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Effects of quercetin on hyper-proliferation of gastric mucosal cells in rats treated with chronic oral ethanol through the reactive oxygen species-nitric oxide pathway

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World Journal of Gastroenterology 2008年第20期14卷 3242-3248页

作者： Jing-Li liu Jun Du Ling-Ling Fan xiao-yan liu Luo Gu Ying-Bin Ge Department of Physiology Nanjing Medical UniversityNanjing 210029 Jiangsu Province China Department of Physiology Henan Universityof Science and Technology Luoyang 471003 Henan ProvinceChina

AIM： To investigate the effect of quercetin （3,3,4,5, 7-pentahydroxy flavone）, a major flavonoid in human diet, on hyper-proliferation of gastric mucosal cells in rats treated with chronic oral ethanol. METHODS： Forty male Sprague-Dawley rats, weighing 200-250 g, were randomly divided into control group （tap wateradlibitum）, ethanol treatment group （6 mL/L ethanol）, quercetin treatment group （intragastric gavage with 100 mg/kg of quercetin per day）, and ethanol plus quercetin treatment group （quercetin and 6 mL/L ethanol）. Expression levels of proliferating cell nuclear antigen （PCNA）and Cyclin D1 were detected by Western blot to assay gastric mucosal cell proliferation in rats. To demonstrate the influence of quercetin on the production of extra-cellular reactive oxygen species/ nitrogen species （ROS/RNS） in rats, changes in levels of thiobarbituric acid reactive substance （TBARS）, protein carbonyl, nitrite and nitrate （NOx） and nitrotyrosine （NT） were determined. The activity of inducible nitric oxide synthase （NOS） including iNOS and nNOS was also detected by Western ***：Compared to control animals, cell proliferation in the gastric mucosa of animals subjected to ethanol treatment for 7 days was significant increased （increased to 290% for PCNA density P 〈 0.05, increased to 150 for Cyclin D1 density P 〈 0.05 and 21.6 ± 0.8 vs 42.3 ± 0.7 for PCNA positive cells per view field）, accompanied by an increase in ROS generation （1.298 ± 0.135 μmol vs 1.772 ± 0.078 μmol for TBARS P 〈 0.05; 4.36 ± 0.39 μmol vs 7.48 ± 0.40 μmol for carbonyl contents P 〈 0.05） and decrease in NO generation （11.334 ± 0.467 μmol vs 7.978 ± 0.334 μmol P 〈 0.01 for NOx; 8.986 ± 1.351 μmol vs 6.854 ± 0.460 μmol for nitrotyrosine P 〈 0.01） and nNOS activity （decreased to 43% P 〈 0.05）. This function was abolished by the co-administration of quercetin. CONCLUSION： The antioxidant action of quercetin relies, in part, on its ability to stimulate nNOS and enhance production of NO that would

关键词： Quercetin Cell proliferation Reactiveoxygen species Nitric oxide Gastric mucosa Ethanol

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Role of remote Coulomb scattering on the hole mobility at cryogenic temperatures in SOI p-MOSFETs

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Chinese Physics B 2020年第3期29卷 473-479页

作者： Xian-Le Zhang Peng-Ying Chang Gang Du xiao-yan liu Institute of Microelectronics Peking UniversityBeijing 100871China

The impacts of remote Coulomb scattering(RCS)on hole mobility in ultra-thin body silicon-on-insulator(UTB SOI)p-MOSFETs at cryogenic temperatures are *** physical models including phonon scattering,surface roughness scattering,and remote Coulomb scatterings are considered,and the results are verified by the experimental results at different temperatures for both bulk(from 300 K to 30 K)and UTB SOI(300 K and 25 K)*** impacts of the interfacial trap charges at both front and bottom interfaces on the hole mobility are mainly evaluated for the UTB SOI p-MOSFETs at liquid helium temperature(4.2 K).The results reveal that as the temperature decreases,the RCS due to the interfacial trap charges plays an important role in the hole mobility.

关键词： remote Coulomb scattering hole mobility cryogenic temperatures UTB SOI p-MOSFETs

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Clinical characteristics and 28-d outcomes of bacterial infections in patients with hepatitis B virus-related acute-on-chronic liver failure

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World Journal of Clinical Cases 2020年第6期8卷 1042-1055页

作者： Chen Li Hai-Bin Su xiao-yan liu Jin-Hua Hu Liver Failure Treatment and Research Center The Fifth Medical Center of Chinese PLA General HospitalBeijing 100039China

BACKGROUND Acute-on-chronic liver failure (ACLF),which includes hepatic and multiple extrahepatic organ failure,is a severe emergency condition that has high *** can rapidly progress and requires an urgent assessment of condition and referral for liver *** infections (BIs) trigger ACLF and play pivotal roles in the deterioration of clinical *** To investigate the clinical characteristics and 28-d outcomes of first Bis either at admission or during hospitalization in patients with hepatitis B virus (HBV)-ACLF as defined by the Chinese Group on the Study of Severe Hepatitis B(COSSH).METHODS A total of 159 patients with HBV-ACLF and 40 patients with acute decompensation of HBV-related chronic liver disease combined with first BIs were selected for a retrospective analysis between October 2014 and March 2016 The characteristics of BIs,the 28-d transplant-free survival rates,and the independent predictors of the 28-d outcomes were *** A total of 194 episodes of BIs occurred in 159 patients with *** the episodes,13.4 To were community-acquired,46.4 To were healthcare-associated,and 40.2% belonged to nosocomial *** (40.7%),spontaneous bacterial peritonitis (SBP)(34.5%),and bloodstream infection (BSI)(13.4%) were the most *** the ACLF grade increased,the incidence of SBP showed a downward trend (P=0.021).Sixty-one strains of bacteria,including 83.6% Gramnegative bacteria and 29.5% multidrug-resistant organisms,were cultivated from 50 patients with *** coli (44.3%) and Klebsiella pneumoniae (23.0%)were the most common *** the ACLF grade increased,the 28-d transplant-free survival rates showed a downward trend (ACLF-1,55.7%;ACLF-2,29.3%;ACLF-3,5.4%;P <0.001).The independent predictors of the 28-doutcomes of patients with HBV-ACLF were COSSH-ACLF score (hazard ratio[HR]=1.371),acute kidney injury (HR=2.187),BSI (HR=2.339),prothrombin activity (HR=0.967),and invasive cathete

关键词： Hepatitis B virus Acute-on-chronic liver failure Bacterial infections Outcomes Chronic liver disease Spontaneous bacterial peritonitis

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Analysis of tumor-infiltrating gamma delta T cells in rectal cancer

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World Journal of Gastroenterology 2016年第13期22卷 3573-3580页

作者： Liang Rong Ke Li Rui Li Hui-Min liu Rui Sun xiao-yan liu Department of Gastroenterology the Fifth Affiliated Hospital of Xinjiang Medical University Department of Gastroenterology the First Affiliated Hospital of Soochow University

AIM: To investigate the regulatory effect of Vδ1 T cells and the antitumor activity of Vδ2 T cells in rectal ***: Peripheral blood, tumor tissues and para-carcinoma tissues from 20 rectal cancer patients were collected. Naïve CD4 T cells from the peripheral blood of rectal cancer patients were purified by negative selection using a Naive CD4+ T Cell Isolation Kit II (Miltenyi Biotec). Tumor tissues and para-carcinoma tissues were minced into small pieces and digested in a triple enzyme mixture containing collagenase type IV, hyaluronidase, and deoxyribonuclease for 2 h at room temperature. After digestion, the cells were washed twice in RPMI1640 and cultured in RPMI1640 containing 10% human serum supplemented with L-glutamine and 2-mercaptoethanol and 1000 U/mL of IL-2 for the generation of T cells. Vδ1 T cells and Vδ2 T cells from tumor tissues and para-carcinoma tissues were expanded by anti-TCR γδ antibodies. The inhibitory effects of Vδ1 T cells on naïve CD4 T cells were analyzed using the CFSE method. The cytotoxicity of Vδ2 T cells on rectal cancer lines was determined by the LDH ***: The percentage of Vδ1 T cells in rectal tumor tissues from rectal cancer patients was significantly increased, and positively correlated with the T stage. The percentage of Vδ2 T cells in rectal tumor tissues from rectal cancer patients was significantly decreased, and negatively correlated with the T stage. After culture for 14 d with 1 μg/mL anti-TCR γδ antibodies, the percentage of Vδ1 T cells from para-carcinoma tissues was 21.45% ± 4.64%, and the percentage of Vδ2 T cells was 38.64% ± 8.05%. After culture for 14 d, the percentage of Vδ1 T cells from rectal cancer tissues was 67.45% ± 11.75% and the percentage of Vδ2 T cells was 8.94% ± 2.85%. Tumor-infiltrating

关键词： Rectal cancer T cells Vδ 1 T cells Vδ 2 T cells Foxp3 Cytotoxicity

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Length Scale of Photospheric Granules in Solar Active Regions

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Research in Astronomy and Astrophysics 2022年第8期22卷 103-110页

作者： yan-xiao liu Chao-Wei Jiang Ding Yuan Ping-Bing Zuo Wen-Da Cao Institute of Space Science and Applied Technology Harbin Institute of TechnologyShenzhen 518055China New Jersey Institute of Technology Center for Solar ResearchNewarkNJUSA Big Bear Solar Observatory 40386 North Shore LaneBig Bear CityCAUSA

Investigating the length scales of granules could help understand the dynamics of granules in the *** this work,we detected and identified granules in an active region near disk center observed at wavelength of TiO(7057?)by the 1.6 m Goode Solar Telescope(GST).By a detailed analysis of the size distribution and flatness of granules,we found a critical size that divides the granules in motions into two regimes:convection and *** length scales of granules with sizes larger than 600 km follow Gauss function and demonstrate“flat”in flatness,which reveal that these granules are dominated by *** with sizes smaller than 600 km follow power-law function and behave power-law tendency in flatness,which indicate that the small granules are dominated by ***,for the granules in active regions,they are originally convective in large length scale,and directly become turbulent once their sizes turn to small,likely below the critical size of 600 *** with the granules in quiet regions,they evolve with the absence of the mixing motions of convection and *** a difference is probably caused by the interaction between fluid motions and strong magnetic fields in active *** strong magnetic fields make high magnetic pressure which creates pressure walls and slows down the evolution of convective *** walls cause convective granules extending to smaller sizes on one hand,and cause wide intergranular lanes on the other *** small granules isolated in such wide intergranular lanes are continually sheared,rotated by strong downflows in surroundings and hereby become turbulent.

关键词： Sun:granulation Sun:photosphere Sun:faculae plages

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Value of gamma-glutamyltranspeptidase-to-platelet ratio in diagnosis of hepatic fibrosis in patients with chronic hepatitis B

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World Journal of Gastroenterology 2017年第41期23卷 7425-7432页

作者： yan-Chao Hu Hao liu xiao-yan liu Li-Na Ma Yu-Hua Guan Xia Luo Xiang-Chun Ding Department of Infectious Diseases General Hospital of Ningxia Medical University Department of Gastroenterology General Hospital of Ningxia Medical University

AIM To investigate the value of the gamma-glutamyltraspeptidase(GGT)-to-platelet(PLT) ratio(GPR) in the diagnosis of hepatic fibrosis in patients with chronic hepatitis B(CHB). METHODS We included 390 untreated CHB patients in this study. The GPR, aspartate aminotransferase(AST)-to-PLT ratio index(APRI), and fibrosis-4(FIB-4) of all patients were analysed to determine if these parameter were correlated with age, gender, medical history, liver function [total bilirubin(TBil), alanine aminotransferase(ALT), and AST], GGT, PLT count, or hepatic fibrosis stage. The GPR, APRI, and FIB-4, as well as the combination of the GPR and APRI or the GPR and FIB-4 were assessed in different cirrhosis stages using receiver operating characteristic(ROC) curve analysis to evaluate their value in diagnosing hepatic fibrosis in CHB patients. RESULTS The GPR, APRI, and FIB-4 were not correlated withCHB patients' age, gender, or disease duration(P > 0.05), but all of these parameters were positively correlated with serum ALT, AST, GGT, and PLT count(P < 0.01). Additionally, the GPR, APRI, and FIB-4 were positively correlated with hepatic fibrosis(P < 0.01); the areas under the ROC curve for the GPR in F1, F2, F3, and F4 stages were 0.723, 0.741, 0.826, and 0.833, respectively, which were significantly higher than the respective values for the FIB-4 and APRI(F1: 0.581, 0.612; F2: 0.706, 0.711; F3: 0.73, 0.751; and F4: 0.799, 0.778). The respective diagnostic cut-off points for each stage were 0.402, 0.448, 0.548, and 0.833, respectively. The diagnostic sensitivity and specificity were, respectively, 88.8% and 87.5% in F1, 72.7% and 89.7% in F2, 81.3% and 98.6% in F3, and 80% and 97.4% in F4 when the GPR and APRI were connected in parallel; 86.6% and 90.2%, 78.4% and 96%, 78.6% and 97.4%, and 73.2% and 97.9%, respectively, when the GPR and APRI were connected in series; 80.2% and 89%, 65% and 89%, 70.3% and 98.5%, and 78.8% and 96.8%, respectively, when the GPR and FIB-4 were connected in

关键词： Gamma-glutamyltraspeptidase-to-platelet ratio APRI FIB-4 Chronic hepatitis B Hepatic fibrosis

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Expression and location of α-fetoprotein during rat colon development

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World Journal of Gastroenterology 2009年第14期15卷 1738-1743页

作者： xiao-yan liu Dan Dong Peng Sun Jun Du Luo Gu Ying-Bin Ge Department of Physiology Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： To investigate the expression of α-fetoprotein （AFP）, a cancer-associated fetal glycoprotein, and its involvement during rat colon development. METHODS： Colons from Sprague-Dawley rat fetuses, young and adult （8 wk old） animals were used in this study. Expression levels of AFP in colons of different development stage were detected by reversetranscriptase PCR （RT-PCR） and Western blotting. To identify the cell location of AFP in the developing rat colons, double-immunofluorescent staining was performed using antibodies to specific cell markers and AFP, respectively. RESULTS： The highest levels of AFP mRNA were detected in colons of rats at embryonic day 18.5 （e18.5）. Compared to e18.5 d, the AFP expression was significantly decreased during rat development （85% for e20.5, P 〈 0.05, 58% for postnatal day 0.5 （P〈0.5）, P 〈 0.05, 37% for P7, P 〈 0.05, 24% for P14, P 〈 0.05, and 11% for P21, P 〈 0.05） and undetected in adult rats. Only the 72-kDa isoform of AFP was detected by Western blotting, the expression pattern was similar to AFP mRNA and conformed to the results of mRNA expression. The AFP positive staining was identical to different distribution patterns in fetuses, young and adult animals and positive staining for both AFP and vimentin was overlapped in mesenchymal cells at each stage tested. CONCLUSION： This study has for the first time demonstrated that AFP is localized in the mesenchyme of rat colon from the embryo to the weaning stage by immunofluorescence and presents 72-kDa isoform in the developing rat colons by Western blotting. The dynamic expression of AFP in the various developmental stages of the colon indicates that AFP might be involved in many aspects of colon development.

关键词： Alpha-fetoprotein Development Mesenchyme Colon Rat

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Orientation controlled photogenerated carriers on selfsupporting CdS/Ni_(3)S_(2) paper toward photocatalytic hydrogen evolution and biomass upgrading

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Rare Metals 2024年第5期43卷 2015-2025页

作者： xiao-yan liu Qing Cao Gui-Xiang Li Hui liu Li-Li Zeng Li-Li Zhao Bin Chang Xi-Wen Wang Hong liu Wei-Jia Zhou Institute for Advanced Interdisciplinary Research School of Chemistry and Chemical EngineeringUniversity of JinanJinan 250022China Institute of Chemical Sciences and Engineering Ecole Polytechnique Federale de Lausanne1015LausanneSwitzerland KAUST Catalysis Center King Abdullah University of Science and TechnologyThuwal 23955-6900Kingdom of Saudi Arabia School of Environment and Energy South China University of TechnologyGuangzhou Higher Education Mega CenterGuangzhou 510006China State Key Laboratory of Crystal Materials Shandong UniversityJinan250100China School of Mechanical Engineering Xi'an Jiaotong UniversityXi'an 710054China

The appropriate regulation of band structure is an effective strategy in constructing efficient photocatalytic *** photocatalytic system mainly employs powder photocatalysts,which makes their recovery reliant on expensive separation processes and severely limits their industrial ***,we constructed a novel CdS/Ni_(3)S_(2)heterostructure using free-standing and flexible nickel fiber paper as the *** regulated energy band structure achieves effective electron–hole *** as-synthesized flexible photocatalyst exhibits considerable photocatalytic activity toward the H_(2)evolution reaction under visible-light irradiation,with an H_(2)production rate of5.63μmol·cm^(-2)·h^(-1)(14.1 mmol·g^(-1)cat·h^(-1)according to the catalyst loading content).Additionally,the otherwisewasted excited holes simultaneously drive organic transformations to yield value-added organic products,thus markedly improving the photocatalytic H_(2)evolution *** a photocatalytic system is scaled up further,where a self-supported 20 cm×25 cm sample achieves a champion H_(2)production rate of 60-80μmol·h^(-1)under practical sun *** newly developed self-supported photocatalyst produces opportunities for practical solar H2production with biomass upgrading.

关键词： Self-supporting photocatalyst CdS/Ni_(3)S_(2) heterojunction Hydrogen evolution Biomass upgrading Large-scaleapplication

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Fertile Woodwardia from the middle Eocene of South China and its implications for palaeogeography and palaeoclimate

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Plant Diversity 2022年第6期44卷 565-576页

作者： Han-Zhang Song Serge V.Naugolnykh Xin-Kai Wu xiao-yan liu Jian-Hua Jin State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Geological Institute of Russian Academy of Sciences Moscow119017Russia Contract Affiliation:Kazan Federal University Republic of TatarstanRussia School of Ecology Sun Yat-sen UniversityGuangzhou 510275China School of Geography South China Normal UniversityGuangzhou 510631China

The genus Woodwardia,which together with the genera Anchistea and Lorinseria comprise the subfamily Woodwardioideae of Blechnaceae,has a disjunct distribution across Central and North America,Europe and the temperate to tropical areas of *** records of Woodwardia occur throughout the Paleogene and Neogene of North America,Europe and ***,well-preserved fertile pinna fossils of this genus have not yet been reported in South *** this paper,a new species,*** Naugolnykh et Song,*** described from the middle Eocene of the Changchang Basin,Hainan Island,South *** and micromorphological features of the fertile pinna show a straight pinna rachis,alternate,subtriangular pinnules,acute pinnule apices,almost entire or slightly undulate pinnule margins,long-ovoid sori,stalked sporangia and spores with wing-like folds on the surface,which are characterised in ***,the present fossil is most similar to the extant species Woodwardia japonica,which mostly grows in warm and moist *** discovery of this new species from the Changchang Basin of Hainan Island indicates that this genus has been distributed in the low-latitude tropical regions of South China from as early as the middle *** on this find,and previous studies of other ferns from the same site,we infer that the climate of the Palaeo-Hainan landscape during deposition of the Changchang Formation was warm and humid,similar to conditions prevailing today across this region.

关键词： Woodwardia Eocene Climate Hainan Island South China

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α-fetoprotein involvement during glucocorticoid-induced precocious maturation in rat colon

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World Journal of Gastroenterology 2011年第24期17卷 2933-2940页

作者： Min Chen Peng Sun xiao-yan liu Dan Dong Jun Du Luo Gu Ying-Bin Ge Lian Yun Gang Higher Vocational Technical College of Traditional Chinese Medicine Lianyungang 222006 Jiangsu Province China Department of Physiology Nanjing Medical University Nanjing 210029 Jiangsu Province China The Laboratory Center for Basic Medical Sciences Nanjing Medical University Nanjing 210029 Jiangsu Province China

AIM： TO investigate the role of a-fetoprotein （AFP）, a cancer-associated fetal glycoprotein, in glucocorticoidinduced precocious maturation in rat colon. METHODS： Colons from suckling Sprague-Dawley rats were used in this study. Corticosterone acetate at a dose of 100 μg/g body weight was given to normal pups on days 7, 9 and 11 after birth to induce hypercorticoidism. Control animals were injected with identical volumes of normal saline. Some rats receiving corticosterone 7 d after birth were also treated with mifepristone （RU38486）, a glucocorticoid cytoplasm receptor antagonist to investigate the effects of glucocorticoids （GCs）. The morphological changes of the crypt depth and villous height of the villous zone in colon were observed as indicesof colon maturation. Expression levels of AFP in colons were detected by reverse transcriptase polymerase chain reaction and Western blotting. To identify the cellular lo- calization of AFP in developing rat colons, double-immu- nofluorescent staining was performed using antibodies to specific mesenchymal cell marker and AFP. RESULTS： Corticosterone increased the crypt depth and villous height in the colon of 8- and 10-d-old rats with hypercorticoidism compared with that in the control ani- mals （120% in 8-d-old rats and 118% in 10-d-old rats in villous height, P = 0.021; 145% in 8-d-old rats and 124% in 10-d-old rats in crypt depth, P = 0.017）. These increases were accompanied by an increase of AFP ex- pression in both mRNA and protein （2.5-folds in 8-d- old and 2.5-folds in 10-d-old rats higher than in control animals, P = 0.035; 1.8-folds in 8-d-old and 1.3-folds in 10-d-old rats higher than in control animals, P = 0.023）. Increased crypt depth and villous height and increased expression of AFP in the colon of rats with hypercorti- coidism were blocked by mifepristone. Both had positive staining for AFP or vimentin, and overlapped in mesen- chymal cells at each tested colon. CONCLUSION： GCs promote the development of ra

关键词： Glucocorticoids a-fetoprotein Precociousmaturation Colon Rat

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