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P2X4 receptor participates in autophagy regulation in Parkinson’s disease

Neural Regeneration Research 2021年第12期16卷 2505-2511页

作者： Xue zhang Jing Wang Jin-Zhao Gao xiao-na zhang Kai-Xin Dou Wan-Da Shi An-Mu Xie Department of Neurology Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Neurological Regulation Institute of Qingdao University QingdaoShandong ProvinceChina

Dysfunctional autophagy often occurs during the development of neurodegenerative diseases,such as Parkinson’s disease,Huntington’s disease,and Alzheimer’s *** purinergic P2X4 receptor is an ATP-gated ion channel that is widely expressed in the microglia,astrocytes,and neurons of the central and peripheral nervous systems.P2X4R is involved in the regulation of cellular excitability,synaptic transmission,and ***,the role played by P2X4R in Parkinson’s disease remains poorly *** models of Parkinson’s disease were established by injecting 6-hydroxydopamine into the substantia nigra pars compacta.P2X4R-targeted small interfering Rna(siRna)was injected into the same area 1 week before injury induction to inhibit the expression of the P2X4 *** results showed that the inhibition of P2X4 receptor expression in Parkinson’s disease model rats reduced the rotation behavior induced by apomorphine treatment,increased the latency on the rotarod test,and upregulated the expression of tyrosine hydroxylase,brain-derived neurotrophic factor,LC3-II/LC3-I,Beclin-1,and phosphorylated tropomyosin receptor kinase B(TrkB)in brain tissue,while simultaneously reducing p62 *** findings suggest that P2X4 receptor activation might inhibit neuronal autophagy through the regulation of the brain-derived neurotrophic factor/TrkB signaling pathway,leading to dopaminergic neuron damage in the substantia nigra and the further inhibition of P2X4 receptor-mediated *** results indicate that P2X4 receptor might serve as a potential novel target for the treatment of Parkinson’s *** study was approved by the Animal Ethics Committee of Affiliated Hospital of Qingdao University(approval ***26119)on April 12,2016.

关键词： 6-hydroxydopamine apomorphine brain-derived neurotrophic factor dopaminergic neurons neuron degeneration P2X4R Parkinson’s disease TrkB autophagy

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The mechanism behind activation of the Nod-like receptor family protein 3 inflammasome in Parkinson's disease

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Neural Regeneration Research 2022年第4期17卷 898-904页

作者： Jing Wang xiao-na zhang Jin-Ni Fang Fei-Fei Hua Jing-Yang Han Zeng-Qiang Yuan An-Mu Xie Department of Neurology the Affiliated Hospital of Qingdao UniversityQingdaoShandong ProvinceChina Brain Science Center Academy of Military Medical Sciences of PLABeijingChina

Previous studies have shown that the ATP-P2 X4 receptor signaling pathway mediates the activation of the Nod-like receptor family protein 3(NLRP3)*** NLRP3 inflammasome may promote renal interstitial inflammation in diabetic *** inflammation also plays an important role in the pathogenesis of Parkinson's disease,we hypothesized that the ATP-P2 X4 receptor signaling pathway may activate the NLRP3 inflammasome in Parkinson's disease.A male rat model of Parkinson's disease was induced by stereotactic injection of 6-hydroxydopamine into the pars compacta of the substantia *** P2 X4 receptor and the NLRP3 inflammasome(interleukin-1βand interleukin-18)were *** injection of the selective P2 X4 receptor antagonist 5-(3-bromophenyl)-1,3-dihydro-2 H-benzofuro[3,2-e]-1,4-diazepin-2-one(5-BDBD)or knockdown of P2 X4 receptor expression by si Rna inhibited the activation of the NLRP3 inflammasome and alleviated dopaminergic neurodegeneration and *** results suggest that the ATP-P2 X4 receptor signaling pathway mediates NLRP3 inflammasome activation,dopaminergic neurodegeneration,and dopamine *** findings reveal a novel role of the ATP-P2 X4 axis in the molecular mechanisms underlying Parkinson's disease,thus providing a new target for *** study was approved by the Animal Ethics Committee of Qingdao University,China,on March 5,2015(approval *** 26119).

关键词： ATP neurodegenerative disorder neuroinflammation neuroinflammatory response NLRP3 P2X4 Parkinson's disease

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Development of a test strip for rapid detection of lactoperoxidase in raw milk

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第8期16卷 672-679页

作者： Hong-xia CHE Bo TIAN Li-na BAI Li-ming CHENG Li-li LIU xiao-na zhang Zhan-mei JIANG xiao-xi XU Key Laboratory of Dairy Science (KLDS) Ministry of Education Northeast Agricultural University

Traditional methods for detecting lactoperoxidase （LP） are complex and time-consuming, so a test strip was made based on the enzymatic reaction principle to enable quick and convenient detection of LP in raw milk. In this study 0.1 mol/L citric acid （CA）/0.2 mol/L disodium hydrogen phosphate （naP） buffer solution （pH 5.0）, 22 mmol/L 3,3＇,5,5＇-tetramethylbenzidine （TMB）, 0.6 mmol/L hydrogen peroxide （H202）, and 0.5% Tween-20 or 0.3% cetyltri- methyl ammonium bromide （CTAB） were optimal for preparing a quick, sensitive, and accurate LP test strip. The coefficient of variation （CV） of the estimated LP concentrations ranged from 2.47% to 6.72% and the minimum LP concentration detected by the test strip was 1-2 mg/L. Estimates of active LP in sixteen raw milk samples obtained using the test strip or the TMB method showed a good correlation （c=0.9776）. So the test strip provides a quick, convenient, and accurate method for detecting the LP concentration of raw milk.

关键词： Lactoperoxidase Test strip Rapid detection Raw milk 3 3' 5 5'-Tetramethylbenzidine (TMB)

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Gestational diabetes mellitus combined with fulminant type 1 diabetes mellitus, four cases of double diabetes: A case report

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World Journal of Clinical Cases 2024年第4期12卷 787-794页

作者： Hui Li Yun Chai Wei-Hong Guo Yu-Meng Huang xiao-na zhang Wen-Li Feng Qing He Jin Cui Ming Liu Department of Endocrinology and Metabolism Tianjin Medical University General HospitalTianjin 300052China

BACKGROUND Fulminant type 1 diabetes mellitus(FT1DM)that occurs during pregnancy or the perinatal period is known as pregnancy-related FT1DM(PF),always without history of abnormal glucose ***,we present four patients who developed FT1DM during treatment but were first diagnosed with gestational diabetes mellitus(GDM).CASE SUMMARY The clinical data of four patients with GDM combined with FT1DM admitted to our hospital between July 2018 and April 2021 were collected,and the patients and their infants were followed *** patients were diagnosed with GDM during the second trimester and were *** blood glucose level elevated suddenly during the third trimester and then were diagnosed with *** patients had an insulin allergy,and two had symptoms of upper respiratory tract infection before *** patient developed ketoacidosis,and three developed *** patients had cesarean section deliveries,and two had vaginal *** growth and development of the infants were normal.C-peptide levels were lower than those at onset,suggesting progressive impairment of islet *** frequencies of the DRB109:01,DQB103:03,DQA103:02,DPA101:03,DPA102:02,DPB105:01,DRB401:03,G 01:01,and G 01:04 human leukocyte antigen(HLA)-G alleles were high in the present *** In comparison with pregnancy-associated FT1DM(PF),patients with GDM combined with FT1DM had an older age of onset,higher body mass index,slower onset,fewer prodromal symptoms,and less *** pathogenesis may be due to various factors affecting the already fragileβ-cells of GDM patients with genetically susceptible class II HLA *** speculate that GDM combined with FT1DM during pregnancy,referred to as“double diabetes,”is a subtype of PF with its own unique characteristics that should be investigated further.

关键词： Fulminant type 1 diabetes mellitus Gestational diabetes mellitus Pregnancy-related fulminant type 1 diabetes mellitus Double diabetes Case report

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SPECTRUM OF FUNCTIONING ISLET CELL TUMOR ON MULTISLICE COMPUTED TOMOGRAPHY:EXPERIENCE ON 70 PATIENTS

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Chinese Medical Sciences Journal 2008年第1期23卷 1-9页

作者： Hua-dan Xue Wei Liu Hao Sun Reto Merges Xuan Wang xiao-na zhang Yun Wang Wen-min Zhao Jiu-hong Chen Zheng-yu Jin Department of Radiology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 Medical Solutions Siemens Ltd. Beijing 100102

Objective To review experience in preoperative detection of islet cell tumors using multislice computed tomography （MSCT） and summarize various imaging features of functioning islet cell tumors on enhanced MSCT. Methods Seventy patients with clinical or pathological diagnosis of functioning pancreatic islet cell tumor between October 2003 and February 2007 were included in this retrospective study. Seventy-four enhanced MSCT scans in these patients were identified. All MSCT scans were interpreted by two experienced radiologists by consensus interpretation. Surgery and pathology reports were used to confirm the diagnosis, localization, and size of tumors. Results Totally, 73 functioning islet cell tumors including 65 benign insulinomas, 2 benign glucagonomas, 3 malignant insulinomas, and 3 malignant glucagonomas were pathologically diagnosed. Tumors in only two cases were not found by MSCT. In 67 benign lesions, 32 showed typical enhancement style, 21 showed prolonged enhancement in portal venous phase, 4 showed delayed enhancement, 4 had iso-dense enhancement with normal pancreatic parenchyma, 2 had no enhancement at all in arterial phase and portal venous phase, and 4 had inhomogeneous enhancement with necrosis or cyst-formation. Patchy or spotty calcifications were found in 3 of the 67 tumors. In 6 malignant islet cell tumors, vessel invasion （2/6） and bowel invasion （1/6） were seen. Different enhancement patterns were shown. All hepatic metastases showed hyper-enhancement during their arterial phase. Conelttsions Pancreatic islet cell tumor may display a wide spectrum of presentations in MSCT. Tumors with unusual appearances often present as diagnostic challenges. Non-contrast and post-contrast multiphase scans are recommended for the localization of functioning islet cell tumors.

关键词： islet cell tumor pancreas multislice computed tomography

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Amyloid β3-10 Dna vaccination suggests a potential new treatment for Alzheimer＇s disease in BALB/c mice

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Chinese Medical Journal 2011年第17期124卷 2636-2641页

作者： XING xiao-na zhang Wei-ge SHA Sha LI Yu GUO Rong WANG Cai CAO Yun-peng Department of Neurology First Affiliated Hospital of China Medical University Shenyang Liaoning 110001 China

Background Amyloid β1-42 （Aβ42） peptide vaccination has been proved to be effective in reducing amyloid burden in brain and improving cognitive function in Alzheimer＇s disease （AD） mouse models. But the phase II trial of Aβ42 peptide vaccine was halted because of T cell-mediated meningoencephalitis. In this study, a Dna vaccine, p（Aβ3-10）10-CpG, was constructed to test whether it would induce predominant TH2 immune response upon immunization of BALB/c mice. Methods BALB/c mice were vaccinated intramuscularly with p（Aβ3-10）10-CpG plasmids. Aβ42 peptide, pcDna3.1（＋） empty vector and PBS were injected to the control groups. Expression of interesting gene in injected muscle was identified by immunohistochemistry. Anti-Aβ antibody titers, isotype profiles as well as cytokines in ex vivo splenocytes culture supernatants were analyzed by enzyme-linked immunosorbent assay （ELISA）. Results P（Aβ3-10）lo-CpG plasmid was expressed in muscle after injection detected by immunohistochemistry. The p（Aβ3-10）10-CpG vaccine induced high titers of anti-Aβ antibodies in BALB/c mice. And isotype of the antibodies was mainly IgG1, the IgG1/IgG2a ratio for the p（Aβ3-10）10-CpG group was approximately 5 times greater than that for the Aβ42 peptide group. Ex vivo cultured splenocytes isolated from mice immunized with p（Aβ3-10）10-CpG exhibited high interleukin-4 response and low interleukin-y （IFN-γ） response. Conclusions Immunization with p（Aβ3-10）10-CpG vaccine primarily induces a TH2 type of response, thus reduces the probability of inflammation. This （Aβ3-10）10-CpG vaccine possesses the basic factors required for a safe and effective AD vaccine.

关键词： Alzheimer's disease vaccine Dna

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阿尔茨海默病的全程管理

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中华老年医学杂志 2017年第4期36卷 355-357页

作者：肖卫忠张娜北京大学第三医院神经内科 100191 北京大学第三医院康复科 100191

阿尔茨海默病（AD）是老年人常见的神经变性疾病，以逐渐进展的记忆障碍、全面智能减退、个性改变以及精神行为异常为主要临床表现，通常病情呈进行性加重，逐渐丧失独立生活能力。AD是老年痴呆最常见的类型，其发病率随年龄而逐渐增高... 详细信息

阿尔茨海默病（AD）是老年人常见的神经变性疾病，以逐渐进展的记忆障碍、全面智能减退、个性改变以及精神行为异常为主要临床表现，通常病情呈进行性加重，逐渐丧失独立生活能力。AD是老年痴呆最常见的类型，其发病率随年龄而逐渐增高，是继心脑血管病和肿瘤之后，威胁老年人健康的重要原因。AD是持续进展性疾病，需要全程综合管理。

关键词：阿尔茨海默病疾病管理

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Assessment of Coronary Stents by 64-slice Computed Tomography: In-stent Lumen Visibility and Patency

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Chinese Medical Sciences Journal 2009年第3期24卷 156-160页

作者： Ling-yan Kong Zheng-yu Jin Shu-yang zhang Zhu-hua zhang Yi-ning Wang Lan Song xiao-na zhang Yun-qing zhang Department of Radiology Department of Cardiology Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To assess lumen visibility of coronary stents by 64-slice computed tomography （CT） coronary angiography, and determine the value of 64-slice CT in non-invasive detecting of in-stent restenosis after coronary artery stent implantation. Methods Totally, 60 patients （54 males, aged 57.0±12.7 years） and /05 stents were investigated by 64-slice CT at a mean interval of 20.0±16.6 months after coronary stents implantation. Axial multi-planar reconstruction images of the stents and curved-planar reconstruction images through the median of the stents were reconstructed for evaluating stent image quality on a 5-point scale （1=excellent, 5=nonassessable）, and stent lumen diameter was detected. Conventional coronary angiography was performed in 18 patients, and 32 stents were evaluated. Results Image quality was good to excellent on average （score 1.71±0.76）. Stent image quality score was correlated to heart rate （r=0. 281, P〈0.01） and stent diameter （r=-0.480, P〈0.001）. All the stents were assessable in lumen visibility with an average visible lumen diameter percentage of 60.7%±13.6%. Visible lumen diameter percentage was correlated to heart rate （r=-0.193, P〈0.05）, stent diameter （r=0.403, P〈0.001）, and stent image quality score （r=-0.500, P〈0.001）. Visible lumen diameter percentage also varied depending on the stent type. In comparison with the conventional coronary angiography, 4 of 6 in-stent stenoses were correctly detected. The sensitivity and specificity for the detection of in-stent stenosis were 66.7% and 84.6%, respectively. Conclusions Using a 64-slice CT, the stent lumen is partly visible in most of the stents. And 64-slice CT may be useful in the assessment of stent patency.

关键词： coronary artery in-stent restenosis computed tomography

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Effect of Zangfu ointment and massage therapy on functional constipation:a protocol for a randomised controlled trial

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Gastroenterology & Hepatology Research 2023年第2期5卷 16-20页

作者： xiao-na zhang nai-Wei zhang Li-Bao An Bao-Tuan Zhao Wei-Fang Liu Bo-Yi Jia Hong-Jie Cheng Digestive Department Fangshan HospitalBeijing University of Chinese MedicineBeijing 102400China

Introduction:Functional Constipation(FC)is a type of functional bowel disease that is in Clinically characterized by dysportia,decreased frequency of bowel movements,or incomplete bowel movements in the absence of irritable bowel syndrome syndrome,at least 6 months before diagnosis,and symptoms within the last 3 *** present,commonly used drugs include enema and suppositories,laxatives,microecological preparations,gastrointestinal motility drugs and other ***,the effect is *** Chinese medicine(TCM)treatment of FC has ***:In this randomized controlled study,244 eligible patients were randomly assigned in a 1:1 ratio to a treatment group(Zangfu ointment and massage therapy+Lactulose Oral Liquid)and a control group(Lactulose Oral Liquid)for 14 *** of spontaneous defecation per week and First defecation time will be used as primary outcomes,and Traditional Chinese Medicine(TCM)syndrome scores and syndrome scores,PAC-QOL form will be used as secondary *** routine,liver function,and renal function will be used as safety *** primary and secondary outcomes will be performed at 0th,7th,and 14th day,and the safety outcomes will be performed at 0th and 14th *** and dissemination:Ethical approval has been obtained from the Committee on Health Research Ethics of the Fangshan Hospital,Beijing University of Chinese Medicine,Beijing,People's Republic of China(FZY LK-2020-010)on September 18,*** patients will be provided oral and written information about the trial before *** study results will be disseminated by peer-review publications and conference *** registration number:ChiCTR2000038754(registered on April 14,2021).This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial license,which permits others to distribute,remix,adapt,build upon this work non-commercially,and license their derivative works on different

关键词： functional constipation Zangfu ointment and massage therapy Qi stagnation a randomized controlled clinical trial

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肿瘤坏死因子-αG-308A多态性与自发性早产遗传易感性的关联研究

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中国当代儿科杂志 2016年第12期18卷 1247-1253页

作者：彭薇杨晓朱丽娜张小爱王艳中国人民解放军陆军总医院附属八一儿童医院北京100700 军事医学科学院微生物和流行病学研究所病原微生物生物安全重点实验室北京100071

目的探讨肿瘤坏死因子-α（TNF-α）G-308A多态性和自发性早产（SPTB）易感性的关联。方法收集753例SPTB患儿为病例组,681例足月新生儿为对照组,利用Sequenom Mass ARRAY~？SNP检测技术对TNF-α基因G-308A多态性位点进行单核苷酸多态性... 详细信息

目的探讨肿瘤坏死因子-α（TNF-α）G-308A多态性和自发性早产（SPTB）易感性的关联。方法收集753例SPTB患儿为病例组,681例足月新生儿为对照组,利用Sequenom Mass ARRAY~？SNP检测技术对TNF-α基因G-308A多态性位点进行单核苷酸多态性检测。结果病例组和对照组等位基因G、A分布频率之间的差异无统计学意义（P=0.35）;GG、GA、AA基因型构成比之间的差异也无统计学意义（P=0.64）。Logistic回归分析发现TNF-α基因G-308A位点与SPTB的遗传易感性不相关（OR=0.85,95%CI：0.61~1.19,P=0.35）。结论 TNF-α基因G-308A多态性位点与SPTB遗传易感性不相关。

关键词：肿瘤坏死因子-α 自发性早产单核苷酸多态性早产儿

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