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Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

Molecular Plant 2024年第1期17卷 158-177页

作者： Peng Li Meng-xiao yan Pan Liu Dan-Jie yang Ze-Kun He Yun Gao yan Jiang Yu Kong Xin Zhong Sheng wu Jun yang Hong-Xia Wang yan-Bo Huang Le Wang xiao-Ya Chen Yong-Hong Hu Qing Zhao Ping Xu shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai Chenshan Plant Science Research CenterChineseAcademy of SciencesShanghaiChina College of Life Sciences Shanghai Normal UniversityShanghai 200234China State Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina University of the Chinese Academy of Sciences Beijing 100049China The Research Center of Chiral Drugs Innovation Research Institute of Traditional Chinese MedicineShanghai University of Traditional Chinese Medicine1200 Cailun RoadShanghai 201203China College of Chemistry and Chemical Engineering Shanghai University of Engineering ScienceShanghai 201620China

The Lamiaceae family is renowned for its terpenoid-based medicinal components,but Leonurus,which has traditional medicinal uses,stands out for its alkaloid-rich ***,the principal active compound found in Leonurus,has demonstrated promising effects in reducing blood lipids and treating ***,the biosynthetic pathway of leonurine remains largely ***,we present the chromosome-level genome sequence assemblies of Leonurus japonicus,known for its high leonurine production,and Leonurus sibiricus,characterized by very limited leonurine *** integrating genomics,RNA sequencing,metabolomics,and enzyme activity assay data,we constructed the leonurine biosynthesis pathway and identified the arginine decarboxylase(ADC),uridine diphosphate glucosyltransferase(UGT),and serine carboxypeptidase-like(SCPL)acyltransferase enzymes that catalyze key reactions in this *** analyses revealed that the UGT–SCPL gene cluster evolved by gene duplication in the ancestor of Leonurus and neofunctionalization of SCPL in ***,which contributed to the accumulation of leonurine specifically in ***,our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.

关键词： Lamiaceae Leonurus japonicus Leonurus sibiricus genome sequence alkaloids leonurine gene cluster biosynthetic pathway evolution

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Hydrothermal hydrogenation/deoxygenation of palmitic acid to alkanes over Ni/activated carbon catalyst

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Chinese Journal of Chemical Engineering 2024年第2期66卷 8-18页

作者： Min Lin Yuhao yan xiaoxian Li Rui Li Yulong wu MOE Engineering Center of Forestry Biomass Materials and Bioenergy Beijing Forestry UniversityBeijing 100083China Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing 100084China Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education Tsinghua UniversityBeijing 100084China

To produce paraffin from hydrogenation/deoxygenation of palmitic acid,model compound of bio-oil obtained by hydrothermal liquefaction(HTL)of microalgae has been an attractive focus in recent *** order to avoid energy-intensive separation process of water and bio-oil,it is of importance that deoxygenation upgrading of fatty acids under hydrothermal conditions similar to HTL ***,it is the first time to explore the application of activated carbon(AC)-supported non-noble-metal catalysts,such as Ni,Co,and Mo,and so on,in the hydrothermal hydrogenation/deoxygenation of long-chain fatty acids,and the obtained Ni/AC-H(the Ni/AC was further H_(2)pre-reduced)is one of the best *** addition,it is found that the catalytic activity can be further improved by H_(2)pre-reduction of *** results that are more low valences of nickel and oxygen vacancy can be obtained after H_(2)pre-reduction,thus significant promoting the deoxygenation especially the decarbonylation pathway of fatty *** total alkanes yield can reaches 95.9%at optimal conditions(280℃,360 min).This work confirmed that the low-priced AC-supported non-noble-metal catalysts have great potential compared with the noble-metal catalyst,in hydrothermal upgrading of bio-oil.

关键词： Bio-oil Fatty acids Deoxygenation Hydrothermal upgrading

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Two-person device-free localization system based on ZigBee and transformer

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High Technology Letters 2024年第1期30卷 61-67页

作者：刘天蒙 yanG Hai xiao wu Hong School of Electronic Information and Optical Engineering Nankai UniversityTianjin 300350P.R.China Tianjin Key Laboratory of Optoelectronic Sensor and Sensing Network Technology Nankai UniversityTianjin 300350P.R.China Engineering Research Center of Thin Film Optoelectronics Technology Nankai UniversityTianjin 300350P.R.China

Most studies on device-free localization currently focus on single-person *** paper proposes a novel method for device-free localization that utilizes ZigBee received signal strength indication(RSSI)and a Transformer network *** method aims to address the limited research and low accuracy of two-person device-free *** paper first describes the construction of the sensor network used for collecting ZigBee *** then examines the format and features of ZigBee data *** algorithm design of this paper is then *** box plot method is used to identify abnormal data points,and a neural network is used to establish the mapping model between ZigBee RSSI matrix and localization *** neural network includes a Transformer encoder layer as the encoder and a fully connected network as the *** proposed method's classification accuracy was experimentally tested in an online test stage,resulting in an accuracy rate of 98.79%.In conclusion,the proposed two-person localization system is novel and has demonstrated high accuracy.

关键词： device-free localization deep learning ZigBee

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A survey of energy-efficient strategies for federated learning in mobile edge computing

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Frontiers of Information Technology & Electronic Engineering 2024年第5期25卷 645-663页

作者： Kang yan Nina SHU Tao wu Chunsheng LIU Panlong yanG School of Electronic Engineering National University of Defense TechnologyHefei 230009China Department of Computing Hong Kong Polytechnic UniversityHong Kong 999077China School of Computer Science and Technology University of Science and Technology of ChinaHefei 230026China

With the booming development of fifth-generation network technology and Internet of Things,the number of end-user devices(EDs)and diverse applications is surging,resulting in massive data generated at the edge of *** process these data eficiently,the innovative mobile edge computing(MEC)framework has emerged to guarantee low latency and enable eficient computing close to the user ***,federated learning(FL)has demonstrated its empirical success in edge computing due to its privacy-preserving ***,it becomes a promising solution for analyzing and processing distributed data on EDs in various machine learning tasks,which are the major workloads in ***,EDs are typically powered by batteries with limited capacity,which brings challenges when performing energy-intensive FL *** address these challenges,many strategies have been proposed to save energy in *** the absence of a survey that thoroughly summarizes and classifies these strategies,in this paper,we provide a comprehensive survey of recent advances in energy-efficient strategies for FL in ***,we first introduce the system model and energy consumption models in FL,in terms of computation and *** we analyze the challenges regarding improving energy efficiency and summarize the energy-efficient strategies from three perspectives:learning-based,resource allocation,and client *** conduct a detailed analysis of these strategies,comparing their advantages and ***,we visually illustrate the impact of these strategies on the performance of FL by showcasing experimental ***,several potential future research directions for energy-efficient FL are discussed.

关键词： Mobile edge computing Federated learning Energy-efficient

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Synergic catalysis of W and Ni originating from substitution of trivacant phosphotungstate for the selective oxidation of aniline to azoxybenzene

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Chinese Chemical Letters 2024年第2期35卷 558-563页

作者： Sheng Cai xiao-Yuan wu Weiming wu Sa-Sa Wang Can-Zhong Lu CAS Key Laboratory of Design and Assembly of Functional Nanostructures and Fujian Provincial Key Laboratory of NanomaterialsFujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China College of Chemistry and Materials Science Fujian Normal UniversityFuzhou 350117China Xiamen Institute of Rare Earth Materials Chinese Academy of SciencesXiamen 361021China University of Chinese Academy of Sciences Beijing 100049China

Partial substitution of polyoxometalate(POM)is an efficient route to modulate the catalytic property of maternal *** this work,a new Keggin type POM involving{Ni 6}cluster,{[Ni(H_(2) O)_(2)(Dach)_(2)][Ni(Dach)_(2)]_(2)}{[Ni_(6)Cl(μ-OH)_(3)(H_(2) O)(Dach)_(3)(WO_(4))(PW9 O_(3)_(4))][Ni_(6)(μ-OH)_(3)(H_(2) O)_(2)(Dach)_(3)(WO_(4))(PW9 O_(3)_(4))]}Cl·27H_(2) O,(1,Dach=1,2-diaminocyclohexane)was *** 1 shows excellent catalytic performance in the selective oxidation of aniline to azoxybenzene(AOB)in *** apparently different results from that with the matrix{PW 9 O_(3)_(4)}({PW9})suggest the successful regulation of the catalytic property of{PW9}by the introduction of the{Ni6}cluster into the *** experimental results indicate that the highlighted performance of 1 is contributed by the synergy of W and Ni sites,which are respectively responsible for the oxidation and condensation steps in the production of *** good selectivity to AOB is essentially attributed to the effective modulation of the reaction rates of oxidation and condensation steps by W and Ni sites,respectively.

关键词： Polyoxometalate {Ni6}cluster Modulated catalytic property Aniline oxidation Azoxybenzene

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Pannexins in the musculoskeletal system:new targets for development and disease progression

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Bone Research 2024年第2期12卷 255-269页

作者： yan Luo Shengyuan Zheng Wenfeng xiao Hang Zhang Yusheng Li Department of Orthopedics Xiangya HospitalCentral South UniversityChangshaHunan 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Department of Clinical Medicine Xiangya Medicine SchoolCentral South UniversityChangshaHunan 410008China Shenzhen Institute of Advanced Technology Chinese Academy of SciencesShenzhen 518055China

During cell differentiation,growth,and development,cells can respond to extracellular stimuli through communication ***(Panx)family and connexin(Cx)family are two important types of channel-forming *** family contains three members(Panx1-3)and is expressed widely in bone,cartilage and *** there is no sequence homology between Panx family and Cx family,they exhibit similar configurations and *** to Cxs,the key roles of Panxs in the maintenance of physiological functions of the musculoskeletal system and disease progression were gradually revealed ***,we seek to elucidate the structure of Panxs and their roles in regulating processes such as osteogenesis,chondrogenesis,and muscle *** also focus on the comparison between Cx and *** a new key target,Panxs expression imbalance and dysfunction in muscle and the therapeutic potentials of Panxs in joint diseases are also discussed.

关键词： skeletal diseases system

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A Dielectric Ink Combining Acrylate and Cyanate Moieties for Inkjet 3D Printing with Good Thermal Stability

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Chinese Journal of Polymer Science 2024年第6期42卷 766-774页

作者： Yi-Xuan Li Liang-Ying wu yan yang Yao-Zhong Lu Zhong-Ying Ji Yu-Xiong Guo xiao-Long Wang Qiao-Sheng Pu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou730000China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsLanzhou730000China Lanzhou Traditional Chinese Medicine Hospital of Orthopedics and Traumatology Lanzhou730000China Shandong Laboratory of Advanced Materials and Green Manufacturing at yantai Yantai Zhongke Research Institute of Advanced Materials and Green Chemical EngineeringYantai264006China

Inkjet 3D printing has potential in the additive manufacturing of electronic circuits and ***,inks that can be used for printing layers with T5%>300℃ or hardness>200 MPa have been rarely *** ester(CE)polymers have excellent thermal stability,high strength,and low shrinkage compared to other common dielectric inks for inkjet 3D printing,but cannot be quickly shaped by ultraviolet(UV)irradiation or thermal *** CEs with UV-curable monomers may be a possible way to accelerate crosslinking,but there are challenges from the adverse effects of the dilution of both *** this study,dielectric inks with acrylate and cyanate moieties were *** low viscosity and surface tension of the CE precursor(Bisphenol E cyanate ester)were combined with photocurable acrylate diluent monomers and cross-linker to realize an ink suitable for inkjet 3D *** internal dual three-dimensional cross-linked network structure resin was prepared by a combination of photocuring and thermal curing with T5%up to 326.69℃,hardness up to 431.84 MPa,dielectric constant of 2.70 at 8 GHz,and shrinkage of 1.64%.The developed dielectric inks can be applied to the 3D printing of printed circuit boards and other electronic devices that require dielectric properties.

关键词： Inkjet printing Cyanate ester resin 3D doubly cross-linked network Good thermal stability

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Preferential adsorption control: Ca-based LDO for regenerating used lubricating oil

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Progress in Natural Science:Materials International 2024年第1期34卷 155-161页

作者： xiao-lei Zhang Jian-zhong wu Yun Liu Zai-wu Zhang Chao Hu Yong-sheng Lu School of Environmental and Chemical Engineering Shanghai University Shanghai Chengtou Wastewater Treatment CO. LTD Shanghai Shangsheng Ecological Engineering Co. Ltd. Shencai Technology Co. Ltd

Different Ca-based layered double oxides（LDO） were applied in the removal of contaminants in used lubricating oil as an alternative decolorant. CaAl-LDO and CaFeAl-LDO achieved much greater decoloration efficiency than the traditional decolorants, where 0.93 and 0.91 of the decoloration efficiency under the optimum conditions were obtained, respectively. The decoloration activity with CaFeAl-LDO > CaAl-LDO >> CaFe-LDO was revealed by both Density Functional Theory（DFT） calculations and experimental analysis. Ca12Al14O33was shown to be the active component for decoloration. Fe3+doped into CaAl-LDO could improve decoloration activity via a changed charge distribution of decolorant. High preferential adsorption activity, which originated from different charge distribution for contaminants instead of the oil components, contributed to outstanding decoloration performance. The activity depended on the adsorption intensity of contaminants, where the most of the active decolorant neither too strongly or weakly bound with the contaminants. The results of this study suggested the Cabased LDOs could be a potentially alternative decolorants for regenerating the used lubricating oil.

关键词： Used lubrication oil Adsorption Ca-LDO Decoloration DFT calculation

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RNF187 governs the maintenance of mouse GC-2 cell development by facilitating histone H3 ubiquitination at K57/80

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Asian Journal of Andrology 2024年第3期26卷 272-281页

作者： Bing-Ya Xu Xiang-Ling Yu Wen-Xin Gao Ting-Ting Gao Hao-Yue Hu Tian-Tian wu Cong Shen xiao-yan Huang Bo Zheng Yi-Bo wu Human Reproductive and Genetic Center Affiliated Hospital of Jiangnan UniversityWuxi 214122China State Key Laboratory of Reproductive Medicine Department of Histology and EmbryologySchool of Basic Medical SciencesNanjing Medical UniversityNanjing 211166China Changzhou Maternal and Child Health Care Hospital Changzhou Medical CenterNanjing Medical UniversityChangzhou 213000China State Key Laboratory of Reproductive Medicine Center for Reproduction and GeneticsSuzhou Municipal HospitalThe Affiliated Suzhou Hospital of Nanjing Medical UniversityGusu SchoolNanjing Medical UniversitySuzhou 215002China

RING finger 187(RNF187),a ubiquitin-ligating(E3)enzyme,plays a crucial role in the proliferation of cancer ***,it remains unclear whether RNF187 exhibits comparable functionality in the development of germline *** investigate thepotential involvement of RNF187 in germ cell development,we conducted interference and overexpression assays using GC-2 cells,a mouse spermatocyte-derived cell *** findings reveal that the interaction between RNF187 and histone H3 increases theviability,proliferation,and migratory capacity of GC-2 ***,we provide evidence demonstrating that RNF187 interactswith H3 and mediates the ubiquitination of H3 at lysine 57(K57)or lysine 80(K80),directly or indirectly resulting in increasedcellular *** is a study to report the role of RNF187 in maintaining the development of GC-2 cells by mediatinghistone H3 ubiquitination,thus highlighting the involvement of the K57 and K80 residues of H3 in the epistatic regulation of *** discoveries provide a new theoretical foundation for further comprehensive investigations into the functionof RNF187 in the reproductive system.

关键词： GC-2 cell histone H3 RNF187 ubiquitination

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Impact of transcranial electrical stimulation on serum neurotrophic factors and language function in patients with speech disorders

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World Journal of Clinical Cases 2024年第10期12卷 1742-1749页

作者： Li Sun Kai xiao xiao-yan Shen Shu Wang Department of Rehabilitation Medicine General Hospital of the Yangtze River ShippingWuhan 430010Hubei ProvinceChina

BACKGROUND Speech disorders have a substantial impact on communication abilities and quality of *** treatments such as speech and psychological therapies frequently demonstrate limited effectiveness and patient *** electrical stimulation(TES)has emerged as a promising non-invasive treatment to improve neurological ***,its effectiveness in enhancing language functions and serum neurofactor levels in individuals with speech disorders requires further *** To investigate the impact of TES in conjunction with standard therapies on serum neurotrophic factor levels and language function in patients with speech *** In a controlled study spanning from March 2019 to November 2021,81 patients with speech disorders were divided into a control group(n=40)receiving standard speech stimulation and psychological intervention,and an observation group(n=41)receiving additional *** study assessed serum levels of ciliary neurotrophic factor(CNTF),glial cell-derived neurotrophic factor(GDNF),brainderived neurotrophic factor(BDNF),and nerve growth factor(NGF),as well as evaluations of motor function,language function,and development quotient *** After 3 wk of intervention,the observation group exhibited significantly higher serum levels of CNTF,GDNF,BDNF,and NGF compared to the control ***,improvements were noted in motor function,cognitive function,language skills,physical abilities,and overall development quotient *** is worth mentioning that the observation group also displayed superior perfor CONCLUSION This retrospective study concluded that TES combined with traditional speech and psychotherapy can effectively increase the levels of neurokines in the blood and enhance language function in patients with speech *** results provide a promising avenue for integrating TES into standard treatment methods for speech disorders.

关键词： Transcranial electrical stimulation Serum neurofactor levels Developmental level Language features

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