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Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism

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Asian Journal of Andrology 2010年第5期12卷 744-752页

作者： Jian Zhang xiao-jun wu De-Xiang Zhuo Tao Liu Wei-Ren Li Ze-Bin Mao Zhong-Cheng Xin Andrology Center First Hospital Peking University Beijing 100009 China Institute of Urinary Surgery Southwest Hospital Third Military Medical University Chongqing 400038 China The Department of Biochemistry and Molecular Biology Health Science Center Peking University Beijing 100191 China

This study compared tankyrase 1 expression and autophagy quantity between erectile dysfunction （ED） and non-ED rats＇ corpus cavernosum smooth muscle cells （CSMCs）. This study aslo explored the effect and possible mechanism of tankyrase 1 on autophagy and cell proliferation in ageing ED rats＇ CSMCs. The intracavernous pres- sure and mean systemic arterial pressure were measured to investigate erectile function so that eight 24-month-old ED and eight 8-month-old male Wistar rats were choosed respectively. The rat CSMCs were isolated and cultured by enzyme digestion, in which tankyrase 1 expression and autophagy quantity were compared. Tankyrase 1 over-expression was induced with plasmid transfection by Lipofectamine^TM. The effect of tankyrase 1 overexpression on proliferation, autophagy and mTOR pathway in 24-month-old ED rats＇ CSMCs was measured by the cell growth curve in MTT assay, cell cycle analysis in flow cytometry （FCM）, key protein expression in Western blot, autophagy quantity in transmission electron microscopy, monodansylcadaverine staining and GFP-LC3 fluorescence. The primary CSMCs were confirmed by immunofluorescence, and the purity was 99.1% in FCM. Compared with that of 8-month-old rats, tankyrase 1 expression and autophagy quantity significantly decreased in 24-month-old ED rats＇ primary CSMCs （P 〈 0.01）. Tankyrase 1 overexpression significantly increased the growth rate （P 〈 0.05） and increased the S phase of cell cycle （P 〈 0.01）. The autophagosome quantity was remarkably increased （P 〈 0.01）, LC3-Ⅰ/Ⅱ and Beclin 1 were upregulated （P 〈 0.01 and P 〈 0.05）, and p-p70S6K （Thr389） was downregulated in 24-month-old ED rat CSMCs （P 〈 0.05）. In conclusion, Tankyrase 1 and autophagy decrease in the CSMCs from aging rats with ED, and tankyrase 1 may have a positive effect on proliferation by enhancing autophagy and regulating the mTOR signalling pathway.

关键词： ageing autophagy corpora cavernosum corpus cavemosum smooth muscle cells erectile dysfunction senile tankyrase

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First-Principles Study of Ultrathin Molybdenum Sulfides Nanowires:Electronic and Catalytic Hydrogen Evolution Properties

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Chinese Journal of Chemical Physics 2019年第2期32卷 267-272,I0003页

作者： xiao-ting Li Yang-yang Wan xiao-jun wu Hefei National Laboratory of Physical Sciences at the Microscale School of Chemistry and Materi-als Sciences and CAS Key Laboratory of Materials for Energy Conversion University of Science and Technology of China Hefei 230026 China

Molybdenum sulfides nanomaterials, such as one-dimensional (1D) nanotubes, nanoribbons, and two-dimensional (2D) nanosheets, have attracted intensive research interests for their novel electronic, optical, and catalytic properties. On the basis of first-principles calculation, here, we report a new series of 1D ultrathin molybdenum sulfides nanowires, including Mo2S6、Mo3S6 and Mo6S10 nanowires. Our results demonstrate that these ultrathin nanowires are both thermal and lattices dynamically stable, confirmed with the calculated phonon spectrum and Born-Oppenheimer molecular dynamic simulation at the temperature up to 600 K. The calculated elastic constant is 21.33, 103.22, and 163.00 eV/■ for Mo2S6, Mo3S6, and Mo6S10 nanowires, respectively. Mo2S6 and Mo3S6 nanowires are semiconductors with band gap of 1.55 and 0.46 eV, while Mo6S10 nanowires is metal, implying their potential applications in electronics and optoelectronics. In particular, ultrathin molybdenum sulfides nanowires can be used as catalysts for hydrogen evolution reaction. The calculated Gibbs free energy change for hydrogen evolution is about -0.05 eV for Mo2S6 nanowire, comparable with those of Pt and H-MoS2. The prediction of these 1D molybdenum sulfides nanowires may enrich the 1D family molybdenum sulfides and make a supplement to understand the high performance of hydrogen evolution reaction in transition-metal dichalcogenides.

关键词： First-principles calculations Molybdenum sulfide nanowires Hydrogen evolution reaction

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Clinical outcome of treating intractable hematospermia using holmium laser incision through a ureteroscope

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Asian Journal of Andrology 2016年第1期18卷 140-142页

作者： xiao-jun wu Heng Zhang Yong-Quan Wang Yi Zhi Ji Zheng Peng He Wei-Bing Li Zhan-Song Zhou Institute of Urinary Surgery Southwest Hospital Third Military Medical University Chongqing 400038 China

Aconventional procedure for treating hematospermia is to insert a ureteroscope or seminal vesiculoscope into the prostatic utr/de through the urethra, and then dilate the ejaculatory duct opening using the scope or the catheter. However, the ejaculatory duct stenosis may occur after the simple ureteroscopy. We compared the outcomes of treating hematospermia using holmium laser incision through a ureteroscope and using simple ureteroscopy. Retrospective method was used to analyze 67 intractable hematospermia cases. Varying degrees of ejaculatory duct stenosis or obstruction were observed in all the patients. Postoperative follow-up was conducted for 6 months up to 3 years. Two patients who underwent the simple ureteroscopy experienced hematospermia recurrence in 6 and 8 months, respectively. No recurrence was found in all the patients who underwent the holmium laser incision. We have found that the holmium laser incision through a ureteroscope shows a better outcome than the simple ureteroscop~

关键词： @@

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Mast Cells in Adjacent Normal Colon Mucosa rather than Those in Invasive Margin are Related to Progression of Colon Cancer

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Chinese Journal of Cancer Research 2011年第4期23卷 276-282页

作者： Qing Xia Ya Ding xiao-jun wu Rui-qing Peng Qiang Zhou Jing Zeng Jing-hui Hou Xing Zhang Yi-xin Zeng xiao-shi Zhang Ying-bo Chen State Key Laboratory of Oncology in South China Cancer Center Sun Yat-sen University Guangzhou 510060 China Department of Medical Oncology Cancer Center Sun Yat-sen University Guangzhou 510060 China Biotherapy Center Cancer Center Sun Yat-sen University Guangzhou 510060 China Department of Colorectal Oncology Cancer Center Sun Yat-sen University Guangzhou 510060 China Department of Medical Oncology the First People Hospital of Yueyang Yueyang 414000 China Department of Pathology Cancer Center Sun Yat-sen University Guangzhou 510060 China Department of Gastric and Pancreatic Oncology Cancer Center Sun Yat-sen University Guangzhou 510060 China

Objective: Mast cells (MC) reside in the mucosa of the digestive tract as the first line against bacteria and toxins. Clinical evidence has implied that the infiltration of mast cells in colorectal cancers is related to malignant phenotypes and a poor prognosis. This study compared the role of mast cells in adjacent normal colon mucosa and in the invasive margin during the progression of colon cancer. Methods: Specimens were obtained from 39 patients with colon adenomas and 155 patients with colon cancers treated at the Sun Yat-sen University Cancer Center between January 1999 and July 2004. The density of mast cells was scored by an immunohistochemical assay. The pattern of mast cell distribution and its relationship with clinicopathologic parameters and 5-year survival were analyzed. Results: The majority of mast cells were located in the adjacent normal colon mucosa, followed by the invasive margin and least in the cancer stroma. Mast cell count in adjacent normal colon mucosa (MCCadjacent) was associated with pathologic classification, distant metastases and hepatic metastases, although it was not a prognostic factor. In contrast, mast cell count in the invasive margin (MCCinvasive) was associated with neither the clinicopathlogic parameters nor overall survival. Conclusion: Mast cells in the adjacent normal colon mucosa were related to the progression of colon cancer, suggesting that mast cells might modulate tumor progression via a long-distance mechanism.

关键词： Mast cell Colon cancer Mucosa Invasive margin Prognosis

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Reprogramming immunosuppressive myeloid cells by activated T cells promotes the response to anti-PD-1 therapy in colorectal cancer

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Signal Transduction and Targeted Therapy 2021年第2期6卷 447-460页

作者： Jing Chen Hong-Wei Sun Yan-Yan Yang Hai-Tian Chen Xing Juan Yu Wen Chao wu Yi-Tuo Xu LiLian Jin xiao-jun wu Jing Xu Limin Zheng State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhouChina MOE Key Laboratory of Gene Function and Regulation School of Life SciencesSun Yat-sen UniversityGuangzhouChina First Afliated Hospital Sun Yat-sen UniversityGuangzhouChina Department of Medical Oncology Dana Farber Cancer InstituteBostonMA.USA

Overcoming local immunosuppression is critical for immunotherapy to produce robust anti-tumor ***-derived suppressor cells(MDSCS)are key regulators of immunosuppressive networks and promote tumor ***,it remains unclear whether and how tumor-infltrating MDSCS are shaped in response to anti-PD-1 treatment and what their impact on therapeutic efficacy is in colorectal cancer(CRC).In this study,the levels of infltrating MDSCS were significantly higher in the non-responding organoids and were selectively reduced in the responding group,with MDSCS showing increased apoptosis and attenuated functional activity after anti-PD-1 treatment.A negative correlation between T-cell activation and MDSC function was also observed in fresh human CRC *** studies revealed that autocrine IFN-α/B upregulated TRAIL expression on activated T cells to elicit MDSC apoptosis via the TRAIL-DR5 interaction and acted synergistically with TNF-α to inhibit MDSC function of suppressing the T-cell response through the JNK NMDAR-ARG-1 ***,blockade of IFNa/βand TNF-α abolished the therapeutic efficacy of anti-PD-1 treatment by preserving the frequency and suppressive activity of infltrating MDSCS in a CRC mouse *** result suggested that reprogramming MDSCS by IFN-α/βand TNF-α from activated T cells was necessary for succesful anti-PD-1 treatment and might serve as a novel strategy to improve the response and efficacy of anticancer therapy.

关键词： colorectal cancer programming

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Adaptive Modulation with Channel Estimation in High-Speed Packet-Based OFDM Communication Systems

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The Journal of China Universities of Posts and Telecommunications 2004年第z1期11卷 76-82页

作者： HOU xiao-lin wu jun-li YIN Chang-chuan YUE Guang-xin School of Telecommunication Engineering Beijing University of Posts and Telecommunications Beijing 100876 P.R.China School of Telecommunication Engineering Beijing University of Posts and Telecommunications Beijing 100876 P.R.China School of Telecommunication Engineering Beijing University of Posts and Telecommunications Beijing 100876 P.R.China School of Telecommunication Engineering Beijing University of Posts and Telecommunications Beijing 100876 P.R.China

In this paper, adaptive modulation with channel estimation in high-speed packet-based Orthogonal Frequency Division Multiplexing (OFDM) communication systems for beyond 3G are discussed. Different adaptive modulation and channel estimation methods are presented and compared, then those methods suitable for our intended application are chosen. Conclusions can be drawn from computer simulations that with proper selection of packet length and subband width, wide subband adaptive modulation with Least Square plus Discrete-time Fourier Transform (LS-DFT) channel estimation can give an acceptable performance with low complexity for channel with low Doppler shift and small path delay. Otherwise, narrow subband or subcarrier adaptive modulation together with LS-DFT plus Decision Directed (LS-DFT-DD) must be used.

关键词： OFDM adaptive modulation channel estimation beyond 3G

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High-Performance Chemical Information Database towards Accelerating Discovery of Metal-Organic Frameworks for Gas Adsorption with Machine Learning

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Chinese Journal of Chemical Physics 2021年第4期34卷 436-442,I0003页

作者： Zi-kai Hao Hai-feng Lv Da-yong Wang xiao-jun wu Hefei National Laboratory for Physical Science at the Microscale School of Chemistry and Materials SciencesCAS Key Laboratory of Materials for Energy Conversionand CAS Center for Excellence in NanoscienceUniversity of Science and Technology of ChinaHefei 230026China Synergetic Innovation Center of Quantum Information&Quantum Physics University of Science and Technology of ChinaHefei 230026China

Chemical structure searching based on databases and machine learning has at-tracted great attention recently for fast screening materials with target *** this end,we estab-lished a high-performance chemical struc-ture database based on MYSQL engines,named *** than 160000 metal-organic frameworks(MOFs)have been collected and stored by using new retrieval algorithms for effcient searching and *** evaluations results show that MYDB could realize fast and effcient key-word searching against millions of records and provide real-time recommendations for similar *** machine learning method and materials database,we developed an adsorption model to determine the adsorption capacitor of metal-organic frameworks to-ward argon and hydrogen under certain *** expect that MYDB together with the developed machine learning techniques could support large-scale,low-cost,and highly convenient structural research towards accelerating discovery of materials with target func-tionalities in the eld of computational materials research.

关键词： Chemical informatics Database Search engine Machine learning Gas ab-sorption

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Oxaliplatin-containing adjuvant chemotherapy improves the survival of locally advanced rectal cancer patients with pathological complete response after pre-operative chemoradiotherapy

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Gastroenterology Report 2018年第3期6卷 195-201,I0002页

作者： Jian-Hong Peng jun-Zhong Lin Yu-Ming Rong Ying Zhu Yu-Xiang Deng Yu-Jie Zhao Zhen-Hai Lu xiao-jun wu Zhi-Zhong Pan Department of Colorectal Surgery Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer Medicine651 Dongfeng Road EastGuangzhouGuangdong 510060P.R.China VIP Region Sun Yat-sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer Medicine651 Dongfeng Road EastGuangzhouGuangdong 510060P.R.China

Background:The necessity for adjuvant chemotherapy(ACT)in locally advanced rectal cancer(LARC)patients who achieve pathological complete response(pCR)after pre-operative chemoradiotherapy(CRT)is still not *** aimed to investigate the therapeutic value of ACT in these ***:Clinical data were retrospectively collected from 105 consecutive LARC patients who achieved pCR after pre-operative CRT and underwent radical tumor resection between December 2008 and April 2014 in a comprehensive cancer *** chemotherapy(CT)was administered by combining oxaliplatin with capecitabine(XELOX regimen).Disease-free survival(DFS)and overall survival(OS)rates of patients with or without ACT were ***:Eighty-three(79.0%)patients received ACT and 22(21.0%)did *** a median follow-up of 49 months,the ACT group had a significantly higher 3-year DFS rate(92.8 vs 86.4%,p=0.029)and 3-year OS rate(95.1 vs 86.1%,p=0.026)than the non-ACT *** multivariable analyses,the presence of ACT was an independent prognostic factor for DFS(hazard ratio[HR]:0.271;95%confidence interval(CI):0.080–0.916;p=0.036)but not for *** benefit was more obvious in patients younger than 60 years via subgroup analysis(adjusted HR:0.106;95%CI:0.019–0.606;p=0.012).Conclusions:Oxaliplatin-containing ACT may confer survival benefits to patients with pCR,particularly younger ***,the routine use of ACT in patients with pCR needs further validation.

关键词： Adjuvant chemotherapy rectal cancer pre-operative chemoradiotherapy pathological complete response survival

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Molecular Epidemiology and Risk Factors of Carbapenem-Resistant Klebsiella Pneumoniae Bloodstream Infections in wuhan,China

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Current Medical Science 2022年第1期42卷 68-76页

作者： Chan LIU Lan LIU Ming-ming JIN Yang-bo HU Xuan CAI Lu WAN Hai-yue ZHANG Rui-yun LI xiao-jun wu Department of Respiratory and Critical Care Medicine Renmin Hospital of Wuhan UniversityWuhan 430060China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430060China Department of Clinical Laboratory Renmin Hospital of Wuhan UniversityWuhan 430060China

Objective:The clinical characteristics and microbiological data of patients with *** bloodstream infections(BSI)from January 2018 to December 2020 were retrospectively analyzed to study the molecular epidemiology of Carbapenem-resistant Klebsiella pneumoniae(CRKP).We also aimed to identify the risk factors for the development of CRKP ***:This retrospective study was conducted at Renmin Hospital of wuhan University from January 2018 to December *** date of non-duplicate *** isolates isolated from blood samples was identified using the microbiology laboratory *** data from patients diagnosed with *** BSI were collected and analyzed.

关键词： Carbapenem-resistant Klebsiella pneumoniae bloodstream infection

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Geomagnetic diurnal-variation anomalies and their relation to strong earthquakes

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Acta Seismologica Sinica(English Edition) 2004年第z1期17卷 85-93页

作者： DING Jian-hai(丁鉴海) LIU Jie(刘杰) YU Su-rong(余素荣) xiao wu-jun(肖武军) Institute of Earthquake Science China Earthquake Administration Beijing 100036 China

The diurnal-variation anomalies of the vertical-component in geomagnetic field are mainly the changes of phase and amplitude before strong earthquakes. On the basis of data recorded by the network of geomagnetic observato- ries in China for many years, the anomalous features of appearance time of the minima of diurnal variations (i.e, low-point time) of the geomagnetic vertical components and the variation of their spatial distribution (i.e, phe- nomena of low-point displacement) have been studied before over 30 strong earthquakes with MS≥6.6 such as Kunlunshan MS=8.1 earthquake on November 14, 2001; Bachu-Jashi MS=6.8 earthquake on February 24, 2003; xiaojin MS=6.6 earthquake on September 22, 1989, etc. There are good relations between such rare phenomena of geomagnetic anomalies and the occurrence of earthquakes. It has been found that most earthquakes occur in the vicinity of the boundary line of sudden change of the low-point displacement and generally within four days before and after the 27th or 41st day counting from the day of appearance of the anomaly. In addition, the anomalies of diurnal-variation amplitude near the epicentral area have been also studied before Kunlunshan MS=8.1 earthquake and Bachu-Jiashi MS=6.8 earthquake.

关键词： geomagnetic diurnal-variation anomalies geomagnetic low-point displacement diurnal-variation amplitude strong earthquake

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