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Decreased expression of serotonin in the jejunum and increased numbers of mast cells in the terminal ileum in patients with irritable bowel syndrome

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World Journal of Gastroenterology 2007年第45期13卷 6041-6047页

作者： Shen-Hao Wang Lei Dong Jin-yan Luo Jun Gong Lu Li xiao-Lan Lu Shui-Ping Han Department of Gastroenterology Second Hospital of Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Department of Pathology Medical School of Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China

AIM: To investigate if there are changes in serotonin (5-HT) levels, enterochromaffin (EC) cells and mast cells in small intestinal mucosa of patients with irritable bowel syndrome (IBS). METHODS: Diarrhea-predominant (IBS-D, n = 20), or constipation-predominant (IBS-C, n = 18) IBS patients and healthy controls (n = 20) underwent colonoscopy and peroral small intestinal endoscopy, and mucosal samples were obtained at the descending part of the duodenum, proximal end of jejunum and terminal ileum. High-performance liquid chromatography- electrochemistry and immunohistochemical methods were used to detect 5-HT content, EC cells and mast cells. RESULTS: (1) There were no differences in the number and distribution of EC cells between IBS patients and the normal group. (2) The mucosal 5-HT contents at the duodenum, jejunum and ileum in IBS-C patients were 182 ± 90, 122 ± 54, 61 ± 35 ng/mg protein, respectively, which were all lower than those in the normal group (256 ± 84, 188 ± 91, and 93 ± 45 ng/ mg protein, respectively), with a significant difference at the jejunum (P < 0.05). There were no differences in the small intestinal mucosal 5-HT contents between IBS-D patients and the normal group. The mucosal 5-HT contents at the duodenum were significantly higher than those at the ileum in the three groups (P < 0.001). (3) The numbers of mast cells in patients with IBS-C and IBS-D at the ileum were 38.7 ± 9.4 and 35.8 ± 5.5/highpower field (hpf), respectively, which were significantly more than that in the normal group (29.8 ± 4.4/hpf) (P < 0.001). There was no significant difference in the numbers of mast cells at the other two parts between IBS patients and the normal group. The numbers of mast cells in IBS-C, IBS-D, and normal groups were all significantly higher at the ileum (38.7 ± 9.4, 35.8 ± 5.5, 29.8 ± 4.4/hpf, respectively) than at the duodenum (19.6 ± 4.7, 18.5 ± 6.3, 19.2 ± 3.3/hpf, respectively, P < 0.001). CONCLUSION: The changes in the 5-HT signaling pathway

关键词： Enterochromaffin cell Irritable bowelsyndrome Mast cell Serotonin Small intestinal mucosa

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MicroRNA-365 Knockdown Prevents Ischemic Neuronal Injury by Activating Oxidation Resistance 1-Mediated Antioxidant Signals

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Neuroscience Bulletin 2019年第5期35卷 815-825页

作者： Jia-Lin Mo Zhi-Guang Pan xiao Chen Yu Lei Ling-Ling Lv Cheng Qian Feng-yan Sun Department of Neurobiology and Institute for Basic Research on Aging and Medicine School of Basic Medical Sciences Fudan University Shanghai 200032 China Department of Neurosurgery National Clinical Research Center for Aging and Medicine Huashan Hospital Shanghai Medical College Fudan University Shanghai 200032 China State Key Laboratory of Medical Neurobiology School of Basic Medical Sciences Fudan University Shanghai 200032 China Shanghai Key Laboratory of Clinical Geriatric Medicine Research Center on Aging and Medicine Shanghai Medical College Fudan University Shanghai 200032 China

Micro RNA-365(mi R-365) is upregulated in the ischemic brain and is involved in oxidative damage in the diabetic rat. However, it is unclear whether mi R-365 regulates oxidative stress(OS)-mediated neuronal damage after ischemia. Here, we used a transient middle cerebral artery occlusion model in rats and the hydrogen peroxide-induced OS model in primary cultured neurons to assess the roles of mi R-365 in neuronal damage. We found that mi R-365 exacerbated ischemic brain injury and OS-induced neuronal damage and was associated with a reduced expression of OXR1(Oxidation Resistance 1). In contrast, mi R-365 antagomir alleviated both the brain injury and OXR1 reduction. Luciferase assays indicated that mi R-365 inhibited OXR1 expression by directly targeting the 30-untranslated region of Oxr1. Furthermore, knockdown of OXR1 abolished the neuroprotective and antioxidant effects of the mi R-365 antagomir. Our results suggest that mi R-365 upregulationincreases oxidative injury by inhibiting OXR1 expression,while its downregulation protects neurons from oxidative death by enhancing OXR1-mediated antioxidant signals.

关键词： MicroRNA Ischemic stroke Neuronal damage Oxidative stress Neuroprotection

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Wind-dispersed seeds blur phylogeographic breaks:The complex evolutionary history of Populus lasiocarpa around the Sichuan Basin

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Plant Diversity 2023年第2期45卷 156-168页

作者： Xue Li Markus Ruhsam Yi Wang Hong-Ying Zhang xiao-yan Fan Lei Zhang Jing Wang Kang-Shan Mao Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610065SichuanPR China Royal Botanic Garden Edinburgh 20A Inverleith RowEdinburgh EH35LRUK

The strength of phylogeographic breaks can vary among species in the same area despite being subject to the same geological and climate history due to differences in biological *** important phylogeographic breaks exist around the Sichuan Basin in Southwest China but few studies have focused on wind-dispersed ***,we investigated the phylogeographic patterns and the evolutionary history of Populus lasiocarpa,a wind-pollinated and wind-dispersed tree species with a circum-Sichuan Basin distribution in southwest *** sequenced and analyzed three plastid DNA fragments(ptDNA) and eight nuclear microsatellites(nSSRs) of 265 individuals of *** from 21 populations spanning the entire distribution *** patterns based on nSSR data revealed that there are three genetic groups in *** is consistent with the three phylogeographic breaks(Sichuan Basin,the Kaiyong Line and the 105°E line),where the Sichuan basin acts as the main barrier to gene flow between western and eastern ***,the distribution pattern based on ptDNA haplotypes poorly matched the phylogeographic breaks,and wind-dispersed seeds may be one of the main contributing *** distribution modelling suggested a larger potential distribution in the last glacial maximum with a severe bottleneck during the last interglacial.A DIYABC model also suggested a population contraction and expansion for both western and eastern *** results indicate that biological traits are likely to affect the evolutionary history of plants,and that nuclear molecular markers,which experience higher levels of gene flow,might be better indicators of phylogeographic breaks.

关键词： Phylogeography Sichuan basin Populus lasiocarpa Kaiyong line Demographic history

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Integrin-linked kinase overexpression promotes epithelial-mesenchymal transition via nuclear factor-κB signaling in colorectal cancer cells

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World Journal of Gastroenterology 2016年第15期22卷 3969-3977页

作者： Hong Shen Jun-Li Ma yan Zhang Gan-Lu Deng yan-Ling Qu xiao-Ling Wu Jing-Xuan He Sai Zhang Shan Zeng Department of Oncology Xiangya Hospital Central South University Institute of Medical Sciences Xiangya Hospital Central South University

AIM: To investigate the effect of integrin-linked kinase (ILK) on proliferation, metastasis, and invasion of the colorectal cancer cell line ***: In this study, the colorectal cancer cell line SW480 was stably transfected with ILK plasmids, and small interfering RNA (siRNA) was used to knockdown expression of nuclear factor (NF)-κB/p65. Methylthiazole tetrazolium (MTT) assay was performed to measure proliferation, and the wound healing migration assay and matrigel invasion assay were used to test the metastasis and invasion ability of SW480 cells. To explore the epithelial-mesenchymal transition (EMT) process, embryonic development, and the invasion and metastasis of tumors, the protein level of E-cadherin, vimentin, snail, and slug was detected by western blot. Immunofluorescence was also used to detect E-cadherin expression. Western blot was used to determine the level of phosphorylated-inhibitor of kappa B (IκB)a, inhibitor of gamma B (IγB)a, and nuclear factor kappa B (NF-κB) expressions and to explore the ILK signaling ***: Western blot results revealed that ILK expression significantly increased when ILK was overexpressed in SW480 cells (P < 0.05). Proliferation, metastasis, and invasion ability were improved in the vector-ILK group compared to the vector group (P < 0.05). Immunofluorescence results revealed that E-cadherin fluorescence intensity decreased after ILK was overexpressed (P < 0.05). Western blot results revealed that the protein expression of E-cadherin was reduced, while vimentin, snail, and slug were upregulated when ILK was overexpressed in SW480 cells (P < 0.05). In order to determine the role of the NF-κB signaling pathway in ILK overexpression promoted EMT occurrence, we overexpressed ILK in SW480 cells and found that levels of NF-κB/p65 and cytoplasmic phosphorylated-IκBa were increased and that cytoplasmic IкBa levels were

关键词： Colorectal cancer Integrin-linked kinase Epithelial-mesenchymal transition Nuclear factor-κ B Overexpression

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基于动态垃圾评价的语音确认方法

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计算机学报 2001年第5期24卷 480-486页

作者： LIU Jun 刘俊 ZHU xiao-yan 朱小燕 State Key Laboratory of Intelligent Technology and Systems Tsinghua University 清华大学计算机科学与技术系北京100084

语音关键词识别和确认方法在语音对话系统中得到了广泛的应用.评价此类系统性能的一个重要指标就是处理非关键词(垃圾)的能力.处理垃圾的传统方法是在离线状态下进行垃圾建模.但该方法并不能很好的描述大量的系统词库以外的词，且训练... 详细信息

语音关键词识别和确认方法在语音对话系统中得到了广泛的应用.评价此类系统性能的一个重要指标就是处理非关键词(垃圾)的能力.处理垃圾的传统方法是在离线状态下进行垃圾建模.但该方法并不能很好的描述大量的系统词库以外的词，且训练规模较大.该文提出了动态垃圾评价方法，不对垃圾本身建模，而是在识别过程中对输入语音进行可信度评估，从而对识别结果进行确认，解决了传统垃圾模型灵活性差及在线垃圾建模方法的确认能力不足等问题.同时由于在垃圾评价中增加了反关键词信息，减少了关键词之间的识别错误.

关键词：语音确认语音识别语音信号处理隐马尔可夫模型动态垃圾评价

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Potential protection of indocyanine green on parathyroid gland function during near-infrared laparoscopic-assisted thyroidectomy: A case report and literature review

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World Journal of Clinical Cases 2020年第21期8卷 5480-5486页

作者： Shu-Jia Peng Ping yang yan-Ming Dong Lin yang Zhen-Yu yang Xi-E Hu Guo-qiang Bao Department of General Surgery Tangdu HospitalAir Force Military Medical UniversityXi’an 710032Shannxi ProvinceChina

BACKGROUND In recent decades,significant advances have been made in protecting the parathyroid glands and recurrent laryngeal nerves during ***,reliable and convenient technical means are still *** this study,the reliability,safety and feasibility of near-infrared(NIR)laparoscopyassisted thyroid lobectomy with isthmectomy and prophylactic central lymph node dissection(CLND)were *** SUMMARY A 63-year-old female patient with a free previous medical history,was admitted to our department due to multiple thyroid *** examination suggested diffuse thyroid changes and one thyroid nodule in the right upper lobe with the largest diameter of 1.5 cm adjacent to the trachea and Breast Imaging Reporting and Data System grade *** examination of the neck showed no obvious enlarged lymph *** needle aspiration biopsy suggested a papillary thyroid *** with thyroid function examination,the patient was diagnosed with papillary thyroid carcinoma and Hashimoto's *** the risk of invading the capsule and the patient's extreme anxiety,a right thyroid lobectomy with isthmectomy and prophylactic CLND was *** significant abnormalities were found during preoperative examinations,except for an increased thyroid stimulating hormone *** patient underwent NIR laparoscopy-assisted thyroid lobectomy with isthmectomy and prophylactic *** the operation,two right parathyroid glands(PGs)adjacent to the thyroid gland capsule and the right recurrent laryngeal nerve(RLN)were examined by indocyanine green(ICG)fluorescence using a NIR fluorescence camera,and the PGs and RLN were reliably *** the ICG-positive PG,prophylactic CLND was *** postoperative parathyroid hormone level was in the normal range and no significant hypocalcemia symptoms were *** During NIR laparoscopy-assisted thyroidectomy,ICG fluorescence may aid PG identification and protecti

关键词： Indocyanine green Near-infrared laparoscopy Carbon nanoparticles Hypoparathyroidism Thyroidectomy Case report

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The penetrating depth analysis of Lunar Penetrating Radar onboard Chang＇e-3 rover

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Research in Astronomy and Astrophysics 2017年第5期17卷 79-90页

作者： Shu-Guo Xing yan Su Jian-Qing Feng Shun Dai Yuan xiao Chun-Yu Ding Chun-Lai Li National Astronomical Observatories Chinese Academy of Sciences Beijing 100012 China University of Chinese Academy of Sciences Beijing 100049 China

Lunar Penetrating Radar（LPR） has successfully been used to acquire a large amount of scientific data during its in-situ detection. The analysis of penetrating depth can help to determine whether the target is within the effective detection range and contribute to distinguishing useful echoes from ***, this study introduces two traditional methods, both based on a radar transmission equation, to calculate the penetrating depth. The only difference between the two methods is that the first method adopts system calibration parameters given in the calibration report and the second one uses high-voltage-off radar data. However, some prior knowledge and assumptions are needed in the radar equation and the accuracy of assumptions will directly influence the final results. Therefore, a new method termed the Correlation Coefficient Method（CCM） is provided in this study, which is only based on radar data without any a priori assumptions. The CCM can obtain the penetrating depth according to the different correlation between reflected echoes and noise. To be exact, there is a strong correlation in the useful reflected echoes and a random correlation in the noise between adjacent data traces. In addition, this method can acquire a variable penetrating depth along the profile of the rover, but only one single depth value can be obtained from traditional methods. Through a simulation, the CCM has been verified as an effective method to obtain penetration depth. The comparisons and analysis of the calculation results of these three methods are also implemented in this study. Finally, results show that the ultimate penetrating depth of Channel 1 and the estimated penetrating depth of Channel 2 range from 136.9 m to 165.5 m（ε_r = 6.6） and from 13.0 m to 17.5 m（ε_r = 2.3）, respectively.

关键词： penetrating Lunar rover lunar assumptions calibration ultimate traces Correlation verified

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Molecular Characterization of Segments S7 to S10 of a Southern Rice Black-streaked Dwarf Virus Isolate from Maize in Northern China

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Virologica Sinica 2011年第1期26卷 47-53页

作者： xiao Yin Fei-fei Xu Fang-qiang Zheng Xiang-dong LI Bao-shen Liu Chun-qing Zhang Shandong Key Laboratory for Agricultural Microbiology College of Plant Protection Shandong Agricultural University Tai＇an Shandong 271018 China College of Agronomy Shandong Agricultural University Tai＇an Shandong 271018 China

Southern rice black-streaked dwarf virus (SRBSDV) is a novel Fijivirus prevalent in rice in southern and central China,and northern Vietnam. Its genome has 10 segments of double-stranded RNA named S1 to S10 according to their size. An isolate of SRBSDV,JNi4,was obtained from naturally infected maize plants from Ji'ning,Shandong province,in the 2008 maize season. Segments S7 to S10 of JNi4 share nucleotide identities of 72.6%-73.1%,72.3%-73%,73.9%-74.5% and 77.3%-79%,respectively,with corresponding segments of Rice black-streaked dwarf virus isolates,and identities of 99.7%,99.1%-99.7%,98.9%-99.5%,and 98.6%-99.2% with those of SRBSDV isolates HN and GD. JNi4 forms a separate branch with GD and HN in the phylogenetic trees constructed with genomic sequences of S7 to S10. These results confirm the proposed taxonomic status of SRBSDV as a distinct species of the genus Fijivirus and indicate that JNi4 is an isolate of SRBSDV. Shandong is so far the northernmost region where SRBSDV is found in China.

关键词： Southern rice black-streaked dwarf virus (SRBSDV) Maize rough dwarf disease (MRD) Identity Phylogenetic analysis

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Enhancement of Phosphorus Solubility by Humic Substances in Ferrosols

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Pedosphere 2008年第4期18卷 533-538页

作者： HUA Quan-Xian LI Jian-Yun ZHOU Jian-Min WANG Huo-yan DU Chang-Wen CHEN xiao-Qin Zhengzhou University Zhengzhou 450001 （China） State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil Science Chinese Academy of Sciences Nanjing 210008 （China） Zhengzhou Institute of Aeronautical Industry Management Zhengzhou 450015 （China）

An investigation was conducted to study the effect of humic substance (HS) on the phosphorus (P) solubility in acidic soil. The soil (2.5 g), HS (0, 0.5, and 2.5 g), and P as monocalcium phosphate (0.31 and 1.25 g P kg-1 soil) were mixed with 50 mL distilled water and two different sequences of adding HS and P were used. The results indicated that the P concentration in water and 0.01 mol L-1 CaCl2 solution increased with increasing amounts of humic substance. The concentrations of Fe and Al were also increased. However, Olsen P decreased with increasing amount of humic substance. Water-soluble P concentrations from P rates at 0.31 and 1.25 g P kg-1 soil in the treatment with 0.5 g (2.5 g) humic substance addition were 360% and 70% (500% and 90%) higher, respectively, than those in the treatment with no humic substance addition. P extracted by 0.01 mol L-1 CaCl2 in the treatments with 0.5 and 2.5 g humic substance addition was increased by 400% and 540%, respectively, compared with that in the treatment without humic substance at the rate of 0.31 g P kg-1 soil, while the corresponding P concentrations were increased by 80% and 90% at the rate of 1.25 g P kg-1 soil. The order of mixing humic substance and phosphate did not significantly affect desorbed P and labile P extracted with CaCl2.

关键词： aluminium Ferrosols humic substance iron phosphorus

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Theoretical predict structure and property of the novel CL-20/2,4-DNI cocrystal by systematic search approach

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Defence Technology（防务技术） 2022年第6期18卷 907-917页

作者： Hao-jie Li Jian-chao Liu Li yang Zhen-zhan yan Yue-wen Lu Ji-min Han xiao-ting Ren Wei Li State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing100081China Science and Technology on Aerospace Chemical Power Laboratory Hubei Institute of Aerospace ChemotechnologyXiangyang441003HubeiChina

Cocrystallization integrates the merits of high energy and insensitivity between energetic molecules to obtain energetics with satisfying ***,how to obtain supramolecular synthons accurately and rapidly for predicting the structure and property of cocrystal remains a challenging *** this research,an efficient systematic search approach to predict CL-20/2,4-DNI cocrystal has been proposed that 2,4-DNI revolves around CL-20 with a stoichiometric ratio of 1:1 in accordance with the specified rules(hydrogen bond length:2.2-3.0 Å;search radius:6.5 Å;the number of hydrogen bond:1-3).Eight possible supramolecular synthons were obtained by combining quantum chemistry with molecular *** structure prediction indicated that there are four structures in cocrystal,namely P21/c,P212121,Pbca and Pna21,and CL-20/2,4-DNI cocrystal is likely to be P21/c and the corresponding cell parameters are Z=4,a=8.28 Å,b=12.17 Å,c=20.42 Å,α=90°,β=96.94°,γ=90°,and ρ=1.9353 g/cm^(3).To further study the intermolecular interaction of CL-20/2,4-DNI cocrystal,a series of theoretical analyses were employed including intermolecular interaction energy,electrostatic potential(ESP),Density of State(DOS),Hirshfeld surface *** C-H…O hydrogen bonds are demonstrated as the predominant driving forces in the cocrystal *** mechanical properties and detonation properties of CL-20/2,4-DNI cocrystal implies that the cocrystal shows better ductility and excellent detonation performances(9257 m/s,39.27 GPa)and can serve as a promising energetic *** structure predicted was compared with the experimental one to verify the accuracy of systematic search *** is a less than 8.8%error between experiment and predict results,indicating the systematic search approach has extremely high reliability and *** systematic search approach can be a new strategy to search supramolecular synthons and identify structures effectively and does have t

关键词： CL-20 2,4-DNI Cocrystal Intermolecular interaction Systematic search

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