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Anti-nociceptive Effect of Patchouli Alcohol: Involving Attenuation of Cyclooxygenase 2 and Modulation of Mu-Opioid Receptor

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Chinese Journal of Integrative Medicine 2019年第6期25卷 454-461页

作者： YU Xuan WANG Xin-pei yan xiao-jin JIANG jing-fei LEI Fan XING Dong-ming GUO Yue-ying DU Li-jun MOE Key Laboratory of Protein Sciences Laboratory of Molecular Pharmacology and Pharmaceutical Sciences School of Life Sciences Tsinghua University Beijing (100084) China School of Pharmacology and Pharmaceutical Sciences Tsinghua University Beijing (100084) China

Objective: To explore the anti-nociceptive effect of patchouli alcohol(PA), the essential oil isolated from Pogostemon cablin(Blanco) Bent, and determine the mechanism in molecular levels. Methods: The acetic acid-induced writhing test and formalin-induced plantar injection test in mice were employed to con?rm the effect in vivo. Intracellular calcium ion was imaged to verify PA on mu-opioid receptor(MOR). Cyclooxygenase 2(COX2)and MOR of mouse brain were expressed for determination of PA’s target. Cellular experiments were carried out to find out COX2 and MOR expression induced by PA. Results: PA significantly reduced latency period of visceral pain and writhing induced by acetic acid saline solution(P<0.01) and allodynia after intra-plantar formalin(P<0.01) in mice. PA also up-regulated COX2 mRNA and protein(P<0.05) with a down-regulation of MOR(P<0.05) both in in vivo and in vitro experiments, which devote to the analgesic effect of PA. A decrease in the intracellular calcium level(P<0.05) induced by PA may play an important role in its anti-nociceptive *** showed the characters of enhancing the MOR expression and reducing the intracellular calcium ion similar to opioid effect. Conclusions: Both COX2 and MOR are involved in the mechanism of PA’s anti-nociceptive effect,and the up-regulation of the receptor expression and the inhibition of intracellular calcium are a new perspective to PA’s effect on MOR.

关键词： Chinese medici ne patchouli alcohol an algesic effect mu-opioid receptor cyclooxyge nase 2

Brazilein inhibits neuronal inflammation induced by cerebral ischemia and oxygen-glucose deprivation through targeting NOD2 expression

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Chinese Journal of Natural Medicines 2016年第5期14卷 354-362页

作者： yan xiao-jin CHAI Yu-Shuang YUAN Zhi-Yi WANG Xin-Pei JIANG jing-Fei LEI Fan XING Dong-Ming DU Li-Jun MOE Key Laboratory of Protein Sciences Laboratory of Molecular Pharmacology and Pharmaceutical Sciences School of Life Sciences and School of Medicine Tsinghua University

Brazilein is reported to have immunosuppressive effect on cardiovascular and cerebral-vascular diseases. The essential roles of innate immunity in cerebral ischemia are increasingly identified, but no studies concerning the influence of brazilein on the innate immunity receptors have been reported. The present study was designed to investigate the regulation of NOD2 （Nucleotide-binding oligomerization domain-containing protein 2） by brazilein for its protection of neuron in cerebral ischemia in vivo and oxygen-glucose deprivation in vitro. The results showed that brazilein could reverse the elevated expression of NOD2 and TNFa （tumor necrosis factor alpha） elicited by cerebral ischemia and reperfusion. This reduction could also be detected in normal mice and C 17.2 cells, indicating that this suppressive effect of brazilein was correlated with NOD2. The results from GFP reporter plasmid assay suggested brazilein inhibited NOD2 gene transcription. In conclusion, brazilein could attenuate NOD2 and TNFα expression in cerebral ischemia and NOD2 may be one possible target of brazilein for its immune suppressive effect in neuro-inflammation.

关键词： Brazilein Neuroinflammation NOD2

Effects of Angelica dahurica on obesity and fatty liver in mice

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Chinese Journal of Natural Medicines 2016年第9期14卷 641-652页

作者： LU Xi YUAN Zhi-Yi yan xiao-jin LEI Fan JIANG jing-Fei YU Xuan yanG Xiu-Wei XING Dong-Ming DU Li-Jun MOE Key Laboratory of Protein Sciences Laboratory of Molecular Pharmacology and Pharmaceutical Sciences School of Life Sciences Tsinghua University Department of Pharmacology and Pharmaceutical Sciences School of Medicine Tsinghua University School of Pharmaceutical Sciences Beijing University

Angelica dahurica(A. dahurica) is a traditional Chinese medicinal plant being used in clinical practice. The present study demonstrated that A. dahurica could reduce white-fat weight in high-fat-diet hyperlipidemic mice, decrease total cholesterol and triglyceride concentrations in the livers of both high-fat-diet and Triton WR1339 induced hyperlipidemic mice, and enhance the total hepatic lipase activities of them. These findings were further supported by the results derived from the experiments with Hep G2 cells in vitro. In addition, the proteins related to lipids metabolism were investigated using LC-MS/MS, indicating that genes of lipid metabolism and lipid transport were regulated by A. dhurica. The results from LC-MS/MS were further conformed by Western blot and real time PCR assays. A. dahurica could down-regulate the expression of catalase(CAT) and sterol carrier protein2(SCP2) and up-regulate the expression of lipid metabolism related genes-lipase member C(LIPC) and peroxisome proliferator-activated receptor gamma(PPARγ). In the Triton WR1339 mouse liver and Hep G2 cells in vitro, A. dahurica was able to increase the expression of LIPC and PPARγ, confirming the results from in vivo experiments. Imperatorin showed the same activity as A. dahurica, suggesting it was one of the major active ingredients of the herb. In conclusion, our work represented a first investigation demonstrating that A. dahurica was able to regulate lipid metabolism and could be developed as a novel approach to fighting against fatty liver and obesity.

关键词： Angelica dahurica Imperatorin Hepatic lipases Mouse

Mechanism underlying berberine's effects on HSP70/TNFα under heat stress:Correlation with the TATA boxes

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Chinese Journal of Natural Medicines 2017年第3期15卷 178-191页

作者： JIANG jing-Fei LEI Fan YUAN Zhi-Yi WANG Yu-Gang WANG Xin-Pei yan xiao-jin YU Xuan XING Dong-Ming DU Li-Jun MOE(Ministry of Education)Key Laboratory of Protein Sciences Laboratory of Molecular Pharmacology and Pharmaceutical SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Department of Pharmacology and Pharmaceutical Sciences School of MedicineTsinghua UniversityBeijing 100084China MD Anderson Cancer Center University of TexasHoustonTX 77030USA

Heat stress can stimulate an increase in body temperature, which is correlated with increased expression of heat shock protein 70 （HSP70） and tumor necrosis factor a （TNFa）. The exact mechanism underlying the HSP70 and TNFa induction is unclear. Berberine （BBR） can significantly inhibit the temperature rise caused by heat stress, but the mechanism responsible for the BBR effect on HSP70 and TNFa signaling has not been investigated. The aim of the present study was to explore the relationship between the expression of HSP70 and TNFa and the effects of BBR under heat conditions, using in vivo and in vitro models. The expression levels of HSP70 and YNFa were determined using RT-PCR and Western blotting analyses. The results showed that the levels of HSP70 and TNFa were ap-regulated under heat conditions （40 ~C）. HSP70 acted as a chaperone to maintain TNFa homeostasis with rising the temperature, but knockdown of HSP70 could not down-regulate the level of TNFa. Furthermore, TNFa could not influence the expression of HSP70 under aormal and heat conditions. BBR targeted both HSP70 and TNFa by suppressing their gene transcription, thereby decreasing body temperature under heat conditions. In conclusion, BBR has a potential to be developed as a therapeutic strategy for suppressing the thermal effects in hot environments.

关键词： Berberine Hyperthermia HSP70 TNFa TATA box

Advances in endocrine and targeted therapy for hormone-receptorpositive,human epidermal growth factor receptor 2-negative advanced breast cancer

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Chinese Medical Journal 2020年第9期133卷 1099-1108页

作者： Le-Sang Shen xiao-yan jin Xu-Meng Wang Lai-Zhen Tou Jian Huang Department of Breast Surgery The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang 310009China Key Laboratory of Tumor Microenvironment and Immune Therapy of Zhejiang Province HangzhouZhejiang 310009China Department of Surgical Oncology Zhejiang Taizhou Municipal HospitalTaizhouZhejiang 318000China Department of Surgical Oncology the First Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang 310003China

Nearly 70%of breast cancer(BC)is hormone-receptor(HR)-positive,human epidermal growth factor receptor 2(HER2)-negative,and endocrine therapy is the mainstay of treatment for this ***,intrinsic or acquired endocrine resistance can occur during the endocrine *** on insights of endocrine resistance mechanisms,a number of targeted therapies have been and continue to be *** regard to HR-positive,HER2-negative advanced BC,aromatase inhibitor(AI)is superior to tamoxifen,and fulvestrant is a better option for patients previously exposed to endocrine *** drugs,such as cyclindependent kinases(CDK)4/6 inhibitors,mammalian target of rapamycin(mTOR)inhibitors,phosphoinositide-3-kinase(PI3K)inhibitors,and histone deacetylase(HDAC)inhibitors,play a significant role in the present and show a promising *** the application of CDK4/6 inhibitors becoming common,mechanisms of acquired resistance to them should also be taken into consideration.

关键词： Endocrine therapy Advanced breast cancer Endocrine resistance Targeted therapy

NONLINEAR OPTICAL STUDY OF Fe_2O_3 UFP SOL

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Chinese Chemical Letters 1991年第1期2卷 85-86页

作者： Bing Suo ZOHU Liang Zhi xiao yan ZHANG Tie jin LI Department of Chemistry.Jilin University.Changchun,130023Jia Long ZHAO Li HAN Hao Sheg FEI Department of Physics,Jilin University,Changchun,130023

In this letter,Fe_2O_3 UFP(Ultrafine Particles)hydrosol and organosol with high third order optical nonlinear susceptibility(3)have been reported for the first time. The surfacial modification of Fe_2O_3 UFP with dodecylbenzenesulfonate ions(DBS)can enhance the nonlinear optical response through interfacial confinement of electron motion. The involved mechanism is discussed.

关键词： Fe UFP NONLINEAR OPTICAL STUDY OF Fe2O3 UFP SOL DBS

2010—2014年江苏省灌云县疑似预防接种异常反应监测分析

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职业与健康 2016年第19期32卷 2675-2678页

作者：颜晓今灌云县疾病预防控制中心江苏连云港222200

目的分析灌云县2010—2014年疑似预防接种异常反应(AEFI)的发生特征,评价预防接种的安全性和AEFI监测系统运转情况。方法通过AEFI信息管理系统,收集灌云县2010—2014年报告的AEFI个案数据,采用描述流行病学方法进行分析。结果 2010—201... 详细信息

目的分析灌云县2010—2014年疑似预防接种异常反应(AEFI)的发生特征,评价预防接种的安全性和AEFI监测系统运转情况。方法通过AEFI信息管理系统,收集灌云县2010—2014年报告的AEFI个案数据,采用描述流行病学方法进行分析。结果 2010—2014年灌云县共报告AEFI 549例,报告发生率为34.47/10万剂。报告数居前3位的的疫苗分别是百白破(无细胞)234例(42.62%),发生率为95.67/10万剂;白破47例(8.56%),发生率为144.54/10万剂;b型流感嗜血杆菌(Hib)疫苗34例(6.19%),发生率为39.64/10万剂。男女性别比为1.42∶1;病例主要发生在≤2岁组人群,占65.03%;4—8月份报告病例最多,占59.93%。一般反应503例(皆为发热/红肿/硬结),占91.62%;异常反应46例,占8.38%,其中以过敏性皮疹最多,占异常反应的73.91%。75.23%的AEFI在接种后1 d发生;48 h内报告率及调查率为100%,未出现接种差错和疫苗质量事故。结论灌云县AEFI监测系统运转正常,但监测系统的敏感性仍需提高。

关键词：预防接种异常反应(AEFI) 报告发生率监测

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高校资料室工作与公关意识

长沙铁道学院学报（社会科学版） 2002年第1期3卷 125-126页

作者：晏小金中南大学铁道校区经济管理学院湖南长沙410075

运用公共关系学的基本理论,论述高校资料室工作引入公关概念,塑造良好形象,强化服务意识的重要性,从而加强资料室与教学、科研的勾通,以取得公众支持,充分发挥资料室的作用。

关键词：资料室公关意识塑造形象服务公众传播沟通

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灌云县正常人群甲肝免疫水平监测分析

中国校医 2015年第8期29卷 597-598页

作者：颜晓今灌云县疾病预防控制中心江苏灌云222200

目的掌握灌云县正常人群甲肝抗体水平,评价预防接种工作质量,为进一步制订甲肝防制策略提供科学依据。方法采用酶联免疫(ELISA)法对7个不同年龄组正常人群进行甲肝血清学Ig G抗体监测,并对结果进行几何平均滴度(GMT)、χ2检验和方差分... 详细信息

目的掌握灌云县正常人群甲肝抗体水平,评价预防接种工作质量,为进一步制订甲肝防制策略提供科学依据。方法采用酶联免疫(ELISA)法对7个不同年龄组正常人群进行甲肝血清学Ig G抗体监测,并对结果进行几何平均滴度(GMT)、χ2检验和方差分析。结果本次共检测350份血清标本,抗体阳性率为91.14%(319/350),GMT为1∶10.57。0岁组和1.5岁组抗体阳性率和GMT均较高,10岁组和20岁组则较低。结论加强甲肝疫苗基础免疫,适时对中小学生和大年龄组人群进行强化免疫,是今后控制甲肝的关键。

关键词：肝炎,甲型/免疫学抗体,病毒/血液