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World Journal of Gastroenterology 2005年第12期11卷 1837-1842页

作者： xiao-nancui Jian-WuTang LiHOU BoSong LiLi Ji-WeiLiu DepartmentofOncology the1stAffilitateHospitalofDalianMedicalUniversityDalian116011LiaoningProvinceChina DepartmentofPathology DalianMedicalUniversityDalian116027LiaoningProvinceChina

AIM: To screen genes differentially expressed in mouse hepatocarcinoma ascites cell line with high potential of lymphatic ***: A subtracted cDNA library of mouse hepatocarcinoma cell line with high potential of lymphatic metastatic Hca-F and its synogenetic cell line Hca-P with a low metastatic potential was constructed by suppression subtracted hybridization(SSH) method. The screened clones of the subtracted library were sequenced and GeneBank homology search was ***: Fourteen differentially expressed cDNA fragments of Hca-F were obtained with two novel ***: SSH is a useful technique to detect differentially expressioned genes and an effective method to clone novel genes.

关键词： Suppression subtracted hybridization Liver neoplasm Lymphogenous metastasis

Identify lymphatic metastasis-associated genes in mouse hepatocarcinoma cell lines using gene chip

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World Journal of Gastroenterology 2005年第10期11卷 1463-1472页

作者： BoSong Jian-WuTang BoWang xiao-nancui LiHou LuSun Li-MinMao Chun-HuiZhou YueDu Li-HuiWang Hua-XinWang Ren-ShuZheng LeiSun DepartmentofPathology DalianMedicalUniversityDalian116027LiaoningProvinceChina ShanghaiJingtaiBiotechnologyCorporationLtd Shanghai200000China

AIM: In order to obtain lymphogenous metastasisassociated genes, we compared the transcriptional profiles of mouse hepatocarcinoma cell lines Hca-F with highly lymphatic metastasis potential and Hca-P with low lymphatic metastasis ***: Total RNA was isolated from Hca-F and Hca-P cells and synthesized into double-stranded cDNA. In vitro transcription double-stranded cDNA was labeled with biotin (i.e. biotin-labeled cRNA, used as the probe). The cRNA probes hybridized with Affymetrix GeneChip() MOE430A (containing 22 690 transcripts, including 14 500 known mouse genes and 4 371 ESTs) respectively and the signals were scanned by the GeneArray Scanner. The results were then analyzed by ***: Out of the 14 500 known genes investigated,110 (0.8%) were up regulated at least 23 fold. Among the total 4 371 ESTs, 17 ESTs (0.4%) (data were not presented) were up regulated at least 23 fold. According to the Gene Ontology and TreeView analysis, the 110genes were further classified into two groups: differential biological process profile and molecular function ***: Using high-throughput gene chip method,a large number of genes and their cellular functions about angiogenesis, cell adhesion, signal transduction, cell motility, transport, microtubule-based process, cytoskeleton organization and biogenesis, cell cycle, transcription,chaperone activity, motor activity, protein kinase activity,receptor binding and protein binding might be involved in the process of lymphatic metastasis and deserve to be used as potential candidates for further *** D1, Fosl1, Hsp47, EGFR and AR, and Cav-1 are selected as the possible candidate genes of the metastatic phenotype, which need to be validated in later *** (data were not presented) might indicate novel genes associated with lymphatic metastasis. Validating the function of these genes is helpful to identify the key or candidate gene/pathway responsible for lymphatic m

关键词： Hepatocarcinoma Lymphatic metastasis Cell lines Hca-F and Hca-P Gene chip

Construction and selection of subtracted cDNA library of mouse hepatocarcinoma cell lines with different lymphatic metastasis potential

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World Journal of Gastroenterology 2004年第16期10卷 2318-2322页

作者： LiHou Jan-WuTang xiao-nancui BoWang BoSong LeiSun DepartmentofPathology DalianMedicalUniversityDalian116027LiaoningProvinceChina

AIM: In order to elucidate the molecular mechanism of lymphatic metastasis of hepatocarcinoma, we detected the difference of gene expression between mouse hepatocarcinoma cell lines Hca-F and Hca-P with different lymphatic metastasis ***: cDNA of Hca-F cells was used as a tester and cDNA of Hca-P cells was used as a driver, cDNAs highly expressed in Hca-F cells were isolated by the suppression subtractive hybridization (SSH) method. The isolated cDNA was cloned into T/A cloning vector. The ligation products were transformed into DH5 α competent cells. Individual dories were randomly selected and used for PCR *** DNA from positive clones was isolated for ***: There were 800 positive clones in amplified subtracted cDNA library. Random analysis of 160 clones with PCR showed that 95% of the clones contained 100-700 bp inserts. Analysis of 20 sequenced cDNA clones randomly picked from the SSH library revealed 4 known genes(mouse heat shock protein 84 ku, DNA helicase, ribosomal protein S13 ,ethanol induced 6 gene) and 3 expressed sequence tags (ESTs). Four cDNAs showed no homology and presumably represent novel ***: A subtracted cDNA library of differentially expressed genes in mouse heptocarcinoma cell lines with different lymphatic metastasis potential was successfully constructed with SSH and T/A cloning techniques. The library is efficient and lays a solid foundation for searching new lymphatic metastasis related genes. The expression of mouse heat shock protein gene, DNA helicase and other 4 novel gene may be different between mouse heptocarcinoma cell lines with different lymphatic metastasis potential.

关键词： cDNA图书馆肝癌老鼠癌细胞系统淋巴腺转移肿瘤

Surface Polarization Effects on Collapse Transition of Polyelectrolyte Brushes

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Chinese Journal of Polymer Science 2024年第9期42卷 1313-1320,I0006页

作者： xiao-Fei Xu State Key Laboratory of Chemical Engineering and School of Chemical Engineering East China University of Science and TechnologyShanghai200237China

Polyelectrolyte brushes(PEBs)are commonly used to modify surface that have attracted great research *** dielectric permittivity of the grafted surface is typically significantly different from that of solution,which results in surface polarization(SP)effect with a jump of electric *** is thus important to study how SP alters the PEB’s structure and *** this work,the SP effects on PEB structure was studied using a statistical thermodynamic *** free energy functional to describe SP effect was constructed by using the image-charge ***,the electrostatic potential was solved from a modified Poisson-Boltzmann equation taking the ion solvation effect into *** the absence of SP,the thickness of PEB exhibited a continuous collapse transition when decreasing the solvent *** the presence of SP,the collapse became a jump-like *** energy analysis showed that the long-range Coulombic interaction dominated the transition because of the enhanced counterion condensation in the presence of *** theory provides an effective tool to study SP effect on PEBs,and the results explain the underlying physics in PEB collapse transition.

关键词： Surface polarization Polyelectrolyte brush Image charge Ion solvation Polyelectrolyte collapse transition

Bioinspired nanomaterials for wearable sensing and human–machine interfacing

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Nano Research 2024年第2期17卷 445-461页

作者： Vishesh Kashyap Junyi Yin xiao xiao Jun Chen Department of Bioengineering University of CaliforniaLos AngelesLos AngelesCA 90095USA Department of Mechanical and Aerospace Engineering University of CaliforniaLos AngelesLos AngelesCA 90095USA

The inculcation of bioinspiration in sensing and human–machine interface(HMI)technologies can lead to distinctive characteristics such as conformability,low power consumption,high sensitivity,and unique properties like self-healing,self-cleaning,and *** sensing and HMI are fields rife with opportunities for the application of bioinspired nanomaterials,particularly when it comes to wearable sensory systems where biocompatibility is an additional *** review discusses recent development in bioinspired nanomaterials for wearable sensing and HMIs,with a specific focus on state-of-the-art bioinspired capacitive sensors,piezoresistive sensors,piezoelectric sensors,triboelectric sensors,magnetoelastic sensors,and electrochemical *** also present a comprehensive overview of the challenges that have hindered the scientific advancement in academia and commercialization in the industry.

关键词： bioinspired nanomaterials human–machine interface wearable sensors wearable bioelectronics

Decentralized Optimal Control and Stabilization of Interconnected Systems With Asymmetric Information

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IEEE/CAA Journal of Automatica Sinica 2024年第3期11卷 698-707页

作者： Na Wang xiao Liang Hongdan Li xiao Lu the College of Electrical Engineering and Automation Shandong University of Science and TechnologyQingdao 266000China the School of Automation and Electrical Engineering Linyi UniversityLinyi 276000China the College of Energy Storage Technology Shandong University of Science and TechnologyQingdao 266000China

The paper addresses the decentralized optimal control and stabilization problems for interconnected systems subject to asymmetric *** with previous work,a closed-loop optimal solution to the control problem and sufficient and necessary conditions for the stabilization problem of the interconnected systems are given for the first *** main challenge lies in three aspects:Firstly,the asymmetric information results in coupling between control and estimation and failure of the separation ***,two extra unknown variables are generated by asymmetric information(different information filtration)when solving forward-backward stochastic difference ***,the existence of additive noise makes the study of mean-square boundedness an *** adopted technique is proving and assuming the linear form of controllers and establishing the equivalence between the two systems with and without additive noise.A dual-motor parallel drive system is presented to demonstrate the validity of the proposed algorithm.

关键词： Asymmetric information decentralized control forwardbackward stochastic difference equations interconnected system stalibization

Stretchable fiber strain sensors for wearable biomonitoring

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National Science Review 2024年第7期11卷 70-71页

作者： Shumao Xu xiao xiao Jun Chen Department of Bioengineering University of California

Wearable bioelectronics have gained significant attention since they may play a role in transforming current healthcare from a reactive and disease-centric model to a personalized one with a focus on disease preventio...

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Investigation of mechanical and electrical characteristics of selfsensing pneumatic torsional actuators

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Science China(Technological Sciences) 2024年第1期67卷 130-142页

作者： xiao Wei HU DeAn HU GuoLiang xiao YiHua Key Laboratory of Conveyance and Equipment Ministry of EducationEast China Jiaotong UniversityNanchang 330013China Key Laboratory of Advanced Design and Simulation Techniques for Special Equipment Ministry of EducationHunan UniversityChangsha 410082China

Soft pneumatic actuators are one of the most promising actuation for soft robots,and great achievements have been *** it remains challenging to endow sensing capabilities to pneumatic actuators,especially for the sensing ability originating directly from the actuator ***,a self-sensing pneumatic torsional actuator(SPTA)is designed based on the electromagnetic induction effect and magnetically responsive *** SPTA can generate feedback voltage and current with the deformation,in which the sensing function comes from its inherent *** investigate the mechanical and electrical characteristics,an experimental platform and a finite element model are established,*** find that the torsion angle and output torque increase in nonlinear with the actuating *** maximum torsion angle is 66.35°,which is 84.34%of that for the actuator fabricated by pure *** maximum output torque(24.9 N mm)improves by 23.19%compared with the actuator made by pure *** regards the electrical characteristics,the maximum feedback voltage and current are 2.90μV and 29.50 nA when the SPTA is actuated by a pressure of−40 *** also demonstrate that the relationship between the torsion angle and the magnetic flux change is approximately ***,the number of turns of wires,magnetic powders contents,and magnetic direction on the feedback voltage and current are *** show that the feedback voltage and current can be enhanced by increasing the number of turns and magnetic powders *** envision that the SPTA would be promising for soft robots to realize their accuracy control and intelligentization.

关键词： pneumatic torsional actuators self-sensing feedback voltage finite element modeling soft robots

Classification of knowledge graph completeness measurement techniques

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Journal of Systems Engineering and Electronics 2024年第1期35卷 154-162页

作者： ZHANG Ying xiao Gang Institute of Systems Engineering Academy of Military SciencesBeijing 100107China

At present,although knowledge graphs have been widely used in various fields such as recommendation systems,question and answer systems,and intelligent search,there are always quality problems such as knowledge omissions and *** assessment and control,as an important means to ensure the quality of knowledge,can make the applications based on knowledge graphs more complete and more accurate by reasonably assessing the knowledge graphs and fixing and improving the quality problems at the same ***,as an indispensable part of the knowledge graph construction process,the results of quality assessment and control determine the usefulness of the knowledge *** them,the assessment and enhancement of completeness,as an important part of the assessment and control phase,determine whether the knowledge graph can fully reflect objective phenomena and reveal potential connections among *** this paper,we review specific techniques of completeness assessment and classify completeness assessment techniques in terms of closed world assumptions,open world assumptions,and partial completeness *** purpose of this paper is to further promote the development of knowledge graph quality control and to lay the foundation for subsequent research on the completeness assessment of knowledge graphs by reviewing and classifying completeness assessment techniques.

关键词： quality assessment completeness assessment closed world assumptions open world assumption partial completeness assumption

Oxygen-assisted zinc recovery from electric arc furnace dust using magnesium chloride

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International Journal of Minerals,Metallurgy and Materials 2024年第10期31卷 2300-2311页

作者： Jingdong Huang xiao Yang School of Materials Science and Engineering Zhejiang UniversityHangzhou 310027China School of Engineering Westlake UniversityHangzhou 310030China

Electric arc furnace(EAF)dust is an important secondary resource containing metals,such as zinc(Zn)and iron(Fe).Recover-ing Zn from EAF dust can contribute to resource recycling and reduce environmental ***,the high chemical stability of ZnFe_(2)O_(4)in EAF dust poses challenges to Zn *** address this issue,a facile approach that involves oxygen-assisted chlorination using molten MgCl_(2)is *** work focused on elucidating the role of O2 in the reaction between ZnFe_(2)O_(4)and molten MgCl_(2).The results demonstrate that MgCl_(2)effectively broke down the ZnFe_(2)O_(4)structure,and the high O2 atmosphere considerably promoted the sep-aration of Zn from other components in the form of ZnCl_(2).The presence of O2 facilitated the formation of MgFe_(2)O_(4),which stabilized Fe and prevented its ***,the excessive use of MgCl_(2)resulted in increased evaporation loss,and high temperatures pro-moted the rapid separation of *** on these findings,we successfully extracted ZnCl_(2)-enriched volatiles from practical EAF dust through oxygen-assisted *** optimized conditions,this method achieved exceptional Zn chlorination percentage of over 97%within a short period,while Fe chlorination remained below 1%.The resulting volatiles contained 85wt%of ZnCl_(2),which can be further processed to produce metallic *** findings offer guidance for the selective recovery of valuable metals,particularly from solid wastes such as EAF dust.

关键词： electric arc furnace dust zinc oxygen magnesium chloride chlorination