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Effects and mechanism of adenovirus-mediated phosphatase and tension homologue deleted on chromosome ten gene on collagen deposition in rat liver fibrosis

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World Journal of Gastroenterology 2017年第32期23卷 5904-5912页

作者： Shu-rui Xie Jun-yan An Li-bo Zheng xiao-xia huo Jian Guo David Shih xiao-Lan Zhang Department of Gastroenterology The Second Hospital of Hebei Medical University Hebei Key Laboratory of Gastroenterology Hebei Institute of Gastro- enterology Department of Gastroenterology Xingtai People's Hospital Inflammatory Bowel and Immunobiology Research Institute F. Widjaja Foundation Cedars-Sinai Medical Center

AIM To evaluate the effects of phosphatase and tension homologue deleted on chromosome ten(PTEN) gene on collagen metabolism in hepatic fibrosis and the underlying *** rat primary hepatic stellate cells(HSCs) and human LX-2 cells were transfected with adenovirus containing c DNA constructs encoding wild-type PTEN(Ad-PTEN), PTEN mutant G129 E gene(Ad-G129E), and r NA interference constructs targeting the PTEN sequence PTEN short hairpin r NA to up-regulate and downregulate the expression of PTEN. HSCs were assayed using fluorescent microscopy, real-time polymerase chain reaction, and western blotting. Moreover, a CCl_4-induced rat hepatic fibrosis model was established to investigate the in vivo effects. Hematoxylin and eosin, and Masson's trichrome were used to assess the histological changes. The expression of collagen Ⅰ and Ⅲ was assessed using immunohistochemistry and western blot *** Elevated expression of PTEN gene reduced serum levels of alanine transaminase and aspartate transaminase, decreased collagen deposition in the liver, and reduced hepatocyte necrosis. In contrast, knockdown of PTEN expression had an opposite effect, such as increased collagen deposition in the liver, and was molecularly characterized by the increased expression of matrix metalloproteinase(MMP)-13(P < 0.01) and MMP-2(P < 0.01), as well as decreased expression of the tissue inhibitor of metalloproteinase(TIMP)-1(P < 0.01) and TIMP-2(P < 0.01).CONCLUSION These data indicated that gene therapy using recombinant adenovirus encoding PTEN might be a novel way of treating hepatic fibrosis.

关键词： Collagen metabolism Hepatic stellate cells Phosphatase and tension homologue deleted on chromosome ten PTEN Gene therapy Hepatic fibrosis

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Correlation between crowdedness in emergency departments and anxiety in Chinese patients

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World Journal of Clinical Cases 2020年第13期8卷 2802-2816页

作者： Shuang Wang Jun-Yi Gao xiang Li Yu Wu xiao-xia huo Chao-xia Han Meng-Jie Kang Hong Sun Bao-Lan Ge Yu Liu Ying-Qing Liu Jian-Ping Zhou Zhen Wang Department of Emergency Medicine Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Cardiology Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Emergency Intensive Care Unit Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Orthopedic Surgery Beijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Emergency Medicine Peking University People’s HospitalBeijing 100044China Department of Emergency Medicine Peking University Third HospitalBeijing 100191China Department of Emergency Medicine Chinese PLA General HospitalBeijing 100853China Department of Emergency Medicine Beijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China

BACKGROUND Emergency department(ED)overcrowding is a severe health care concern,while anxiety and depression rates among ED patients have been reported to be substantially higher compared to the general *** hypothesized that anxiety due to over crowdedness may lead to adverse events in *** To investigate correlations between crowdedness in EDs and anxiety of patients and nurses,and to identify factors affecting their *** In this prospective observational study,a total 43 nurses and 389 emergency patients from two tier III hospitals located in Beijing were included from January 2016 to August *** were grouped into inpatients when they were hospitalized after diagnoses,or into outpatients when they were discharged after *** State Trait Anxiety Inventory(STAI Form Y)questionnaire was used to investigate patient and nurse anxieties,while crowdedness of EDs was evaluated with the National Emergency Department Over Crowding *** The present results revealed that state anxiety scores(49.50±6.00 vs 50.80±2.80,P=0.005)and trait anxiety scores(45.40±5.70 vs 46.80±2.70,P=0.002)between inpatients(n=173)and outpatients(n=216)were significantly different,while the state anxiety of nurses(44.70±5.80)was different from those of both patient *** linear regression analysis demonstrated that multiple factors,including crowdedness in the ED,were associated with state and trait anxieties for both inpatients and *** addition,there was an interaction between state anxiety and trait ***,multivariable regression analysis showed that while overcrowding in the ED did not directly correlate with patients’and nurses’anxiety levels,the factors that did correlate with state and trait anxieties of inpatients were related to *** factors included waiting time in the ED,the number of patients treated,and the number of nurses in the ED,whereas for nurses,only state and trait anxieties correlat

关键词： Emergency department Overcrowding State anxiety Trait anxiety Anxiety Prospective studies

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FAK-ERK信号转导通路在FRNK抑制肝星状细胞胶原合成中的作用

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世界华人消化杂志 2009年第14期17卷 1402-1405页

作者：申建刚张晓岚魏娟霍晓霞桑荣霞安君艳深圳市人民医院广东省深圳市518003 河北医科大学第二医院消化科河北省石家庄市050000 石家庄市人民医院消化科河北省石家庄市050000

目的:探讨FAK-ERK信号转导通路在黏着斑激酶相关非激酶(FAK-related non-kinase,FRNK)质粒转染抑制肝星状细胞(hepatic stellate cells,HSCs)胶原合成中的作用.方法:在体外,以FN诱导HSCs增殖,采用脂质体介导的方法用FRNK表达质粒瞬时转... 详细信息

目的:探讨FAK-ERK信号转导通路在黏着斑激酶相关非激酶(FAK-related non-kinase,FRNK)质粒转染抑制肝星状细胞(hepatic stellate cells,HSCs)胶原合成中的作用.方法:在体外,以FN诱导HSCs增殖,采用脂质体介导的方法用FRNK表达质粒瞬时转染HSCs,利用3H-Pro掺入技术测定HSCsⅠ型胶原的合成,Western blot及RT-PCR方法检测FRNK、FAK、p-FAK(Tyr397)、ERK蛋白和mRNA的表达.结果:FRNK表达质粒成功转染HSCs,在翻译后水平抑制FAK磷酸化.在FRNK转染HSC48h后胶原合成能力较空质粒组显著下降(498.17±73.20vs748.33±61.30,P<0.01).FRNK抑制FAK磷酸化和在翻译和转录水平抑制ERK1、p-ERK的表达,而FN则促进FAK和ERK1、p-ERK在翻译和转录水平的表达.结论:FRNK可以使HSCs胶原合成能力降低,FAK-ERK信号转导通路可能发挥了负调控作用.

关键词：肝星状细胞黏着斑激酶相关非激酶细胞外信号调节激酶胶原免疫蛋白印迹

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门静脉-肠系膜上静脉广泛栓塞1例并文献复习

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世界华人消化杂志 2019年第23期27卷 1460-1464页

作者：霍晓霞牛巍巍赵亦雯张晓岚河北医科大学第二医院消化内科

背景内镜下套扎术是食管胃静脉曲张出血的一线治疗方法.食管胃静脉曲张密集套扎后形成门静脉-肠系膜上静脉广泛新发血栓临床罕见.病例简介本文回顾性分析1例乙肝肝硬化脾切除术后食管胃静脉曲张出血患者,经食管胃底曲张静脉密集套扎治... 详细信息

背景内镜下套扎术是食管胃静脉曲张出血的一线治疗方法.食管胃静脉曲张密集套扎后形成门静脉-肠系膜上静脉广泛新发血栓临床罕见.病例简介本文回顾性分析1例乙肝肝硬化脾切除术后食管胃静脉曲张出血患者,经食管胃底曲张静脉密集套扎治疗后出现门静脉-肠系膜上静脉广泛新发血栓,积极给予抗凝治疗后,新发血栓消失.结论对于肝硬化脾切除术后食管胃静脉曲张出血,尤其同时存在慢性门静脉血栓者,如需行套扎治疗,勿使套扎范围过密,不要追求一次性全部阻断血流,以防门静脉-肠系膜上静脉新发血栓形成.

关键词：肝硬化静脉曲张密集套扎门静脉肠系膜上静脉血栓

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Potential dependence in electrocatalysis: a theoretical perspective

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Science China Chemistry 2025年第1期 21-25页

作者： Leyu Liu Zhaoming xia Zeyu Wang Yinjuan Chen Hai xiao Department of Chemistry Tsinghua University Fundamental Science Center of Rare Earths Ganjiang Innovation Academy Chinese Academy of Sciences Key Laboratory of Advanced Catalytic Materials and Technology Advanced Catalysis and Green Manufacturing Collaborative Innovation Center Changzhou University

Electrocatalysis is a key technology for the new energy era and sustainable development, serving as the interface for interconversion of electrical and chemical energies, which spans a wide spectrum of applications in sustainable energy and environmental engineering. Electrocatalytic reactions including electrochemical CO2reduction reaction （eCO2RR）, hydrogen evolution reaction （HER）, and oxygen evolution/reduction reactions （OER/ORR）, hold essential promise for renewable energy technologies as well as complement the biogeochemical carbon and oxygen cycles.

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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu Yan Yu xiao-Li Li xiao-Ting Sun Li-Ting Wang xia Wu Ling-Jie Zhu xiao-Jia Yang Qian-Ru He Fei Ding Hai-Yan Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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Chondroitinase ABC combined with Schwann cell transplantation enhances restoration of neural connection and functional recovery following acute and chronic spinal cord injury

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Neural Regeneration Research 2025年第5期20卷 1467-1482页

作者： Wenrui Qu xiangbing Wu Wei Wu Ying Wang Yan Sun Lingxiao Deng Melissa Walker Chen Chen Heqiao Dai Qi Han Ying Ding Yongzhi xia George Smith Rui Li Nai-Kui Liu xiao-Ming Xu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteDepartment of Neurological SurgeryIndiana University School of MedicineIndianapolisINUSA Department of Hand Surgery the Second Hospital of Jilin UniversityChangchunJilin ProvinceChina Shriners Hospitals Pediatric Research Center Temple University School of MedicinePhiladelphiaPAUSA Department of Anatomy&Cell Biology Indiana University School of MedicineIndianapolisINUSA

Schwann cell transplantation is considered one of the most promising cell-based therapy to repair injured spinal cord due to its unique growth-promoting and myelin-forming properties.A the Food and Drug Administration-approved Phase I clinical trial has been conducted to evaluate the safety of transplanted human autologous Schwann cells to treat patients with spinal cord injury.A major challenge for Schwann cell transplantation is that grafted Schwann cells are confined within the lesion cavity,and they do not migrate into the host environment due to the inhibitory barrier formed by injury-induced glial scar,thus limiting axonal reentry into the host spinal *** we introduce a combinatorial strategy by suppressing the inhibitory extracellular environment with injection of lentivirus-mediated transfection of chondroitinase ABC gene at the rostral and caudal borders of the lesion site and simultaneously leveraging the repair capacity of transplanted Schwann cells in adult rats following a mid-thoracic contusive spinal cord *** report that when the glial scar was degraded by chondroitinase ABC at the rostral and caudal lesion borders,Schwann cells migrated for considerable distances in both rostral and caudal *** Schwann cell migration led to enhanced axonal regrowth,including the serotonergic and dopaminergic axons originating from supraspinal regions,and promoted recovery of locomotor and urinary bladder ***,the Schwann cell survival and axonal regrowth persisted up to 6 months after the injury,even when treatment was delayed for 3 months to mimic chronic spinal cord *** findings collectively show promising evidence for a combinatorial strategy with chondroitinase ABC and Schwann cells in promoting remodeling and recovery of function following spinal cord injury.

关键词： axonal regrowth bladder function chondroitinase ABC functional recovery glial scar lentivirus migration Schwann cell spinal cord injury transplantation

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Orbitronics for energy-efficient magnetization switching

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Science China(Information Sciences) 2025年第1期 398-399页

作者： Yuxuan YAO Daoqian ZHU Qingtao xia Jianing LIANG Yuhao JIANG Zhiyang PENG Jiaxu LI Chen xiao Renyou XU Wenwen WANG xiantao SHANG Shiyang LU Dapeng ZHU Hong-Xi LIU Kaihua CAO Weisheng ZHAO Fert Beijing Institute School of Integrated Circuit Science and Engineering Beihang University National Key Lab of Spintronics Institute of International Innovation Beihang University Qingdao Research Institute Beihang University Truth Memory Corporation

Spin-orbit torque （SOT）,as a promising writing method for magnetic random-access memory （MRAM）,has garnered widespread interest for over a decade [1].Heavy metals,such as β-W,have been broadly adopted as spin current sources,but suffer from phase change at a limited thickness,resulting in a dramatic reduction of the charge-tospin conversion efficiency θSH[2].The thickness limitation leads to stringent etching conditions and large distribution among devices,posing challenges for simultaneously satisfying SOT efficiency,etching-stop margins,and back-end-ofline （BEOL） compatibility.

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Managing Procurement and Production Outsourcing under Dynamic Prices

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Acta Mathematicae Applicatae Sinica 2013年第1期29卷 135-142页

作者： xiao-gang CAO Chao YANG Hui WEN huo-song xia Ji-zi LI School of Management Huazhong University of Science and Technology School of Management Wuhan Textile University School of Science Hubei University of Technology

This paper proposes a procurement and production outsourcing model subject to a dynamic prices environment. Using the optimal control theory we obtain the necessary conditions of the optimal procurement and production policy. From the study of optimal control conditions, we derive qualitative properties of the optimal procurement and production decisions for a few exemplifying cases. Through these results we are able to provide some managerial implications for managers to make real decisions.

关键词： production planning dynamic prices optimal control production outsourcing

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Research on key technologies of edge cache in virtual data space across WAN

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Frontiers of Computer Science 2023年第1期17卷 15-34页

作者： Jiantong huo Yaowen XU Zhisheng huo Limin xiao Zhenxue HE State Key Laboratory of Software Development Environment Beihang UniversityBeijing 100191China School of Computer Science and Engineering Beihang UniversityBeijing 100191China High Performance Computing Center Beihang UniversityBeijing 100191China College of Software Beihang UniversityBeijing 100191China Hebei Key Laboratory of Agricultural Big Data Hebei Agricultural UniversityBaoding 071001China College of Computer Science and Technology Zhejiang UniversityHangZhou 310013China

The authors of this paper have previously proposed the global virtual data space system (GVDS) to aggregate the scattered and autonomous storage resources in China’s national supercomputer grid (National Supercomputing Center in Guangzhou, National Supercomputing Center in Jinan, National Supercomputing Center in Changsha, Shanghai Supercomputing Center, and Computer Network Information Center in Chinese Academy of Sciences) into a storage system that spans the wide area network (WAN), which realizes the unified management of global storage resources in China. At present, the GVDS has been successfully deployed in the China National Grid environment. However, when accessing and sharing remote data in the WAN, the GVDS will cause redundant transmission of data and waste a lot of network bandwidth resources. In this paper, we propose an edge cache system as a supplementary system of the GVDS to improve the performance of upper-level applications accessing and sharing remote data. Specifically, we first designs the architecture of the edge cache system, and then study the key technologies of this architecture: the edge cache index mechanism based on double-layer hashing, the edge cache replacement strategy based on the GDSF algorithm, the request routing based on consistent hashing method, and the cluster member maintenance method based on the SWIM protocol. The experimental results show that the edge cache system has successfully implemented the relevant operation functions (read, write, deletion, modification, etc.) and is compatible with the POSIX interface in terms of function. Further, it can greatly reduce the amount of data transmission and increase the data access bandwidth when the accessed file is located at the edge cache system in terms of performance, i.e., its performance is close to the performance of the network file system in the local area network (LAN).

关键词： virtual data space system wide area network edge cache redundant data transmission

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