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Novel Role of ER Stress and Mitochondria Stress in Serum-deprivation Induced Apoptosis of Rat Mesenchymal Stem Cells

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Current Medical Science 2018年第2期38卷 229-235页

作者： Tong QIU Yan-yan HE xiao ZHANG xiao-lin ma State Key Laboratory of Advanced Technology for materials Synthesis and Processing Biomedical Material and Engineering Center Wuhan University of Technology Wuhan 430070 China School of materials Science and Engineering Wuhan University of Technology Wuhan 430070 China Biomedical materials and Engineering Research Center of Hubei Province Wuhan University of Technology Wuhan 430070 China International School of materials Science and Engineering Wuhan University of Techonology Wuhan 430070 China Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients Hubei University of Education Wuhan 430205 China

The poor survival of mesenchymal stem cells （MSCs） compromises the efficacy of stem cell therapy. Growth factor deprivation is one of the important factors that have challenged the survival of donor MSCs in cell therapy. In this study, the aim was to evaluate the effect of serum deprivation on the cell death of MSCs and to investigate the underlying mechanisms. Apoptosis of MSCs was evaluated with Hoechst 33342/PI staining. Signaling pathways involved in serumdeprivation induced apoptosis were analyzed using Western blotting. The results revealed that serum deprivation induced apoptosis in MSCs within 72 h of treatment. Serum deprivation was shown to lead to protein expression alterations in Bax, Bcl-2, casepase-3, casepase-8, GRP78, and CHOP during experiments. The data suggested that the mitochondria death pathway, the extrinsic apoptotic pathway and the endoplastic reticulum（ER） stress pathway were all involved in MSCs apoptosis. The increase in expression of CHOP and the simultaneous decrease in Bcl- 2 expression suggest a synergistic effect in apoptosis induction in both the mitochondrion and the ER.

关键词： mesenchymal stem cells serum deprivation mitochondria pathway endoplasmicreticulum stress synergistic effects

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Intestinal microbiota and juvenile idiopathic arthritis:current understanding and future prospective

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World Journal of Pediatrics 2021年第1期17卷 40-51页

作者： Le Xin Feng He Sen Li Zhi-Xuan Zhou xiao-lin ma Gynecological Minimal Invasive Center Beijing Obstetrics and Gynecology HospitalCapital Medical UniversityBeijingChina Department of Biochemistry and Immunology Capital Institute of PediatricsYabao Road No.2Chaoyang DistrictBeijingChina Department of Rheumatology Capital Institute of PediatricsBeijingChina

Background Juvenile idiopathic arthritis(JIA)characterized by arthritis of unknown origin is the most common childhood chronic rheumatic disease,caused by both host genetic factors and environmental *** evidence has mounted to focus on the intestinal microbiota,a potentially recognized set of environmental triggers affecting JIA *** we offer an overview of recently published animal and human studies that support the impact of intestinal microbiota in *** sources We searched PubMed for animal and human studies publications with the search terms"intestinal microbiota or gut microbiota"and"juvenile idiopathic arthritis or juvenile chronic arthritis or juvenile rheumatoid arthritis or childhood rheumatoid arthritis or pediatric rheumatoid arthritis".Results Several comparative studies have demonstrated that intestinal microbial alterations might be triggers in disease ***,a slice of studies has suggested environmental triggers in early life might disrupt intestinal microbial colonization,including cesarean section,formula feeding,and antibiotic *** intestinal microbiota may influence the development of JIA by mediating host immune programming and by altering mucosal *** Specific microbial factors may contribute to the pathogenesis of *** studies,however,are warranted to investigate the causality between intestinal dysbiosis and JIA and the mechanisms behind these epidemiologic *** are also needed to design the best interventional administrations to restore balanced intestinal microbial communities.

关键词： Antibiotic exposure Cesarean section Immune system reprogramming Intestinal microbiota Juvenile idiopathic arthritis

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THE STUDY OF THE YELLOW SEA WARM CURRENT AND ITS SEASONAL VARIABILITY

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Journal of Hydrodynamics 2009年第2期21卷 159-165页

作者： XU ling-ling WU De-xing lin xiao-pei ma Chao Physical Oceanography Laboratory Ocean University of China Qingdao 266100 China

The Yellow Sea Warm Current （YSWC） penetrates northward along the Yellow Sea Trough, and brings warm and saline water towards the Bohai Sea. The YSWC becomes much less intrusive in summer and is limited mostly in the southern trough, contrasting with a deep winter penetration well into the trough. The seasonal variability of the YSWC has prompted a debate regarding which controls the YSWC and its seasonal variability. In this article, the annual mean and seasonal variability of the YSWC was examined by using a 3-D ocean model together with several experiments. The results show that in the annual mean the YSWC is a compensating current firstly for the southward Korea Coastal Current （KCC）, which is mainly caused by the Kuroshio Current （KC）. The local wind-stress forcing plays an important but secondary role. However, the local monsoonal forcing plays a prominent role in modulating the seasonal variability. A deep northwestward intrusion of the YSWC in winter, for instance, is mainly due to a robustly developed China Coastal Current （CCC） which draws water along the Yellow Sea trough to feed a southward flow all the way from the Bohai Sea to the Taiwan Strait.

关键词： Yellow Sea Warm Current (YSWC) Kuroshio local wind monsoon

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Effect of electro-acupuncture on substance P, its receptor and corticotropin-releasing hormone in rats with irritable bowel syndrome

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World Journal of Gastroenterology 2009年第41期15卷 5211-5217页

作者： xiao-Peng ma lin-Ying Tan Yun Yang Huan-Gan Wu Bin Jiang Hui-Rong Liu ling Yang Shanghai Institute of Acupuncture-Moxibustion and Meridian Shanghai 200030 China

AIM： To investigate the effect and mechanism of electro-acupuncture lEA） at ST25 and ST37 on irritable bowel syndrome （IBS） of rats. METHODS： A total of 21 male Sprague-Dawley rats were randomly divided into normal group, model group and EA group. A rat model of IBS was established by constraining the limbs and distending the colorectum of rats. Rats in EA group received bilateral EA at ST25 and ST37 with a sparse and intense waveform at a frequency of 2/50 Hz for 15 min, once a day for 7 d as a course. Rats in normal and model groups were stimulated by distending colorectum （CR）. An abdominal withdrawal reflex （AWR） scoring system was used to evaluate improvements in visceral hypersensitivity. Toluidine blue-improved method, immunohistochemistry and radioimmunoassay were used to observe mucosal mast cells （MC）, changes of substance P （SP） and substance P receptor （SPR） in colon and change of corticotropin-releasing hormone （CRH） in hypothalamus. RESULTS： The threshold of visceral sense was significantly lower in model group than in normal group,and significantly higher in EA group than in model group. The number of mucosal MC was greater in model group than in normal group and significantly smaller in EA group than in model group. The CRH level in hypothalamus of rats was significantly higher in model group than in normal group, which was remarkably decreased after electro-acupuncture treatment. The SP and SPR expression in colon of rats in model group was decreased after electro-acupuncture treatment. CONCLUSION： EA at ST25 and ST37 can decrease the number of mucosal MC and down-regulate the expression of CRH in hypothalamus, and the expression of SP and SPR in colon of rats with IBS.

关键词： Electro-acupuncture Corticotropin-releasing hormone Irritable bowel syndrome Substance P Substance P receptor

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Rapamycin instead of mycophenolate mofetil or azathioprine in treatment of post-renal transplantation urothelial carcinoma

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Chinese Medical Journal 2009年第1期122卷 35-38页

作者： HU xiao-peng ma lin-lin WANG Yong YIN Hang WANG Wei YANG xiao-yong ZHANG xiao-dong Department of Urology Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China

Background malignant tumor is the most common complication occurred in transplant recipients. It is widely recognized that immunosuppressive treatments increase the risk of cancer in transplant recipients. The efficacy and safety of rapamycin （RPM） in combination with low-dose calcineurin inhibitor （CNI） in treating 15 renal allograft recipients which developed urothelial carcinoma were observed.Methods Immunosuppressive regimen in all recipients was altered with rapamycin to replace mycophenolate mofetil （MMF） or azathioprine （Aza）. The initial loading dosage was 2 mg/d, and the next dosage was 1 mg/d. The dosage of rapamycin was carefully adjusted according to the blood drug level and concentration of the drug was maintained at 4-6 μg/L. In all the 15 patients, the calcineurin inhibitor was reduced down to one third of the original dosage after the rapamycin blood concentration became stable. Surgical treatment and intravesical instillation chemotherapy were carried out in all patients. Recurrence of the tumor was monitored throughout the study. Post-transplant renal function and side effects were also closely monitored.Results Among the 15 patients, 9 had no tumor recurrence in 2 years, 2 had tumor recurrences twice, and 4 had once. There was no acute rejection observed during RPM treatment. Post-transplant renal function in 11 patients was improved with a decreased creatinine level. Hyperlipoidemia and thrombocytopenia were the most frequent adverse events which responded well to corresponding treatments.Conclusion Among the renal allograft recipients with urothelial carcinoma, combination of rapamycin and low dose calcineurin inhibitor treatment is effective and safe.

关键词： renal transplantation urothelial carcinoma rapamycin

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弹道明胶老化初级阶段的模型

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高分子学报 2017年第8期27卷 1382-1388页

作者：马小林范毓润浙江大学机械工程学院流体动力及机电系统国家重点实验室杭州310027

用流变震荡模式研究了10 wt%浓度的弹道明胶在冷却和等温下的老化行为.对明胶从sol-gel点冷却到某个目标温度的过程,通过不同冷却速率的实验,建了一个弹性模量与温度的线性关系.不同温度下明胶的等温老化实验表明,弹性模量-时间曲线具... 详细信息

用流变震荡模式研究了10 wt%浓度的弹道明胶在冷却和等温下的老化行为.对明胶从sol-gel点冷却到某个目标温度的过程,通过不同冷却速率的实验,建了一个弹性模量与温度的线性关系.不同温度下明胶的等温老化实验表明,弹性模量-时间曲线具有相似的形状.依据Normand提出的二级反应动力学模型,引入一个表征明胶老化行为的速率常数,构建了一个预测弹道明胶在老化初级阶段的弹性模量演化的模型.该模型中模量-温度关系在初始和无穷大时是直线,其他模量-温度线是近似直线,并交于sol-gel点.老化速率常数和过冷度之间符合Flory-Weaver方程.对弹性模量和老化时间进行归一化处理,可将不同温度下的老化曲线叠加成一条主曲线.

关键词：弹道明胶冷却模型等温老化模型二级反应动力学老化速率常数

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Clinical characteristics of fulminant hepatitis in pregnancy

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World Journal of Gastroenterology 2005年第29期11卷 4600-4603页

作者： xiao-mao Li lin ma Yue-Bo Yang Zhong-Jie Shi Shui-Sheng Zhou Department of Obstetrics and Gynecology Third Affiliated Hospital Sun Yat-Sen University Guangzhou 510630 Guangdong Province China

AIM： To investigate the clinical characteristics of fulminant hepatitis in pregnancy. METHODS： We compared and analyzed the etiology, clinical characteristics, and laboratory examinations of 25 cases of fulminant hepatitis in pregnancy and 30 cases of fulminant hepatitis not in pregnancy. RESULTS： HBV infection and chronic fulminant hepatitis were most common both in the pregnant and in the non-pregnant groups. Jaundice, digestive tract symptoms, increase of bilirubin and thrombinogen activity were the main manifestations. The incidence of hepatic encephalopathy （HE） and hepato-renal syndrome （HRS） was significantly different between the two groups. The incidence of preterm labor, dead fetus and neonatal asphyxia was high. CONCLUSION： Fulminant hepatitis is likely to occur in late pregnancy wibh more severe complications, which significantly influences maternity, perinatal fetus, and newborn.

关键词： Pregnancy Fulminant hepatitis Clinical characteristics

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Prognostic factors of fulminant hepatitis in pregnancy

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Chinese Medical Journal 2005年第20期118卷 1754-1757页

作者： LI xiao-mao ma lin YANG Yue-bo SHI Zhong-jie ZHOU Shui-sheng Department of Obstetrics and Gynecology Third Affiliated HospitalSun Yat-Sen University Guangzhou 510630 China

Fulminant hepatitis （FH） refers to liver diseases that have severe state and complicated manifestations. It will endanger patients＇lives and health largely. The incidence of fulminant hepatitis in pregnancy （FHP） is 66 times of patients not in pregnancy,1 which is more dangerous and is one of the major causes of maternal and perinatal death in China. In order to predict the prognoses of FHP and decrease maternal and perinatal death rate, we summarized clinical information of 25 cases of FHP admitted to our hospital and investigated into prognostic factors that influenced FHP.

关键词： fulminant hepatitis · pregnancy · prognosis

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Effects of CO2 pneumoperitoneum on blood flow vol-ume of abdominal organs of rabbits with controlled hem-orrhagic shock and liver impact injuries

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Chinese Journal of Traumatology 2009年第1期12卷 -页

作者： Lian-yang ZHANG[a] Song ZHAO[a] Yong LI[a] xiao-lin ma[a] Department of Traumatic Surgery Research Institute of Surgery/Daping Hospital Third Military Medical UniversityChongqing 400042 China

Objective: To investigate the effects of CO2 pneumo-peritoneum on blood flow volume of abdominal organs of rabbits with controlled hemorrhagic shock model and liver impact ***: After controlled hemorrhagic shock and liver impact injuries, the rabbit model was established. Eighteen rabbits subjected to hemorrhagic shock and liver impact inju-ries were divided into 3 groups randomly according to the volume of lost blood: light hemorrhagic shock (blood loss volume was 10%, 6 ml/kg), moderate hemorrhagic shock (20%, 12 ml/kg) and severe hemorrhagic shock (40%, 22 ml/kg). Intraabdominal pressures of CO2 pneumoperitoneum was 10 mmHg. Color-labeled microspheres were used to mea-sure the blood flow volume of the liver, kidney and stomach before pneumoperitoneum at 30 minutes and 2 hours after pneumoperitoneum and 30 minutes after deflation. And the mortality and hepatic traumatic condition of rabbits were ***: Of the 18 rabbits, there were 9 with liver impact injuries at Grade Ⅰ, 8 at Grade Ⅱ and Ⅰ at Grade Ⅲ (according to AIS-2005). The mortality rate in light hemorrhagic shock group was 33.33%, and that in moderate or severe hemor-rhagic shock group was 100% within 30 minutes and 2 hours after pneumoperitoneum, respectively. The blood flow vol-ume in the organs detected decreased at 30 minutes under pneumoperitoneum in light and moderate hemorrhagic shock groups. At the same time, the blood flow volume of the liver in moderate hemorrhagic shock group decreased more sig-nificantly than that in light hemorrhagic shock ***: The blood flow volume of abdominal organs in rabbits is decreased obviously under CO2 pneumoperitoneum, with fairly high mortality rate. It is be-lieved that CO2 pneumoperitoneum should cautiously be used in abdominal injury accompanied with hemorrhagic shock, especially under non-resuscitation conditions.

关键词： Abdominal injuries Shock,hemorrhagic Pneumoperitoneum,artificial Regional blood flow

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颗粒间的毛细作用以及吸应力特征曲线分析

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岩土力学 2017年第7期38卷 2036-2042页

作者：周凤玺曹小林马强兰州理工大学土木工程学院甘肃兰州730050 兰州理工大学西部土木工程防灾减灾教育部工程研究中心甘肃兰州730050

颗粒之间的毛细作用是分析湿颗粒体系力学特性的一个重要方面。考虑两球形颗粒与粒间弯液面的相互作用,采用打靶法求解得到了粒间气-液-固交界面的几何形状与基质吸力关系的精确解答,并与环形近似方法和数据拟合方法得到的粒间作用力进... 详细信息

颗粒之间的毛细作用是分析湿颗粒体系力学特性的一个重要方面。考虑两球形颗粒与粒间弯液面的相互作用,采用打靶法求解得到了粒间气-液-固交界面的几何形状与基质吸力关系的精确解答,并与环形近似方法和数据拟合方法得到的粒间作用力进行了比较,分析了两种简化方法的精确度。在此基础上,分析了不同粒间间距、液-固接触角以及基质吸力时,两球形颗粒间由毛细作用引起的吸应力特征曲线。分析结果表明,当液体体积较小时,拟合方法得到的结果更好,而当液体体积较大时,采用环形近似得到的结果要比拟合方法更接近精确解,为非饱和土中吸力的认识以及非饱和土力学微观分析提供了一定的理论指导。

关键词： Young—Laplace方程非饱和土液桥吸应力特征曲线有效应力原理

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