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陆生野生动物疫源疫病监测对象确定方法研究

林业调查规划 2017年第4期42卷 55-60,63页

作者：肖军杨启鸿金志堃文明云南森林自然中心云南昆明650224

围绕动物疫病传播的三要素:传染源、传播途径、易感动物,遵循陆生野生动物疫源疫病监测技术规范,进行陆生野生动物疫源特点特征的分析,查找出符合疫病传播三要素特点特征的动物疫源,结合疫病资料收集和实地调查,设置条件制作工具,实现... 详细信息

围绕动物疫病传播的三要素:传染源、传播途径、易感动物,遵循陆生野生动物疫源疫病监测技术规范,进行陆生野生动物疫源特点特征的分析,查找出符合疫病传播三要素特点特征的动物疫源,结合疫病资料收集和实地调查,设置条件制作工具,实现监测对象的确定,从而解决监测工作中监测对象不明确,确定方法不科学等相关问题。

关键词：陆生野生动物疫源疫病监测对象

Effects of ultrasonic-assisted extraction on bioactive compounds,volatile flavors and antioxidant activities of vine tea water extracts

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Traditional Medicine Research 2025年第1期10卷 73-81页

作者： xiao-Long Zhou Wei-Jin Jiang Ji Yu Mao-jun Yao Yun-Tong Li Hunan Provincial Key Laboratory of Research Resource Mining and High-valued Utilization on Edible&Medicinal PlantCollege of Chemistry and Chemical EngineeringJishou UniversityJishou 416000China Hunan Gaoge Dairy Co. Ltd.Changsha 410000China

Background:Ampelopsis grossedentata,vine tea,which is the tea alternative beverages in *** vine tea processing,a large amount of broken tea is produced,which has low commercial ***:This study investigates the influence of different extraction methods(room temperature water extraction,boiling water extraction,ultrasonic-assisted room temperature water extraction,and ultrasonic-assisted boiling water extraction,referred to as room temperature water extraction(RE),boiling water extraction(BE),ultrasonic assistance at room temperature water extraction(URE),and ultrasonic assistance in boiling water extraction(UBE))on the yield,dihydromyricetin(DMY)content,free amino acid composition,volatile aroma components,and antioxidant properties of vine tea ***:A notable influence of extraction temperature on the yield of vine tea extracts(P<0.05),with BE yielding the highest at 43.13±0.26%,higher than that of RE(34.29±0.81%).Ultrasound-assisted extraction significantly increased the DMY content of the extracts(P<0.05),whereas DMY content in the RE extracts was 59.94±1.70%,that of URE reached 66.14±2.78%.Analysis revealed 17 amino acids,with L-serine and aspartic acid being the most abundant in the extracts,nevertheless ultrasound-assisted extraction reduced total free amino acid *** chromatography-mass spectrometry analysis demonstrated an increase in the diversity and quantity of compounds in the vine tea water extracts obtained through ultrasonic-assisted ***,69 and 68 volatile compounds were found in URE and UBE extracts,which were higher than the number found in RE and BE *** vitro,antioxidant activity assessments revealed varying antioxidant capacities among different extraction methods,with RE exhibiting the highest DPPH scavenging rate,URE leading in ABTS•+free radical scavenging,and BE demonstrating superior ferric ion reducing antioxidant ***:The findings suggest that extraction methods signi

关键词： vine tea dihydromyricetin ultrasonic-assisted extraction volatile aroma components

甘肃省武威市农村妇女宫颈癌与乳腺癌筛查结果分析

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疾病预防控制通报 2016年第5期31卷 8-11页

作者：杨天琳李晓春杨晓霞严小妍杨峻山甘肃省武威市疾病预防控制中心甘肃武威733000 武威市妇幼保健计划生育服务中心甘肃武威733000 武威市人民医院甘肃武威733000

目的了解2009─2015年甘肃省武威市农村妇女宫颈癌和乳腺癌筛查情况,探讨武威市＂两癌＂流行趋势与特点,为推广筛查工作提供科学依据。方法采取自愿原则,在武威市35~64岁已婚农村妇女中筛查＂两癌＂;通过妇科常规、宫颈脱落细胞液基薄... 详细信息

目的了解2009─2015年甘肃省武威市农村妇女宫颈癌和乳腺癌筛查情况,探讨武威市＂两癌＂流行趋势与特点,为推广筛查工作提供科学依据。方法采取自愿原则,在武威市35~64岁已婚农村妇女中筛查＂两癌＂;通过妇科常规、宫颈脱落细胞液基薄层细胞、阴道镜和组织病理学等检查方法筛查宫颈癌,以常规视诊、触诊、彩超、钼靶—X线和病理检查方法筛查乳腺癌。结果 2009─2015年武威市农村妇女妇科疾病检查250 110人,患病134 224人,患病率53.67%;患病率2012年最高（61.80%）,2014年最低（37.00%）,呈逐年下降趋势,差异有统计学意义（Z=-29.718 7,P〈0.000 1）;宫颈癌检出率为4.00%,早诊率90.30%,癌前病变（CINⅠ、Ⅱ、Ⅲ级）检出率16.89%（999/5 918）;乳腺癌检出率3.08/10万。结论武威市农村妇女＂两癌＂患病呈高发态势,但癌前病变的早期检出降低了＂两癌＂的致死率,提高广大农村妇女的生存和生活质量,探索建立武威市＂两癌＂的防治模式和协作机制已势在必行。

关键词：宫颈癌乳腺癌患病率筛查

舰载导弹武器系统对基准信息的应用需求与展望

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战术导弹控制技术 2009年第2期16卷 45-49页

作者：杨小军杨继红徐兴柱海军特装转运办事处秦皇岛066004 海军驻航天三院军事代表室北京100074

在海军武器装备中，惯导系统的应用越来越广泛。武器系统应用惯导系统将不能回避在动基座条件下进行初始对准这一难题，而动基座对准必须利用外界的基准信息。本文对基准信息的现状和在导弹武器系统中的使用情况进行了介绍和分析，对未... 详细信息

在海军武器装备中，惯导系统的应用越来越广泛。武器系统应用惯导系统将不能回避在动基座条件下进行初始对准这一难题，而动基座对准必须利用外界的基准信息。本文对基准信息的现状和在导弹武器系统中的使用情况进行了介绍和分析，对未来武器系统中的惯导系统完成初始对准时对基准信息的需求和使用方式进行了预测和展望。

关键词：惯导系统初始对准基准信息

“互联网+”背景下的高校微信公众平台发展现状及对策建议

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泰州职业技术学院学报 2016年第4期16卷 11-13页

作者：李晓娜杨俊杰泰州职业技术学院泰州海事局江苏泰州225300

"互联网+"是互联网技术与其他行业的跨界融合,微信公众平台是其实现融合的重要载体。文章讨论了高校微信公众平台的发展现状、积极作用和存在不足,提出了"互联网+"背景下高校微信公众平台的发展对策。

关键词：微信公众平台高校微信公众平台管理 “互联网+”

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提高鸭儿峡油田表外储量水驱效率的对策

新疆石油地质 2004年第3期25卷 308-309页

作者：李晓军杨勇赵金辉中国石油玉门油田分公司甘肃玉门735200

通过对玉门鸭儿峡油田表外储量、砂体几何形态、沉积韵律、连通特性等特征的研究,就不同沉积环境下所形成砂体的注水开发特征进行了详细论述,并提出了相应的油层压裂、酸化等开发工艺措施,认为,注水开发效果取决于砂体的非均质性,残余... 详细信息

通过对玉门鸭儿峡油田表外储量、砂体几何形态、沉积韵律、连通特性等特征的研究,就不同沉积环境下所形成砂体的注水开发特征进行了详细论述,并提出了相应的油层压裂、酸化等开发工艺措施,认为,注水开发效果取决于砂体的非均质性,残余油主要分布在低孔、低渗带内。因此,在调剖时应控制主要砂体的产能,同时要兼顾低产能砂体;选择压裂层位时,应压开低渗透层,以利于注入水的均匀推进。这一研究成果为改进玉门鸭儿峡油田开发方案及提高采收率提供了依据。

关键词：注水开发压裂堵水非均质性沉积韵律驱油效率

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing jun Wu Minmin xiao Fangqing Zhao Jing-Dong J.Han Qi Liu xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

Deciphering fungal metabolon coupling tandem inverse-electrondemand Diels-Alder reaction and semipinacol rearrangement for the biosynthesis of spiro polycyclic alkaloids

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Science China Chemistry 2025年第01期 288-296页

作者： Shuai Liu Wen-Qiang Xu Ying-Tong Di Man-Cheng Tang Ding-Kang Chen Ming-Ming Cao Yao-Wen Chang Hong-Yu Tang Chun-Mao Yuan jun-Bo yang Zhi-Li Zuo Han Guo Zi-Fei Xu Ying Zeng Yun-Dong Wu xiao-Jiang Hao Key Laboratory of Phytochemistry and Natural Medicines (Chinese Academy of Sciences) Kunming Institute of Botany Chinese Academy of Sciences University of Chinese Academy of Sciences Lab of Computational Chemistry and Drug Design State Key Laboratory of Chemical Oncogenomics Peking University Shenzhen Graduate School Shenzhen Bay Laboratory State Key Laboratory of Microbial Metabolism School of Life Sciences & Biotechnology Shanghai Jiao Tong University State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Molecular Biology Experimental Center Germplasm Bank of Wild Species in Southwest China Kunming Institute of Botany Chinese Academy of Sciences Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Research Unit of Chemical Biology of Natural Anti-Virus Products Chinese Academy of Medical Sciences

In the intricate process of natural product biosynthesis, a metabolon can enhance metabolic flux by associating sequential enzymes. A fungal metabolon, comprising of flavin-dependent monooxygenase Spe F and P450 monooxygenase Spe G, is identified in the biosynthesis of spiro polycyclic alkaloids(+)-notoamide B and its diastereomer(+)-versicolamide B. Using notoamide E as a substance, Spe F/Spe G metabolon can control the stereoselectivity of its 2,3-epoxidation, followed by hydrogen atom ion at C-17 to generate reactive epoxide tau-m A with dienyl iminium unit. Subsequently,(+)-notoamide B and(+)-versicolamide B are produced via tandem nonenzymatic inverse-electron-demand Diels-Alder reaction and semipinacol rearrangement. This provides the first example of metabolon in the biosynthesis of spiro-prenylated indole alkaloids.

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KAJ780燃气涡轮航空润滑油的研制

润滑油 2017年第5期32卷 18-22页

作者：李静徐小红杨俊杰中国石油兰州润滑油研究开发中心甘肃兰州730060 中国石油润滑油公司北京100028

文章对燃气涡轮航空发动机及其润滑油标准进行了简述。根据国外实验室提供的分析检测数据,以多元醇酯为基础油并添加抗氧剂、极压抗磨剂等功能添加剂研制的KAJ780燃气涡轮航空润滑油表现出优异的抗氧化腐蚀性、热安定性、高温氧化安定... 详细信息

文章对燃气涡轮航空发动机及其润滑油标准进行了简述。根据国外实验室提供的分析检测数据,以多元醇酯为基础油并添加抗氧剂、极压抗磨剂等功能添加剂研制的KAJ780燃气涡轮航空润滑油表现出优异的抗氧化腐蚀性、热安定性、高温氧化安定性、极压抗磨性、润滑油相容性和橡胶相容性,产品性能满足SAE AS5780A规格中标准型(SPC)产品主要性能指标。

关键词：航空燃气涡轮润滑油 SAE AS5780A