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Limb remote ischemic postconditioning protects integrity of the blood-brain barrier after stroke

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Neural Regeneration Research 2018年第9期13卷 1585-1593页

作者： Juan Li xiao-song hu Fang-Fang Zhou Shuai Li You-Sheng Lin Wen-Qian Qi Cun-Fang Qi xiao Zhang Experiment Technology Center of Preclinical Medicine of Chengdu Medical College Chengdu Sichuan Province China Department of Anatomy Qinghai University Xining Qinghai Province China

Integrity of the blood-brain barrier structure is essential for maintaining the internal environment of the *** of cerebral infarction and brain edema is strongly associated with blood-brain barrier ***,studies have suggested that protecting the blood-brain barrier may be an effective method for treating acute *** examine this possibility,stroke model rats were established by middle cerebral artery occlusion and *** ischemic postconditioning was immediately induced by three cycles of 10-minute ischemia/10-minute reperfusion of bilateral hind limbs at the beginning of middle cerebral artery occlusion *** function of rat models was evaluated using Zea Longa’s *** of the blood-brain barrier was assessed by Evans blue *** volume and brain edema were evaluated using 2,3,5-triphenyltetrazolium chloride *** of matrix metalloproteinase-9 and claudin-5 m RNA was determined by real-time quantitative reverse transcription-polymerase chain *** of matrix metalloproteinase-9 and claudin-5 protein was measured by western blot *** number of matrix metalloproteinase-9-and claudin-5-positive cells was analyzed using *** results showed that remote ischemic postconditioning alleviated disruption of the blood-brain barrier,reduced infarct volume and edema,decreased expression of matrix metalloproteinase-9 m RNA and protein and the number of positive cells,increased expression of claudin-5 m RNA and protein and the number of positive cells,and remarkably improved neurological *** findings confirm that by suppressing expression of matrix metalloproteinase-9 and claudin-5 induced by acute ischemia/reperfusion,remote ischemic postconditioning reduces blood-brain barrier injury,mitigates ischemic injury,and exerts protective effects on the brain.

关键词： nerve regeneration remote ischemic postconditioning middle cerebral artery occlusion cerebral ischemia/reperfusion blood-brain barrier acute cerebral ischemia stroke matrix metalloproteinase-9 claudin-5 neural regeneration

Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells

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World Journal of Gastroenterology 2007年第7期13卷 1129-1134页

作者： Jing Wang xiao-Wen Lv Jie-Ping Shi xiao-song hu Research Center for Eco-Environmental Sciences The Chinese Academy of Sciences Beijing 100085 China Feed Research Institute Chinese Academy ofAgricultural Sciences Beijing 100081 China College of Food Science andNutritional Engineering China Agricultural University BeijingChina

AIM： To investigate the effect of ceramide on the cell cycle in human hepatocarcinoma Bel7402 cells. Possible molecular mechanisms were explored. METHODS： [3- （4, 5）-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide （MTT） assay, plasmid transfection, reporter assay, FACS and Western blotting analyses were employed to investigate the effect and the related molecular mechanisms of C2-ceramide on the cell cycle of Bel7402 cells. RESULTS： C2-ceramide was found to inhibit the growth of Bel7402 cells by indudng cell cycle arrest. During the process, the expression of p21 protein increased, while that of cyclinD1, phospho-ERKl/2 and c-myc decreased. Furthermore, the level of CDK7 was downregulated, while the transcriptional activity of PPARy was upregulated. Addition of GW9662, which is a PPARy specific antagonist, could reserve the modulation action on CDK7. CONCLUSION： Our results support the hypothesis that cell cycle arrest induced by C2-ceramide may be mediated via accumulation of p21 and reduction of cyclinD1 and CDK7, at least partly, through PPARy activation. The ERK signaling pathway was involved in this process.

关键词： Ceramide Cell cycle arrest humanhepatocarcinoma cells P21 CyclinD1 CDK7 PPARy ERK

Electrochemical conversion of methane to bridge the gap in the artificial carbon cycle

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Journal of Energy Chemistry 2025年第1期100卷 286-308页

作者： Yuhao Peng Yuefeng song Ihar Razanau Juanxiu xiao Wei xiao Di hu Guoxiong Wang College of Chemistry and Molecular Sciences Hubei Key Laboratory of Electrochemical Power Sources Wuhan University State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Dalian Institute of Chemical Physics Chinese Academy of Sciences Laboratory of Physical-Chemical Technologies SSPA Scientific and Practical Materials Research Centre of NAS of Belarus State Key Laboratory of Marine Resources Utilization in South China Sea Collaborative Innovation Center of Marine Science and Technology School of Marine Science and Engineering Hainan University Department of Chemical and Environmental Engineering University of Nottingham Ningbo China

Methane, an abundant one-carbon（C1） resource, is extensively used in the industrial production of vital fuels and value-added chemicals. However, current industrial methane conversion technologies are energy-and carbon-intensive, mainly due to the high activation energy required to break the inert C–H bond, low selectivity, and problematic side reactions, including CO2emissions and coke deposition. Electrochemical conversion of methane（ECM） using intermittent renewable energy offers an attractive solution, due to its modular reactor design and operational flexibility across a broad spectrum of temperatures and pressures. This review emphasizes conversion pathways of methane in various reaction systems, highlighting the significance and advantages of ECM in facilitating a sustainable artificial carbon cycle. This work provides a comprehensive overview of conventional methane activation mechanisms and delineates the complete pathways of methane conversion in electrolysis contexts. Based on surface/interface chemistry, this work systematically analyzes proposed reaction pathways and corresponding strategies to enhance ECM efficiency towards various target products, including syngas, hydrocarbons, oxygenates, and advanced carbon materials. The discussion also encompasses opportunities and challenges for the ECM process, including insights into ECM pathways, rational electrocatalyst design, establishment of benchmarking protocols, electrolyte engineering, enhancement of CH4conversion rates, and minimization of CO2emission.

关键词： Methane Electrochemical conversion Reaction mechanism Catalyst design Electrode Electrocatalysis

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不同燃烧室长度的旋转爆震发动机实验研究

推进技术 2016年第10期37卷 1991-2000页

作者：高剑武晓松马虎彭磊徐灿南京理工大学机械工程学院江苏南京210094

为了研究燃烧室长度对旋转爆震发动机(RDE)推力和比冲的影响,设计了RDE推力试验台,以环形燃烧室内径和外径分别为70mm和80mm的RDE作为实验模型,发动机采用环缝-喷孔对撞式掺混方式,以H2和Air分别作为燃料和氧化剂,使用高能火花塞直接点... 详细信息

为了研究燃烧室长度对旋转爆震发动机(RDE)推力和比冲的影响,设计了RDE推力试验台,以环形燃烧室内径和外径分别为70mm和80mm的RDE作为实验模型,发动机采用环缝-喷孔对撞式掺混方式,以H2和Air分别作为燃料和氧化剂,使用高能火花塞直接点火,并用压电式力传感器测量推力,实验研究燃烧室长度分别为40mm,60mm,90mm和120mm的发动机工作性能。通过实验发现,燃烧室长度对RDE稳定工作的工况范围有很大的影响;在稳定工作的工况范围内,燃烧室内的爆震波的传播速度及RDE的推力和比冲随着入口质量流量的增大而逐渐提高,而当量比和燃烧室长度均存在一个最佳值,使爆震波的传播速度及RDE的推力和比冲达到最大值。针对于本论文中的RDE实验模型,基于混合物的比冲最高能够达到74.61s。

关键词：旋转爆震分开喷注燃烧室长度工况范围推力和比冲

Tumor inflammation-specific self-amplifying fluorescent molecular probes induce an anti-tumor immune response

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Science China Chemistry 2025年第1期 273-287页

作者： Shuguang Yang Lijun hu Hong xiao Zecong xiao Xintao Shuai PCFM Lab of Ministry of Education School of Materials Science and Engineering Sun Yat-sen University Department of Ultrasound The Fifth Affiliated Hospital Sun Yat-sen University Department of Medical Ultrasound The Third Affiliated Hospital of Sun Yat-sen University Nanomedicine Research Center The Third Affiliated Hospital of Sun Yat-sen University

Enhancing the concentration of exogenous molecular drugs within the tumor microenvironment through enzyme-catalyzed polymerization presents a novel strategy for cancer therapy. Nonetheless, the optimization of the catalytic efficiency is often impeded by the inefficient expression of enzymes. Herein, we reported a self-amplifying fluorescent molecular probe, Bis-HTPICG, for photodynamic therapy(PDT) and subsequent PDT-induced immunoreaction. The Bis-HTP-ICG probe possesses a noticeable enzyme-catalyzed polymerization facilitated by myeloperoxidase(MPO), a crucial enzyme secreted by neutrophils at inflammation sites. Upon exposure to laser irradiation, Bis-HTP-ICG showed a high PDT efficacy, inducing an acute inflammatory response that stimulates further recruitment of neutrophils and then elevated MPO secretion. The heightened level of MPO enhances the accumulation of the Bis-HTP-ICG via self-polymerization or binding with intratumoral proteins following MPO enzyme catalysis, instigating a self-amplifying chain reaction cycle involving Bis-HTP-ICG, neutrophils and ***, PDT efficiently incites immunogenic cell death(ICD) in tumor cells, initiating an anti-tumor immune response including dendritic cells(DCs) maturation, T cell proliferation and reprogramming of tumor-associated neutrophils(TANs). This work portrays a promising strategy for self-amplification of fluorescent molecular probes through adjustable enzyme levels,potentially offering a unique avenue to enhance the tumor accumulation of molecular drugs for improved tumor therapy.

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Potential role of tanycyte-derived neurogenesis in Alzheimer's disease

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Neural Regeneration Research 2025年第6期20卷 1599-1612页

作者： Guibo Qi Han Tang Jianian hu Siying Kang song Qin Department of Anatomy Histologyand EmbryologySchool of Basic Medical SciencesFudan UniversityShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghaiChina

Tanycytes, specialized ependymal cells located in the hypothalamus, play a crucial role in the generation of new neurons that contribute to the neural circuits responsible for regulating the systemic energy balance. The precise coordination of the gene networks controlling neurogenesis in naive and mature tanycytes is essential for maintaining homeostasis in adulthood. However, our understanding of the molecular mechanisms and signaling pathways that govern the proliferation and differentiation of tanycytes into neurons remains limited. This article aims to review the recent advancements in research into the mechanisms and functions of tanycyte-derived neurogenesis. Studies employing lineage-tracing techniques have revealed that the neurogenesis specifically originating from tanycytes in the hypothalamus has a compensatory role in neuronal loss and helps maintain energy homeostasis during metabolic diseases. Intriguingly,metabolic disorders are considered early biomarkers of Alzheimer's disease. Furthermore,the neurogenic potential of tanycytes and the state of newborn neurons derived from tanycytes heavily depend on the maintenance of mild microenvironments, which may be disrupted in Alzheimer's disease due to the impaired blood–brain barrier ***, the specific alterations and regulatory mechanisms governing tanycyte-derived neurogenesis in Alzheimer's disease remain unclear. Accumulating evidence suggests that tanycyte-derived neurogenesis might be impaired in Alzheimer's disease, exacerbating neurodegeneration. Confirming this hypothesis, however, poses a challenge because of the lack of long-term tracing and nucleus-specific analyses of newborn neurons in the hypothalamus of patients with Alzheimer's disease. Further research into the molecular mechanisms underlying tanycyte-derived neurogenesis holds promise for identifying small molecules capable of restoring tanycyte proliferation in neurodegenerative diseases. This line of investigation could pro

关键词： Alzheimer's disease blood–brain barrier ependymoglial cells hypothalamus metabolic diseases neural stem cells neurogenesis neuroinflammatory diseases neurons tanycyte

Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons

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Neural Regeneration Research 2025年第6期20卷 1816-1824页

作者： Jiantao Cui Yueru Shen Zheng song Dinggang Fan Bing hu Center for Advanced Interdisciplinary Science and Biomedicine of IHM Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina

Rab5 is a GTPase protein that is involved in intracellular membrane trafficking. It functions by binding to various effector proteins and regulating cellular responses, including the formation of transport vesicles and their fusion with the cellular membrane. Rab5 has been reported to play an important role in the development of the zebrafish embryo;however, its role in axonal regeneration in the central nervous system remains unclear. In this study, we established a zebrafish Mauthner cell model of axonal injury using single-cell electroporation and two-photon axotomy techniques. We found that overexpression of Rab5 in single Mauthner cells promoted marked axonal regeneration and increased the number of intra-axonal transport vesicles. In contrast, treatment of zebrafish larvae with the Rab kinase inhibitor CID-1067700markedly inhibited axonal regeneration in Mauthner cells. We also found that Rab5 activated phosphatidylinositol 3-kinase(PI3K) during axonal repair of Mauthner cells and promoted the recovery of zebrafish locomotor function. Additionally, rapamycin, an inhibitor of the mechanistic target of rapamycin downstream of PI3K, markedly hindered axonal regeneration. These findings suggest that Rab5 promotes the axonal regeneration of injured zebrafish Mauthner cells by activating the PI3K signaling pathway.

关键词： axonal regeneration Mauthner cell nerve regeneration Rab5 zebrafish

前路椎弓根螺钉技术用于颈椎前路内固定失败翻修术1例

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中国骨伤 2013年第10期26卷 871-872页

作者：宋小虎徐荣明马维虎宁波市第六医院脊柱外科浙江宁波315040

患者，男，58岁，因“颈椎骨折伴脊髓损伤术后20d，发现内固定松动1d”入院。患者于2011年9月20日因车祸伤致C5、C6椎体骨折伴四肢麻木无力3d收住入院，诊断为C5、C6骨折伴不全瘫，后行C5、C6椎体次全切除钛网植入前路钢板内固定术治疗... 详细信息

患者，男，58岁，因“颈椎骨折伴脊髓损伤术后20d，发现内固定松动1d”入院。患者于2011年9月20日因车祸伤致C5、C6椎体骨折伴四肢麻木无力3d收住入院，诊断为C5、C6骨折伴不全瘫，后行C5、C6椎体次全切除钛网植入前路钢板内固定术治疗。术后患者四肢麻木、无力症状好转出院。

关键词：颈椎骨折骨折固定术 ,内

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沿海滩涂变化监测中沿海边界线变化分析

现代测绘 2020年第4期43卷 31-33页

作者：翟晓彤宋小虎江苏省测绘资料档案馆江苏南京210013 江苏省测绘工程院江苏南京210013

伴随着大规模的围海造地活动,"蓝色土地"成为沿海各地发展新的经济增长点。然而,人类大规模开发近岸陆域带资源的行为已经严重改变了原有的海岸动力系统,致使海岸防线以及海岸生态环境遭受巨大的破坏,海岸灾害隐患进一步加剧,大规模的... 详细信息

伴随着大规模的围海造地活动,"蓝色土地"成为沿海各地发展新的经济增长点。然而,人类大规模开发近岸陆域带资源的行为已经严重改变了原有的海岸动力系统,致使海岸防线以及海岸生态环境遭受巨大的破坏,海岸灾害隐患进一步加剧,大规模的围海造地和港城建设活动给沿海生态环境带来更为巨大的压力。另一方面,合理开发利用滩涂资源已成为国家开发的一项战略措施。通过对江苏省盐城市开展沿海滩涂变化监测试点工作,结合"十五""十一五""十二五"省级基础测绘、第一次地理国情普查、行业专题等多时期、多类型数据,重点对沿海边界线变化进行监测分析,为合理开发利用滩涂资源提供科学依据。

关键词：沿海滩涂边界变化监测