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Chlorogenic acid alleviates hypoxic-ischemic brain injury in neonatal mice

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Neural Regeneration Research 2023年第3期18卷 568-576页

作者： Lu-Yao Li Qi Wang Lu Deng Zhen Lin Jing-Jing Lin Xin-Ye Wang Tian-Yang Shen Yi-Hui Zheng Wei Lin Pei-Jun Li xiao-qin fu Zhen-Lang Lin Department of Pharmacy The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Neonatology The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Geriatrics The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Orthopedics The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina Department of Neurology The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Recent studies have shown that chlorogenic acid(CGA),which is present in coffee,has protective effects on the nervous ***,its role in neonatal hypoxic-ischemic brain injury remains *** this study,we established a newborn mouse model of hypoxic-ischemic brain injury using a modified Rice-Vannucci method and performed intraperitoneal injection of *** found that CGA intervention effectively reduced the volume of cerebral infarct,alleviated cerebral edema,restored brain tissue structure after injury,and promoted axon growth in injured brain ***,CGA pretreatment alleviated oxygen-glucose deprivation damage of primary neurons and promoted neuron *** addition,changes in ferroptosis-related proteins caused by hypoxic-ischemic brain injury were partially reversed by ***,CGA intervention upregulated the expression of the key ferroptosis factor glutathione peroxidase 4 and its upstream glutamate/cystine antiporter related factors SLC7A11 and *** summary,our findings reveal that CGA alleviates hypoxic-ischemic brain injury in neonatal mice by reducing ferroptosis,providing new ideas for the treatment of neonatal hypoxic-ischemic brain injury.

关键词： chlorogenic acid ferroptosis glutathione peroxidase 4 lipid peroxidation neonatal hypoxic-ischemic brain injury neurons neuroprotection oxidative stress oxygen-glucose deprivation system Xc^(-)

Ultrafiltration of pumpkin polysaccharide enhanced by ultrasonic and electric field

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Ultrafiltration of pumpkin polysaccharide enhanced by ultras...

广东省生物物理学会2013年学术研讨会

作者： Di Zhao Bing Li xiao-qin fu College of Light Industry and Food Sciences South China University of Technology

Ultrasonic and electric field were introduced into pumpkin polysaccharide ultrafiltration independently or *** of ultrasonic including ultrasonic power,frequency as well as temperature and pressure during ultrafiltration were investigated by inspecting their effects on permeation flux or rejection rate Then,effects of electric field including electric intensity and pulse frequency were investigated in the same *** last,effects of complex fields were *** results indicated that:(l)both the introduction ultrasonic and electric field could enhance ultrafiltration efficiency;(2)There were peak values of the enhancement of electric field,when electric intensity exceeded 625V/cm or pulse frequency exceeded 600Hz,ultrafiltration efficiency decreased;(3)A cooperation effect was observed when the complex field was introduced;(4)introducing physical fields contributed to reduction of ultrafiltration membrane contamination and recovery of permeation flux with following sequence: 8kHz ultrasonic +625V/cm electric field >40kHz ultrasonic +625V/cm electric field >625V/cm electric field >28kHz ultrasonic >650V/cm electric field >40kHz ultrasonic.

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蛋白质结构型的识别方法

生物化学与生物物理进展 2002年第6期29卷 938-941页

作者：李晓琴罗辽复内蒙古大学物理系呼和浩特010021

给出了α型、β型、α/ β型、多域型蛋白质二级结构主序列六联体的分布规律 .提出了根据蛋白质二级结构主序列对蛋白质结构型进行识别 (分类 )的方法 .以蛋白质二级结构主序列三联体为参数 ,利用Mahalanobis距离方法对上述 4种结构型... 详细信息

给出了α型、β型、α/ β型、多域型蛋白质二级结构主序列六联体的分布规律 .提出了根据蛋白质二级结构主序列对蛋白质结构型进行识别 (分类 )的方法 .以蛋白质二级结构主序列三联体为参数 ,利用Mahalanobis距离方法对上述 4种结构型的蛋白质进行识别 ,分类的总体准确率为 81% ;以二级结构主序列中六联体的频数构成蛋白质结构的多样性源 ,利用多样性增量极小化对上述 4种结构型进行识别 ,分类的总体准确率为 83% .

关键词：蛋白质结构型识别方法二级结构序列多样性指标

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蛋白质结构型的定义和识别

生物化学与生物物理进展 2002年第1期29卷 124-127页

作者：李晓琴罗辽复内蒙古大学理工学院呼和浩特010021

提出紧结构域的概念 ,由二级结构序列中一段或几段连续的α螺旋和 β折叠构成的空间紧密堆集的最大折叠体称为紧结构域 .利用 3种紧结构域 (α域 ,β域和α/β域 )定义球蛋白的 5种结构型 :α型蛋白 ,β型蛋白 ,α/β型蛋白 ,多域蛋白和... 详细信息

提出紧结构域的概念 ,由二级结构序列中一段或几段连续的α螺旋和 β折叠构成的空间紧密堆集的最大折叠体称为紧结构域 .利用 3种紧结构域 (α域 ,β域和α/β域 )定义球蛋白的 5种结构型 :α型蛋白 ,β型蛋白 ,α/β型蛋白 ,多域蛋白和 ζ型蛋白 .将 12 6 1个代表性的蛋白质 (10 2 2家族 )进行分类 ,并和SCOP库的分类做了比较 .进行了删去序列冗余的分析 .在此基础上提出结构型的预测方案 ,成功率在 82 %～ 85 %.

关键词：蛋白质结构型二级结构序列紧结构域序列冗余预测识别

Gene and protein expressions of p28^(GANK)in rat with liver regeneration

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World Journal of Gastroenterology 2003年第11期9卷 2523-2527页

作者： Jian-Min qin xiao-Yong fu Shen-Jing Li Shu-qin Liu Jin-Zhang Zeng Xiu-Hua Qiu Meng-Chao Wu Hong-Yang Wang, International Co-operation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute, Second Military Medical University, Shanghai, 200438, China International Co-operation Laboratory on Signal Transduction Eastern Hepatobiliary Surgery Institute Second Military Medical University Shanghai 200438 China.

AIM: To observe the gene and protein expression changes of p28GANK in regenerating liver tissues, and to reveal the biological function of p28GANK on the regulation of liver ***: One hundred and thirty two adult male Sprague-Dawley rats were selected, weighing 200-250 g,and divided randomly into sham operation (SO) group and partial hepatectomy (PH) group. Each group had eleven time points: 0, 2, 6, 12, 24, 30, 48, 72, 120, 168 and 240 h,six rats were in each time point. The rats were undergone 70 % PH under methoxyflurane anesthesia by resection of the anterior and left lateral lobes of the liver. SO was conducted by laparotomy plus slight mobilization of the liver without resection. Liver specimens were collected at the indicated time points after PH or SO. The expression level of p28GANK mRNA was determined by Northern blot as well as at protein level via immunohistochemical *** expressions of p28GANK mRNA in these tissues were analyzed by imaging analysis system of FLA-2000 fuJIFILM and one way analysis of variance. The protein expressions of p28GANK in these tissues were analyzed with Fromowitz'method and Rank sum ***: The expression of p28GANK mRNA in bhe regenerating liver tissues possessed two transcripts, which were 1.5 kb and 1.0 kb. There was a significantly different expression patterns of p28GANK mRNA between SO and PH groups (P<0.01). The expression of p28GANK mRNA increased 2 h after PH, the peak time was 72 h (SO group: 163.83±1.4720; PH group: 510.5±17.0499, P<0.01). There was a significant difference in the 1.5 kb transcript, which decreased gradually after 72 hours. The protein expression of p28GANK was mainly in the cytoplasm of regenerating hepatocytes, and increased near the central region 24 h after PH, and became strongly positive at 48 h (+++, vs the other time points P<0.05),but decreased 72 h after ***: The expression of p28GANK mRNA increases in the early stage of rat liver regeneration, the pr

关键词： p28GANK 肝组织重建基因表达动物实验细胞增殖作用机制

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漂浮式光伏电站漂浮方阵流载荷数值计算研究

水动力学研究与进展（A辑） 2019年第2期34卷 218-223页

作者：孔耀华肖福勤陈作钢宋肖锋吴昊上海交通大学海洋工程国家重点实验室上海200240 阳光电源股份有限公司安徽合肥230088 淮南阳光浮体科技有限公司安徽淮南232089 高新船舶与深海开发装备协同创新中心(船海协创中心) 上海200240

漂浮式光伏电站具有组件多和规模大的特点,对其流载荷的评估尚未有成熟方法。该文首先以风洞试验对缩尺的部分模型方阵风载荷的CFD计算进行精度验证,然后对170 m×170 m三维漂浮方阵在不同来流角度下所受流载荷进行了计算和分析,获得了... 详细信息

漂浮式光伏电站具有组件多和规模大的特点,对其流载荷的评估尚未有成熟方法。该文首先以风洞试验对缩尺的部分模型方阵风载荷的CFD计算进行精度验证,然后对170 m×170 m三维漂浮方阵在不同来流角度下所受流载荷进行了计算和分析,获得了流载荷的分布规律。结合典型工况下流载荷CFD的3D计算结果,验证了该文提出的2.5D方法模拟3D计算的合理性。大型漂浮方阵的流载荷研究可先计算2.5D流载荷,再通过3D计算得到的载荷分布规律推算出整个方阵的流载荷。在0.5 m/s流速以上,无量纲的流载荷系数与流速基本无关。

关键词：漂浮式光伏电站漂浮方阵流载荷数值模拟 2.5D计算

BIBR1532 inhibits proliferation and metastasis of esophageal squamous cancer cells by inducing telomere dysregulation

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 214-225页

作者： qin Wang qing-Rong Li Lei Xu Zi-Chun Yuan xiao Liu Mao-Ju Tang Man Luo xiao-Wu Zhong Qiang Ma xiao-Lan Guo Department of Clinical Laboratory Affiliated Hospital of North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina School of Laboratory Medicine&Translational Medicine Research Center North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina

BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a malignant tumor with high morbidity and mortality,and easy to develop resistance to chemotherapeutic *** are DNA-protein complexes located at the termini of chro-mosomes in eukaryotic cells,which are unreplaceable in maintaining the stability and integrity of ***,an RNA-dependent DNA polymerase,play vital role in telomere length maintain,targeting telomerase is a promising therapeutic strategy for ***150 and KYSE410 cells were cultured and exposed to various concentrations of *** viability was assessed at 48 hours and 72 hours to determine the IC50 *** effects of BIBR1532 on ESCC cell proliferation,migration,and cellular senescence were evaluated using the cell counting kit-8 assay,plate colony formation assay,scratch assay,transwell assay,andβ-galactosidase staining,*** blotting was performed to detect the expression of RESULTS The IC50 values for KYSE150 and KYSE410 cells after 48 hours of BIBR1532 exposure were 48.53μM and 39.59μM,*** values decreased to 37.22μM and 22.71μM,respectively,following a longer exposure of 72 ***1532 exhibited dose-dependent effects on KYSE150 and KYSE410 cells,including decreased hTERT expression,inhibition of proliferation and metastasis,and induction of cellular ***,BIBR1532 upregulated the expression of the DDR protein,γ-H2AX,and activated the ataxia telangiectasia and Rad3-related protein(ATR)/check point kinase 1(CHK-1)and ataxia-telangiectasia mutated gene(ATM)/CHK2 ***1532 downregulated the expression of telomere-binding proteins,including telomeric-repeat binding factor 1(TRF1),TRF2,protection of telomeres 1,and TIN2-interacting protein *** a nude mouse xenograft model,BIBR1532 significantly suppressed tumor growth,reduced hTERT expression,and increasedγ-H2AX protein *** and eosin staining of various organs,including the heart,liver,s

关键词： Esophageal squamous cell carcinoma BIBR1532 Human telomerase reverse transcriptase DNA damage response Telomere-binding proteins

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漂浮式光伏电站方阵环境载荷计算方法研究

工程力学 2020年第3期37卷 245-256页

作者：肖福勤陈作钢代燚宋肖锋郭军余德海吴昊淮南阳光浮体科技有限公司安徽淮南232000 阳光电源股份有限公司安徽合肥230088 上海交通大学海洋工程国家重点实验室上海200240 上海交通大学船舶海洋与建筑工程学院上海200240 高新船舶与深海开发装备协同创新中心(船海协创中心) 上海200240

针对我国建成的全球首个单体容量达到40 MW的漂浮式光伏电站方阵进行了环境载荷数值预报研究,在与风洞模型试验结果对比验证的基础上,基于计算流体力学(computational fluid dynamics,CFD)方法对该大规模方阵的整体风载荷和流载荷进行... 详细信息

针对我国建成的全球首个单体容量达到40 MW的漂浮式光伏电站方阵进行了环境载荷数值预报研究,在与风洞模型试验结果对比验证的基础上,基于计算流体力学(computational fluid dynamics,CFD)方法对该大规模方阵的整体风载荷和流载荷进行了数值研究。CFD计算的数值考察验证了采用简化浮体模型进行研究的可行性,单体模型的计算结果与风洞试验结果吻合良好;对方阵进行实尺度建模及3D简略计算,得到了整体风载荷在不同风向角下的变化规律;通过对最大载荷风向的北风条件下的载荷分布规律的分析,提出了2.5D计算的策略,得到了数值验证;根据计算结果设计并实施了18行′11列单元模块的缩尺方阵风洞试验,试验结果和CFD计算结果吻合良好,据此构建了完整的数值计算方法;对2.5D进行高精度计算,确定了针对3D简略计算的修正系数,得到较为准确的整体风载荷结果。流载荷计算方法与风载荷相同。采取势流计算方法开展了波浪载荷数值分析研究,得到了在单位波幅规则波作用下漂浮方阵波浪载荷随行和列的变化规律;根据此规律获得了整体漂浮方阵的波浪载荷,并据此计算了50年一遇的极端条件下漂浮方阵所受的波浪载荷。该研究为漂浮式光伏电站的风、流、波浪等环境载荷的数值预报提供了方法,为漂浮方阵的锚泊计算及水上光伏电站的系统设计提供了技术支持。

关键词：漂浮式光伏电站环境载荷 CFD数值模拟 2.5D计算风洞试验

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang Yanlin fu Peiyan Long xiao Gao Zhengwei Wang Zhizhong Guan xiaolan Qi Wei Hong Yan xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK