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无冻存一体化自体造血干细胞移植模式治疗新诊断多发性骨髓瘤的回顾性临床研究

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中华血液学杂志 2024年第5期45卷 488-494页

作者：杨曦李成龙陈姣车菲菲肖蓉李慧黄娟姜涛杨海清王欢况小串黄晓兵电子科技大学附属医院(四川省人民医院)血液科成都610071

目的探讨无冻存一体化自体造血干细胞移植模式在多发性骨髓瘤(MM)患者中的疗效和安全性。方法纳入2020年7月31日至2022年12月31日在电子科技大学附属医院四川省人民医院接受自体造血干细胞移植的新诊断多发性骨髓瘤(NDMM)患者96例, 对... 详细信息

目的探讨无冻存一体化自体造血干细胞移植模式在多发性骨髓瘤(MM)患者中的疗效和安全性。方法纳入2020年7月31日至2022年12月31日在电子科技大学附属医院四川省人民医院接受自体造血干细胞移植的新诊断多发性骨髓瘤(NDMM)患者96例, 对其临床资料进行回顾性分析。41例患者接受无冻存一体化移植模式(观察组), 造血干细胞动员采集后冷藏于医用输血冰箱(4 ℃)并立即启动美法仑预处理, 预处理结束24 h后回输自体造血干细胞;55例患者接受传统移植模式(对照组), 造血干细胞动员采集后液氮冷冻保存, 择期启动移植流程。两组患者均采用g-CSF联合普乐沙福进行自体造血干细胞动员。结果①观察组移植前疾病状态为非常好的部分缓解(VgPR)及完全缓解(CR)患者占比显著高于对照组[82.9%(34/41)对60.0%(33/55), P=0.016]。②与对照组相比, 观察组1级口腔黏膜炎的发生率较高(P<0.001), 但2、3级口腔黏膜炎的发生率较低(P=0.004, P=0.048), 两组均未发生≥4级口腔黏膜炎;观察组1级腹泻的发生率较高(P=0.002), 3级腹泻的发生率较低(P=0.007), 4级腹泻的发生率差异无统计学意义(P=0.506), 两组均未发生5级腹泻。③观察组细菌感染发生率低于对照组(34.1%对65.5%, P=0.002), 真菌感染(29.3%对31.4%, P=0.863)、病毒感染(4.88%对3.64%, P=0.831)发生率差异无统计学意义。④观察组与对照组粒细胞植入时间和血小板植入时间差异无统计学意义[10(8~20)d对11(8~17)d, P=0.501;13(10~21)d对15(10~20)d, P=0.245]。⑤移植后100 d前所有患者均未使用来那度胺治疗。移植后30 d, 观察组CTL、NK、Th细胞计数低于对照组(P<0.001, P=0.049, P=0.002), NKT细胞计数高于对照组(P=0.024)。移植后100 d, 观察组CTL、NKT、Th细胞计数高于对照组(P=0.025, P=0.011, P=0.007), NK细胞计数两组差异无统计学意义(P=0.396)。⑥中位随访18(4~33)个月, 观察组和对照组移植后2年总生存率分别为91.5%、78.2%(P=0.337), 无复发生存率分别为85.3%、77.6%(P=0.386), 累积复发率分别为9.8%、16.9%(P=0.373)。结论无冻存一体化自体造血干细胞移植模式在NDMM中可获得与传统移植模式相似的疗效, 重度黏膜炎和感染的发生率低于传统移植模式。

关键词：多发性骨髓瘤自体造血干细胞移植无冻存一体化移植模式

Expression and Characterization of Human Heart Type Fatty Acid Binding Protein in Pichia Pastoris

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chemical Research in Chinese Universities 2006年第2期22卷 157-161页

作者： HOU Wei TAN Yan XU Shu-fen YANg xiao-hong ZHANg Shu-hua LIU Ling-li che Yuan-yuan LIU Li-hua Pharmacy College of Jilin University The First Hospital of Jilin University Changchun 130021 P. R. China Changchun University of Science and Technology Changchun 130021 P. R. China

H-FABP is regarded as a tissue-specific protein existing only in myocardial cells. It is released from the cardiac tissue and gets into the plasma when a heart attack occurs; the myocardial infarction is a good case in point. As a resuit, the detection of H-FABP will be an early and important biomarker for the disease concerned. The objective of the study is to prepare the recombinant H-FABP by aeukaryotic expression system, pichia, to produce the protein mimicking natural H-FABP, as an immunogen for the production of the specific antibody. A gene fragment encoding H-FABP was cloned in the expressing vector pPICZα, after sequencing. The recombinant plasmid was transformed into the competent cells of the X-33 strain by means of electroporation. The expression of the target peptide induced by methanol was screened by means of Western hlotting, with the available MAb（Clone 6B6）. Highly expressive engineer strains were obtained. The production of recombinant H-FABP under induction was about 0.7 g/L, with an Mr of 14.5 kDa and recognized by a commercially available MAb （Clone 6B6）. The recombinant vector was successfully constructed. Following this, H-FABP was expressed in X-33, and it would become the source of the preparation of specific antibodies, to develop diagnostic kits.

关键词： Human heart type fatty acid binding protein Expression and purification Pichia

Complexity of the NWA 773 Clan: New Evidence from Lunar Olivine gabbro NWA 6950

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Journal of Earth Science 2023年

作者： Qi He Long xiao Ioannis Baziotis xiaochao che Yuqi Qian Jiawei Zhao Beijing SHRIMP Center， Institute of geology， Chinese Academy of geological Sciences Planetary Science Institute， School of Earth Sciences， China University of geosciences 2. Laboratory of Mineralogy and geology， Agricultural University of Athens

NWA 6950 is a type of cumulate gabbro meteorite that displays features indicating a lunar origin. Specifically， the Fe/Mn values of olivines and pyroxenes in the meteorite suggest a lunar origin， as does the presence of Fe-Ni metal. The meteorite has also undergone intense shock metamorphism， which is evidenced by the presence of ringwoodite， tuite， and xieite (a type of chromite with a CaTi2O4structure) within the shock melt veins (SMVs).The texture， mineral modal abundances， and bulk compositions (measured from the SMVs) of NWA 6950 are similar to those of the NWA 773 clan， as are the concentrations and patterns of rare-earth-elements in olivine， pyroxene， plagioclase， and phosphate. In-situ U-Pb dating of baddeleyite and phosphate in NWA 6950 has determined its crystallization age to be 3 133 ± 11 Ma and 3 129 ± 23 Ma， which is consistent with age data provided by Shaulis et al. (2017).Further， the chronology of the NWA 773 clan appears to be at least bimodal when considering the age of NWA 3333 (3 038 ± 20 Ma; Merle et al.， 2020). The tight range of ages for the NWA 773 clan at approximately 3.1 ga coincides with a change in the eruption flux and style on the Moon. This suggests that lunar volcanism may have shifted from extrusive-dominated to intrusive-dominated at approximately 3.1 ga， resulting in the widespread distribution of gabbro lithologies on the Moon.

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Changes in brain-derived neurotrophic factor expression after transplanting microencapsulated sciatic nerve cells of rabbits into injured spinal cord of rats

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Neural Regeneration Research 2007年第10期2卷 582-586页

作者： Wen xue Fu xiao hua Liu xiao lin Huang Yao fang Yang Xiang xin che Wei nie Xiang Hui yong Jiang De ming Liu Department of Anatomy Medical CollegeJiujiang UniversityJiujiang332000JiangxiProvinceChina Department of Internal Medicine Affiliated HospitalJiujiang UniversityJiujiang332000Jiangxi ProvinceChina Department of Anatomy Medical College of Nanchang UniversityNanchang 330006JiangxiProvinceChina

Changes of brain-derived neurotrophic factor （BDNF） expression reflect function of nerve cells; meanwhile, they play a significant role in researching interventions on plerosis of nerve injury. OBJECTIVE： To observe and compare the effects on changes of BDNF expression in rats with spinal cord injury between microencapsulated sciatic nerve cells of rabbits and only transplanting sciatic nerve cells of rabbits. DESIgN： Randomized controlled animal study. SETTINg： Medical School of Jiujiang College. MATERIALS： The experiment was carried out in the Medical Science Researching Center, Jiujiang College from May 2004 to May 2006. A total of 90 healthy adult SD rats, weighing 250 - 300 g, of either gender; and 10 rabbits, weighing 2.0 - 2.5 kg, of either gender, were provided by Jiangxi Experimental Animal Center. METHODS： Sciatic nerve tissue of rabbits was separated to make cell suspension, After centrifugation, suspension was mixed with 15 g/L alginate saline solution and ejaculated to 20 mmol/L barium chloride saline solution by double-cavity ejaculator. The obtained cell microcapsules were suspended in saline. Rats were randomly divided into microencapsulated group, only suspension group, and only injured group with 30 animals in each group. After anesthesia, T10 spinous process and vertebra lamina of rats in the former two groups were exposed. Spinal cord tissue in 2-mm length was removed from rats by spinal cord right hemi-section. The gelatin sponges with the size of 2 mm × 2 mm × 2 mm were grafted as filing cage, and absorbed 10 μL microencapsulated sciatic nerve cells of rabbit in the microencapsulated group and 10 μL sciatic nerve cells of rabbits in the only suspension group; respectively. No graft was placed in the only injured group. MAIN OUTCOME MEASURES： On the 1^st, 3^rd, 7^th, 14^th and 28^th days after operation, immunohistochemistry （SABC technique） was used to detect distribution and amount of positive-reactive neurons in BDNF of spinal cord samples wh

关键词： spinal cord injuries transplantation, heterologous nerve growth factors

POM/PP/pe高刚耐热复合材料的制备及性能研究

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化学工程师 2024年第6期38卷 17-20页

作者：刘潇田言李晓娟黑龙江省建筑材料工业规划设计研究院黑龙江哈尔滨150078

为获得高刚耐热聚乙烯基复合材料用于工程结构的改善和优化,本文制备了聚甲醛(POM)/聚丙烯(PP)/聚乙烯(pe)三元复合材料。采用pe.g-MAH作相容剂改善POM/PP/pe三元体系的相容性,研究pe.g-MAH对POM/PP/pe复合材料性能的影响。当pe.g-MAH... 详细信息

为获得高刚耐热聚乙烯基复合材料用于工程结构的改善和优化,本文制备了聚甲醛(POM)/聚丙烯(PP)/聚乙烯(pe)三元复合材料。采用pe.g-MAH作相容剂改善POM/PP/pe三元体系的相容性,研究pe.g-MAH对POM/PP/pe复合材料性能的影响。当pe.g-MAH添加量达到7.5份时,复合材料屈服强度达到最大值24.76MPa,提升了15.4%;冲击强度达到最大值25.78kJ·m^(-2),提升了50.7%;屈服伸长率达到最小值13.96%,降低了29.3%。结果表明,pe.g-MAH的加入提高了POM/PP/pe复合材料的综合力学性能,既改善了材料的相容性,同时也大幅提高了复合材料的储能模量,材料的刚性和耐热性能均得到了改善。

关键词： POM/PP/pe 相容性储能模量耐热性刚性

Kang-Ai Injection Inhibits gastric Cancer Cells Proliferation through IL-6/STAT3 Pathway

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Chinese Journal of Integrative Medicine 2022年第6期28卷 524-530页

作者： ZHENg Chun-lei HOU Ke-zuo WANg An-qi FANg Wan-xia YU Shi-tong LIANg Jin-e QI Hai-yan QU Xiu-juan LIU Yun-pe.g che xiao-fang Department of Medical Oncology The First Hospital of China Medical UniversityShenyang110001China Key Laboratory of Anticancer Drugs and Biotherapy of Liaoning Province The First Hospital of China Medical UniversityShenyang110001China Liaoning Province Clinical Research Center for Cancer Shenyang110001China

Objective:To explore the mechanisms underlying the proliferative inhibition of Chinese herbal medicine Kang-Ai injection(KAI)in gastric cancer ***:gastric cancer cell lines MgC803 and BgC823 were treated by 0,0.3%,1%,3%and 10%KAI for 24,48 and 72 h,*** cell proliferation was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT)*** apoptosis and cell cycle were evaluated by flow ***(IL)-6 mRNA and protein expression levels were detected by quantitative real-time polymerase chain reaction(qRT-PCR)and enzyme-linked immune sorbent assay(ELISA),*** protein expression levels of cyclin A,cyclin E,cyclin B1,cyclin D1,p21,retinoblastoma(RB),protein kinase B(AKT),extracellular regulated protein kinases(ERK),signal transducer and activator of transcription(STAT)1 and STAT3 were detected by Western ***:KAI inhibited the proliferation of MgC803 and BgC823 gastric cancer cells in dose-and time-dependent *** treated with KAI for 48 h,the proportion of g,phase was increased,expression level of cyclin D1 and phosphorylation-RB were down-regulated,whereas the expression of p21 was up-regulated(all Pg/mL significantly attenuated the proliferative effect of both 3%and 10%KAI,and recovered KAI-inhibited STAT3 phosphorylation and cyclin D1 expression level(all Pg IL-6/STAT3 signaling pathway followed by the induction of g_(1)phase arrest in gastric cancer cells.

关键词： Kang-Ai Injection gastric cancer g_(1)arrest interleukin-6 signal transducer and activator of transcription 3 Chinese herbal medicine

UM15 reinforces a lymphocyte-mimicking nanotrap for precise HIV-1 inhibition

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Nano Research 2023年第7期16卷 9906-9920页

作者： Jinbang Zhang Zhengyang Li Jiaxin Li Hui Li Junwei che Te Zhao pe.gfei Zou Jingwan Han Yang Yang Meiyan Yang Yuli Wang Wei gong Haihua xiao Zhiping Li Lin Li Chunsheng gao State key Laboratory of Toxicology and Medical Countermeasure Beijing Institute of Pharmacology and ToxicologyBeijing 100039China Pharmaceutical College Henan UniversityKaifeng 475001China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China School of Public Health and Health Management Gannan Medical UniversityGanzhou 341000China Institute of chemistry Chinese Academy of SciencesBeijing 100190China

Even the potential of T cell-mimicking nanotrap for long term viral control due to its overcoming of human immunodeficiency virus(HIV)genetic diversity and viral resistance,the robust HIV inhibition was not expected because these nanotraps displayed no obvious advantages compared with the infinite host ***,a glycoprotein 120(gp120)-targeting polypeptide UM15 reinforced lymphocyte-mimicking nanotrap was constructed,and its improved HIV-1 inhibiting efficacy was *** to the results,the constructed nanotraps exhibited evident escaping ability from uptake of the mononuclear phagocyte system and highly improved binding ability with gp120 *** constructed nanotraps neutralized all tested HIV-1 pseudo typed viruses with IC80 of 21.0μg/mL,and inhibited both X4-tropic and R5-tropic HIV-1 with IC80 of 34.4 and 20.6μg/mL,*** 40%of gp120 was observed to be shed from pseudo virus,and above 40%bystander T cells were prevented from gp120-induced death by the constructed *** safety of the constructed nanotraps was confirmed both in vitro and in ***,the constructed nanotraps could specifically neutralize free HIV-1,selectively bind with gp120 expressing HIV-1 infected cells,cause gp120 shedding,inhibit gp120-induced bystander T cell killing on the premise of safety,and were considered as promising therapeutic agents for precise inhibition of HIV.

关键词： nanotrap lymphocyte membrane UM15 human immunodeficiency virus-1(HIV-1) glycoprotein 120(gp120)

Merging bound states in the continuum by harnessing higher-order topological charges

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Light(Science & Applications) 2022年第9期11卷 2098-2106页

作者： Meng Kang Li Mao Shunping Zhang Meng xiao Hongxing Xu che Ting Chan School of Physics and Technology and Key Laboratory of Artificial Micro-and Nano-structures of Ministry of EducationWuhan UniversityWuhan 430072China Department of Physics The Hong Kong University of Science and TechnologyHong KongChina Wuhan Institute of Quantum Technology Wuhan 430206China School of Microelectronics Wuhan UniversityWuhan 430072China

Bound states in the continuum(BICs)can confine light with a theoretically infinite Q ***,in practical on-chip resonators,scattering loss caused by inevitable fabrication imperfection leads to finite Q factors due to the coupling of BICs with nearby radiative *** multiple BICs can improve the robustness of BICs against fabrication imperfection by improving the Q factors of nearby states over a broad wavevector *** date,the studies of merging BICs have been limited to fundamental BICs with topological charges±*** we show the unique advantages of higher-order BICs(those with higher-order topological charges)in constructing merging *** multiple BICs with a higher-order BIC can further improve the Q factors compared with those involving only fundamental *** addition,higher-order BICs offer great flexibility in realizing steerable off-T merging BICs.A higher-order BIC at F can split into a few off-T fundamental BICs by reducing the system *** split BICs can then be tuned to merge with another BIC,e.g.,an accidental BIC,at an off-Γ*** the in-plane mirror symmetry is further broken,merging BICs become steerable in the reciprocal *** BICs provide a paradigm to achieve robust ultrahigh-Q resonances,which are important in enhancing nonlinear and quantum effects and improving the performance of optoelectronic devices.

关键词： topological charges states

Tojapride Reverses Esophageal Epithelial Inflammatory Responses on Reflux Esophagitis Model Rats

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Chinese Journal of Integrative Medicine 2021年第8期27卷 604-612页

作者： YIN xiao-lan ZHONg Linda LIN che.g-yuan SHI xiao-shuang ZHANg Jiao cheN Zheng-yi che Hui MA Xiang-xue TIAN Ya-xin DUAN Yuan-zhi LU Lin Jl Hai-jie ZHAO Ying-pan TANg Xu-dong WANg Feng-yun Department of gastroenterology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing100091China School of Chinese Medicine Hong Kong Baptist UniversityHong Kong Special Administrative RegionKowloonChina Department of gastroenterology Changping Hospital of Traditional Chinese MedicineBeijing102200China Department of Traditional Chinese Medicine Dongfeng General HospitalShiyanHubei Province442000China Center of Clinical Pharmacology Xiyuan HospitalChina Academy of Chinese Medical SciencesBeijing100091China

Objective To investigate the mechanism of Tojapride,a Chinese herbal formula extract,on strengthening the barrier function of esophageal epithelium in rats with reflux esophagitis(RE).Methods Ten out of 85 SD rats were randomly selected as the sham group(n10),and 75 rats were developed a reflux esophagitis model(RE)by the esophageal and duodenal side-to-side *** successful modeling rats were divided into different medicated groups through a random number table including the model,low-,medium-,and high-dose of Tojapride as well as omeprazole groups(n10).Three doses of Tojapride[5.73,11.46,22.92 g/(kg•d)]and omeprazole[4.17 mg/(kg•d)]were administrated intragastrically twice daily for 3 *** the rats in the sham and model groups were administered 10 mL/kg distilled *** fluid was collected and the supernatant was kept to measure for volume,pH value and *** tissues were isolated to monitor the morphological changes through hematoxylin-eosin(HE)staining,and esophageal epithelial ultrastructure was observed by transmission electron *** expressions of nuclear factor kappa-light-chain-enhancer of activated B cells p65(NF-KBp65),κB kinase beta(IKKß),occludin,and zonula occludens-1(ZO-1)in the esophageal tissues were measured by immunohistochemistry and Western blot,*** The gastric pH value in the model group was significantly lower than the sham group(Pgroup,gastric pH value in the omeprazole and medium-dose of Tojapride groups were significantly higher(Pge area of ulceration was found on the esophageal mucosa from the model rats,while varying degrees of congestion and partially visible erosion was observed in the remaining *** increase in cell gap width and decrease in desmosome count was seen in RE rats and the effect was reversed by Tojapride *** with the sham group,the IKKßlevels were significantly higher in the model group(P<0.0

关键词： reflux-esophagitis tojapride esophageal epithelial barrier anti-inflammatory