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Semaphorin 7A impairs barrier function in cultured human corneal epithelial cells in a manner dependent on nuclear factor-kappa B

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International Journal of Ophthalmology(English edition) 2024年第3期17卷 444-453页

作者： Cheng-Cheng Yang Xiu-Xia Yang xiao-jing zhao Heng Wang Zi-Han Guo Kai Jin Yang Liu Bin-Hui Li Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen UniversityZhuhai 519000Guangdong ProvinceChina Department of Ophthalmology Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical UniversityHangzhou 310006Zhejiang ProvinceChina Eye Institute of Xiamen University Fujian Provincial Key Laboratory of Ophthalmology and Visual ScienceSchool of MedicineXiamen UniversityXiamen 361005Fujian ProvinceChina

●AIM:To evaluate the role of semaphorin 7A(Sema7A)and its associated regulatory mechanisms in modulating the barrier function of cultured human corneal epithelial cells(HCEs).●METHODS:Barrier models of HCEs were treated with recombinant human Sema7A at concentrations of 0,125,250,or 500 ng/mL for 24,48,or 72h in vitro.Transepithelial electrical resistance(TEER)as well as Dextran-fluorescein isothiocyanate(FITC)permeability assays were conducted to assess barrier function.To quantify tight junctions(TJs)such as occludin and zonula occludens-1(ZO-1)at the mRNA level,reverse transcriptionpolymerase chain reaction(RT-PCR)analysis was performed.Immunoblotting was used to examine the activity of the nuclear factor-kappa B(NF-κB)signaling pathway and the production of TJs proteins.Immunofluorescence analyses were employed to localize the TJs.Enzyme-linked immunosorbent assay(ELISA)and RT-PCR were utilized to observe changes in interleukin(IL)-1βlevels.To investigate the role of NF-κB signaling activation and IL^(-1)βin Sema7A’s anti-barrier mechanism,we employed 0.1μmol/L IκB kinase 2(IKK2)inhibitor IV or 500 ng/mL IL^(-1)receptor(IL-1R)antagonist.●RESULTS:Treatment with Sema7A resulted in decreased TEER and increased permeability of Dextran-FITC in HCEs through down-regulating mRNA and protein levels of TJs in a time-and dose-dependent manner,as well as altering the localization of TJs.Furthermore,Sema7A stimulated the activation of inhibitor of kappa B alpha(IκBα)and expression of IL-1β.The anti-barrier function of Sema7A was significantly suppressed by treatment with IKK2 inhibitor IV or IL-1R antagonists.●CONCLUSION:Sema7A disrupts barrier function through its influence on NF-κB-mediated expression of TJ proteins,as well as the expression of IL-1β.These findings suggest that Sema7A could be a potential therapeutic target for the diseases in corneal epithelium.

关键词： human corneal epithelial barrier function transepithelial electrical resistance zonula occludens-1 occludin nuclear factor-kappa B

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Mantle cell lymphoma with endobronchial involvement:A case report

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World Journal of Clinical Cases 2022年第8期10卷 2604-2609页

作者： Yi-Zong Ding Dao-Qiang Tang xiao-jing zhao Department of Thoracic Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Department of Pathology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China

BACKGROUND Mantle cell lymphoma(MCL)is a subtype of Non-Hodgkin’s lymphoma(NHL).MCL frequently affects extranodal sites while endobronchial involvement is uncommon.Only 5 cases of MCL with endobronchial involvement have been previously reported.CASE SUMMARY A 56-year-old male patient arrived at the hospital complaining of a dry cough.A mass in the right upper lobe of the lung was revealed in Chest computed tomography(CT).Right lung hilar and mediastinal lymphadenopathies were also found by CT scan.The patient was diagnosed with central-type lung cancer with multiple lymph node metastases after positron emission tomography(PET)CT scan examination.The fiber optic bronchoscope examination revealed diffuse neoplasm infiltration in the inlet of the right up lobar bronchus.The patient was finally diagnosed with MCL based on the bronchoscopy and mediastinoscopy biopsy results.CONCLUSION MCL could masquerade as central type lung cancer.An endobronchial biopsy examination is necessary for the early diagnosis of MCL.

关键词： Mantle cell lymphoma Endobronchial involvement Central type lung cancer Endobronchial biopsy Bronchoscopy Mediastinoscopy Case report

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Fecal microbial dysbiosis in Chinese patients with inflammatory bowel disease

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World Journal of Gastroenterology 2018年第13期24卷 1464-1477页

作者： Hai-Qin Ma Ting-Ting Yu xiao-jing zhao Yi Zhang Hong-Jie Zhang Department of Gastroenterology First Affiliated Hospital of Nanjing Medical University

AIM To analyze the alterations of fecal microbiota in Chinese patients with inflammatory bowel disease(IBD).METHODS Fecal samples from 15 patients with Crohn's disease(CD)(11 active CD, 4 inactive CD), 14 patients with active ulcerative colitis(UC) and 13 healthy individuals were collected and subjected to 16 S ribosomal DNA(rDNA) gene sequencing. The V4 hypervariable regions of 16 S rDNA gene were amplified from all samples and sequenced by the Illumina Mi Seq platform. Quality control and operational taxonomic units classification of reads were calculated with QIIME software. Alpha diversity and beta diversity were displayed with R software.RESULTS Community richness(chao) and microbial structure in both CD and UC were significantly different from those in normal controls. At the phyla level, analysis of the microbial compositions revealed a significantly greater abundance of Proteobacteria in IBD as compared to that in controls. At the genera level, 8 genera in CD and 23 genera in UC(in particular, the Escherichia genus) showed significantly greater abundance as compared to that in normal controls. The relative abundance of Bacteroidetes in the active CD group was markedly lower than that in the inactive CD group. The abundance of Proteobacteria in patients with active CD was nominally higher than that in patients with inactive CD; however, the difference was not statistically significant after correction. Furthermore, the relative abundance of Bacteroidetes showed a negative correlation with the CD activity index scores.CONCLUSION Our study profiles specific characteristics and microbial dysbiosis in the gut of Chinese patients with IBD. Bacteroidetes may have a negative impact on inflammatory development.

关键词： Crohn’s disease Ulcerative colitis Chinese Microbial dysbiosis 16S ribosomal DNA

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Association of COL1A1 polymorphism with high myopia: a Meta-analysis

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International Journal of Ophthalmology(English edition) 2016年第4期9卷 604-609页

作者： Guang-Ming Jin xiao-jing zhao Ai-Ming Chen Yong-Xing Chen Qin Li State Key Laboratory of Ophthalmology Zhongshan Ophthalmic Center Sun Yat-sen University Department of Ophthalmology the Fifth Affiliated Hospital of Sun Yat-sen University Department of Pharmacy the Fifth Affiliated Hospital of Sun Yat-sen University Department of Otorhinolaryngology Jiangmen Central Hospital

AIM： To investigate the association between collagen type I alpha 1（COL1A1） gene and high myopia.· METHODS： In this Meta-analysis, we examined 5published case-control studies that involved 1942 high myopia cases and 2929 healthy controls to assess the association between the COL1A1 rs2075555 polymorphism and high myopia risk. We calculated the pooled odds ratios（ORs） of COL1A1 rs2075555 polymorphism in high myopia cases vs healthy controls to evaluate the strength of the association.·RESULTS： Overall, there was no significant difference both in the genotype and allele distributions of COL1A1rs2075555 polymorphism between high myopia cases and healthy controls： CC vs AA OR =1.10, 95% confidence interval（CI）=0.76-1.58; AC vs AA OR=0.98, 95%CI 0.80-1.20; CC/AC vs AA/OR=1.01, 95%CI 0.84-1.22; CC vs AC/AA OR =1.06, 95% CI =0.93-1.20; C vs A OR =1.06, 95% CI0.91-1.23）. In addition, in the stratified analyses by ethnicity, no significant associations were found in any genetic model both in European and Asia cohorts.·CONCLUSION： Our results indicate that the COL1A1rs2075555 polymorphism may not affect susceptibility to high myopia.

关键词： collagen type I alpha 1 polymorphism high myopia Meta-analysis

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Tranilast inhibits TGF-β-induced collagen gel contraction mediated by human corneal fibroblasts

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International Journal of Ophthalmology(English edition) 2018年第8期11卷 1247-1252页

作者： Ye Liu xiao-jing zhao xiao-Shuo Zheng Hui Zheng Lei Liu Ling-Bin Meng Qin Li Yang Liu Department of Pathology the Fifth Affiliated HospitalSun Yat-sen University Department of Ophthalmology the Fifth Affiliated HospitalSun Yat-sen University Department of Ophthalmology the First Hospital of Jilin University Department of Internal Medicine Florida Hospital

AIM： To determine if tranilast affects human corneal fibroblast（HCFs） contraction.METHODS： HCFs cultured in a three-dimensional type I collagen gel were treated with or without transforming growth factor beta（TGF-β） or tranilast. Gel diameter was measured as an indicator for collagen contraction. Immunoblot was performed to evaluate myosin light chain（MLC） and paxillin phosphorylation. Confocal microscopy was employed to examine the focal adhesions and actin stress fiber formation. Immunoblot analysis and gelatin zymography were performed to detect tissue inhibitors of metalloproteinases and matrix metalloproteinases（MMPs） in supernatant.RESULTS： The inhibitory effect of tranilast on HCFsmediated collagen gel contraction induced by TGF-β was dose-dependent. The significant effect of tranilast was started from 100 μmol/L and maximized at 300 μmol/L. The peak effect of 300 μmol/L tranilast also relied on the duration of treatment, which showed statistical significance from day 2. TGF-β-induced paxillin and MLC phosphorylation, stress fiber formation, focal adhesions, and MMP-1, MMP-2, and MMP-3 secretion in HCFs were also inhibited by tranilast.CONCLUSION： Tranilast suppresses the HCFs-cultured collagen gel contraction induced by TGF-β. It attenuates actin stress fibers formation, focal adhesions, and the secretion of MMPs, with these actions likely contributing to the inhibitory effect on HCF contractility. By attenuating the contractility of corneal fibroblasts, tranilast treatment may inhibit corneal scarring.

关键词： tranilast transforming growth factor beta corneal fibroblast corneal wound healing

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Efficacy and economic benefits of a modified Valsalva maneuver in patients with paroxysmal supraventricular tachycardia

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World Journal of Clinical Cases 2020年第23期8卷 5999-6008页

作者： Wei Wang Teng-Fei Jiang Wei-Zhong Han Lin Jin xiao-jing zhao Ying Guo Internal Medicine-Cardiovascular Department Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina Department of Nursing The First Affiliated Hospital of Shandong First Medical University Department of Nursing Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021Shandong ProvinceChina

BACKGROUND A modified Valsalva maneuver(VM)has been suggested to be superior to the standard VM for conversion of paroxysmal supraventricular tachycardia(PSVT).AIM To evaluate the efficacy and economic benefits of a modified VM in Chinese patients.METHODS Patients with PSVT admitted to our center between October 2017 and September 2019 were randomly assigned to the modified and standard VM groups.Conversion via VM was performed up to three times.The primary outcome of the study was the success rate of PSVT conversion to sinus rhythm.The secondary outcomes included the incidence of adverse events,economic cost during the visit,and the degree of patient acceptance of the treatment.RESULTS Overall,361 patients were enrolled,with 180 allocated to the modified VM group and 181 to the standard VM group.Baseline characteristics were well matched in the groups.Overall,the modified VM group had higher success rates of PSVT conversion after single(47.78%vs 15.38%,P0.05).Moreover,the economic cost of the clinic visit was significantly lower for the modified VM group than for the standard VM group(P<0.05).CONCLUSION The modified VM may confer both therapeutic and economic benefits as compared with the standard VM for conversion of PSVT.

关键词： Paroxysmal supraventricular tachycardia Modified Valsalva maneuver Costeffective analysis

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The effects of surface ligands and counter cations on the stability of anionic thiolated M_(12)Ag_(32)(M=Au,Ag) nanoclusters

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Chinese Chemical Letters 2014年第6期25卷 839-843页

作者： Xin Zhang Hua-Yan Yang xiao-jing zhao Yu Wang Nan-Feng Zheng State Key Laboratory for Physical Chemistry of Solid Surfaces Xiamen University Department of Chemistry College of Chemistry and Chemical EngineeringXiamen University

The stabilities of [M12Ag32（SR）30]4- （M = Ag, Au and SR = SPhF2, SPhCF3, SPhF） clusters having the same structure but different surface ligands or counter cations were systematically studied. It was clearly revealed that a subtle structural change in the surface ligands or counter cations could significantly alter the overall stability of [M12Ag32（SR）30]4 although they all had an electronic structure of 18-electron superatom shell closure. SPhF2 was found as a better surface ligand than SPhCF3 or SPhF to stabilize [M12Ag32（SR）30]4-. And the use of more bulky [（PPhj）2N]＋ as the counter cations was revealed to be more deleterious to the overall stability of [M12Ag32（SR）30]4- clusters than PPh4＋. [Au12Ag32（SR）30]4- was much more stable than [Ag44（SR）30]4 with the same surface ligands and counter cations. An exceptional stability was observed on （PPh4）4[Au12Ag32（SPhF2）30] which was stable in DMF for more than 8 days in air at 80 ℃. More research efforts are still needed to deeply understand why a small structural change could result in a significant change in the stability of noble metal nanoclusters.

关键词： Nanoclusters Stability Ligand effect Noble metal

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Oxidative intramolecular coupling of 2,3-disubstituted phenyl acrylic acids and derivatives promoted by di-tert-butylperoxide

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Chinese Chemical Letters 2014年第2期25卷 348-350页

作者： De-Rong Ji Hua Yang xiao-jing zhao Hao Yang Yang-zhao Liu Dai-Hui Liao Chun Feng Cheng-Gang Zhang College of Chemistry and Materials Sichuan Normal University

Polymethoxy-substituted phenanthrene-9-carboxylic acids or their methylate are key intermediates for the synthesis of tylophora alkaloids and their analogs. An intramolecular oxidative coupling reaction of unfunctionalized 2,3-disubstituted phenyl acrylic acids and derivatives promoted by di-tert-butylper- oxide gave above intermediates in high yields. The mild reaction conditions and easy purification procedures of this method provide a new approach for the synthesis of phenanthrenes.

关键词： Polymethoxylphenanthrene Cross dehydrogenative coupling （cdc） Radical cation Intramolecular oxidative coupling

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β-receptor blocker influences return of spontaneous circulation and chemical examination in rats during cardiopulmonary resuscitation

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第6期14卷 505-510页

作者： xiao-jing zhao Zhuo PEN Ping LI Er-xiu CHEN Jian LIU Yan-xia GAO Yun-xia REN Li-jun LI Department of Emergency Medicine the Second Affiliated Hospital of Medical CollegeXi'an Jiaotong University Mechanical Energy and Experimental Center of Medical CollegeXi'an Jiaotong University

Objective:We investigated the influence of β-receptor blocker metoprolol on return of spontaneous circulation(ROSC) during cardiopulmonary resuscitation(CPR) in rats with induced myocardial infarction(MI).Methods:Male Sprague-Dawley rats were randomly divided into three groups:the sham-operated group,the MI group without metoprolol,which was fed the vehicle,and the MI+metoprolol group receiving intragastric metoprolol.Each group was further divided randomly into three subgroups,depending on the dosage of epinephrine administered during subsequent CPR applied after the induction of asphyxial cardiac arrest.Results:The ROSC rate was significantly decreased in the low dose subgroup of MI group,unchanged in the medium dose subgroup of MI group,and significantly decreased in the high dose subgroup of MI group,compared with the same dose subgroup of sham-operated group.MI+metoprolol group had a lower ROSC rate than MI group in the medium dose subgroup,and a higher ROSC rate than MI group in the high dose subgroup.There was no difference in blood K+ values of successful rats between MI group and MI+metoprolol group.The rats with successful CPR had lower blood K+ values than rats with unsuccessful CPR in each of the three treatment groups.Conclusions:Metoprolol administered to MI rats over a long period significantly improved ROSC rates under an appropriate dose of epinephrine during CPR.An increasing high blood K+ value would attenuate the rate of a successful CPR.

关键词： β-receptor blocker Cardiopulmonary resuscitation Return of spontaneous circulation Epinephrine Chemical examination

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Characterization of a Chinese KCNQ1 mutation （R259H） that shortens repolarization and causes short QT syndrome 2

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Journal of Geriatric Cardiology 2015年第4期12卷 394-401页

作者： Zhi-Juan WU Yun HUANG Yi-Cheng FU xiao-jing zhao Chao ZHU Yu ZHANG Bin XU Qing-Lei ZHU Yang LI Department of Cardiology Chinese PLA General Hospital 28 Fuxing Road Beijing China Department of Gerontology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

ObjectivesTo 评估在一个 KCNQ1 变化， R259H，和短 QT 症候群(SQTS ) 之间并且到的协会探索位于他们的 association.MethodsWe 下面的机制执行了在 25 probands 和他们的家庭成员(63 个病人) 的 SQTS 基因的基因屏蔽的 electrophysiol... 详细信息

ObjectivesTo 评估在一个 KCNQ1 变化， R259H，和短 QT 症候群(SQTS ) 之间并且到的协会探索位于他们的 association.MethodsWe 下面的机制执行了在 25 probands 和他们的家庭成员(63 个病人) 的 SQTS 基因的基因屏蔽的 electrophysiological。我们使用了屏蔽编码贡献室的行动潜力的极化的离子隧道的候选人基因的 exons 和 intron-exon 边界的直接定序，包括 KCNQ1， KCNH2， KCNE1， KCNE2， KCNJ2， CACNA1c， CACNB2b 和 CACNA2D1。在屏蔽的 25 SQTS probands 之一，我们发现了一个 KCNQ1 变化， R259H。我们克隆 R259H 并且短暂地在 HEK-293 房间表示了它；然后，水流用整个房间补丁被记录夹钳显著地显示出的 techniques.ResultsR259H-KCNQ1 增加了当前的密度，它在在 1 s 的动摇脉搏以后是近似比野类型(WT ) 的大的 3 褶层。激活和 inactivation 的稳定的州的电压依赖没显示出 WT 和 R259H 变化之间的重要差别(P >；0.05 ) 而释放的时间常数显著地以测试潜力与 WT 相比在异种被延长，它显示 R259H 的释放比 WT 的显著地慢。这些结果建议 R259H 变化罐头有效地增加慢慢地激活的推迟的整流器钾电流(我 K ) 在分阶段执行 3 心脏的行动潜力，它可以是 QT 间隔 shortening.ConclusionsR259H 的一个很少发生的原因是为 SQTS2 负责的 KCNQ1 隧道的一个 gain-of-function 变化。这是 R259H 变化在中国人民被检测的第一次。

关键词：突变特性候选基因综合征中国人 QT 失活动力学延迟整流钾电流动作电位

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