatal Rat

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2011年第1期26卷 49-53页

作者： xiao-yan xia Yi-xin xia Department of Obstetrics Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing 100026 China Department of Obstetrics and Gynecology General Hospital of People's Armed Police Forces Beijing 100039 China

Objective To investigate the effect of graded hypothermia on neuropathologic alterations of neonatal rat brain after exposed to hypoxic-ischemic insult at 37℃, 33℃, 31℃, and 28℃, respectively, and to observe the effect of hypothermia on 72-kDa heat shock protein （HSP72） expression after hypoxic-ischemic insult. Methods Seven days old Wistar rats were subjected to unilateral common carotid artery ligation followed by exposure to hypoxia in 8% oxygen for 2 hours at 37℃, 33℃, 31℃, and 28℃, respectively. The brain temperature was monitored indirectly by inserting a mini-thermocouple probe into the temporal muscle during hypoxia. After hypoxia-ischemia their mortality was assessed. Neuronal damage was assessed with HE staining 72 hours after hypoxia. HSP72 expression at 0.5, 24, and 72 hours of recovery was immunohistochemically assessed using a monoclonal antibody to HSP72. Results Hypoxia-ischemia caused 10.5% （2 / 19） of mortality in rat of 37℃ group, but no death oc- curred in 33℃, 31℃ or 28℃ groups. HE staining showed neuropathologic damage was extensive in rats exposed to hypoxia-ischemia at 37℃ （more than 80.0%）. The incidence of severe brain damage was significantly decreased in 33℃ （53.3%） and 31℃ groups （44,4%）, and no histologic injury was seen in the 28℃ group of rats. Expression of HSP72 was manifest and persistent in the rat brain of 37℃ group, but minimum in the rat brain of 28℃ group. Conclusion Mild and moderate hypothermia might prevent cerebral visible neuropathologic damage associated with hypoxic-ischemic injury by decreasing stress response.

关键词： hypoxia-ischemia brain rat hypothermia heat shock protein 72

A Fast Calculation of Metric Scores for Learning Bayesian Network

在线全文

学校读者我要写书评

暂无评论

International Journal of Automation and computing 2012年第1期9卷 37-44页

作者： Qiang Lv xiao-yan xia Pei-De Qian School of Computer Science and Technology Soochow University Suzhou 215006 PRC Jiangsu Provincial Key Lab for Computer Information Processing Technology Suzhou 215006 PRC

Frequent counting is a very so often required operation in machine learning algorithms. A typical machine learning task, learning the structure of Bayesian network （BN） based on metric scoring, is introduced as an example that heavily relies on frequent counting. A fast calculation method for frequent counting enhanced with two cache layers is then presented for learning BN. The main contribution of our approach is to eliminate comparison operations for frequent counting by introducing a multi-radix number system calculation. Both mathematical analysis and empirical comparison between our method and state-of-the-art solution are conducted. The results show that our method is dominantly superior to state-of-the-art solution in solving the problem of learning BN.

关键词： Frequent counting radix-based calculation ADtree learning Bayesian network metric score

在线全文

学校读者我要写书评

暂无评论

雷公藤甲素诱导肺癌细胞铜死亡的机制研究

中国医药生物技术 2024年第2期19卷 135-143页

作者：肖艳霞刘璞郭君林雅军北京医院国家老年医学中心/国家卫生健康委北京老年医学研究所卫健委老年医学重点实验室/中国医学科学院老年医学研究院 100730

目的探讨雷公藤甲素诱导肺癌细胞铜死亡的分子机制。方法培养人肺癌细胞系A549和H460,采用MTT法检测细胞活力和EdU实验检测细胞增殖能力;Transwell迁移实验检测细胞迁移能力;铜离子荧光探针检测细胞内铜浓度;JC-1荧光探针检测细胞线... 详细信息

目的探讨雷公藤甲素诱导肺癌细胞铜死亡的分子机制。方法培养人肺癌细胞系A549和H460,采用MTT法检测细胞活力和EdU实验检测细胞增殖能力;Transwell迁移实验检测细胞迁移能力;铜离子荧光探针检测细胞内铜浓度;JC-1荧光探针检测细胞线粒体膜电位变化;Western blot检测铁硫簇蛋白FDX1、POLD1、SDHB、LIAS和脂酰化蛋白DLAT单体及寡聚体蛋白表达水平,以及铜稳态调节蛋白CTR1、ATP7A、ATP7B表达水平。结果 MTT实验显示,雷公藤甲素呈浓度依赖性抑制肺癌细胞活力,对A549和H460细胞半抑制浓度(IC50)分别为74.67nmol/L和34.12nmol/L;Ed U实验和Transwell迁移实验证实,雷公藤甲素可显著抑制肺癌细胞增殖与迁移能力(P <0.05);在雷公藤甲素处理后,在荧光显微镜下观察到A549和H460细胞内铜浓度上升,线粒体膜电位下降。进一步通过Western blot实验发现,A549和H460细胞铁硫簇蛋白FDX1、POLD1、SDHB、LIAS和脂酰化蛋白DLAT单体表达下调,DLAT寡聚体表达增加(P<0.05)。铜稳态调节蛋白CTR1表达无明显变化,ATP7A和ATP7B表达下调(P <0.05)。结论雷公藤甲素通过下调ATP7A和ATP7B表达,使细胞内铜浓度增加,进而下调铁硫簇蛋白表达水平、增加DLAT寡聚体表达水平,诱导肺癌细胞铜死亡,抑制肺癌细胞增殖与迁移。

关键词：雷公藤甲素肺癌铜死亡线粒体膜电位

Changes of Nerve Growth Factor in Amniotic Fluid and Correlation with Ventriculomegaly

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Sciences Journal 2011年第2期26卷 109-112页

作者： xiao-yan xia Xing-hua Huang Yi-xin xia Wei-hua Zhang Department of Obstetrics Beijing Obstetrics and Gynecology Hospital Capital Medical University Beijing 100026 China Department of Obstetrics and Gynecology General Hospital of Armed Police Forces Beijing 100039 China

Objective To detect the change of nerve growth factor (NGF) level in human amniotic fluid during gestation, and to explore the relationship between this change and fetal ventriculo-megaly (VM). Methods The studied subjects (collected from 2004 to 2007) were divided into four groups, including the second-trimester pregnancy group (n=113), third-trimester pregnancy group (n=110), fetal cerebral VM group (n=12), and healthy control group (n=12) which matched with the VM group in gestational weeks. The amniotic fluid specimens were obtained during amniocentesis or cesarean section. The NGF levels in amniotic fluid were detected with en-zyme-linked immunosorbent assay. Results A significantly negative correlation was found between gestational age and the NGF level in amniotic fluid (r= 0.6149, P<0.0001). The NGF level in patients with fetal VM was significantly lower than that in healthy controls (33.95±29.24 pg/mL vs. 64.73±16.21 pg/mL, P=0.024). Conclusion NGF levels in amniotic fluid may be a sensitive marker for fetal VM.

关键词： nerve growth factor amniotic fluid fetal ventriculomegaly

Dynamic manipulation of multimodal emission in Er^(3+)-activated non-core-shell structure for optical thermometry and information security

在线全文

学校读者我要写书评

暂无评论

Rare Metals 2024年第4期43卷 1702-1712页

作者： Xuan Liu Zi-xia Lin Yin-Biao Shi xiao-yan Wang Ming-Ye Ding xiao-Fei yang College of Science Nanjing Forestry UniversityNanjing210037China Testing Center Yangzhou UniversityYangzhou225009China

Regulating luminescent dynamics of lanthanide-based luminescent materials via external stimuli is of great significance in the fields of optical thermometry and high-level ***,it is still a huge challenge to realize multimodal emissions with tunable color outputs from a single activator in simple structures via smart dynamic control of photon transition ***,we present a mechanistic strategy to achieve multimodal luminescence of Er^(3+)activators with color-switchable outputs in a non-core-shell *** the control of excitation dynamics(λ_(ex)=980,808,1532,377 nm),the population among the intermediate en ergy levels of Er^(3+)and the interaction between Er^(3+)and Yb^(3+)could be precisely modulated through energy transfer and migration processes,leading to the generation of colortunable multimodal luminescence upon diverse excitation modes(non-steady-state,single-/dual-wavelength steady,thermal activation).Inspired by its special luminescent performance,the as-obtained material exhibits great potential in noncontact thermometry,multimodal anticounterfeiting,and high-capacity information encryption by performing a series of proof-of-concept *** findings might provide a conceptual model to modulate the luminescent dynamics in a simple-structured system for the generation of color-adjustable multimodal emissions,which is convenient for the development of advanced luminescent materials toward versatile cuttingedge applications.

关键词： Lanthanide ions Multimodal emission Upconversion Downshifting Anti-counterfeiting Luminescent thermometry

Ginsenoside Rd Induces Differentiation of Myeloid Leukemia Cells via Regulating ERK/GSK-3βSignaling Pathway

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2024年第7期30卷 588-599页

作者： JIANG Yu-xia ZHAO yan-na YU xiao-ling YIN Li-ming Department of Hematology Tongde Hospital of Zhejiang ProvinceHangzhou310012China Institute of Hematology Research the First Affiliated Hospital of Zhejiang Chinese Medical University(Zhejiang Provincial Hospital of Chinese Medicine)Hangzhou310006China

Objective To investigate the role of ginsenoside Rd(GRd)in acute myeloid leukemia(AML)cell *** AML cells were treated with GRd(25,50,100 and 200µg/mL),retinoic acid(RA,0.1g/L)and PD98059(20 mg/mL)for 72 h,cell survival was detected by methylthiazolyldiphenyl-tetrazolium bromide and colony formation assays,and cell cycle was detected by flow *** morphology and differentiation were observed by Wright-Giemsa staining,peroxidase chemical staining and cellular immunochemistry assay,*** protein expression levels of GATA binding protein 1(GATA-1),purine rich Box-1(PU.1),phosphorylated-extracellular signal-related kinase(p-ERK),ERK,phosphorylated-glycogen synthase kinase-3β(p-GSK3β),GSK3βand signal transducer and activator of transcription 1(STAT1)were detected by Western ***-six mice were randomly divided into 3 groups using a random number table:model control group(non-treated),GRd group[treated with 200 mg/(kg·d)GRd]and homoharringtonine(HTT)group[treated with 1 mg/(kg·d)HTT].A tumor-bearing nude mouse model was established,and tumor weight and volume were *** of subcutaneous tumor tissue were observed after hematoxylin and eosin ***1 and GATA-1 expressions were detected by immunohistochemical *** The cell survival was inhibited by GRd in a dose-dependent manner and GRd caused G0/G1 cell arrest(p<0.05).GRd treatment induced leukemia cell differentiation,showing increased expressions of peroxidase and specific proteins concerning erythrogenic or granulocytic differentiation(p<0.05).GRd treatment elicited upregulation of p-ERK,p-GSK-3βand STAT1 expressions in cells,and reversed the effects of PD98059 on inhibiting the expressions of peroxidase,GATA-1 and PU.1(P<0.05).After GRd treatment,tumor weight and volume of mice were decreased,and tumor cells underwent massive apoptosis and necrosis(P<0.05).WT1 level was decreased,and GATA-1 level was significantly increased in subcutaneous tumor ti

关键词： ginsenoside Rd myeloid leukemia survival differentiation extracellular signal-related kinase signaling pathway

Quantum squeezing induced nonreciprocal phonon laser

在线全文

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2024年第6期67卷 20-30页

作者： Tian-xiang Lu yan Wang Keyu xia Xing xiao Le-Man Kuang Hui Jing College of Physics and Electronic Information Gannan Normal UniversityGanzhou 341000China Key Laboratory of Low-Dimensional Quantum Structures and Quantum Control of Ministry of Education Department of Physics and Synergetic Innovation Center for Quantum Effects and ApplicationsHunan Normal UniversityChangsha 410081China Academy for Quantum Science and Technology Zhengzhou University of Light IndustryZhengzhou 450002China College of Engineering and Applied Sciences National Laboratory of Solid State Microstructuresand Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210023China

Phonon lasers or coherent amplifications of mechanical oscillations are powerful tools for fundamental studies on coherent acoustics and hold potential for diverse applications,ranging from ultrasensitive force sensing to phononic information ***,we propose the use of an optomechanical resonator coupled to a nonlinear optical resonator for directional phonon *** find that by pumping the nonlinear optical resonator,directional optical squeezing can occur along the pump *** a result,we can achieve the directional mechanical gain using directional optical squeezing,thereby leading to nonreciprocal phonon lasing with a well-tunable directional power *** work proposes a feasible way to build nonreciprocal phonon lasers with various nonlinear optical media,which are important for a wide range of applications,such as directional acoustic amplifiers,invisible sound sensing or imaging,and one-way phononic networks.

关键词： nonreciprocal phonon laser directional quantum squeezing optomechanics

Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-α release of endothelial cells

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第8期9卷 656-661页

作者： Yi-feng JIANG Feng-di ZHAO xiao-bo LI yan-xia NING Xiu-ling ZHI Rui-zhe QIAN Lian-hua YIN Department of Physiology and Pathophysiology Shanghai Medical College Fudan University Shanghai 200032 China

Objective： To explore the effects of down-regulated tryptase expression in mast cells on the synthesis and release of interleukin-6 （IL-6） and tumor necrosis factor-alpha （TNF-α） of vascular endothelial cells. Methods： Tryptase-siRNA （small-interfering RNA） vector was constructed to inhibit tryptase expression in P815 cells. The medium of P815 cells treated by the tryptase-siRNA （RNAi-P815 group） or pure vector （P815 group） was collected and used to culture bEnd.3 cells. The messenger RNAs （mRNAs） of IL-6 and TNF-α in bEnd.3 cells and their protein levels in the medium were measured by reverse transcription polymerase chain reaction （RT-PCR） and enzyme-linked immunosorbent assay （ELISA）, respectively. Results： IL-6 and TNF-α mRNAs in bEnd.3 cells cultured in RNAi-P815-conditioned medium decreased significantly compared to those in P815-conditioned medium. Consistently, lL-6 and TNF-α protein levels in the medium of bEnd.3 of RNAi-P815 group were lower than those of P815 group. Conclusion： Reduced tryptase expression significantly inhibited the synthesis and release of IL-6 and TNF-α in vascular endothelial cells. RNA interference targeting tryptase expression may be a new anti-inflammatory strategy for vascular diseases.

关键词： Tryptase RNA interference lnterleukin-6 (IL-6) Tumor necrosis factor-alpha (TNF-α) Endothelial cells