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Evaluation of genetically modified rice detection methods 2011/884/EU and 2008/289/EC proposed by the European Union

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Journal of Integrative Agriculture 2016年第12期15卷 2899-2910页

作者： xiao qi-sheng XU Wen-tao YANG Jie-lin PAN Liang-wen Technical Center for Animal Plant and Food Inspection and QuarantineShanghai Entry and Exit Inspection and Quarantine Bureau College of Food Science & Technology Shanghai Ocean University Beijing Laboratory for Food Quality and Safety/College of Food Science and Nutritional Engineering China Agricultural University

Increases in the number of cases of identified genetically modified （GM） rice contamination can be traced back to the first Rapid Alert System for Food and Feed （RASFF） in 2006. In response to the lack of reliable detection methods, Decision 2011/884/EU proposed that new screening methods replace Decision 2008/289/EC, to identify all possible GM rice products originating in China. However, the synergy brands （SYBR） Green real-time PCR assay proposed by Decision 2011/884/EU has been shown to lack conformity with other TaqMan methods currently in use. To evaluate the specificity and repeatability of the methods recommended in Decision 2011/884/EU and Decision 2008/289/EC, we collected 74 rice products originating from six countries or districts. The 74 rice samples were tested using the Decision 2011/884/EU and Decision 2008/289/ EC methods. The parallel use of different instruments and reagents were used for testing in parallel, and the results were analyzed statistically. To avoid the limitations of specific laboratories, eight GM organism detection laboratories in China participated in a collaborative trial. In our tests, 24.3% （18/74） of the samples tested were positive with the SYBR Green real-time PCR assay using the Decision 2011/884/EU method, but were negative with the TaqMan real-time PCR assay using the Decision 2011/884/EU and Decision 2008/289/EC methods. Sequencing the PCR-amplified CrylA（b/c） genes in three samples （6, 30 and 43） showed that the products consisted of primer dimers rather than the targeted sequence. The combined experimental results showed that testing for the nopaline synthase gene （NOS） of Agrobacterium tumefasciens terminator and CrylA（b/c） produced false-positive results when the Decision 2011/884/EU method was used. Because of the high rate of false-positive results, the Decision 2011/884/EU SYBR Green method to detect GM rice requires improvement.

关键词： genetically modified organism Decision 2011/884/EU SYBIR Green real-time PCR false positive

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Drug-eluting fully covered self-expanding metal stent for dissolution of bile duct stones in vitro

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World Journal of Gastroenterology 2019年第26期25卷 3370-3379页

作者： Chao Huang xiao-Bo Cai Li-Li Guo xiao-sheng qi qiang Gao Xin-Jian Wan Department of Gastroenterology Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 201620China Shanghai Key Laboratory for Pancreatic Diseases Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 201620China Department of General Surgery Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai 201620China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai 201620China

BACKGROUND The treatment of difficult common bile duct stones(CBDS)remains a big challenge around the *** stenting is a widely accepted rescue method in patients with failed stone extraction under endoscopic retrograde *** covered self-expanding metal stent(FCSEMS)has gained increasing attention in the management of difficult *** To manufacture a drug-eluting FCSEMS,which can achieve controlled release of stone-dissolving agents and speed up the dissolution of *** Customized covered nitinol stents were *** cholate(SC)and disodium ethylene diamine tetraacetic acid(EDTA disodium,EDTA for short)were used as stone-dissolving *** different types of drug-eluting stents were manufactured by dip coating(Stent I),coaxial electrospinning(Stent II),and dip coating combined with electrospinning(Stent III),*** drugrelease behavior and stone-dissolving efficacy of these stents were evaluated in vitro to sort out the best manufacturing *** the selected stonedissolving stents were further put into porcine CBD to evaluate their *** Stent I and Stent II had obvious burst release of drugs in the first 5 d while Stent III presented controlled and sustainable drug release for 30 *** still buffer,the final stone mass-loss rate of each group was 5.19%±0.69%for naked FCSEMS,20.37%±2.13%for Stent I,24.57%±1.45%for Stent II,and 33.72%±0.67%for Stent *** flowing bile,the final stone mass-loss rate of each group was 5.87%±0.25%for naked FCSEMS,6.36%±0.48%for Stent I,6.38%±0.37%for Stent II,and 8.15%±0.27%for Stent *** III caused the most stone mass-loss no matter in still buffer or in flowing bile,which was significantly higher than those of other groups(P0.05)and histopathological examination(P>0.05).CONCLUSION The novel SC and EDTA-eluting FCSEMS is efficient in diminishing CBDS in ***

关键词： Common bile duct stone Drug-eluting stent Fully covered self-expanding metal stent Electrospinning Nanofiber film

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Expert consensus on management of metabolic disease in Chinese liver transplant recipients

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World Journal of Gastroenterology 2020年第27期26卷 3851-3864页

作者： Tian Shen Li Zhuang xiao-Dong Sun xiao-sheng qi Zhi-Hui Wang Rui-Dong Li Wen-Xiu Chang Jia-Yin Yang Yang Yang Shu-Sen Zheng xiao Xu Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003Zhejiang ProvinceChina Department of Hepatobiliary and Pancreatic Surgery Shulan(Hangzhou)HospitalHangzhou 310003Zhejiang ProvinceChina Department of Hepatobiliary and Pancreas Surgery The First HospitalJilin UniversityChangchun 130021Jilin ProvinceChina Department of General Surgery Shanghai First People's HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200080China Department of Hepatobiliary and Pancreatic Surgery The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052Henan ProvinceChina Department of General Surgery and Liver Transplant Center Huashan HospitalFudan UniversityShanghai 200040China Department of Nephrology Tianjin First Center HospitalTianjin 300384China Department of Liver Surgery and Liver Transplantation Center West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Hepatic Surgery and Liver Transplantation Center The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina

Metabolic disease,including diabetes mellitus,hypertension,dyslipidemia,obesity,and hyperuricemia,is a common complication after liver transplantation and a risk factor for cardiovascular disease and *** development of metabolic disease is closely related to the side effects of ***,optimization of the immunosuppressive regimen is very important for the prevention and treatment of metabolic *** Chinese Society of Organ Transplantation has developed an expert consensus on the management of metabolic diseases in Chinese liver transplant recipients based on recent *** is placed on the risk factors of metabolic diseases,the effect of immunosuppressants on metabolic disease,and the prevention and treatment of metabolic diseases.

关键词： Liver transplantation Metabolic disease Diabetes mellitus Hypertension Dyslipidemia Hyperuricemia Obesity Immunosuppressive agents Consensus

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Cross-sectional study of congenital heart disease among Tibetan children aged from 4 to 18 years at different altitudes in qinghai Province

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Chinese Medical Journal 2008年第24期121卷 2469-2472页

作者： CHEN qiu-hong WANG xiao-qin qi sheng-gui Central Laboratory Qinghai Cardiovascular Diseases Vocational Hospital Xining Qinghai 810012 China

Background Congenital heart disease （CHD） is a common heart condition which does considerable harm to the health of children and adolescents. CHD epidemiological characteristics of Tibetan children whose ages ranged from 4 to 18 years were investigated in qinghai Province. Methods A total of 32 578 Tibetan children, living at altitudes of 2535 m, 3600 m and 4200 m, were examined using a three-stage protocol： prescreening, rechecking and diagnosis using a color Doppler. The distribution of CHD at different altitudes was analyzed together with differences in occurrence according to age and gender. Results A total of 235 CHD cases were discovered. The total prevalence of CHD was 7.21‰. Prevalence of CHD has been shown to increase along with increase in altitude with 5.45‰ at an altitude of 2535 m, 6.80‰ at 3600 m and 9.79‰ at 4200 m. There were no statistically significant differences between the prevalence at 2535 m and 3600 m （Х^2=1.594, P 〉0.05）. However, there was a significant difference between the prevalence at 2535 m and 4200 m （Х^2=7.002, P 〈0.01）. Also, apparent differences existed between the prevalence at 3600 m and at 4200 m （Х^2=5.540, P 〈0.05）. There was no statistically significant difference in prevalence according to age at an altitude of 2535 m, but the rate of CHD increased significantly along with increasing age at 3600 m and 4200 m. The total prevalence ratio of children aged from 16 to 18 years was significantly higher than that of children from 4 to 7, and from 8 to 12 with Х^2 values of 10.79 （P 〈0.005）, and 5.60 （P 〈0.05） respectively. Within the constituent ratio of CHD, the prevalence of atrial septal defect （ASD） was the highest at 39.10%, followed by the prevalence of ventricular septal defect （VSD） at 32.8% and patent ductus arteriosus （PDA） at 24.7%. Furthermore the proportion of the four categories of CHD varied at different altitude levels： at 2535 m, of those diagnosed with CHD, the prevalence rate of VSD was the highest at 43.5%, at

关键词： congenital heart disease epidemiological monitoring prevalence Tibetan

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融合多尺度Transformer的皮肤病变分割算法

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吉林大学学报（工学版） 2024年第4期54卷 1086-1098页

作者：梁礼明周珑颂尹江盛校棋江西理工大学电气工程与自动化学院江西赣州341000 华南理工大学计算机科学与工程学院广东广州510006

针对现有皮肤病变图像分割时缺乏多尺度特征提取,从而导致细节信息缺失和病变区域误分割的问题,本文提出一种融合多尺度Transformer的编解码网络皮肤病变分割算法。首先运用Transformer模块构建分层编码器,分层编码器从全局特征变化角... 详细信息

针对现有皮肤病变图像分割时缺乏多尺度特征提取,从而导致细节信息缺失和病变区域误分割的问题,本文提出一种融合多尺度Transformer的编解码网络皮肤病变分割算法。首先运用Transformer模块构建分层编码器,分层编码器从全局特征变化角度出发,多尺度分析皮肤病变区域;然后利用多尺度融合模块、通道注意力模块和联合层构建融合解码器,多尺度融合模块互补分层编码器中浅层网络信息与深层网络信息,增强空间信息和语义信息间的依赖关系,通道注意力模块能够有效识别特征丰富的通道,提高算法分割精度;最后引入扩展模块恢复图像大小以匹配实际需求。将该算法在ISBI2016、ISBI2017和ISIC2018三个公共数据集上进行实验测试,其像素精度分别为96.70%、94.50%和95.39%,平均交并比分别为91.69%、85.74%和89.29%,算法测试整体性能优于现有算法。仿真实验证明,多尺度Transformer编解码网络能够有效地分割皮肤病变图像。

关键词：计算机应用技术皮肤病变图像分割 Transformer 多尺度融合模块通道注意力模块

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Analysis of Synonymous Codon Usage Bias in 09H1N1

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Virologica Sinica 2010年第5期25卷 329-340页

作者： Zhen-peng LI De-quan YING Peng LI Fei LI xiao-chen BO sheng-qi WANG Beijing Institute of Radiation Medicine Beijing 100850 China

A novel subtype of influenza A virus 09H1N1 has rapidly spread across the world. Evolutionary analyses of this virus have revealed that 09H1N1 is a triple reassortant of segments from swine, avian and human influenza viruses. In this study, we investigated factors shaping the codon usage bias of 09H1N1 and carried out cluster analysis of 60 strains of influenza A virus from different subtypes based on their codon usage bias. We discovered that more preferentially used codons of 09H1N1 are A-ended or U-ended, and the intra-genomic codon usage bias of 09H1N1 is quite low. Base composition constraint, dinucleotide biases and translational selection are the main factors influencing the codon usage bias of 09H1N1. At the genome level, we find that the codon usage bias of 09H1N1 is similar to H1N1 (A/swine/Kansas/77778/2007H1N1), H9N2 from Asia, H1N2 from Asia and North America and H3N2 from North America. Our results provide insight for understanding the processes governing evolution, regulation of gene expression, and revealing the evolution of 09H1N1.

关键词： 09H1N1 Correspondence analysis Codon usage bias

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基于加权特征子空间的支持向量机核函数研究

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科学技术与工程 2020年第15期20卷 6101-6106页

作者：梁礼明郭凯盛校棋江西理工大学电气工程与自动化学院赣州341000

针对数据分类交叉空间易错分问题,提出一种基于加权特征子空间的支持向量机核函数方法。该方法利用加权特征子空间与稀疏表达等相关理论进行支持向量机核函数优化,首先利特征子空间重叠率与数据的信息熵对数据特征进行加权,再通过对L1... 详细信息

针对数据分类交叉空间易错分问题,提出一种基于加权特征子空间的支持向量机核函数方法。该方法利用加权特征子空间与稀疏表达等相关理论进行支持向量机核函数优化,首先利特征子空间重叠率与数据的信息熵对数据特征进行加权,再通过对L1范数正则项的加权处理调节异类数据间的稀疏性和同类数据间的稠密性;最后对处理好的数据进行分类测试。仿真实验表明该算法能够在一定程度上提升分类效果,以达到优化核函数的目的。

关键词：支持向量机 L1范数核函数加权特征子空间信息熵

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一种避免种群聚集的多目标粒子群优化算法

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微电子学与计算机 2015年第7期32卷 86-89,94页

作者：王洋高圣国盛小琪上海工程技术大学管理学院上海201620

为了提高多目标粒子群优化算法的收敛性和分布性,提出了一种改进的粒子群优化算法.该算法在PSO算法的基础上增加了两个操作:一是在种群优化更新阶段进行正态分布投点的变异操作,提高算法的搜索能力.另一个是在外部存储库更新阶段采用先... 详细信息

为了提高多目标粒子群优化算法的收敛性和分布性,提出了一种改进的粒子群优化算法.该算法在PSO算法的基础上增加了两个操作:一是在种群优化更新阶段进行正态分布投点的变异操作,提高算法的搜索能力.另一个是在外部存储库更新阶段采用先进后出的更新方式,即求出的非支配解全部进入外部存储库中,然后剔除距离较近的粒子.测试函数ZDT1-ZDT4的计算结果表明了该算法能有效地提高解的收敛性和分布性.

关键词：粒子群优化算法变异操作更新仿真实验

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Ginsenoside Rg1 inhibits proliferation of vascular smooth muscle cells stimulated by tumor necrosis factor-α

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Acta Pharmacologica Sinica 2006年第8期27卷 1000-1006页

作者： Zeng-chun MA Yue GAO~2 Yu-guang WANG Hong-ling TAN Cheng-rong xiao sheng-qi WANG Institute of Radiation Medicine Sciences,Academy of Military Medical Sciences,Beijing 100850,China Institute of Radiation Medicine Sciences Academy of Military Medical Sciences Beijing 100850 China

Aim:To investigate the proliferation of vascular smooth muscle cells(VSMC)affected by ginsenoside Rg1 and further explore the molecular mechanism ofginsenoside Rg1 using ***:The proliferation of VSMC wasmeasured by MTS assay kit and flow *** alterations were ana-lyzed using two-dimensional electrophoresis and peptide mass *** proteins found in proteomics were confirmed by ***:The proliferation of VSMC was enhanced significantly after tumor necrosis fac-tor-α(TNF-α)treatment,and ginsenoside Rg1 treatment inhibited proliferation ina dose-dependent *** analysis showed 24 protein spots werechanged,including 17 spots that were increased and 7 spots that were *** Rg1 could restore the expression levels of these proteins,at leastpartly,to basic levels of untreated *** expression of G-protein coupledreceptor kinase,protein kinase C(PKC)-ζ,N-ras protein were decreased,whilecycle related protein p21 was increased by ginsenoside Rgl in TNF-α ***:PKC-ζ and p21 pathway might be the mechanism for inhibi-tory effects of ginsenoside Rg1 on proliferation of VSMC.

关键词： cell cycle ginsenoside Rg1 proteomics tumor necrosis factor-α vascular smooth muscle cells

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Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long xiao Gao Zhengwei Wang Zhizhong Guan xiaolan qi Wei Hong Yan xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

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