检索结果-南通市图书馆

原子力显微镜观察体外纯化蛋白分子的表面结构

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国药理学通报 2025年第1期41卷 193-199页

作者：陈昭颖方骁枫刘丹彦陈梦琴凌夕赵洁旻魏伟吴育晶安徽医科大学临床药理研究所抗炎免疫药物教育部重点实验室安徽合肥230032 安徽医科大学第二临床医学院安徽合肥230022

目的以课题组纯化获得的蛋白样本为观察对象,对比原子力显微镜(atomic force microscope,AFM)及扫描电子显微镜(scanning electron microscope,SEM)的观察结果,并总结AFM观察生物大分子的主要问题和解决方法。方法将蛋白样本使用PBS稀释... 详细信息

目的以课题组纯化获得的蛋白样本为观察对象,对比原子力显微镜(atomic force microscope,AFM)及扫描电子显微镜(scanning electron microscope,SEM)的观察结果,并总结AFM观察生物大分子的主要问题和解决方法。方法将蛋白样本使用PBS稀释至15 nmol·L^(-1),分别固定于载玻片、硅片和云母片上烘干,制成固相观察样本,SEM样本在观察前镀铂,使用AFM及SEM观察蛋白质表面结构,计算样本高度,对比结果差异。结果带正电的蛋白样本在观察时由于AFM探针的斥力会向右偏移;云母片能很好地消除蛋白正电荷从而避免样本移动;PBS能为蛋白样本提供良好的稳定环境,但PBS盐结晶会干扰探针运行和成像清晰度;SEM样本需要镀铂后观察,无法达到AFM的精度。结论使用AFM和SEM均可在体外环境直接观察蛋白质结构,AFM能提供更高精度的观察结果;在蛋白样本稳定性允许的情况下首选超纯水为溶剂载体,乙醇等挥发性液体也可作为溶剂载体,AFM的应用可为药理学生物大分子互作研究提供一新途径。

关键词：原子力显微镜扫描电子显微镜受体结构蛋白质体外结构超微结构云母载体

Effect of C/N Ratio on Nitrogen Removal of A/O-MBBR Process for Treating Mariculture Wastewater

在线全文

学校读者我要写书评

暂无评论

Journal of Ocean University of China 2021年第4期20卷 879-885页

作者： CHEN xi xiANG Zhuangzhuang HUANG xiao RONG Huimin BAI Jie zhao Yangguo College of Marine Life Science Ocean University of ChinaQingdao 266003China College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Nanjing University of Information Science and Technology Collaborative Innovation Center of Atmospheric Environment and Equipment TechnologyJiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution ControlNanjing 210044China Key Laboratory of Marine Environment and Ecology(Ocean University of China)Ministry of Education Qingdao 266100China

In order to explore the effect of carbon and nitrogen(C/N)ratio on the performance of anoxic/aerobic-moving bed bio-film reactor(A/O-MBBR)process for treating mariculture wastewater,a laboratory-scale A/O-MBBR was *** results showed that the reduction of C/N ratio was conducive to improving the removal efficiency of chemical oxygen demand(COD)and ammonia nitrogen(NH_(4)^(+)-N),while inhibiting that of nitrite nitrogen(NO_(2)^(−)-N)and nitrate nitrogen(NO_(3)^(−)-N).The extracellular polymeric substances(EPS)in anoxic zone were significantly higher in concentration than that in aerobic zone although they both declined with decrease of C/N *** result provides solid support for better controlling the pollution of mariculture wastewater.

关键词： mariculture wastewater C/N ratio A/O-MBBR process EPS

Distribution of oxide inclusions in H13 castings under super-gravity field with multi-stage rotation speeds

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2024年第1期31卷 121-133页

作者： Shao-ying Li xiao-jun xi xing-ming zhao Han-jie Guo Jing Guo School of Metallurgical and Ecological Engineering University of Science and Technology Beijing(USTB)Beijing100083China Beijing Key Laboratory of Special Melting and Preparation of High-End Metal Materials Beijing100083China Beijing Beiye Functional Materials Corporation Beijing100192China Shandong Tianwu Forming Technology Co. Ltd.Liaocheng252024ShandongChina

The 42 kg industrial H13 castings were prepared by different super-gravity fields with multi-rotation speeds,and the distribution of oxide inclusions in the castings was *** addition,the inward movement Reynolds number and inward movement time of oxide inclusions as well as the solidification time of molten steel at different positions in the castings were calculated to clarify the removal mechanism of oxide inclusions in super-gravity *** results show that the large size(i.e.,greater than 10μm)oxide inclusions are mainly concentrated in the inner and outer parts of the super-gravity castings with constant rotation speed(500 r min^(-1))and five-stage rotation speeds(500,600,750,850,and 950 r min^(-1)),respectively,while there are no large oxide inclusions in the super-gravity castings with three-stage rotation speeds(500,600,and 750 r min^(-1)).Although an increase in the particle size of inclusion and the rotation speed in super-gravity field is conducive to the increase in the inward movement Reynolds number of oxide inclusions and reduction in the inward movement time of oxide inclusions,it will reduce the local solidification time of molten *** the range of the rotation speed studied,the super-gravity field with three-stage rotation speeds has the best effect on the removal of inclusions in H13 molten steel.

关键词： Distribution Oxide inclusion H13 casting Super-gravity field Rotation speed

Effects of combined antibiotics on nitrification, bacteria and antibiotic resistance genes in activated sludge: Insights from legacy effect of antibiotics

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2023年第9期131卷 96-110页

作者： Zhong-xing Zhang xiao-Yan Fan xing Li Yu-xi Gao Jun-Ru zhao Faculty of Architecture Civil and Transportation EngineeringBeijing University of TechnologyBeijing 100124China

The effect of combined antibiotics exposure on nitrogen removal,microbial community assembly and proliferation of antibiotics resistance genes(ARGs)is a hotspot in activated sludge ***,it is unclear that how the historical antibiotic stress affects the subsequent responses of microbes and ARGs to combined *** this study,the effects of combined sulfamethoxazole(SMX)and trimethoprim(TMP)pollution on activated sludge under legacy of SMX or TMP stress with different doses(0.005-30 mg/L)were investigated to clarify antibiotic legacy *** activity was inhibited under higher level of combined exposure but a high total nitrogen removal(∼70%)*** on the full-scale classification,the legacy effect of past antibiotic stress had a marked effect on community composition of conditionally abundant taxa(CAT)and conditionally rare or abundant taxa(CRAT).Rare taxa(RT)were the keystone taxa in the microbial network,and the responses of hub genera were also affected by the legacy of antibiotic *** bacteria and genes were inhibited by the antibiotics and aerobic denitrifying bacteria(Pseudomonas,Thaurea and Hydrogenophaga)were enriched under legacy of high dose,as were the key denitrifying genes(napA,nirK and norB).Furthermore,the occurrences and co-selection relationship of 94 ARGs were affected by legacy ***,some shared hosts(eg.,Citrobacter)and hub ARGs(eg.,mdtD,mdtE and acrD)were ***,antibiotic legacy could affect responses of activated sludge to combined antibiotic and the legacy effect was stronger at higher exposure levels.

关键词： Antibiotics Antibiotic resistance genes(ARGs) Combined pollution Full-scale classification Legacy effect

Towards Positivity:A Large-Scale Diachronic Sentiment Analysis of the Humanities and Social Sciences in China

在线全文

学校读者我要写书评

暂无评论

Fudan Journal of the Humanities and Social Sciences 2023年第4期16卷 569-589页

作者： Wei xiao Yuxin Guo xi zhao School of Foreign Languages and Cultures Chongqing UniversityChongqingChina Research Center for Language Cognition and Language ApplicationChongqing UniversityChongqingChina

With its rising number of publications and expanding international collaborations,China's humanities and social sciences(HSS)research is displaying its potential for global *** have been exploring the development of China's HSS from different ***,the examinations from the perspective of sentiment analysis are *** aim is then to examine the sentiment features in Chinese HSS academic writing,by analyzing a large-scale corpus with over 275 million characters and with a time span from 2000 to *** that most studies only focused on abstracts,we examined both the abstracts and the full texts,as well as a direct comparison between *** found that Chinese HSS academic writing has evolved to be more positively biased in the past two decades,showing an upward trend in the use of positive words and a slight downward trend in the use of negative ***,the upward trend of positive words in the full texts is not that clear,resembling a fluctuating *** the comparison,the abstracts are more likely to use positive words while the full texts tend to use more negative *** phenomena can be explained with the social cognitive theory,in that they may be shaped by a joint force of the nature of human beings,the nature of language,the particular socio-cultural background in China and the features of the academic genre.

关键词： China Humanities and social sciences Academic writing Linguistic positivity bias Sentiment analysis

冷冻联合局部药物灌注治疗淋巴结瘘型气管支气管结核的结果分析

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国防痨杂志 2017年第3期39卷 256-259页

作者：肖阳宝席钊罗林紫罗莉陈璐李芳白湖南省胸科医院内镜诊疗科长沙410013 湖南省胸科医院呼吸内科长沙410013 湖南省胸科医院结核科长沙410013

目的对支气管镜下冷冻联合局部药物灌注治疗淋巴结瘘型气管支气管结核的临床结果进行分析.方法回顾性分析2012年9月至2014年3月在湖南省胸科医院内镜诊疗科接受冷冻联合局部药物灌注治疗的53例淋巴结瘘型气管支气管结核的住院患者,分... 详细信息

目的对支气管镜下冷冻联合局部药物灌注治疗淋巴结瘘型气管支气管结核的临床结果进行分析.方法回顾性分析2012年9月至2014年3月在湖南省胸科医院内镜诊疗科接受冷冻联合局部药物灌注治疗的53例淋巴结瘘型气管支气管结核的住院患者,分析治疗的次数、治疗时间、治疗效果及并发症,采用秩和检验对单发和多发病灶患者治疗疗程和效果进行统计学分析,以P＜0.05为差异有统计学意义.结果冷冻治疗的中位次数为6（1～13）次,中位治疗时间为50（7～104）d,局部药物灌注治疗中位次数为8（2～18）次,中位治疗时间为85（14～142）d,总的治疗有效率达到100.00％（53/53）.34例单发病灶患者治疗中位时间为85（14～130）d,完全有效例数为27例,部分有效例数为6例,轻度有效例数为1例;19例多发病灶患者治疗中位时间为84（21～142）d,完全有效例数为13例,部分有效例数为4例,轻度有效例数为2例.单发病灶与多发病灶治疗疗程及疗效差异均无统计学意义（Z=0.26,P=0.785;Z=1.53,P=0.120）.13例患者治疗过程中有少量出血（＜10 ml）,本组患者手术后未出现支气管纵隔瘘、纵隔气肿、大出血（≥100 ml）等严重并发症.结论冷冻联合局部药物灌注治疗淋巴结瘘型气管支气管结核安全、有效,值得推广.

关键词：结核气管疾病支气管瘘支气管镜结核,淋巴结

Preparation of Titanium-silphenylene-siloxane Hybrid Polymers with High Refractive Index,Transmittance,and Thermal Stability

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2020年第9期38卷 973-982,I0007页

作者： Dong zhao Shu-xi Shan Meng Zhang xiao-A Zhang Sheng-Ling Jiang Ya-Fei Lyu Key Laboratory of Carbon Fiber and Functional Polymers of Ministry of Education College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China

Vinyl-containingtitanium-silphenylene-sioxane oligomers(O1,O2,O3,andO4)withdifferent molar ratios of titanium to silicon were successfully synthesized in high yields by nonhydrolytic sol-gel reaction,and the obtained four oligomers were further crosslinked with methylphenyl hydro-silicone oil(4)to form corresponding polymers(P1,P2,P3,and P4)by hydrosilylation *** structures were characterized with spectroscopic characterization techniques including FTIR,1H-NMR,and Raman *** effect of titanium element on crosslinking behavior,thermal resistance,refractive index,transparency,thermal aging stability,glass transition temperature,and surface properties of the polymers were *** with titanium-free polymer P1,the thermal resistance,refractive index,and thermal aging stability of titanium-silphenylene-siloxane polymers(P2,P3,and P4)were significantly *** titanium-silphenylene-siloxane polymers achieved a high refractive index(n=1.580-1.584)and thermostability(Tsd>500℃).In thermal aging,the polymers exhibited superior performances with high optical transparency(~90%at 450 nm)and exhibited high thermal stability(~84%at 450 nm after thermal aging at 150℃for 120 h).These data indicate that the polymers have potential application in optical materials such as LED encapsulants.

关键词： Titanium-silphenylene-siloxane hybrid polymers High refractive index and transmittance Thermal stability

Reversible Chain Transfer Catalyzed Polymerization with Alkyl Iodides Generated from Alkyl Bromides by in Situ Halogen Exchange

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 2021年第9期39卷 1161-1168页

作者： Dan-Ni Gao Yu-Lai zhao Jing-Yu Cai Lin-xi Hou Long-Qiang xiao Department of Materials-Oriented Chemical Engineering School of Chemical EngineeringFuzhou UniversityFuzhou 350116China Qingyuan Innovation Laboratory Quanzhou 362801China Fujian Key Laboratory of Advanced Manufacturing Technology of Specialty Chemicals Fuzhou UniversityFuzhou 350116China

Reversible chain transfer catalyzed polymerization(RTCP)is a practical and efficient process for the precise synthesis of polymers with special architecture by using simple phenols(2,4,6-trimethylphenol,TMP)or hydrocarbons(xanthene,XT)as efficient ***,alkyl iodide(R-1),which was gen erated from in situ bromine-iodine transformation of alkyl bromide(R-Br)with sodium iodide(Nal),was served as initiator to mediate RTCP with TMP or *** and other functional methacrylates,including GMA,DEAM,DMAEMA and BzMA,were successfully initiated by combining orga no catalysts and azo in itiators to yield polymers with low-polydispersity(M_(w)/M_(n)=1.1-1.5)and ideal mono mer conversions(50%-90%)at moderate *** over,3-armstar polymers were also obtained by this *** high chain-end fidelity of the obtained poly(methyl methacrylate)with iodine as chain-end group(PMMA-I)was confirmed by chain-extension *** en vironme ntally frie ndly initiators and orga no catalysts exhibit powerful polymerization properties toward RTCP,providing a sign ificant method to synthesize functional polymers.

关键词： Reversible chain transfer catalyzed polymerization Organocatalyst Halogen exchange

Fabrication of organically modified oxygen sensing film based on fluorescent quenching

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2007年第2期52卷 188-193页

作者： xiN LingLing xiao LaiLong zhao Li CHEN xi WANG xiaoRu Department of Chemistry and the Key Laboratory of Analytical Sciences of Ministry of Education College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005 China State Key Laboratory of Marine Environmental Science Xiamen University Xiamen 361005 China

An organically modified silicate (ORMOSIL) as a matrix for oxygen-sensitive sensor, in which di-methyldimethoxysilane was selected as an organic modifier in the precursor, is described. The sensing film with tris-(4,7-diphenyl-1,10-phenanthroline) ruthenium(II) as an indicator developed in this paper was characterized by efficient quenching by oxygen. Blue light-emitting diodes (λmax = 475 nm) were employed as light excitation source. The linear range of the dissolved oxygen was from 0.5 to 16 μg/mL. The measured RSD was 2%, the response time (t95) was 60 s, and the determination limit was 0.2 μg/mL. A portable and inexpensive luminescence-based sensor was established and applied to the determina-tion of dissolved oxygen in the surface water.

关键词：改性有机硅钌邻二氮杂菲发光