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Reduced mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor contributes to neurodegeneration in a model of spinal and bulbar muscular atrophy pathology

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Neural Regeneration Research 2025年第9期20卷 2655-2666页

作者： Yiyang qin Wenzhen Zhu Tingting Guo Yiran Zhang Tingting Xing Peng Yin Shihua li xiao-Jiang li Su Yang Guangdong Key Laboratory of Non-human Primate Research Key Laboratory of CNS Regeneration(Ministry of Education)Guangdong–HongKong–Macao Institute of CNS RegenerationJinan UniversityGuangzhouGuangdong ProvinceChina

Spinal and bulbar muscular atrophy is a neurodegenerative disease caused by extended CAG trinucleotide repeats in the androgen receptor gene,which encodes a ligand-dependent transcription facto *** mutant androgen receptor protein,characterized by polyglutamine expansion,is prone to misfolding and forms aggregates in both the nucleus and cytoplasm in the brain in spinal and bulbar muscular atrophy *** aggregates alter protein-protein interactions and compromise transcriptional *** this study,we reported that in both cultured N2a cells and mouse brain,mutant androgen receptor with polyglutamine expansion causes reduced expression of mesencephalic astrocyte-de rived neurotrophic *** n of mesencephalic astrocyte-derived neurotrophic factor amelio rated the neurotoxicity of mutant androgen receptor through the inhibition of mutant androgen receptor *** down endogenous mesencephalic astrocyte-derived neurotrophic factor in the mouse brain exacerbated neuronal damage and mutant androgen receptor *** findings suggest that inhibition of mesencephalic astrocyte-derived neurotrophic factor expression by mutant androgen receptor is a potential mechanism underlying neurodegeneration in spinal and bulbar muscular atrophy.

关键词： androgen receptor mesencephalic astrocyte-derived neurotrophic factor mouse model neurodegeneration neuronal loss neurotrophic factor polyglutamine disease protein misfolding spinal and bulbar muscular atrophy transcription factor

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Clustering dendrograms construction and analysis of TIM-barrel proteins by structure information

Clustering dendrograms construction and analysis of TIM-barr...

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第五届全国生物信息学与系统生物学学术大会暨国际生物信息学前沿研讨会高通量时代的生物信息学与系统生物学

作者： Xin-Rui SONG xiao-qin li Bioengineering Center Beijing University of Technology

Background:The regularity study of protein molecular evolution is the key to molecular evolution and it has important meaning to reveal life origin and the evolutionary mechanism. TIM-barrel fold is a typical fold type and members of this fold have a common topology but with little sequence similarity through *** pairwise amino acid sequence diversity of TIM-barrel fold proteins can be up to 70%,so it is difficult to detect their evolutionary relationship from *** a result of the growing number of protein structure information of PDB and the development of structure alignment algorithms,basing on structure comparison make it better to study TIM-barrel fold proteins than that of sequence. Methods:In this paper,326 TIM-barrel proteins with the spatial structure material and known species information were selected from Astral 1.65(sequence identity lished to construct clustering dendrograms using their structure alignment information,*** species phylogenies of different datasets were obtained from NCBI. Results:The analysis about clustering dendrograms of TIM-barrel protein dataset shows that proteins with similar function obviously tend to *** same superfamily members are almost clustered in a large branch and the same family members are clustered in a small branch belonging to their superfamily’***,there is little relation between clustering dendrograms and their corresponding species *** analysis of other three subsets indicates that under the constraints of function,clustering dendrograms of TIM-barrel proteins have a good corresponding relation with species phylogenies. Conclusions:Our results indicate that the structure evolution of TIM-barrel proteins reflects the constraints of protein function,the structural differences between specific functional TIM-

关键词： TIM-barrel proteins structure alignment hierarchical cluster species evolution

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Values of Donor Serum lipids and Calcium in Predicting Graft Function after Kidney Transplantation:A Retrospective Study

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Current Medical Science 2023年第3期43卷 514-519页

作者： Hui-bo SHI Yuan-yuan ZHAO Yu li Yi li Bin liU Nian-qiao GONG Sheng CHANG Dun-feng DU Lan ZHU Jing XU xiao-qin li Meng-jun ZENG Shang-xin DONG Zhi-shui CHEN Ji-pin JIANG Institute of Organ Transplantation Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyKey Laboratory of Organ TransplantationMinistry of EducationMinistry of Public HealthChinese Academy of Medical SciencesWuhan430030China The Organ Procurement Organizations Ofice Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Objective Delayed graft function(DGF)and early graft loss of renal grafts are determined by the quality of the kidneys from the deceased ***“non-traditional”risk factors,serum biomarkers of donors,such as lipids and electrolytes,have drawn increasing attention due to their effects on the postoperative outcomes of renal *** study aimed to examine the value of these serum biomarkers for prediction of renal graft *** The present study consecutively collected 306 patients who underwent their first single kidney transplantation(KT)from adult deceased donors in our center from January 1,2018 to December 31,*** correlation between postoperative outcomes[DGF and abnormal serum creatinine(SCr)after 6 and 12 months]and risk factors of donors,including gender,age,body mass index(BMI),past histories,serum lipid biomarkers[cholesterol,triglyceride,high-density lipoprotein(HDL)and low-density lipoprotein(DL)],and serum electrolytes(calcium and sodium)were analyzed and ***(1)Donor age and pre-existing hypertension were significantly correlated with the incidence rate of DGF and high SCr level(≥2 mg/dL)at 6 and 12 months after KT(Plipids,merely the low level of serum HDL of the donor was correlated with the reduced incidence rate of high SCr level at 12 months after KT[P<0.05,OR(95%CI):0.425(0.202–0.97)];(4)The serum calcium of the donor was associated with the reduced incidence rate of high SCr level at 6 and 12 months after KT[P<0.05,OR(95%CI):0.184(0.045–0.747)and P<0.05,OR(95%CI):0.114(0.014–0.948),respectively].Conclusion The serum HDL and calcium of the donor may serve as predictive factors for the postoperative outcomes of renal grafts after KT,in addition to the donor’s age,BMI and pre-existing hypertension.

关键词： delayed graft function serum creatinine serum high-density lipoprotein serum calcium kidney transplantation

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第五届全国生物信息学与系统生物学学术大会暨国际生物信息学前沿研讨会高通量时代的生物信息学与系统生物学

作者： xiao-qin li Hai-Song Xu Bioengineering Center Beijing University of Technology

Proteins play an important role in structural constitution and functional activity of *** all these take proteins’ forming certain spatial conformation as a premise. Amino acid sequences of proteins generated from evolution should contain the information for the assumption of the native secondary and tertiary ***,the functional role of proteins obliges it to keep thermostable and this thermostable information is encoded by protein sequences all the *** a matter of fact,the fold-ability and the thermostability are both the characteristic properties of protein *** these characteristic properties would contribute to protein structure predicting and new protein sequence designing. We study the folding related information in protein sequences by constructing aminoacid scoring matrix and modified statistical coupling analysis method(SCA).Herein,three protein structure class-specific score matrices(ALPHASUM,BETASUM and AFBETASUM) were constructed based on the structure alignment of low identity(lignment of SH3 domain was analyzed using the method of SCA. The class-specific score matrices were significantly better than a structure-derived matrix (HSDM) and three other generalized matrices(BLOSUM30,BLOSUM60 and Gonnet250) in alignment performance *** optimized gap penalties also promote alignment *** statistical conserve energy from SCA method could evaluate the site conservation of SH3 sequence set *** sites perturbing analysis revealed local and nonlocal perturbing modes in SH3 *** perturbing modes which involved with different perturbing sites exist in SH3 *** sharing common perturbing sites and responding sites,different perturbing modes could *** the coupling responding mode of all sites in the structure was *** sites with high average coupling energy correspond

关键词： Protein folding protein thermostability score matrix amino acid substitution pattern coupling analysis

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不确定奇异时滞系统的时滞相关型鲁棒H∞弹性控制

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控制理论与应用 2008年第4期25卷 665-670,676页

作者：高焕丽胥布工秦小丽华南理工大学自动化科学与工程学院广东广州510640 北京理工大学宇航科学技术学院北京100081

讨论了不确定奇异时滞系统的时滞相关型鲁棒弹性控制器设计问题.在一个关于标称奇异时滞系统的新的时滞相关型稳定性判据的基础上给出了一种新的有界实引理(BRL:bounded real lemma);基于此给出了时滞相关型鲁棒弹性控制器存在的充分性... 详细信息

讨论了不确定奇异时滞系统的时滞相关型鲁棒弹性控制器设计问题.在一个关于标称奇异时滞系统的新的时滞相关型稳定性判据的基础上给出了一种新的有界实引理(BRL:bounded real lemma);基于此给出了时滞相关型鲁棒弹性控制器存在的充分性条件,最后通过数值算例说明本文结果的有效性.

关键词：鲁棒弹性控制奇异系统时滞相关型线性矩阵不等式(LMIs)

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Quantitative assessment of hematopoietic chimerism by quantitative real-time polymerase chain reaction of sequence polymorphism systems after hematopoietic stem cell transplantation

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Chinese Medical Journal 2011年第15期124卷 2301-2308页

作者： qin xiao-ying li Guo-xuan qin Ya-zhen WANG Yu WANG Feng-rong liU Dai-hongx liU Dai-hong XU Lan-ping CHEN Huan HAN Wei WANG Jing-zhi ZHANG xiao-hui li Jin-lan li ling-di liU Kai-yan HUANG xiao-jun Peking University People＇s Hospital Peking University Institute of Hematology Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation Beijing 100044 China

Background Analysis of changes in recipient and donor hematopoietic cell origin is extremely useful to monitor the effect of hematopoietic stem cell transplantation （HSCT） and sequential adoptive immunotherapy by donor lymphocyte infusions. We developed a sensitive, reliable and rapid real-time PCR method based on sequence polymorphism systems to quantitatively assess the hematopoietic chimerism after HSCT. Methods A panel of 29 selected sequence polymorphism （SP） markers was screened by real-time PCR in 101 HSCT patients with leukemia and other hematological diseases. The chimerism kinetics of bone marrow samples of 8 HSCT patients in remission and relapse situations were followed longitudinally. Results Recipient genotype discrimination was possible in 97.0% （98 of 101） with a mean number of 2.5 （1-7） informative markers per recipient/donor pair. Using serial dilutions of plasmids containing specific SP markers, the linear correlation （r） of 0.99, the slope between -3.2 and -3.7 and the sensitivity of 0.1% were proved reproducible. By this method, it was possible to very accurately detect autologous signals in the range from 0.1% to 30%. The accuracy of the method in the very important range of autologous signals below 5% was extraordinarily high （standard deviation 〈1.85%） which might significantly improve detection accuracy of changes in autologous signals early in the post-transplantation course of follow-up. The main advantage of the real-time PCR method over short tandem repeat PCR chimerism assays is the absence of PCR competition and plateau biases, with demonstrated greater sensitivity and linearity. Finally, we prospectively analyzed bone marrow samples of 8 patients who received allografts and presented the chimerism kinetics of remission and relapse situations that illustrated the sensitivity level and the promising clinical application of this method. Conclusion This SP-based real-time PCR assay provides a rapid, sensitive, and accurate quantitative as

关键词： sequence polymorphism single nucleotide polymorphism real-time PCR hematopoietic stem cell transplantation chimerism

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功能性脂质纳米分散体研究进展

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现代食品科技 2014年第12期30卷 294-301页

作者：钟金锋覃小丽西南大学食品科学学院重庆400715

功能性脂质(类胡萝卜素、植物甾醇、脂溶性维生素、n-3多不饱和脂肪酸等)具有丰富营养价值和多种生理功能,但其自身存在水溶性差,食品基质中功能性脂质在消化系统中溶解度低、肠胃吸收率低和生物利用度低等缺点,限制了其在食品和医药领... 详细信息

功能性脂质(类胡萝卜素、植物甾醇、脂溶性维生素、n-3多不饱和脂肪酸等)具有丰富营养价值和多种生理功能,但其自身存在水溶性差,食品基质中功能性脂质在消化系统中溶解度低、肠胃吸收率低和生物利用度低等缺点,限制了其在食品和医药领域的广泛应用。改善功能性脂质的水溶性、贮存稳定性及提高其生物利用度,已成为普遍关注和急需要解决的问题。利用纳米技术制备功能性脂质纳米分散体有望能增加功能性脂质的溶解度和生物利用度,从而扩大功能性脂质在食品等行业的应用范围。本文在国内外文献分析的基础上,综述了功能性脂质纳米分散体制备所需材料和制备方法,并分析了纳米分散体系中功能性脂质理化稳定性的影响因素和初步阐述了功能性脂质纳米分散体的生物利用度和安全性。最后,对目前功能性脂质纳米分散体研究中的科学问题和重点进行了总结和展望。

关键词：功能性脂质纳米分散体制备方法稳定性吸收

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Relationship between pre-TIPS hepatic hemodynamics and postoperative incidence of hepatic encephalopathy

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Hepatobiliary & Pancreatic Diseases International 2006年第2期5卷 232-236页

作者： Dan Deng, Ming-Song liao, Jian-Ping qin and xiao-An li Departments of Ultrasonography Gastroenterology General Hospital, Cheng- du Military Command, Chengdu 610083, China Department of Ultrasonography General Hospital Chengdu Military Command Chengdu 610083 China. dengdan_888@***

BACKGROUND: Hepatic encephalopathy (HE) is one of the complications that have limited the effectiveness of transjugular intrahepatic portosystemic shunt (TIPS) most significantly. Up to the present, the predicting factors of HE post-TIPS have been debated controversially. This study was undertaken to verify the relationship between pre-TIPS intrahepatic hemodynamics and the incidence of post-TIPS HE. METHODS: The hepatic blood dynamics was evaluated in 41 patients with liver cirrhosis before TIPS and at one month after TIPS by ultrasonography. The patients were divided into two groups according to Doppler findings before TIPS: group 1, patients with prograde portal flow, and group 2, patients with hepatofugal or back-forth portal flow. The clinical characteristics (age, sex, etiology of liver disease, pre-TIPS Child-Pugh score, incidence of pre-TIPS HE, and portacaval pressure gradient), incidence of post TIPS HE, and pre-/post-TIPS hepatic arterial resistant index (RI) in the two groups were compared. The independent prognostic value of pre-TIPS variables for the onset of HE after TIPS, including age, Child-Pugh score, presence of HE before TIPS, and the pattern of portal flow, was tested with a multiple-factor regression analysis. RESULTS: No significant difference in age, etiology of liver disease, indications of TIPS placement, incidence of HE before TIPS, and portacaval gradient before and after TIPS was observed between the two groups; but liver failure was more severe in group 2 (P<0.05). The incidence of post-TIPS HE in group 2 was significantly lower than that in group 1 (P<0.01). Pre-TIPS, the RI of the hepatic artery in group 1 was significantly higher than that in group 2 (P<0.01). However, TIPS induced a significantly decreased RI in group 1 (P <0.01), but not in group 2. Multiple-factor regression analysis demonstrated that the pattern of portal flow before TIPS was closely associated with the onset of post TIPS HE. CONCLUSIONS: Pre-TIPS intrahepatic h

关键词： ultrasonography liver transjugular intrahepatic portosystemic shunt hepatic encephalopathy hepatic hetnodynamics

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公共供求理论视域下残疾人体育公共服务体系研究——以浙江省为例

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体育科学 2012年第3期32卷 17-27页

作者：肖丽琴杭州电子科技大学信息工程学院浙江杭州310018

为全面了解残疾人在开展体育健身活动时享有的基本体育公共服务情况,基于公共供求关系理论对浙江省残疾人体育公共服务体系的基本现状,包括残疾人体育公共服务体系的需求及供给特征进行了调查分析,并探索了残疾人体育公共服务体系发展... 详细信息

为全面了解残疾人在开展体育健身活动时享有的基本体育公共服务情况,基于公共供求关系理论对浙江省残疾人体育公共服务体系的基本现状,包括残疾人体育公共服务体系的需求及供给特征进行了调查分析,并探索了残疾人体育公共服务体系发展的影响路径。结果表明:浙江省残疾人体育人口比例较低(调查对象:16岁以上视残、听残、肢残三类残疾人),仅8.9%,但参与体育活动的愿望迫切,有80%以上的残疾人希望政府能为其提供适合的体育公共服务体系;残疾人体育公共服务体系需求合成了不同的因子成分;不同性别、城乡和残疾类型的残疾人体育公共资源需求满意度呈现了较低的认可。另外,浙江省残疾人体育公共服务体系的供需失衡,残疾人体育公共服务体系平台尚未建立,体育公共服务和公共产品供给数量严重不足,且结构不合理,投入经费不足;特殊体育指导员匮乏;供给制度不完善;信息闭塞;供给主体不明确,供给意愿不强烈。针对调查中发现的问题,提出相应建议。

关键词：浙江残疾人体育公共服务体系公共供求理论供需特征发展举措

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浙江省特殊教育学校体育现状及影响因素研究

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中国体育科技 2009年第5期45卷 99-106页

作者：肖丽琴杭州电子科技大学信息工程学院浙江杭州310018

为推动学校体育改革的深入发展,进一步加强特殊教育学校体育工作,运用问卷调查、座谈访问、文献资料调研、数理统计等研究方法,对浙江省特殊教育学校体育现状进行了调查分析,包括特教学校体育管理、体育经费、体育师资、体育教学、体育... 详细信息

为推动学校体育改革的深入发展,进一步加强特殊教育学校体育工作,运用问卷调查、座谈访问、文献资料调研、数理统计等研究方法,对浙江省特殊教育学校体育现状进行了调查分析,包括特教学校体育管理、体育经费、体育师资、体育教学、体育场(馆)器材、学生的体育认知等。并通过R型因子分析,找出了影响特教学校体育发展的5个因子,权重分别为:领导与经费因子(30.08%),教师与教学因子(18.201%),运动场(馆)因子(10.329%),安全因子(7.00%),学生对体育的认知因子(5.726%)。针对调查中出现的问题,提出了相应的发展建议。

关键词：特殊教育学校体育现状影响因素浙江

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