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MMP-1 Over-expression Promotes Malignancy and Stem-Like Properties of Human Osteosarcoma MG-63 Cells In Vitro

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Current Medical Science 2018年第5期38卷 809-817页

作者： Man-li TANG Xiang-jun BAI Yong LI xiao-jing dai Fan YANG Department of Traumatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Orthopaedic Surgery McGovern Medical Schoolthe University of Texas Health Science Center at Houston (UTHealth)Houston77035USA Center for Stem Cell and Regenerative Medicine UTHealthHouston 77035USA Center for Regenerative Sports Medicine Steadman Philippon Research InstituteFail 81657USA Department of Pharmacology and Toxicology College of Pharmacythe University of HoustonHouston 77555USA

Osteosarcoma is the most common primary malignant bone tumor in childhood,and it maintains a high level of *** metalloproteinase-1(MMP-1)was found to contribute to cancer *** present study was to investigate the in vitro effects of MMP-1 over-expression on the proliferation,invasion,metastasis and stem-like properties of osteosarcoma MG-63 *** MG-63 cells were cultured and had a full length MMP-1 cDNA inserted by the tentiviral vector (MG-63^MMP-1+).MG-63 negative control and MG-63 blank control groups were established as ***-1 expression was detected in MG-63^MMP-1+,MG-63 negative control and MG-63 blank control cells using qPCR,Western blotting and immunofluorescence after 24h of culture. The cell proliferation assay was performed with a camera attached to a bioreactor,which was programmed to photograph five regions of each well every 10 min over a period of 48 *** cell invasion assay was conducted with Matrigel to assess the invasive potential,of MG-63 cells over 24h,the qPCR analysis to measure stem cell markers,including Oct4, Sox-2,Nanog,and Pax-7,and Western blot analysis to detect invasive and metastatic potential markers TIMP-1,VEGF and BMP2/4,after 24h of *** was used to investigate the presence of the stem cell marker Pax-7 after 24-h culture. The results showed that over-expression of MMP-1 after transfection could significantly increase minor cell proliferation and invasion (P<0.05,MG-63^MMP-1+ versus controls).Pax-7 was highly expressed in MG-63^MMP-1+ cells,with no significant changes of Oct-4,Sox-2, and Nanog observed (P<0.05).MG-63^MMP-1+ cells showed higher expression of VEGF and BMP 2/4 proteins and lower expression of TIMP-1 protein than controls (P<0.05).It was concluded that MMP-1 over-expression in MG-63 cells contributed to the proliferation, invasion,metastasis and stem-like properties of osteosarcoma *** studies should focus on in vivo effects of MMP-1 over-expression an

关键词： osteosarcoma MG-63 cells matrix metalloproteinase-1 proliferation invasion metastasis

Bismuth-based materials for rechargeable aqueous batteries and water desalination

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Rare Metals 2022年第1期41卷 287-303页

作者： xiao-jing dai Xin-Xin Niu Wang-Qin Fu Dong Zheng Wen-Xian Liu Wen-Hui Shi Jian-Wei Nai Fang-Fang Wu Xie-Hong Cao School of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014China Center for Membrane Separation and Water Science&Technology College of Chemical EngineeringZhejiang University of TechnologyHangzhou 310014China

Aqueous batteries and seawater desalination have received considerable attention in recent years due to their merits as high safety,environmental friendliness and ***,the scarcity of highly match electrode materials hinders their *** exploration of high performance and low cost electrode materials is crucial for their potential ***(Bi),with high energy density and low redox potential,shows perspective in the field of aqueous batteries and seawater desalination,and significant progress has been achieved in the past *** this review,the unique properties and synthetic methods of Bi-based electrodes,as well as their applications are comprehensively summarized and *** commonly used preparation methods of Bibased electrodes,including hydrothermal method,electrodeposition method,etc.,are ***,the applications of the Bi-based composites in aqueous batteries,such as Ni//Bi batteries and water desalination,are ***,the challenges and future research direction of Bi-based materials are proposed.

关键词： Bismuth Aqueous batteries Desalination Capacitive deionization Derivatives

Highly conductive and stable iodine doped argyrodite electrolyte for all-solid-state lithium batteries

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Journal of Energy Chemistry 2025年第1期100卷 50-58页

作者： Gaozhan Liu jing Zhang jing Yang jinghui Chen Xingyue xiao Xiayin Yao Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences

Lithium argyrodites with high ionic conductivity and low cost are considered as one of the most prospective solid electrolytes for all-solid-state lithium ***,the poor chemical stability and compatibility with lithium metal limit their ***,Li5.4PS4.4Cl1.4I0.2solid electrolyte with high ionic conductivity of 11.49 m S cm-1and improved chemical stability is synthesized by iodine *** ultra-thin Li5.4PS4.4Cl1.4I0.2membrane with thickness of 10μm can be obtained by wet coating process,exhibiting a high ionic conductivity of 2.09 mS cm-1and low areal resistance of 1.17Ω***,iodine doping could in-situ form LiI at the lithium/electrolyte interface and improve the critical current density of Li5.4PS4.4Cl1.6from 0.8 to 1.35 mA *** resultant LiCoO2/Li5.4PS4.4Cl1.4I0.2/Li battery shows excellent cycling stability at 1 C,with a reversible specific capacity of 110.1 mA h g-1and a retention of 80.5% after 1000 *** addition,the assembled LiCoO2/Li5.4PS4.4Cl1.4I0.2membrane/Li pouch cell delivers an initial discharge capacity of 110.4 mA h g-1and 80.5% capacity retention after 100 cycles.

关键词： Argyrodite electrolytes Iodine doping Ionic conductivity Stability All-solid-state lithium battery

miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong jing-jing Hu Yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

Effects of the extrusion parameters on microstructure,texture and room temperature mechanical properties of extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 136-146页

作者： Chenjin Zhang Guangyu Yang Lei xiao Zhiyong Kan jing Guo Qiang Li Wanqi Jie State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi’an 710072China College of Materials Science and Engineering Fuzhou UniversityFuzhou 350108China College of Chemistry and Environmental Science Qujing Normal UniversityQujing 655011China

Microstructure,texture,and mechanical properties of the extruded Mg-2.49Nd-1.82Gd-0.2Zn-0.2Zr alloy were investigated at different extrusion temperatures(260 and 320℃),extrusion ratios(10:1,15:1,and 30:1),and extrusion speeds(3 and 6 mm/s).The experimental results exhibited that the grain sizes after extrusion were much finer than that of the homogenized alloy,and the second phase showed streamline distribution along the extrusion direction(ED).With extrusion temperature increased from 260 to 320℃,the microstructure,texture,and mechanical properties of alloys changed *** dynamic recrystallization(DRX)degree and grain sizes enhanced as the extrusion ratio increased from 10:1 to 30:1,and the strength gradually decreased but elongation(EL)*** the extrusion speed increased from 3 to 6 mm/s,the grain sizes and DRX degree increased significantly,and the samples presented the typical-rare-earth(RE)*** alloy extruded at 260℃ with extrusion ratio of 10:1 and extrusion speed of 3 mm/s showed the tensile yield strength(TYS)of 213 MPa and EL of 30.6%.After quantitatively analyzing the contribution of strengthening mechanisms,it was found that the grain boundary strengthening and dislocation strengthening played major roles among strengthening *** results provide some guidelines for enlarging the industrial application of extruded Mg-RE alloy.

关键词： Mg-rare earth alloys extrusion temperature extrusion ratio extrusion speed strengthening mechanisms

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野百合根系水浸液对几种花卉的化感效应

山地农业生物学报 2016年第5期35卷 13-17页

作者：胡小京代玉苹贵州大学农学院贵州贵阳550025

以贵州野百合(Lilium brownii)根系为供试材料,研究不同浓度野百合根系水浸液对紫罗兰、金鱼草、石竹等受试花卉种子萌发及幼苗生长的化感效应。结果表明:浓度为0.025 g/L^0.2 g/L的野百合根系水浸液对3种受试花卉种子发芽率及发芽指数... 详细信息

以贵州野百合(Lilium brownii)根系为供试材料,研究不同浓度野百合根系水浸液对紫罗兰、金鱼草、石竹等受试花卉种子萌发及幼苗生长的化感效应。结果表明:浓度为0.025 g/L^0.2 g/L的野百合根系水浸液对3种受试花卉种子发芽率及发芽指数产生抑制作用,且随水浸液浓度的提高抑制作用明显增强。水浸液对3种受试花卉幼苗的株高、叶数、根长、侧根数、地上部鲜重及根鲜重的抑制作用亦随浓度的升高而增强。因此,紫罗兰、金鱼草、石竹等受试花卉均不适合作为野百合的后茬作物。

关键词：野百合水浸液化感作用

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目标真温及光谱发射率智能识别法

上海交通大学学报 2001年第9期35卷 1355-1357页

作者：孙晓刚戴景民丛大成褚载祥哈尔滨工业大学自动化测试与控制系哈尔滨150001

基于神经网络技术 ,提出一种可以取消发射率假设模型的方法 .实验结果表明 ,基于神经网络技术的二次辨识方法可以自动识别目标真温及光谱发射率。

关键词：多光谱辐射测温真温光谱发射率神经网络最小二乘法智能识别法二次辨识方法

MET receptor tyrosine kinase promotes the generation of functional synapses in adult cortical circuits

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Neural Regeneration Research 2025年第5期20卷 1431-1444页

作者： Yuehua Cui xiaokuang Ma jing Wei Chang Chen Neha Shakir Hitesch Guirram Zhiyu dai Trent Anderson Deveroux Ferguson Shenfeng Qiu Basic Medical Sciences University of Arizona College of Medicine-PhoenixPhoenixAZUSA Department of Medicine University of Arizona College of Medicine-PhoenixPhoenixAZUSA

Loss of synapse and functional connectivity in brain circuits is associated with aging and neurodegeneration,however,few molecular mechanisms are known to intrinsically promote synaptogenesis or enhance synapse *** have previously shown that MET receptor tyrosine kinase in the developing cortical circuits promotes dendritic growth and dendritic spine *** investigate whether enhancing MET in adult cortex has synapse regenerating potential,we created a knockin mouse line,in which the human MET gene expression and signaling can be turned on in adult(10–12 months)cortical neurons through doxycycline-containing *** found that similar to the developing brain,turning on MET signaling in the adult cortex activates small GTPases and increases spine density in prefrontal projection *** findings are further corroborated by increased synaptic activity and transient generation of immature silent *** MET signaling resulted in an increasedα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid/N-methyl-Daspartate(AMPA/NMDA)receptor current ratio,indicative of enhanced synaptic function and *** data reveal that enhancing MET signaling could be an interventional approach to promote synaptogenesis and preserve functional connectivity in the adult *** findings may have implications for regenerative therapy in aging and neurodegeneration conditions.

关键词： aging circuit connectivity cortical circuits molecular mechanisms neural regeneration neurodegeneration synapses

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桑葚的化学成分和功效作用研究进展

吉林工程技术师范学院学报 2017年第9期33卷 85-87页

作者：谢小花陈静安晓婷戴缘缘刘静滁州职业技术学院食品与环境工程系安徽滁州239000 安徽省凤阳县西泉镇考城数学教研室安徽凤阳233100

桑葚含有丰富的营养成分和功效成分,具有多种生理功效,是药食两用的食品,近年来引起了国内外营养学家、药学专家学者的关注。本文通过对桑葚研究文献的回顾,综述桑葚果实所含的化学成分和功能成分,并简述桑葚的临床及药用价值。

关键词：桑葚化学成分功能成功药用价值