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In vivo suppressive effect of nudear factor-kB inhibitor on neutrophilic inflammation of grafts after orthotopic liver transplantation in rats

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World Journal of Gastroenterology 2004年第24期10卷 3654-3658页

作者： xiao-pinggu Yu-DongQiu Fu-TaoXu YongJiang Yi-TaoDing DepartmentofAnesthesiology DrumTowerHospitalMedicalDepartmentofNanjingUniversityNanjing210008JiangsuProvinceChina DepartmentofHepatobiliarySurgery DrumTowerHospitalMedicalDepartmentofNanjingUniversityNanjing210008JiangsuProvinceChina

AIM. To investigate the effect of pyrrolidine dithiocarbamate (PDTC), a novel nuclear factor-κB (NF-κB) inhibitor, on expression of multiple inflammatory mediators and neutrophilic inflammation of cold preserved grafts after rat liver transplantation and its ***: Orthotopic liver transplantation (OLT) was performed after 24 h of cold storage using University of Wisconsin solution with varied concentrations of PDTC. We determined the time course of NF-κB activation and expression of multiple inflammatory signals, such as tumor necrosis factor-α (TNF-α), cytokine-inducible neutrophil chemoattractant (CINC), and intercellular adhesion molecule-1(ICAM-1) by ELISA methods. Serum alanine aminotransferase (ALT), intrahepatic myeloperoxidase (MPO)/WBC (a measure of neutrophil accumulation) and Mac-1 expression (a measure of circulating neutrophil activity) were also ***: PDTC decreased NF-κB activation induced by prolonged cold preservation in a dose dependent manner (from 20 mmol/L to 60 mmol/L), diminished TNF-α, CINC,ICAM-1 proteins in the grafts, and reduced the expression f increases in plasma TNF-α levels induced by prolonged old preservation. Neutrophilic inflammation of the graft was significantly suppressed after preservation with PDTC (P<0.05). The total neutrophil accumulation in PDTC (40 mmol/L) group (7.04±0.97) was markedly reduced compared to control group (14.07±1.31) (P<0.05). Mac-1 expression was significantly reduced in PDTC (40 retool/L) group (181±11.3%) compared with the control group (281±13.2%) (P<0.05) at 6 h after reperfusion. Furthermore,PDTC inhibited the increased serum ALT levels after liver ***: PDTC can inhibit B NF-κB activation and expression of the inflammatory mediators, which are associated with improved graft viability via inhibiting intrahepatic neutrophilic inflammation. Our study suggests that a therapeutic strategy directed at inhibition of NF-κB activation in the transplanted li

关键词：抑制作用因子-κB 抑制剂嗜中性炎症器官移植常位肝脏移植老鼠消化系统

Effect of cold-ischemia time on nuclear factor-кB activation and inflammatory response in graft after orthotopic liver transplantation in rats

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World Journal of Gastroenterology 2004年第7期10卷 1000-1004页

作者： xiao-pinggu YongJiang Fu-TaoXu Yu-DongQiu Yi-TaoDing DepartmentofAnesthesiology GulouHospitalNanjingUniversityMedicalCenterNanjing210008JiangsuProvinceChina DepartmentofHepatobiliarySurgery GulouHospitalNanjingUniversityMedicalCenterNanjing210008JiangsuProvinceChina

AIM: To study the mechanism and effect of nuclear factor-κB (NF-κB) activation and inflammatory response on the extended cold-preserved graft injury after orthotopic liver transplantation (OLT). METHODS: OLT was performed in rats with varying time of cold ischemia grafts (6, 18 and 24 h in University Wisconsin solution at 4 ℃). We determined the time of NF-κB activation and expression of tumor necrosis factor-α (TNF-α), cytokineinducible neutrophil chemoattractant (CINC), and intercellular adhesion molecule-1 (ICAM-1) within 6 h after reperfusion. Serum alarming aminotransferase (ALT), neutrophil sequestration, circulating neutrophil CD11b and L- selectin expression were also evaluated. RESULTS: The accumulation of neutrophils in the graft was significantly increased in the 18 h and 24 h cold-ischemia groups within 0.5 h after reperfusion, compared with the 6 h group. But the strongly activated neutrophils was slightly increased at 2 h after reperfusion and remained at high levels 4 h after reperfusion, which was synchronized with the common situation of recipients after transplantation. Prolonged cold-preservation did not affect neutrophil accumulation and activation. NF-κB activation preceded the expression of TNF-α, CINC, and ICAM-1 in the liver, which was significantly increased with prolonged cold preservation. In prolonged cold preserved grafts, prominently elevated NF-κB activation occurred at 0.5 h and 1 h, compared with that at 2 h after reperfusion, which was consistent with greatly increased intrahepatic TNF-α response. CONCLUSION: NF-κB activation is correlated with the expression of TNF-α, CINC, and ICAM-1 in vivo in OLT rats. Extended cold preservation of grafts might up-regulate TNF-α, CINC, and ICAM-1 expression in the grafts, most probably through elevated NF-κB activation, and might contribute to neutrophil infiltration in the grafts after reperfusion. Elevated NF-κB activity is harmful to inflammatory response in the grafts, and inhibited NF-κB ac

关键词：常位肝移植动物实验肝缺血冷冻保存炎症反应神经因子-κB

Ischemic preconditioning decreases C-X-C chemokine expression and neutrophil accumulation early after liver transplantation in rats

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World Journal of Gastroenterology 2003年第9期9卷 2025-2029页

作者： YongJiang xiao-pinggu Yu-DongQiu Xue-MeiSun Lei-LeiChen Li-HuaZhang Yi-TaoDing DepartmentofHepatobiliarySurgery GulouHospitalMedicalD~partmentofNanjingUniversityNanjing210008JiangsuProvinceChina DepartmentofBiochemicalAssay GulouHospitalMedicalDepartmentofNanjingUniversityNanjing210008JiangsuProvinceChina DepartmentofPathology GulouHospitalMedicalDepartmentofNanjingUniversityNanjing210008.JiangsuProvinceChina

AIM: Polymorphonuclear neutrophil (PMN) plays a major role in liver ischemia/reperfusion injury. Protective effect of ischemic preconditioning (IP) has been confirmed in liver ischemia/reperfusion injury. The purpose of this study was to investigate the effect of IP on C-X-C chemokine expression and PMNs recruitment eady after liver ***: Male Sprague-Dawley rats were used as donors and recipients of orthotopic liver transplantation (OLT). The donor liver was stored 24 hours in University of Wisconsin(UW) solution at 4 ℃ pre-implantation, IP was done by damp of the portal vein and hepatic artery of the donor liver for 10 minutes followed by reperfusion for 10 minutes before harvesting. The neutrophilic infiltration in liver was quantified using a myeloperoxidase (MPO) assay. Intragrafl: expression of macrophage inflammatory protein-2 (MIP-2) mRNA was investigated with in situ hybridization, The serum levels of MIP-2 and tumor necrosis factor (TNF)-α were also monitored,RESULTS: After liver transplantation without IP, the hepatic MPO increased significantly compared with sham operated group. In IP group, PMN in liver indicated by MPO was reduced significantly. In situ hybridization showed no MIP-2 mRNA in sham group but dramatic expression in hepatocytes in non-IP group. In IP group, MIP-2 mRNA was significantly down-regulated. Similarly, serum MIP-2 and TNF-α levels were significantly elevated in non-IP group and both were reduced in IP ***: IP might protect graft liver from preservation-reperfusion injury after OLT through down-regulating C-X-C chemokine expression of hepatocytes, and alleviating PMNs recruitment after reperfusion.

关键词：多形核中性白细胞肝移植缺血再灌注损伤缺血预处理

我国进口干酪制品中硝酸盐、亚硝酸盐含量调查及暴露风险评估研究

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中国食品卫生杂志 2022年第3期34卷 415-420页

作者：李影周萍萍张磊陈潇吴平谷王军淋曹佩安徽省疾病预防控制中心食品营养与学校卫生科安徽合肥230601 国家食品安全风险评估中心北京100022 浙江省疾病预防控制中心浙江杭州310051

目的了解我国进口干酪制品中硝酸盐、亚硝酸盐含量水平,并评估我国成人干酪消费人群健康风险。方法基于我国市售的进口干酪制品中硝酸盐、亚硝酸盐含量和2012年我国居民干酪消费量数据,通过概率评估方法,对我国成人干酪消费人群的膳食... 详细信息

目的了解我国进口干酪制品中硝酸盐、亚硝酸盐含量水平,并评估我国成人干酪消费人群健康风险。方法基于我国市售的进口干酪制品中硝酸盐、亚硝酸盐含量和2012年我国居民干酪消费量数据,通过概率评估方法,对我国成人干酪消费人群的膳食硝酸盐、亚硝酸盐暴露量进行估计,并分别与联合国粮农组织/世界卫生组织食品添加剂联合专家委员会(JECFA)制定的硝酸盐每日允许摄入量(ADI)和欧洲食品安全局(EFSA)制定的亚硝酸盐ADI比较后进行风险表征。结果162份进口干酪样品中,硝酸盐的检出率为89.5%,平均含量为11.23 mg/kg;亚硝酸盐仅2份检出,含量为0.4和0.6 mg/kg。不同种类进口干酪制品中硝酸盐的平均含量从高到低依次为软质、半硬质、其他、硬质。我国成人通过进口干酪硝酸盐的平均暴露量为5.13μg/kg BW/d,占ADI的0.14%,高暴露人群(P95)为20.06μg/kg BW/d,占ADI的0.55%。结论我国进口干酪制品中硝酸盐的检出率较高,且不同种类进口干酪制品其含量存在差异,而亚硝酸盐检出率很低。我国成人通过进口干酪暴露硝酸盐的健康风险较低。

关键词：进口食品干酪硝酸盐亚硝酸盐风险评估

Effectiveness of Xinglouchengqi decoction on constipation in patients with acute ischemic stroke: a randomized controlled trial

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Journal of Traditional Chinese Medicine 2020年第1期40卷 112-120页

作者： Chen Pei Jiang Lan Geng Hualei Han xiao Xu Yinping Chen Yan Guo Jinbin Wu Shuang Wang Yahui Yan Ruyu Ren Yi ZouYihuai Department of Neurology Dongzhimen Hospital affiliated to Beijing University of Chinese MedicineBeijing 100700China The National Clinical Research Center for Mental Disorders&Beijing Key Laboratory of Mental Disorders Beijing Anding HospitalCapital Medical University&Advanced Innovation Center for Human Brain ProtectionCapital Medical UniversityBeijing 100088China Department of Neurology Beijing Pinggu Hospital of Traditional Chinese MedicineBeijing 101200China Department of Neurology Beijing Changping Hospital of Traditional Chinese MedicineBeijing 102200China Department of Neurology Hospital affiliated to Changzhi Academy of Chinese Medical SciencesChangzhi 046000China Department of Emergency Beijing University of Chinese Medicine Third Affiliated HospitalBeijing 100029China

OBJECTIVE:To evaluate the effectiveness of traditional Chinese herbal Xinglouchengqi(XLCQ)decoction for the treatment of constipation in acute ischemic stroke patients,and figure out the role that bowel movements play in the treatment of acute ischemic ***:A total of 317 eligible patients were recruited and randomized to the XLCQ group(211 patients)or the control group(106 patients).In addition to conventional standard medical care and rehabilitation,participants in the XLCQ group received XLCQ decoction,while the control group received clysis therapy using glycerin enemas or lactulose oral *** groups were given treatment for 3 to 6 d,during which they received daily visits to record defecation features and accompanying *** assessments using the National Institutes of Health Stroke Scale(NIHSS)were conducted before and 1 month after ***:Patients in the XLCQ group had lower aggregate constipation scores compared with the control group on days 3 and 5(P<0.05).Spontaneous bowel movements tended to reappear more rapidly after taking the XLCQ decoction than after conventional laxative *** the average aggregate constipation score and the time taken to achieve spontaneous bowel movements showed positive correlations with NIHSS scores before and1 month after treatment(P<0.01).CONCLUSION:Treatment with XLCQ decoction effectively alleviated the overall symptoms of constipation in acute ischemic stroke *** status of bowel movements in acute ischemic stroke can reflect the severity of neurological impairment and predict neurological outcomes at 1 month.

关键词： Stroke Constipation Xinglouchengqi decoction Randomized controlled trial

Novel glucose-lowering drugs for non-alcoholic fatty liver disease

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World Journal of Diabetes 2021年第1期12卷 84-97页

作者： Zuo-Di Fu xiao-Ling Cai Wen-Jia Yang Ming-Ming Zhao Ran Li Yu-Feng Li Department of Endocrinology Beijing Friendship Hospital Pinggu CampusBeijing 101200China Department of Endocrinology and Metabolism Peking University People’s HospitalBeijing 100044China The Institute of Cardiovascular Sciences School of Basic Medical SciencesHealth Science CenterPeking UniversityBeijing 100079China Sport Science School Beijing Sport UniversityBeijing 100078China Department of Endocrinology Beijing Friendship Hospital Pinggu CampusCapital Medical UniversityBeijing 101200China

BACKGROUND The efficacy of novel glucose-lowering drugs in treating non-alcoholic fatty liver disease(NAFLD)is *** To evaluate the efficacy of glucose-lowering drugs dipeptidyl peptidase-4(DPP-4)inhibitors,glucagon-like peptide-1 receptor agonists(GLP-1 RAs),and sodiumglucose cotransporter 2(SGLT2)inhibitors in treating NAFLD and to perform a comparison between these *** Electronic databases were systematically *** inclusion criteria were:Randomized controlled trials comparing DPP-4 inhibitors,GLP-1 RAs,or SGLT2 inhibitors against placebo or other active glucose-lowering drugs in NAFLD patients,with outcomes of changes in liver enzyme[alanine aminotransferase(ALT)and/or aspartate aminotransferase(AST)]from *** Nineteen studies were finally included in this *** with placebo or other active glucose-lowering drug treatment,treatment with DPP-4 inhibitors,GLP-1 RAs,and SGLT2 inhibitors all led to a significant decrease in ALT change and AST change from *** difference between the DPP-4 inhibitor and SGLT2 inhibitor groups in ALT change was significant in favor of DPP-4 inhibitor treatment(P<0.05).The trends of reduction in magnetic resonance imaging proton density fat fraction and visceral fat area changes were also observed in all the novel glucose-lowering agent treatment *** Treatment with DPP-4 inhibitors,GLP-1 RAs,and SGLT2 inhibitors resulted in improvements in serum ALT and AST levels and body fat composition,indicating a beneficial effect in improving liver injury and reducing liver fat in NAFLD patients.

关键词： Non-alcoholic fatty liver disease Glucose-lowering drug Meta-analysis Dipeptidyl peptidase-4 inhibitor Sodium-glucose cotransporter 2 inhibitor Glucagon-like peptide-1 receptor agonist

Analysis on the Spatio-temporal Patterns of Water Conservation Services in Beijing

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Journal of Resources and Ecology 2019年第4期10卷 362-372页

作者： XU Jie xiao Yu XIE Gaodi Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China School of Conservation Beijing Forestry University Beijing 100083 China

The shortage of water resources is a key factor limiting the sustainable development of the economy and society in Beijing. This study analysed the spatiotemporal patterns of Beijing’s water conservation services(WCS) based on the water balance equation at multiple scales, including city, main functional areas and key districts and counties, determined the differences in the water conservation amount among different land cover types and investigated the reasons for the spatiotemporal differences in the water conservation amount. The results indicated that:(1) compared to 2005, water conservation amount increased substantially in 2010. However, the overall water conservation capacity was low.(2) Among the various land cover types in Beijing, the average water conservation capacity decreased in the following order: wetland, forest, grassland, cropland, bare land and artificial surface.(3) The average water conservation amount in the main functional areas of Beijing varied substantially and was positive only in the ecological conservation area(ECA).(4) The water conservation capacity of each district and county varied substantially within ECA, among which the contribution of the forest in Miyun District, Huairou District and pinggu District was the highest. The changes in the spatiotemporal patterns of Beijing’s WCS were the synthetic effects of changes in the land covers and meteorological conditions. This study is helpful in achieving the sustainable utilization of water resources in Beijing.

关键词： water conservation services spatial and temporal patterns water balance Beijing

Observation on the Curative Effect of"Hand and Foot 12 Needle Acupuncture"on Stroke in Convalescent Period of Phlegm-Dampness Constitution

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World Journal of Integrated Traditional and Western Medicine 2022年第6期8卷 11-18页

作者： WANG Lei xiao Ding XU Yin-ping Encephalopathy Branch Pinggu Hospital of Beijing Hospital of Traditional Chinese Medicine Affiliated to the Capital Medical UniversityBeijing 101200China

Objective:To observe the clinical effects of"hand and foot 12 needle acupuncture"on stroke in convalescent period of phlegm-dampness ***:The 90 patients were divided into the treatment group(n=45)and the control group(n=45)according to the random number table *** control group received basic Western medicine treatment+rehabilitation treatment of Western medicine,and the treatment group was additively treated with"hand and foot 12 needle acupuncture"on the basis of the control *** score,MRS score and Barthel index(BI)before and after treatment were observed in the two groups,and the clinical efficacy of the two groups was ***:The clinical efficacy of stroke patients in the convalescent period of phlegm-dampness constitution after treatment:the total effective rate of the treatment group was 93.33%,and the total effective rate of the control group was 66.67%.After statistical processing,the clinical efficacy of the treatment group was significantly better than the control group;NIHSS score,MRS score and Barthel index(BI)were better than the control group,and the differences were statistically significant(P<0.05).Conclusion:"Hand and foot 12 needle acupuncture"treatment of phlegm-dampness constitution convalescent stroke clinical effect is remarkable.

关键词： Acupuncture treatment Filiform needle Hand and foot 12 needle acupuncture Phlegm-dampness constitution Convalescent stroke Treatment effect

Modulation of gut microbiota by berberine and decocted Coptis chinensis *** a high-fat diet-induced metabolic syndrome rat model

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Journal of Traditional Chinese Medical Sciences 2017年第2期4卷 149-157页

作者： Ti Huang Xu Yan xiaoguang Yan Fei Huang Jie Li Juncai xiao Yi Li Dongzhimen Hospital Beijing University of Chinese MedicineBeijing 100700China Department of Traditional Chinese Medicine Beijing HospitalBeijing 100730China BGI College Shenzhen518083China Department of Endocrinology Traditional Chinese Medicine Hospital in Pinggu DistrictBeijing101200China

Objective:To investigate the effect of decocted Coptis chinensis Franch.(Huanglian Jianji,黄连煎剂,HLJJ) and berbenne on the gut microbiota of the rats with metabolic syndrome (MS) induced by high-fat diet (HFD).Methods:Sixty Sprague Dawley rats were randomly divided into normal diet (ND) group (n =15)and HFD group (n =45).After 8 weeks of feeding,the HFD group was further divided into three groups:a group provided with a combination of HLJJ and HFD for 4 weeks,a group provided with a combination of berberine and HFD for 4 weeks,and a group supplied only with HFD for 4 consecutive *** fat weight,serum high-density lipoproteincholesterohC (HDL-C),lowdensity lipoproteincholesterol-C (LDL-C) and irisin levels were *** addition,the V3-V4 region of the cecum microbiota 16S rRNA gene amplicon was sequenced on the Illumina MiSeq ***:MS was successfully induced in the rats with HFD for 8 *** 12 weeks,serum irisin levels in the HFD + berberine group were significantly increased compared with those in the HFD group (P <.05).The high-quality tags were delineated into 1149 operational taxonomic units at a 97% similarity ***,at the genus level,Akkermansia muciniphila (***),Bacteroides,and Ruminococcus were markedly increased,whereas Candidatus arthromitus,Prevotella,Phascolarctobacterium were in great decrease in the HLJJ group and the berberine group,compared with the HFD *** abundance of ***,Ruminococcus gnavus and Bacteroides was significantly higher in the berberine group,whereas,the abundance of Oscillospira was lower in the berberine group,compared with the HLJJ ***:HLJJ and berberine changed the gut microbiota in rats with *** mechanism underlying the treatments of berberine and HLJJ on HFD-induced MS is partly different.

关键词： Metabolic syndrome Gut microbiota Decocted Coptis chinensis Franch.(HLJJ) Berberine High-fat diet