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Evodiamine inhibits high-fat diet-induced colitis-associated cancer in mice through regulating the gut microbiota

Journal of Integrative Medicine 2021年第1期19卷 56-65页

作者： Li-qing Zhu Li Zhang Jia Zhang Guo-lin Chang Gang Liu Dan-dan yu xiao-min yu Mi-sheng Zhao Bin Ye Department of Pathogenic Biology Chongqing Medical UniversityChongqing 400016China Research Center for Molecular Medicine and TumorChongqing Medical UniversityChongqing 400016China Department of Clinical LaboratoryThe First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325023Zhejiang ProvinceChina Department of PathophysiologyChongqing Medical UniversityChongqing 400016China Department of Clinical LaboratoryWenzhou People’s HospitalWenzhou 325023Zhejiang ProvinceChina Department of EmergencyUniversity-Town Hospital of Chongqing Medical UniversityChongqing 401331China

Objective:High-fat diet is one of the main risk factors that disrupt the balance of gut microbiota,which eventually will induce colorectal cancer(CRC).Evodiamine(EVO)is a wildly used multifunctional traditional Chinese medicine *** this study,we investigated the role of gut microbiota in high-fat dietpropelled CRC and the potential of EVO for CRC ***:Gut microbiota,serum D-lactic acid and endotoxin from 38 patients with colon cancer and 18 healthy subjects were detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA).In addition,body mass index,phospho-signal transducer and activator of transcription 3(p-STAT3)expression in cancer tissues and paracancerous tissues were detected by immunohistochemistry.A mouse intestinal inflammatory tumor model was established by azomethane/sodium dextran sulfate,followed by treatment with EVO and 5-aminosalicylic acid(ASA).Gut microbiota and inflammatory factors were detected by quantitative polymerase chain reaction,while serum D-lactic acid and endotoxin were detected by ***,cell proliferation,cell apoptosis,and interleukin(IL)-6/STAT3/P65 pathway were evaluated by 5-ethynyl-20-deoxyuridine,terminal-deoxynucleotidyl transferase-mediated nick-end labeling,and Western blot ***:In patients with colon cancer,the numbers of Enterococcus faecalis and Escherichia coli were increased,while those of Bifidobacterium,Campylobacter and Lactobacillus were *** endotoxin and D-lactic acid levels and p-STAT3 levels were significantly *** the mouse model,both EVO and ASA inhibited tumor formation,decreased the proliferation of tumor cells,and induced apoptosis of tumor *** with the control group,the numbers of *** and *** were decreased,while Bifidobacterium,Campylobacter and Lactobacillus numbers were *** the EVO group,serum endotoxin and D-lactic acid levels and inflammatory factors were signifi

关键词： Evodiamine Gut microbiota Colitis-associated cancer Signal transducer and activator of transcription 3

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The use of melittin to enhance transgene expression mediated by recombinant adeno-associated virus serotype 2 vectors both in vitro and in vivo

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Journal of Integrative Medicine 2023年第1期21卷 106-116页

作者： Yi-lin Xie Ji-yao Wang yun He xiao-min yu Qing-yun Zheng Chen Ling Xi-lin Feng Li-qing Zhu State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(ministry of Education) School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Department of Clinical Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province Wenzhou 325000Zhejiang ProvinceChina Yantai Fuheng Biological Technology Co. Ltd.Yantai 264006Shandong ProvinceChin

Objective: Melittin, a cell-penetrating peptide, improves the efficiency of many non-viral gene delivery vectors, yet its application in viral vectors has not been well studied. The non-pathogenic recombinant adeno-associated virus(rAAV) vector is an ideal in vivo gene delivery vector. However, its full potential will only be achieved after improvement of its transduction efficiency. To improve the transduction efficiency of rAAV2 vectors, we attempted to develop a melittin-based r AAV2 vector delivery ***: The melittin peptide was inserted into the rAAV2 capsid either in the loop Ⅷ of all viral proteins(VPs) or at the N terminus of VP2. Various r AAV2-gfp or-fluc vectors were subjected to quantitative real-time polymerase chain reaction and Western blot assays to determine their titers and integrity of capsid proteins, respectively. Alternatively, the vectors based on wild-type capsid were pre-incubated with melittin, followed by transduction of cultured cells or tail vein administration of the mixture to C57BL/6 and BALB/c nude mice. In vivo bioluminescence imaging was performed to evaluate the transgene ***: rAAV2 vectors with melittin peptide inserted in the loop Ⅷ of VPs had low transduction efficiency, probably due to dramatically reduced ability to bind to the target cells. Fusing the melittin peptide at the N-terminus of VP2 produced vectors without the VP2 subunit. Interestingly, among the commonly used rAAV vectors, pre-incubation of r AAV2 and rAAV6 vectors with melittin significantly enhanced their transduction efficiency in HEK293 and Huh7 cells in vitro. Melittin also had the ability to increase the rAAV2-mediated transgene expression in mouse liver in vivo. Mechanistically, melittin did not change the vector-receptor interaction. Moreover, cell counting kit-8 assays of cultured cells and serum transaminase levels indicated melittin had little ***: Pre-incubation with melittin, but not insertion of melitt

关键词： TAAV Melittin Capsid engineering Co-administration Transduction efficiency

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Melittin analog p5RHH enhances recombinant adeno-associated virus transduction efficiency

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Journal of Integrative Medicine 2024年第1期22卷 72-82页

作者： Jing-shun Meng yun He Heng-bin Yang Li-ping Zhou Si-yuan Wang Xi-lin Feng Omar Yahya Al-shargi xiao-min yu Li-qing Zhu Chang-quan Ling Shanghai University of Traditional Chinese Medicine Shanghai 201203China Oncology Department of Traditional Chinese Medicine Changhai HospitalNaval Medical UniversityShanghai 200433China Faculty of Traditional Chinese Medicine Naval Medical UniversityShanghai 200043China State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(ministry of Education) School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Yantai Fuheng Biological Technology Co. Ltd.YantaiShandong Province 264006China. Department of Clinical Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina

Objective Melittin and its derivatives have been characterized to establish effective gene delivery *** capability of facilitating endosomal release enhances the nanoparticles-based gene ***,little investigation has been conducted to explore its potential application in the context of viral *** Various melittin-derived peptides were inserted into the loop VIII of the capsid proteins of recombinant adeno-associated virus *** vectors carrying either gfp or fluc genes were subjected to qPCR assays and transduction assays of HEK293T cells to investigate the efficiency of vector production and gene *** addition,the ability of a specific p5RHH-rAAV vector to deliver genes was examined through in vitro transduction of different cultured cells and in vivo tail vein administration to C57BL/6 ***,the intricate details of the vector-mediated transduction mechanisms were revealed by specific pharmacological inhibitors of every stage of the rAAV2 intracellular life *** A total of 76 melittin-related peptides were compiled from existing *** them,cMA2,Melt13,p5RHH and aAR3 were found to significantly enhance the gene delivery efficiency of rAAV2 *** p5RHH-rAAV2 vectors efficiently transduced not only rAAV-potent cell lines but also cell lines previously considered resistant to ***,bafilomycin A1,a vacuolar endosome acidification inhibitor,completely inhibited the transgene expression mediated by the p5RHH-rAAV2 *** importantly,p5RHH-rAAV8 vectors also demonstrated increased hepatic transduction in vivo in C57BL/6 *** The incorporation of melittin analogues into the rAAV capsids results in a significant improvement in rAAV-mediated transgene *** further modifications remain an area of interest,our studies have substantially broadened the pharmacological prospects of melittin in the context of viral vector-mediated gene delivery.

关键词： Melittin Recombinant adeno-associated virus Capsid engineering Transduction efficiency

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城市交通满意度定量化评价方法探讨

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重庆交通大学学报（自然科学版） 2009年第1期28卷 111-115页

作者：肖裕民重庆交通大学交通运输学院重庆400074

通过对一些城市交通状况的多种统计数据的调查分析,从大气和噪声污染、能源的无用损耗及道路通行状况等影响交通满意度的3个主要因素进行分析,建立了一套测试居民对城市交通满意度的数学模型。通过对城市道路密度、机动车数量和对环境... 详细信息

通过对一些城市交通状况的多种统计数据的调查分析,从大气和噪声污染、能源的无用损耗及道路通行状况等影响交通满意度的3个主要因素进行分析,建立了一套测试居民对城市交通满意度的数学模型。通过对城市道路密度、机动车数量和对环境的影响因子的定量化描述,给出了各种因素在模型中所占有的权值。为测试该模型最终量化数据能够真实、有效的反应各地居民对当地交通状况的满意程度,文章最后给出了各地满意度计算结果与实际原因的可能性分析,从而验证了本模型的正确性。

关键词：交通满意度交通环境容量驾车人满意度居民的满意度

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Late-stage Use of Low-dose Corticosteroids Aid Recovery of Severe H1N1 Viral Pneumonia

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国际感染病学（电子版） 2013年第3期2卷 132-136页

作者： Bin Cao Li Gu xiao-min yu yu-Dong Yin Chen Ma Ying-mei Liu Department of Infectious Diseases and Clinical Microbiology Beijing Chao-Yang Hospital (Beijing Institute of Respiratory Medicine)Capital Medical University

The role of corticosteroids in the management of severely ill patients with inlfuenza A (H1N1) viral infection is unclear and controversial. Two critically ill cases with influenza A (H1N1) infections complicated with organizing pneumonia (OP) in 2011 successfully treated with low dose corticosteroids were reported here. After initial clinical improvement, the condition of both patients aggravated 20-23 days after the onset of illness. Chest X-ray and computed tomographies (CT) showed an increment of lung infiltrates. Cultures of blood, pleural lfuid and transbronchial aspirate were negative for bacteria and fungi. Organizing pneumonia was diagnosed clinically and both patients were successfully treated with low-dose corticosteroids. Low-dose corticosteroids initiated during convalescence may be beneficial for severe swine-origin influenza A H1N1 pandemic 2009 virus (S-OIV) infections.

关键词： H1N1 Pneumonia Corticosteroids

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外源钙缓冲作用的动力学模型

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系统仿真学报 2008年第22期20卷 6083-6087页

作者：施小民郑毓蕃上海大学系统科学研究所上海200444

外源钙缓冲具有调控细胞内钙信号的功能。最近的实验表明慢速和快速外源钙缓冲的作用均能降低胞质钙振荡的振幅和钙瞬变的衰减率。将外源钙缓冲的影响导入至Atri等人的模型中,分别得到了慢速钙缓冲作用的三变量模型和快速钙缓冲作用的... 详细信息

外源钙缓冲具有调控细胞内钙信号的功能。最近的实验表明慢速和快速外源钙缓冲的作用均能降低胞质钙振荡的振幅和钙瞬变的衰减率。将外源钙缓冲的影响导入至Atri等人的模型中,分别得到了慢速钙缓冲作用的三变量模型和快速钙缓冲作用的二变量模型。模型表明外源钙缓冲物的作用,不仅降低了钙信号的振幅和衰减率,还降低了周期振荡的频率。并且,模型预测存在一个钙缓冲物浓度的最小值,可以消除周期振荡。

关键词：钙振荡钙缓冲钙信号 Hopf分岔

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一种新的公交乘客上车站点确定方法

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重庆交通大学学报（自然科学版） 2009年第1期28卷 121-125页

作者：于勇邓天民肖裕民重庆交通大学交通运输学院重庆400074 重庆大学自动化学院重庆400044

公交IC卡是一种新的客流信息采集手段,它的广泛使用为公交管理者进行客流分析提供了新的数据源。充分利用公交IC卡刷卡时间信息,根据相邻两站点间的最短刷卡间隔,通过系统聚类法对IC卡刷卡数据按照时间顺序进行聚类分析。在此基础上,将... 详细信息

公交IC卡是一种新的客流信息采集手段,它的广泛使用为公交管理者进行客流分析提供了新的数据源。充分利用公交IC卡刷卡时间信息,根据相邻两站点间的最短刷卡间隔,通过系统聚类法对IC卡刷卡数据按照时间顺序进行聚类分析。在此基础上,将聚类分析的结果与公交运营资料所推算出的车辆到站时间进行时间匹配,确定持卡乘客的上车站点。最后,通过实例验证了该方法的可行性与实用性。

关键词：公交IC卡信息系统聚类法 BUS到站时刻预测

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混凝土路面加铺层界面端奇异场分析和加铺层材料比选

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重庆交通大学学报（自然科学版） 2011年第1期30卷 39-43页

作者：宁金成肖裕民河南交通职业技术学院河南郑州450005 重庆交通大学交通运输学院重庆400074

水泥混凝土路面采用水泥混凝土加铺层或者沥青加铺层均会形成界面端。界面端的结合界面存在应力奇异性,这与加铺层材料性质和其下原路面的材料性质相关,通过对界面端的应力场分析表明:界面端的奇异性指数和应力场的场参量具有良好的相关... 详细信息

水泥混凝土路面采用水泥混凝土加铺层或者沥青加铺层均会形成界面端。界面端的结合界面存在应力奇异性,这与加铺层材料性质和其下原路面的材料性质相关,通过对界面端的应力场分析表明:界面端的奇异性指数和应力场的场参量具有良好的相关性,能够较好的反映加铺层界面端的应力奇异程度。因此在实践中可直接采用界面端奇异性指数进行加铺层材料选材,从而避免复杂的场分析。基于界面端场分析的结论,根据界面端场奇异性指数给出了水泥混凝土路面加铺层材料的选材原则:加铺层材料性质和原路面材料性质接近,可降低界面端的应力奇异程度,从而使路面加铺端的受力更加有利。

关键词：水泥混凝土路面加铺层界面端应力奇异性加铺层材料优选

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Synergistically enhanced ORR and HER performance on Co-N-C coupled in-situ generated PtCo intermetallic

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Journal of Energy Chemistry 2025年第1期100卷 721-729页

作者： Guanyu Luo min Song Lulu An xiao Huang Qian Zhang Chenhao Zhang Tao Shen Shuang Wang Deli Wang Key Laboratory of Material Chemistry for Energy Conversion and Storage (Huazhong University of Science and Technology) ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology

Integrating multi-scale sites in a composite catalyst is vital to realize efficient ***,a synergistic composite catalyst consisting of Co atomic sites and in-situ generated PtCo intermetallic compounds （IMCs）(o-PtCo@CoNC) is proposed through Co pre-anchoring and subsequent impregnation-reduction *** loading of Co atoms provides a chance for in-situ generating PtCo ordered intermetallic *** remaining Co single atoms and PtCo IMCs construct synergistic electrocatalytic *** from the ordered structure,synergistic effect of Pt Co IMCs and Co single atoms,o-PtCo@CoNC exhibits excellent electrocatalytic performance for oxygen reduction reaction （ORR） and hydrogen evolution reaction （HER） with mass activity of 1.21 A mgPt-1（at 0.9 V） and5.70 A mgPt-1（at an overpotential of 100 mV）,***,o-PtCo@CoNC delivers negligible loss of half-wave potential and overpotential during long-term stability test in acid solutions,with 13 mV decay after 50,000 potential cycles for ORR and a 2.7 mV decay after 20,000 potential cycles for *** integration strategy of single-atomic sites coupled IMCs paves the way for enhancing the activity and durability of Pt-based electrocatalysts.

关键词： Electrolytic reduction

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Observation of acoustic hybrid topological phases induced by the p-d orbital interactions

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 132-139页

作者： xiao Xiang Feng Gao yu-Gui Peng Peng Wu Zong-Lin Li Xue-Feng Zhu School of Physics and Innovation Institute Huazhong University of Science and Technology

In spinless systems,growing attention has recently been attracted to synthetic gauge fields,which redefine the fundamental crystal symmetries by utilizing the projective algebraic ***,synthetic gauge fields have been commonly investigated in single orbital systems,and explorations on hybrid orbitals remain elusive in ***,we report the experimental observation of hybrid topological phases induced by hybrid synthetic gauge flux,which is formed by the interaction between p and d orbitals embedded in acoustic *** breaking the translation symmetries of Lxand/or Ly,we unambiguously demonstrate the first-order M?bius edge states and higher-order corner *** work establishes a platform for seeking exotic topological phases induced by hybrid-orbitals interactions and initializing the framework of hybrid-orbitalsrelated topological *** applications can be anticipated in scenarios such as high-quality sensing and robust wave trapping due to the stepwise energy distribution of the hybrid topological phase.

关键词： topological insulator synthetic gauge field projective symmetry corner states

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