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Sequential expression of miR-221-3p and miR-338-3p in Schwann cells as a therapeutic strategy to promote nerve regeneration and functional recovery

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Neural Regeneration Research 2023年第3期18卷 671-682页

作者： Li-Li Wen Tian-Hao Yu Yi-Zhan Ma xiao-yan mao Tian-Rang Ao Rabia Javed Hirotomo Ten Akira Matsuno Qiang Ao Department of Tissue Engineering School of Intelligent MedicineChina Medical UniversityShenyangLiaoning ProvinceChina The VIP Department School and Hospital of StomatologyLiaoning Provincial Key Laboratory of Oral DiseasesChina Medical UniversityShenyangLiaoning ProvinceChina Department of Oncology Cancer HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijingChina Department of Neurosurgery Teikyo University School of MedicineTokyoJapan NMPA Key Laboratory for Quality Research and Control of Tissue Regenerative Biomaterial&Institute of Regulatory Science for Medical Device&National Engineering Research Center for Biomaterials Sichuan UniversityChengduSichuan ProvinceChina

The functional properties of endogenous Schwann cells(SCs)during nerve repair are *** the functional properties of SCs at different stages of nerve repair may have therapeutic benefit in improving the repair of damaged *** studies showed that miR-221-3p promotes the proliferation and migration of SCs,and miR-338-3p promotes the myelination of *** this study,we established rat models of sciatic nerve injury by bridging the transected sciatic nerve with a silicone *** injected a miR-221 lentiviral vector system together with a doxycycline-inducible Tet-On miR-338 lentiviral vector system into the cavity of nerve conduits of nerve stumps to sequentially regulate the biological function of endogenous SCs at different stages of nerve *** found that the biological function of SCs was sequentially regulated,the diameter and density of myelinated axons were increased,the expression levels of NF200 and myelin basic protein were increased,and the function of injured peripheral nerve was improved using this *** Target Prediction Database prediction,Nanopore whole transcriptome sequencing,quantitative PCR,and dual luciferase reporter gene assay results predicted and verified Cdkn1b and Nrp1 as target genes of miR-221-3p and miR-338-3p,respectively,and their regulatory effects on SCs were confirmed in *** conclusion,here we established a new method to enhance nerve regeneration through sequential regulation of biological functions of endogenous SCs,which establishes a new concept and model for the treatment of peripheral nerve *** findings from this study will provide direct guiding significance for clinical treatment of sciatic nerve injury.

关键词： cdkn1b miR-221 miR-338 miRNA nerve regeneration Nrp1 peripheral nerve injury regulation Schwann cells sequential expression

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Motivations and Training Needs of General Practitioner Preceptors

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Chinese Medical Journal 2017年第14期130卷 1689-1693页

作者： Wen Ren Nulanbieke Hasenbieke Ying Liu yan Qiu Zhao-Nong Zhou xiao-yan mao Jing-Jing Ren Department of General Practice The First Affiliated Hospital College of Medicine Zhejiang University Hangzhou Zhejiang 310003 China Training Center of the National Population and Family Planning Commission Beijing 100024 China Department of Comprehensive Training School of Continuing Education Zhejiang University Hangzhou Zhejiang 310003 China

Background：General practitioner （GP） preceptors play an important role in the cultivation of *** problems exist in the training of GP *** study aimed to explore the willingness and training needs of GP preceptors and compare the differences between preceptors from general practice and other ***：A total of 375 questionnaire forms were sent to 375 GP preceptors from 11 different provinces,and 344 completed forms were *** main outcome included general information,teaching motivations,and training needs of GP ***：The study showed that about 89.2% of GP preceptors were willing to be *** majority of respondents strongly agreed that the motivation for becoming a GP supervisor was to learn from *** most important capability they should master was clinical teaching （92.2%）,followed by lecture （83.1%） and doctor-patient communication （83.1%）.The top three preferred methods of GP preceptors training were case discussion （78.8%）,workshop （57.6%）,and classroom teaching （56.4%）.The domains in which most GP preceptors wanted to acquire knowledge and skill were mental health （59.3%）,rehabilitation （47.1%）,pediatrics （41.0%）,and obstetrics （37.5%）.No significant differences were found in the willingness to train GPs （x2 =3.34,P 〉 0.05） and whether they would become or continue to become a GP supervisor after the training （x2 =l.106,P 〉 0.05）.Conclusions：Although most preceptors were under on-the-job training,they were glad to train *** be qualified,preceptors should be trained according to the actual needs of GP preceptors.

关键词： General Practitioner Health Educator: Motivation: Needs Assessment

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Immunogenicity and safety of NVSI-06-07 as a heterologous booster after priming with BBIBP-CorV:a phase 2 trial

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Signal Transduction and Targeted Therapy 2022年第7期7卷 2522-2532页

作者： Nawal Al Kaabi Yun Kai yang Jing Zhang Ke Xu Yu Liang Yun Kang Ji Guo Su Tian yang Salah Hussein Mohamed Saif ElDein Shuai Shao Sen Sen yang Wenwen Lei Xue Jun Gao Zhiwei Jiang Hui Wang Meng Li Hanadi Mekki Mekki Walid Zaher Sally Mahmoud Xue Zhang Chang Qu Dan Ying Liu Jing Zhang Mengjie yang Islam Eltantawy Peng xiao Zhao Nian Wang Jin Liang Yin xiao yan mao Jin Zhang Ning Liu Fu Jie Shen Liang Qu Yun Tao Zhang xiao Ming yang Guizhen Wu Qi Ming Li Sheikh Khalifa Medical City SEHAAbu DhabiUnited Arab Emirates College of Medicine and Health Sciences Khalifa UniversityAbu DhabiUnited Arab Emirates China National Biotec Group Company Limited BeijingChina The Sixth Laboratory National Vaccine and Serum Institute(NVSI)BeijingChina National Engineering Center for New Vaccine Research BeijingChina National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention(China CDC)BeijingChina Lanzhou Institute of Biological Products Company Limited LanzhouChina Beijing Key Tech Statistical Consulting Co. LtdBeijingChina Beijing Institute of Biological Products Company Limited BeijingChina Union 71 Abu DhabiUnited Arab Emirates G42 Healthcare Abu DhabiUnited Arab Emirates

The increased coronavirus disease 2019(COVID-19)breakthrough cases pose the need of booster *** conducted a randomised,double-blinded,controlled,phase 2 trial to assess the immunogenicity and safety of the heterologous prime-boost vaccination with an inactivated COVID-19 vaccine(BBIBP-CorV)followed by a recombinant protein-based vaccine(NVSI-06-07),using homologous boost with BBIBP-CorV as *** groups of healthy adults(600 individuals per group)who had completed two-dose BBIBP-CorV vaccinations 1–3 months,4–6 months and≥6 months earlier,respectively,were randomly assigned in a 1:1 ratio to receive either NVSI-06-07 or BBIBP-CorV *** assays showed that in NVSI-06-07 groups,neutralizing antibody geometric mean titers(GMTs)against the prototype SARS-CoV-2 increased by 21.01–63.85 folds on day 28 after vaccination,whereas only 4.20–16.78 folds of increases were observed in control *** Omicron variant,the neutralizing antibody GMT elicited by homologous boost was 37.91 on day 14,however,a significantly higher neutralizing GMT of 292.53 was induced by heterologous *** results were obtained for other SARS-CoV-2 variants of concerns(VOCs),including Alpha,Beta and *** heterologous and homologous boosters have a good safety *** and systemic adverse reactions were absent,mild or moderate in most participants,and the overall safety was quite similar between two booster *** findings indicated that NVSI-06-07 is safe and immunogenic as a heterologous booster in BBIBP-CorV recipients and was immunogenically superior to the homologous booster against not only SARS-CoV-2 prototype strain but also VOCs,including Omicron.

关键词： phase neutral GMT

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综合面向对象与决策树的毛竹林调查因子及碳储量遥感估算

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应用生态学报 2017年第10期28卷 3163-3173页

作者：杜华强孙晓艳韩凝毛方杰省部共建亚热带森林培育国家重点实验室浙江临安311300 浙江省森林生态系统碳循环与固碳减排重点实验室浙江临安311300 浙江农林大学环境与资源学院浙江临安311300

综合面向对象和CART决策树方法,对浙江省安吉县山川乡毛竹林分布信息及胸径、树高、郁闭度等调查因子和地上部分碳储量进行遥感定量估算.结果表明:综合基于多尺度分割的对象特征及决策树,能够充分利用不同尺度层次信息关联的优势,实现... 详细信息

综合面向对象和CART决策树方法,对浙江省安吉县山川乡毛竹林分布信息及胸径、树高、郁闭度等调查因子和地上部分碳储量进行遥感定量估算.结果表明:综合基于多尺度分割的对象特征及决策树,能够充分利用不同尺度层次信息关联的优势,实现毛竹林专题信息高精度提取,其用户精度达到89.1%;基于对象特征构建的毛竹林调查因子回归树估算模型,其估算结果均能达到正常或较好水平,其中,郁闭度回归树模型的精度最高为67.9%,估算效果较好;而平均胸径和树高估算的总精度相对较低,这与采用光学遥感数据进行森林树高、胸径估算达不到理想结果的结论一致;毛竹林地上部分碳储量回归树模型的估算结果较好,高值区域估算精度达到80%以上.

关键词：面向对象决策树森林调查参数地上碳储量 SPOT5

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High Fe‑Loading Single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

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Nano-Micro Letters 2025年第2期17卷 187-203页

作者： Si Chen Fang Huang Lijie mao Zhimin Zhang Han Lin Qixin yan Xiangyu Lu Jianlin Shi Department of Cardiology Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200092People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China Shanghai Frontiers Science Center of Nanocatalytic Medicine School of MedicineTongji UniversityShanghai 200092People’s Republic of China

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site ***,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with *** constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial ***,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading ***,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/*** addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)*** in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound *** work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

关键词： Nanocatalytic medicine Single-atom catalysts Reactive oxygen species(ROS) High metal loading Oxidase catalysis

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An investigation of acarbose effects in PCOS women with postprandial hyperglycemia

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生殖医学杂志 2002年第Z1期11卷 22-28页

作者： Zheng Jianhuai(郑建淮) Cao Zuansun(曹缵孙) Chen xiaoyan (陈晓燕) mao Wenjun(毛文军) Research Laboratory of Reproductive Endocrinology First Hospital of Xi''an Jiaotong University Xi''an 710061 Research Laboratory of Reproductive Endocrinology First Hospital of Xi''an Jiaotong University Xi''an 710061 Research Laboratory of Reproductive Endocrinology First Hospital of Xi''an Jiaotong University Xi''an 710061 Research Laboratory of Reproductive Endocrinology First Hospital of Xi''an Jiaotong University Xi''an 710061

Objectives: To investigate the effects of insulin resistance on serum androgen level and ovulation of women with polycystic ovary syndrome (PCOS) and observe clinic role of acarbose in the treatment of hyperinsulinemia, postprandial hyperglycemia and anovulation. Methods: 14 women accompanied by postprandial hyperglycemia with PCOS were administrated by acarbose for 12 weeks.14 age-matched individuals who had similar body mass index and normal menstruation were served as controls. Results: Serum T levels declined significantly from 4.09±1.04 nmol/L to 1.71±0.54 nmol/L (P<0.001), after acarbose treatment for 12 weeks. 12 out of 14 cases restored ovulation and menstrual cycles after acarbose treatment, among which 4 got pregnant. Conclusion: Acarbose may play a role on reducing postprandial hyperglycemia and HbAic levels, increase ISI and FSG/FI, indirectly reduce serum androgen levels through reducing plasma insulin level and recover ovarian ovulation in PCOS women with postprandial hyperglycemia.

关键词： Polycystic ovary syndrome Hyperinsulinemia Postprandialhyperglycemia Acarbose

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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu xiao-Jia yang Qian-Ru He Fei Ding Hai-yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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高校图书馆中文纸质学术期刊使用效益的调研和思考——以南京审计大学图书馆为例

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晋图学刊 2016年第6期 47-51,68页

作者：范小燕茆凌南京审计大学图书馆江苏南京211185

以南京审计大学期刊阅览室中文纸质学术期刊为数据源,采用中文纸质学术期刊阅读推广调查问卷,运用文献计量的方法,从中文学术期刊阅读方式选择、中文纸质学术期刊关注信息类型频率和选择中文纸质期刊阅读的因素频率三个方面解析中文纸... 详细信息

以南京审计大学期刊阅览室中文纸质学术期刊为数据源,采用中文纸质学术期刊阅读推广调查问卷,运用文献计量的方法,从中文学术期刊阅读方式选择、中文纸质学术期刊关注信息类型频率和选择中文纸质期刊阅读的因素频率三个方面解析中文纸质学术期刊使用效益低下的成因,从而找到提高其使用效益的路径。

关键词：中文纸质学术期刊使用效益电子学术期刊微信导读

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Metal nanoparticles decorated CoFe-(oxy)hydroxysulfides nanosheets fabricated by a general strategy for electrocatalytic water splitting

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Journal of Energy Chemistry 2025年第1期100卷 26-38页

作者： xiaodong yang Haochen Shen xiaoming xiao Zhichao Li Qi Zhou Wei yang Bin Jiang Yongli Sun Luhong Zhang Zhenhua yan School of Chemical Engineering and Technology Tianjin University Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education) Nankai University

This study presents a novel method to fabricate metal-decorated,sulfur-doped layered double hydroxides （M/SLDH） through spontaneous redox and sulfurization *** developed Ag/SLDH and Pt/SLDH catalysts with abundant heterogeneous interfaces and hierarchical nanostructures demonstrated outstanding oxygen evolution reaction （OER） and hydrogen evolution reaction （HER） performance,achieving low overpotentials of 212 and 35 mV at 10 mA cm-2in 1 M KOH,*** both anode and cathode in water splitting,they required only 1.47 V to reach 10 mA cm-2and exhibited high structural robustness,maintaining stability at 1000 mA cm-2for 300 ***-situ Raman analysis revealed that the synergistic effects of metal nanoparticles and S doping significantly promote the transformation into the S-Co1-xFexOOH layer,which serves as the active phase for water ***,ultraviolet photoelectron spectroscopy （UPS） and density functional theory （DFT） analyses indicated that incorporating metal nanoparticles and S doping increase electron density near the Fermi level and reduce reaction energy barriers,thus enhancing intrinsic OER and HER *** study provides a scalable strategy for synthesizing high-performance electrocatalysts for water splitting,with promising potential for broader applications.

关键词： LDH Spontaneous reaction Metal nanoparticles Water splitting

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Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang yanlin Fu Peiyan Long xiao Gao Zhengwei Wang Zhizhong Guan xiaolan Qi Wei Hong yan xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

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