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Integrin-linked kinase in gastric cancer cell attachment,invasion and tumor growth

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World Journal of Gastroenterology 2011年第30期17卷 3487-3496页

作者： Gang Zhao Li-Li Guo Jing-Yong Xu hua Yang Mei-Xiong huang Gang xiao Department of General Surgery Beijing Hospital Peking University 100730 Beijing China Department of Ophthalmology People＇s Hospital Peking University 100044 Beijing China

AIM： To investigate the effects of integrin-linked kinase （ILK） on gastric cancer cells both in vitro and in vivo. METHODS： ILK small interfering RNA （siRNA） was transfected into human gastric cancer BGC-823 cells and ILK expression was monitored by real-time quan- titative polymerase chain reaction, Western blotting analysis and immunocytochemistry. Cell attachment, proliferation, invasion, microfilament dynamics and the secretion of vascular endothelial growth factor （VEGF） were also measured. Gastric cancer cells treated with ILK siRNA were subcutaneously transplanted into nude mice and tumor growth was assessed. RESULTS： Both ILK mRNA and protein levels were significantly down-regulated by ILK siRNA in human gastric cancer cells. This significantly inhibited cell attachment, proliferation and invasion. The knockdown of ILK also disturbed F-actin assembly and reduced VEGF secretion in conditioned medium by 40% （P 〈 0.05）. Four weeks after injection of ILK siRNA-transfected gastric cancer cells into nude mice, tumor volume and weight were significantly reduced compared with that of tumors induced by cells treated with non-silencing siRNA or by untreated cells （P 〈 0.05）. CONCLUSION： Targeting ILK with siRNA suppresses the growth of gastric cancer cells both in v/tro and /n vivo. ILK plays an important role in gastric cancer progression.

关键词： Gastric cancer Integrin-linked kinase Smallinterfering RNA Cell attachment Cell proliferation Cellinvasion Cell microfilament dynamics Vascular endo-thelial growth factor Nude mice

Pathological and MR-DWI study of the acute hepatic injury model after stem cell transplantation

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World Journal of Gastroenterology 2011年第23期17卷 2821-2828页

作者： Quan-Liang Shang En-hua xiao Qi-Chang Zhou Jian-Guang Luo Hai-Jun Wu Department of Radiology Second Xiangya HospitalCentral South University Changsha 410011 Hunan ProvinceChina Department of Ultrasound Second XiangyaHospital Central South University Changsha 410011 HunanProvince China

AIM： To investigate apparent diffusion coefficient （ADC） values as an indication of reconditioning of acute hepatic injury （AHI） after allogeneic mononuclear bone marrow cell （MBMC） transplantation. METHODS： Three groups were used in our study： a cell transplantation group （n = 21）, transplantation control group （n = 21） and normal control group （n = 10）. AHI model rabbits in the cell transplantation group were injected with 5 mL of MBMC suspension at multiple sites in the liver and the transplantation controls were injected with 5 mL D-Hanks solution. At the end of the 1st, 2nd and 4th wk, 7 rabbits were randomly selected from the cell transplantation group and trans- plantation control group for magnetic resonance diffu- sion-weighted imaging （MR-DWI） and measurement of the mean ADC values of injured livers. After MR-DWI examination, the rabbits were sacrificed and the livers subjected to pathological examination. Ten healthy rab- bits from the normal control group were used for MR- DWI examination and measurement of the mean ADC value of normal liver. RESULTS： At all time points, the liver pathological scores from the cell transplantation group were significantly lower than those in the transplantation control group （27.14± 1.46 vs 69.29± 6.16, 22.29 ± 2.29 vs 57.00± 1.53, 19.00 ± 2.31 vs 51.86 ± 6.04, P = 0.000）. The mean ADC values of the cell transplantation group were significantly higher than the transplantation con- trol group （（1.07± 0.07） ×10^-3 mm2/s vs （0.69 ± 0.05） ×10^-3 mm2/s, （1.41± 0.04） ×10^-3 mm2/s vs （0.84± 0.03） ×10^-3 mm2/s, （1.68 ± 0.04） ×10^-3 mm2/s vs （0.86± 0.04） ×10^-3 mm2/s, P = 0.000）. The pathological scores of the cell transplantation group and transplantation control group gradually decreased. However, their mean ADC values gradually increased to near that of the normal control. At the end of the 1st wk, the mean ADC values of the cell transplantation group and transplantation control group were significantly lower than those of the

关键词： Stem cells Transplantation Hepatic injury Magnetic resonance imaging Diffusion weighted imaging

Effects of nicorandil on myocardial infarct size in patients with ST-segment elevation myocardial infarction undergoing primary percutaneous coronary intervention:study design and protocol for the randomized controlled trial

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Journal of Geriatric Cardiology 2020年第8期17卷 519-524页

作者： xiao-Si JIANG Li-Chao TIAN Zi-Chao JIANG Yu-Ting zou Ping LI Xin-Chun YANG Xi SU Jin-Wen TIAN Bei SHI Zong-Zhuang LI Yong-Jun LI Ren-Qiang YANG Geng QIAN Yun-Dai CHEN Department of Cardiology the First Medical Center of Chinese PLA General HospitalBeijingChina The School of Medicine Nankai UniversityTianjinChina Department of Cardiology the First People's Hospital of YunlinGuangxiChina Department of Cardiology Beijing Chaoyang HospitalCapital Medical UniversityBeijingChina Department of Cardiology Wuhan Asia Heart HospitalWuhanChina Department of Cardiology Hainan Hospital of PLA General HospitalHainanChina Department of Cardiology Affiliated Hospital of Zunyi Medical CollegeGuizhouChina Department of Cardiology Guizhou Provincial People's HospitalGuizhouChina Department of Cardiology the Second Hospital of Hebei Medical UniversityHebeiChina Department of Cardiology the Second Affiliated Hospital of Nanchang UniversityJiangxiChina

Previous studies have shown that nicorandil has a protective effect on ***,there is no study to investigate whether perioperative intravenous nicorandil can further reduce the myocardial infarct size in patients with ST-segment elevation myocardial infarction(STEMI)compared to the current standard of percutaneous coronary intervention(PCI)*** CHANGE(China-Administration of Nicorandil Group)study is a multicenter,prospective,randomized,double-blind and parallel-controlled clinical study of STEMI patients undergoing primary PCI in China,aiming to evaluate the efficacy and safety of intravenous nicorandil in ameliorating the myocardial infarct size in STEMI patients undergoing primary PCI and provide evidence-based support for myocardial protection strategies of STEMI patients.

关键词： Cardiovascular disease Myocardial infarct size Nicorandil Primary percutaneous coronary intervention ST-segment elevation myocardial infarction

Genotype comparisons of strains of Candida albicans from patients with cutaneous candidiasis and vaginal candidiasis

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Chinese Medical Journal 2008年第15期121卷 1450-1455页

作者： SHE xiao-dong WANG Xue-jun FU Mei-hua SHEN Yong-nian LIU Wei-da Department of Medical Mycology Institute of Dermatology Chinese Academy of Medical Sciences & Peking Union Medical College Nanjing Jiangsu 210042 China Dalian Hospital of Dermatology Dalian Liaoning 116021 China

Background It is uncertain whether genotypes of Candida albicans （C. albicans） are associated with colonizing body locations or variant conditions of infection. The aim of this study was to investigate whether there are significant associations between strain genotypes and body sites of infection and to determine the potential pathogenesis of cutaneous candidiasis at multiple locations. Methods A total of 151 strains of C. albicans were isolated from 74 infant patients with cutaneous candidiasis and 61 female patients with vaginal candidiasis. Patients were grouped according to the body sites and underlying conditions of infection. Genotypes were identified by polymerase chain reaction （PCR） of the 25S rDNA and PCR-restriction fragment length polymorphism （RFLP） of ALT repeats digested with EcoRI and Clal. Results Ten genotypes were detected. There were significant differences in genotype frequencies between the two groups. However, we found no clear association between genotypes and the sites of cutaneous infection or the underlying conditions of vaginal candidiasis （VVC）. In addition, strains of C. albicans from multiple cutaneous locations of the same patient had identical genotypes. Conclusions Populations of C. albicans from patients with cutaneous and vaginal candidiasis were genetically different. However, the lack of genetic difference between strains from different body sites with cutaneous infections or from different underlying conditions for VVC suggests no evidence of genotype selection for different skin surfaces or patients with different underlying conditions for VVC.

关键词： cutaneous candidiasis vaginal candidiasis multiple locations genotype

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带结构风险最小化的最优区间回归模型辨识

控制理论与应用 2020年第3期37卷 560-573页

作者：刘小雍方华京陈孝玉遵义师范学院工学院贵州遵义563006 华中科技大学自动化学院湖北武汉430074

针对来自模型结构、参数以及测量数据的不确定性等因素,传统的辨识方法获取的是确定性数学模型的点输出,其鲁棒性差,易受外界干扰.因此,采用区间输出比点输出更易于实际问题的研究.基于复杂系统的不确定性测量数据以及系统参数的不确定... 详细信息

针对来自模型结构、参数以及测量数据的不确定性等因素,传统的辨识方法获取的是确定性数学模型的点输出,其鲁棒性差,易受外界干扰.因此,采用区间输出比点输出更易于实际问题的研究.基于复杂系统的不确定性测量数据以及系统参数的不确定性,提出了最优区间回归模型辨识的一种新方法,该方法将逼近误差的L∞范数思想与结构风险最小化理论相结合,建立求解区间模型的最优化问题,应用线性规划独立求解区间模型的上界和下界模型.该方法在保证模型辨识精度的同时,其泛化性能得到进一步提高.实验分析表明,提出的方法对来自噪声以及参数不确定性的数据,可以从区间模型的辨识精度和泛化性能之间取其平衡.

关键词：结构风险最小化不确定性分析逼近误差的L∞范数优化最优区间回归模型线性规划

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基于混合群集智能算法的并行公差优化设计

计算机集成制造系统 2007年第4期13卷 668-674,691页

作者：肖人彬陶振武邹洪富华中科技大学管理学院湖北武汉430074 华中科技大学CAD中心湖北武汉430074

通过对并行公差优化设计的分析,将其视为一种混合变量组合优化问题。首先给出了并行公差优化设计的数学模型,然后将其映射为一类特殊的旅行商问题——顺序多路旅行商问题,从而降低了问题的求解难度。利用蚁群优化算法和粒子群优化算法,... 详细信息

通过对并行公差优化设计的分析,将其视为一种混合变量组合优化问题。首先给出了并行公差优化设计的数学模型,然后将其映射为一类特殊的旅行商问题——顺序多路旅行商问题,从而降低了问题的求解难度。利用蚁群优化算法和粒子群优化算法,分别在求解离散和连续变量优化时的优势,提出了一种求解并行公差优化设计问题的混合群集智能算法。通过一个计算实例,将混合群集智能算法分别与遗传算法和模拟退火算法进行了比较,结果表明,前者具有更强的搜索能力和较高的效率。同时,混合群集智能算法也为求解一般意义的混合变量优化问题提供了借鉴和参考。

关键词：并行公差设计混合变量优化群集智能旅行商问题

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单模热腔场中两原子的纠缠演化动力学

北京理工大学学报 2009年第5期29卷 379-381,397页

作者：刘瑾邵彬向少华邹健北京理工大学理学院北京100081

在考虑内禀消相干影响下,通过使用部分转置的负本征值(NPT)和共生纠缠度来度量原子间的纠缠.研究了两个二能级原子与初态为热态的单模光场相互作用系统中,两原子间的纠缠随时间演化问题.讨论了内禀消相干和平均光子数等对纠缠度的影响.... 详细信息

在考虑内禀消相干影响下,通过使用部分转置的负本征值(NPT)和共生纠缠度来度量原子间的纠缠.研究了两个二能级原子与初态为热态的单模光场相互作用系统中,两原子间的纠缠随时间演化问题.讨论了内禀消相干和平均光子数等对纠缠度的影响.结果表明:存在内禀消相干时随着时间的演化,原子间的纠缠逐渐减小到一个确定值.通过对两种不同的纠缠度量方法的比较,可以看出NPT和共生纠缠度的时间演化有相似的规律,能够度量同类系统的纠缠.

关键词：纠缠内禀消相干部分转置的负本征值共生纠缠度

The expression and significance of CATSPER1 in human testis and ejaculated spermatozoa

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Asian Journal of Andrology 2006年第3期8卷 301-306,386页

作者： Hong-Gang Li Ai-hua Liao xiao-Fang Ding Hui Zhou Cheng-Liang Xiong Centre of Reproductive Medicine.Tongji Medical College Huazhong University of Science and TechnologyWuhan 430030China

Aim:To investigate the distribution of cation channel of sperm 1(CATSPER1)protein and the presence of CATSPER1 mRNA in human testis and ejaculated *** influence of anti-human CATSPER1 antibody upon human sperm motility was used to evaluate the function of human CATSPER1 and to estimate its possible use as a target for ***:Human ejaculated sperm from normozoospermic donors(n=12)and liquid nitro- gen frozen human testis were used for the study of mRNA and protein expression of CATSPER1 by reverse transcliption polymerase chain reaction(RT-PCR)and immunohistochemistry,*** from nonnozoospermic donors(n=12)were individually processed using a swim-up procedure and were then incubated with CATSPER 1 antibody at final concentrations of 20,4 and 0.8μg/*** 1,2 and 6h incubation,progressive motility and fast progressive motility were measured by means of computer-assisted semen ***:CATSPER1 transcript was detected in both human testis and each human ejaculated semen ***1 protein expressed in the membrane of spermatid and was localized in the principal piece of the sperm *** application of CATSPER1 antibody at all concentrations significantly inhibited both progressive motility and fast progressive motility after 1,2 and 6h incubation,and significant dose-dependent changes were ***:CATSPER1 is meiotically and post-meiotically expressed in human testis ***1 mRNA in human ejaculated spermatozoa could be a more feasible target for study and infertility screening than testis *** addition,our results suggest that human CATSPER1 could be a possible target for immunocontraception.(Asian J Androl 2006 May:8:301-306)

关键词： (?)ation channel of sperm 1 testis spermatozoa motility immunocontraception human

Adherence and invasion of mouse-adapted H pylori in different epithelial cell lines

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World Journal of Gastroenterology 2007年第6期13卷 845-850页

作者： Mao-Jun Zhang Fan-Liang Meng xiao-Yun Ji Li-hua He Jian-Zhong Zhang Department of Diagnostics National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Supported by the National Natural Science Foundation of China No. 30370078

AIM： To assess the adhesion and invasion abilities of different mouse adapted H py/or/strains in different cell lines in vitro and investigate their effects on the virulence factors cagA and vacA. METHODS： The adherence and invasion abilities of different N pylori strains in different epithelial cell lines were examined by the gentamycin protection assay. The null mutants of cagA and vacA were processed by direct PCR mutation method. The morphologic changes of different cell lines after N pylori attachment were examined by microscopy. RESULTS： The densities of adherence to and invasion into cells in vitro were different from those in the mouse infection experiments. 88-3887 strain could invade and adhere to cells stronger than SS1 and X47. All tested strains had better adhering and invasive abilities in SCG-7901 cell. CagA and vacA minus mutants had the same invasion and adherent abilities as their wild types. In all strains and cell lines tested, only AGS cell had the significant hummingbird phenotype after inoculation with the 88-3887 wild-type. CONCLUSION： Both the host cells and the bacteria play important parts in the invasion and adhesion abilities of Hpylori. CagA and VacA are not related to the ability of invasion and adhesion of Hpylori in different cell lines in vitro.

关键词： Hpylori Adherence Invasion Cell line