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Mir-30d increases intracellular survival of Helicobacter pylori through inhibition of autophagy pathway

World Journal of Gastroenterology 2016年第15期22卷 3978-3991页

作者： xiao-jun yang Ruo-Huang Si Yu-He Liang Bing-Qiang Ma Ze-Bin Jiang Bin Wang Peng Gao Department of General Surgery Gansu Provincial Hospital Department of General Surgery The People’s Hospital of Baoji City

AIM: To determine if mir-30 d inhibits the autophagy response to Helicobacter pylori(H. pylori) invasion and increases H. pylori intracellular ***: The expression of mir-30 d was detected by quantitative polymerase chain reaction(PCR), and autophagy level was examined by transmission electron microscopy, western blot, and GFP-LC3 puncta assay in human AGS cells and GES-1 cells. Luciferase reporter assay was applied to confirm the specificity of mir-30 d regulation on the expression of several core molecules involved in autophagy pathway. The expression of multiple core proteins were analyzed at both the m RNA and protein level, and the intracellular survival of H. pylori after different treatments was detected by gentamicin protection ***: Autophagy level was increased in AGS and GES-1 cells in response to H. pylori infection, which was accompanied by upregulation of mir-30 d expression(P < 0.05, vs no H. pylori infection). In the two gastric epithelial cell lines, mimic mir-30 d was found to repress the autophagy process, whereas mir-30 d inhibitor increased autophagy responseto H. pylori invasion. mir-30 d mimic decreased the luciferase activity of wild type reporter plasmids carrying the 3′ untranslated region(UTR) of all five tested genes(ATG2B, ATG5, ATG12, BECN1, and BNIP3L), whereas it had no effect on the mutant reporter plasmids. These five genes are core genes of autophagy pathway, and their expression was reduced significantly after mir-30 d mimic transfection(P < 0.05, vs control cells without mir-30 d mimic treatment). Mir-30 d mimic transfection and direct inhibition of autophagy increased the intracellular survival of H. pylori in AGS ***: Mir-30 d increases intracellular survival of H. pylori in gastric epithelial cells through inhibition of multiple core proteins in the autophagy pathway.

关键词： mir-30d Helicobacter pylori Autophagy Gene expression Gastric cancer

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A rare case of purulent pericarditis secondary to subdiaphragmatic abscess

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World Journal of Emergency Medicine 2023年第3期14卷 244-246页

作者： Song yang Chao Cui Wen-peng Yin Yu-dong Fu Guang-li Tang xiao-jun yang Guo-feng Qu Zhi-jun Jia Lin-na Cao Ke-song Tang Bao-jian Zhang Xia-lei Du Department of Emergency Medicine Beijing Huairou HospitalBeijing 101499China Department of Emergency Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China

Subdiaphragmatic abscess is the accumulation of pus in the space between the diaphragm and the transverse colon and its mesentery.[1]Subdiaphragmatic abscess is clinically characterized by fever and local *** clinical manifestations are often vague and diverse,and its symptoms and signs together constitute thoracoabdominal syndrome,leading to delayed diagnosis and a high incidence rate and mortality.[2]Subdiaphragmatic abscess is often secondary to acute peritonitis or remote infection with hematogenous *** bacteriological characteristics of these abscesses include aerobic and facultative bacteria,such as Escherichia coli,group D Enterococcus and Staphylococcus aureus,as well as less common anaerobic organisms,such as Bacteroides.

关键词： abscess diagnosis mortality

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Effect of postoperative early enteral nutrition on clinical outcomes and immune function of cholangiocarcinoma patients with malignant obstructive jaundice

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World Journal of Gastroenterology 2020年第46期26卷 7405-7415页

作者： Bing-Qiang Ma Shi-Yong Chen Ze-Bin Jiang Biao Wu Yu He Xin-Xin Wang Yuan Li Peng Gao xiao-jun yang Department of General Surgery Gansu Provincial HospitalLanzhou 730000Gansu ProvinceChina School of Clinical Medicine Ningxia Medical UniversityYinchuan 750000Ningxia Hui Autonomous RegionChina School of Clinical Medicine Gansu University of Chinese MedicineLanzhou 730000Gansu ProvinceChina

BACKGROUND Most cholangiocarcinoma patients with malignant obstructive jaundice(MOJ)have varying degrees of malnutrition and immunodeficiency ***,perioperative nutritional support has important clinical significance in the treatment of *** To investigate the effects of postoperative early enteral nutrition(EEN)on immunity function and clinical outcomes of cholangiocarcinoma patients with *** This prospective clinical study included 60 cholangiocarcinoma patients with MOJ who underwent *** patients were randomly divided into an experimental group and a control group according to the nutrition support *** control group received postoperative total parenteral nutrition(TPN),whereas the experimental group received postoperative EEN and parenteral nutrition(PN;EEN+PN).The clinical outcomes,postoperative immune function,incidences of surgical site infection and bile leakage,intestinal function recovery time,average hospitalization days,and hospitalization expenses of the two groups were assessed on postoperative days(PODs)1,3,and *** The CD3+T,CD4+T,CD8+T,and CD4+T/CD8+T cell count and the immunoglobulin(Ig)G,IgM,and IgA levels in the EEN+PN group were significantly higher than those in the TPN group on PODs 3 and 7(P0.05).The intestinal function recovery time and postoperative hospital stay were shorter(P0.05).CONCLUSION A postoperativ

关键词： Clinical outcomes Early enteral nutrition Immune function Nutritional status Malignant obstructive jaundice Cholangiocarcinoma

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Chinese expert consensus on cytoreductive surgery and hyperthermic intraperitoneal chemotherapy for peritoneal malignancies

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World Journal of Gastroenterology 2016年第30期22卷 6906-6916页

作者： Yan Li Yun-Feng Zhou Han Liang Hua-Qing Wang Ji-Hui Hao Zheng-Gang Zhu De-Seng Wan Lun-Xiu Qin Shu-Zhong Cui Jia-Fu Ji Hui-Mian Xu Shao-Zhong Wei Hong-Bin Xu Tao Suo Shu-jun yang Cong-Hua Xie xiao-jun yang Guo-Liang yang Department of Peritoneal Cancer Surgery Cancer Center of Beijing Shijitan Hospital Affiliated to the Capital Medical University Department of Oncology Zhongnan Hospital of Wuhan University Hubei Cancer Clinical Study Center Cancer Hospital of Tianjin Medical University National Cancer Clinical Study Center Ruijin Hospital Affiliated to Shanghai Jiaotong University Cancer Hospital Affiliated to Sun Yat-Sen University Department of General Surgery Huashan Hospital Affiliated to Fudan University Cancer Hospital Affiliated to Guangzhou Medical University Cancer Hospital Affiliated to Peking University Department of Surgical Oncology the First Hospital Affiliated to China Medical University Hubei Cancer Hospital Beijing Aeronautics Central Hospital Department of General Surgery Zhongshan Hospital Affiliated to Fudan University Cancer Hospital of Henan Province Affiliated to Zhengzhou University

Locoregional spread of abdominopelvic malignant tumors frequently results in peritoneal carcinomatosis(PC). The prognosis of PC patients treated by conventional systemic chemotherapy is poor, with a median survival of < 6 mo. However, over the past three decades, an integrated treatment strategy of cytoreductive surgery(CRS) + hyperthermic intraperitoneal chemotherapy(HIPEC) has been developed by the pioneering oncologists, with proved efficacy and safety in selected patients. Supported by several lines of clinical evidence from phases Ⅰ, Ⅱ and Ⅲ clinical trials, CRS + HIPEC has been regarded as the standard treatment for selected patients with PC in many established cancer centers worldwide. In China, an expert consensus on CRS + HIPEC has been reached by the leading surgical and medical oncologists, under the framework of the China Anti-Cancer Association. This expert consensus has summarized the progress in PC clinical studies and systematically evaluated the CRS + HIPEC procedures in China as well as across the world, so as to lay the foundation for formulating PC treatment guidelines specific to the national conditions of China.

关键词： Expert consensus Peritoneal carcinomatosis Cytoreductive surgery Intraperitoneal hyperthermic chemotherapy Gastric cancer Colorectal cancer Ovarian cancer Peritoneal mesothelioma Pseudomyxoma Peritonei Peritoneal sarcoma

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In vitro and in vivo protective effects of proteoglycan isolated from mycelia of Ganoderma lucidum on carbon tetrachlorideinduced liver injury

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World Journal of Gastroenterology 2006年第9期12卷 1379-1385页

作者： xiao-jun yang Jing Liu Lin-Bai Ye Fan yang Li Ye Jin-Rong Gao Zheng-Hui Wu College of Life Sciences Wuhan University Wuhan 430072 Hubei Province China College of Pharmacy Wuhan University Wuhan 430072 Hubei Province China

AIM： To investigate the possible mechanism of the protective effects of a bioactive fraction, Ganoderma lucidum proteoglycan （GLPG）isolated from Ganoderma luddum mycelia, against carbon tetrachloride-induced liver injury. METHODS： A liver injury model was induced by carbon tetrachloride. Cytotoxicity was measured by MTY assay. The activities of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were determined with an automatic multifunction-biochemical analyzer and the levels of superoxide dismutase （SOD） and TNF-α were determined following the instructions of SOD kit and TNF radioimmunoassay kit. Uver sections were stained with hematoxylin and eosin （H＆E） for histological evaluation and examined under light microscope. RESULTS： We found that GLPG can alleviate the L-02 liver cells injury induced by carbon tetrachloride （CCh） through the measurements of ALT and AST activities and the administration of GLPG to L-02 cells did not display any toxicity. Furthermore, histological analysis of mice liver injury induced by CCh with or without GLPG pretreatment indicated that GLPG can significantly suppress the toxicity induced by CCh in mice liver. We also found that GLPG reduced TNF-α level induced by CCh in the plasma of mice, whereas increased SOD activity in the rat serum. CONCLUSION： GLPG has hepatic protective activity against CCl4 induced injury both in vitro and in vivo. The possible antihepatotoxic mechanisms may be related to the suppression of TNF-α level and the free radical scavenging activity.

关键词： Ganoderma lucidum proteoglycan (GLPG) Carbon tetrachloride (CCl4) Uver injury Hepatic protective activity

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Preliminary study on Salvia miltiorrhiza bung endophytic fungus

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Journal of Pharmaceutical Analysis 2010年第4期22卷 241-246页

作者： Xi-Ying Wei,Ming-Bo Jing,Jin-Cheng Wang,xiao-jun yang College of Life Sciences,Shaanxi Normal University,Xi’an 710062,China College of Life Sciences Shaanxi Normal University Xi'an 710062 China

Objective To select the strains which can produce tanshinone ⅡA like its host plant Salvia miltiorrhiza *** A total of 50 strains of endophytic fungi were isolated from healthy,living and symptomless tissues of Salvia miltiorrhiza bung,among which 29 strains were obtained from the root,14 from the stem,3 from the leaf,3 from the flower and 1 from the *** antimicrobial activities against nine different bacteria,including both Gram-negative and Gram-positive bacteria,were measured by Oxford plate agar diffusion *** Our data showed that all but four strains had significant antibacterial activities on at least one indicator bacterium to some extent,and five strains(DR1,DR4,DR16,DR18 and DF2)manifested quite prominent antibacterial activities against certain pathogenic *** some degree,it might indicate that this endophytic fungus isolated from the tissues of Salvia miltiorrhiza bung has a potential value as a natural antibacterial medicine as *** layer chromatography(TLC)and high-performance liquid chromatography(HPLC)were carried out to test selected strains,both inside and outside of the cell to see if any strain can produce tanshinone Ⅱ*** result showed that extracts from three strains,labeled as DR12(outside cell),DR21(inside cell)and DF3(inside cell),had a component with the same Rf value in TLC assay as that of authentic tanshinone Ⅱ*** extract from DR12(outside cell)and DR21(inside cell)had a peak at retention time identical to that of authentic tanshinone ⅡA in *** The fungi appear to produce the bioactive compound tanshinone ⅡA,and they could be used to produce tanshinone ⅡA by *** provides a new way to synthesize this natural medicine.

关键词： salvia miltiorrhiza bung endophytic fungus antimicrobial activity tanshinone ⅡA

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The coexistence of seven sympatric fulvettas in Ailao Mountains,Ejia Town,Yunnan Province

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Zoological Research 2015年第1期36卷 18-28页

作者： Ji XIA Fei WU Wan-Zhao HU Jian-Ling FANG xiao-jun yang State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyChinese Academy of Sciences University of Chinese Academy of Sciences Shuangbai Ailaoshan Nature Reserve Management Bureau

The coexistence of ecologically similar species sharing sympatric areas is a central issue of community ecology. Niche differentiation is required at least in one dimension to avoid competitive exclusion. From 2012-2014, by adopting the methods of mist-nets and point counts to evaluate spatial niche partitioning and morphological differentiations, we explored the coexistence mechanisms of seven sympatric fulvettas in Ailao Mountains, Ejia town, Yunnan Province, China. The microhabitats of these seven fulvettas were significantly different in elevation, roost site height and vegetation coverage, indicating a spatial niche segregation in different levels. Approximately, 90.30% of the samples were correctly classified by linear discriminant analysis（LDA） with correct rates at 91.20%-100%, except the White-browed fulvetta（Alcippe vinipectus）（65.4%） and the Streak-throated fulvetta（A. cinereiceps）（74.6%）. The seven fulvettas were classified into four guilds based on their specific morphological characters, suggesting that the species in each guild use their unique feeding ways to realize resource partitioning in the overlapped areas. These finding indicate that through multi-dimensional spatial niche segregation and divergence in resource utilizing, the interspecific competition among these seven fulvettas is minimized, whereas, coexistence is promoted.

关键词： coexistence partitioning discriminant correctly utilizing coverage classified morphological niche overlapped

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细胞内锌稳态调控及其在胰腺癌发生发展过程中的作用

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世界华人消化杂志 2017年第18期25卷 1615-1623页

作者：杨晓军甘肃省人民医院普外科甘肃省兰州市730000

锌参与300种以上的细胞生理过程,包括DNA及蛋白合成、酶的活化以及细胞内信号转导过程.同时锌也是许多酶类,如碳酸酐酶、基质金属蛋白酶的关键组分,而这些酶类与缺氧、血管生成、细胞增殖及肿瘤转移密切相关,因此,锌的获取对于恶性肿瘤... 详细信息

锌参与300种以上的细胞生理过程,包括DNA及蛋白合成、酶的活化以及细胞内信号转导过程.同时锌也是许多酶类,如碳酸酐酶、基质金属蛋白酶的关键组分,而这些酶类与缺氧、血管生成、细胞增殖及肿瘤转移密切相关,因此,锌的获取对于恶性肿瘤的生长和进展非常重要.细胞内锌离子的浓度变化受到Zn T1、ZIP4、金属硫蛋白及金属转录因子1等的调控,细胞内锌稳态是多种调控机制参与下锌内流、锌外流和保留之间动态平衡的结果.已有大量研究证实锌稳态调控失衡与胰腺癌的发生和转移有关,因此,锌及锌稳态的调控异常在胰腺癌的发生发展中具有重要作用.

关键词：胰腺癌细胞内锌稳态调控金属硫蛋白金属转录因子1

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The breeding biology of Red-Whiskered Bulbul(Pycnonotus jocosus) in Xishuangbanna, southwest China

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Zoological Research 2015年第4期36卷 233-240页

作者： Huan LI Ming-Xia ZHANG xiao-jun yang Liang-Wei CUI Rui-Chang QUAN The Faculty of Forestry Southwest Forestry University Center for Integrative Conservation Xishuangbanna Tropical Botanical Garden Chinese Academy of Sciences The Faculty of Ecotourism Southwest Forestry University

To fill the gap in breeding biology information about the Red-Whiskered Bulbul （Pycnonotus jocosus）, in 2013, we studied the breeding biology of this species in Xishuangbanna, southwest China. The breeding began from February and continued until early August. The breeding strategy of P. jocosus was more flexible and their nests were only built in cultivated landscapes, whereas, no nest building in native tropical rain forest was found. Small open cup nests were built on 50 different plant species, and at heights ranging from 2.1±0.6 m above the ground （n=102）. The mean clutch size was 2.53±0.51 eggs （n=40） and the mean egg mass was 2.81±0.25 g （n=60）. The average incubation period was 11.1±0.5 days （n=14）, and the average nestling period was 11.0±0.8 days （n=31）. The overall nest success was 34.22%.The hatching and fledging showed either asynchrony or synchrony. Invertebrate food decreased with nestling age, whereas, plant food increased with nestling age. Moreover, distinct parental roles of the parents in nestling period were found. Compared with other passerine species, P. jocosus spent less time in incubating （58%）. The clutch size, incubation and nestling period of the P. jocosus in southwest China were different from those of the R jocosus in India.

关键词： Red-Whiskered Bulbul Breeding biology Incubation Nestling Parental roles

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Preparation and electrochemical property of Cs0.35V2O5/Cu composite material

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Journal of Chemistry and Chemical Engineering 2008年第6期2卷 52-55页

作者： CAO xiao-yu yang jun XIE Ling-ling LIU Xing WANG Hong-yan YAN Xiang-yang School of Chemistry and Chemical Engineering Henan University of Technology Zhengzhou 450001 China

Cs0.35V2O5 was successfully synthesized as cathode material for lithium secondary battery by the rheological phase reaction method from Cs2CO3 and NH4VO3. The Cs0.35V2O5/Cu composite material was prepared by the displacement reaction in CuSO4 solution using zinc powder as a reductant. The structure and electrochemical property of the so-prepared powders were characterized by means of XRD （powder X-ray diffraction） and the galvanostatic discharge-charge techniques. The results show that the electrochemical property of Cs0.35V2O5/Cu composite material is significantly improved compared to the bulk Cs0.35V2O5 material. The Cs0.35V2O5/Cu composite material exhibits the first discharge capacity as high as 164.3 mAh.g -1 in the range of 4.2-1.8V at a current rate of 10 mA.g-1 and remains at a stable discharge capacity of about 110 mAh.g-1 within 40 cycles.

关键词： lithium secondary batteries cathode material Cs0.35V2O5/Cu composite material electrochemical property

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