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Cyclin Dl antisense oligodexoyneucleotides inhibits growth and enhances chemosensitivity in gastric carcinoma cells

World Journal of Gastroenterology 2006年第11期12卷 1766-1769页

作者： xiao-ming Shuai Gao-xiong Han Guo-Bin Wang Jun-Hua Chen Department of Gastroenterologic Surgery Union HospitalTongji Medical College Huazhong University of Science and Technology Wuhan 430022 Hubei Province China

AIM： To examine the effects of cyclin D1 antisense oligodexoyneucleotides （ASODN） on growth and chemosensitivity of gastric carcinoma cell lines SGC7901 and its mechanism. METHODS： Phosphorothioate modified cyclin D1 ASODN was encapsulated by LipofectAMINE2000 （LF2000） and transfected into cells, the dose-effect curves and growth curves were observed. 5-FU, MTX, CDDP of different concentrations were given after transfecting cells with cyclin D1 ASODN for 24 h, the dose-effect responses were observed and IC50s were calculated. The mRNA expression of cyclin D1, thymidylate synthase （TS）, thymidine phosphorylase （TP） and dihydrofolate reductase （DHFR） was detected by reverse transcription-PCR （RTPCR） at 24 h and 48 h after transfection. RESULTS： Dose-dependent inhibitory effect was caused by cyclin D1 ASODN in SGC7901 cells. Transfecting gastric carcinoma cells with 0.2 μmol/L cyclin D1 ASODN for 24 h could inhibit growth significantly and reduce expression of cyclin D1 mRNA. Cyclin D1 ASODN could increase the chemosensitivity to 5-FU, MTX, CDDP in cells, The IC50s of different chemotherapeutic agents in ASODN plus chemotherapy groups were significantly lower than those in controls. Transfection with cyclin D1 ASODN leaded to an increase in TS and DHFR mRNA and a decrease in TP mRNA as determined by RT-PCR at 24 h, the alterations were more significant at 48 h. CONCLUSIONS： Cyclin D1 ASODN can decrease mRNA expression of cyclin D1, inhibit growth and enhance the chemosensitivity by changing the expression of enzymes related to metabolism of chemotherapeutic agents in SGC7901 gastric carcinoma cells.

关键词： Gastric carcinoma Cyclin D1 Antisense oligodexoyneucleotides Chemosensitivity

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Platelet RNA signature independently predicts ovarian cancer prognosis by deep learning neural network model

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Protein & Cell 2023年第8期14卷 618-622页

作者： Chun-Jie Liu Hua-Yi Li Yue Gao Gui-Yan Xie Jian-Hua Chi Gui-Ling Li Shao-Qing Zeng xiao-ming xiong Jia-Hao Liu Lin-Li Shi xiong Li xiao-Dong Cheng Kun Song Ding Ma An-Yuan Guo Qing-Lei Gao Department of Gynecological Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China National Clinical Research Center for Obstetrics and Gynecology Cancer Biology Research Center(Key Laboratory of the Ministry of Education)Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Center for Artificial Intelligence Biology Hubei Bioinformatics&Molecular Imaging Key LaboratoryKey Laboratory of Molecular Biophysics of the Ministry of EducationCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Gynecology and Obstetrics The Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Gynecological Oncology Women's HospitalSchool of MedicineZhejiang UniversityHangzhou 310011China Gynecological Oncology Key Laboratory Qilu HospitalShandong UniversityJinan 250100China

Dear Editor,Platelets are circulating anucleate cytoplasmic fragments of megakaryocytes and characterized by their functions in wound healing and vascular integrity *** evidence highlights the extensive reciprocal signaling interactions between platelets and tumor cells(Haemmerle et al.,2018).Tumor cells activate and aggregate platelets to sustain proliferation(Cho et al.,2012),resist apoptosis,and promote metastasis(Haemmerle et al.,2017).

关键词： Platelet healing

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基于高斯分解的多尺度3D Otsu阈值分割算法

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吉林大学学报（工学版） 2017年第1期47卷 255-261页

作者：肖明尧李雄飞长春师范大学计算机科学与技术学院长春130032 吉林大学计算机科学与技术学院长春130012

针对阈值图像分割算法对噪音敏感的问题,提出了一种新的基于分解的Otsu阈值分割算法。整个分割算法为一个迭代过程,在每次迭代中,该图像首先用3D Otsu算法进行分割,然后利用高斯核函数对原图像进行滤波,得到一个平滑的图像,然后被输入... 详细信息

针对阈值图像分割算法对噪音敏感的问题,提出了一种新的基于分解的Otsu阈值分割算法。整个分割算法为一个迭代过程,在每次迭代中,该图像首先用3D Otsu算法进行分割,然后利用高斯核函数对原图像进行滤波,得到一个平滑的图像,然后被输入到下一个迭代中。最后,合并每次迭代过程中产生的分割结果,获得最终的分割结果。该算法的优点在于分割结果稳定,且具有较强的抗噪性。本文在MR大脑图像上进行实验,结果表明,该算法优于其他同类阈值分割算法。

关键词：计算机应用图像分割 Otsu算法噪音高斯分解

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Protective effects of cariporide on endothelial dysfunction induced by high glucose

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Acta Pharmacologica Sinica 2005年第3期26卷 329-333页

作者： Shuang-xi WANG xiao-ming xiong Tao SONG Li-ying LIU~2 Department of Pharmacology,Pharmaceutical College,Central South Universi~ Changsha 410078,China DepartmentofPharmacology PharmaceuticalCollegeCentralSouthUniversityChangsha410078China

Aim:To explore the effects of cariporide,a selective sodium-hydrogen antiporterinhibitor,on endothelial dysfunction induced by high ***:Acetyl-choline (ACh)-induced endothelium-dependent relaxation (EDR),sodium nitro-prusside (SNP)-induced endothelium-independent relaxation and biochemical pa-rameters including malondialdehyde (MDA),superoxide dismutase (SOD),andnitric oxide (NO) were measured in rat isolated ***:A 6-h incubationof aortic rings with high glucose (44 mmol/L) resulted in a significant inhibitionof EDR,but had no effects on endothelium-independent *** the 6-hincubation of aortic rings in the co-presence of cariporide (0.01,0.1,and 1 μmol/L)with high glucose,cariporide prevented the inhibition of EDR caused by highglucose in concentration-dependent ***,high glucose decreasedSOD activity and contents of NO,and increased MDA concentration in *** (1 μmol/L) significantly resisted the decrease of NO contentand SOD activity,and elevation of MDA concentration caused by high glucose inaortic *** (44 mmol/L) or cariporide (1 μmol/L) alone had no ef-fect on EDR,endothelium-independent relaxation and biochemical ***:Cariporide significantly prevented endothelial dysfunction inducedby high *** mechanisms of endothelial dysfunction induced by highglucose may involve the activation of sodium-hydrogen antiporter and the gen-eration of oxygen-free radicals,but it is not related to the change of osmolarity.

关键词： sodium-hydrogen antiporter endothelium glucose nitric oxide malondialdehyde superoxide dismutase

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Enhancement of motor functional recovery in thoracic spinal cord injury: voluntary wheel running versus forced treadmill exercise

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Neural Regeneration Research 2025年第3期20卷 836-844页

作者： Do-Hun Lee Dan Cao Younghye Moon Chen Chen Nai-Kui Liu xiao-ming Xu Wei Wu Spinal Cord and Brain Injury Research Group Stark Neurosciences Research InstituteIndiana University School of MedicineIndianapolisINUSA Department of Neurological Surgery Indiana University School of MedicineIndianapolisINUSA Department of Neuroscience Baylor College of MedicineHoustonTXUSA

Spinal cord injury necessitates effective rehabilitation strategies, with exercise therapies showing promise in promoting recovery. This study investigated the impact of rehabilitation exercise on functional recovery and morphological changes following thoracic contusive spinal cord injury. After a 7-day recovery period after spinal cord injury, mice were assigned to either a trained group(10 weeks of voluntary running wheel or forced treadmill exercise) or an untrained group. Bi-weekly assessments revealed that the exercise-trained group, particularly the voluntary wheel exercise subgroup, displayed significantly improved locomotor recovery, more plasticity of dopaminergic and serotonin modulation compared with the untrained group. Additionally, exercise interventions led to gait pattern restoration and enhanced transcranial magnetic motor-evoked potentials. Despite consistent injury areas across groups, exercise training promoted terminal innervation of descending axons. In summary, voluntary wheel exercise shows promise for enhancing outcomes after thoracic contusive spinal cord injury, emphasizing the role of exercise modality in promoting recovery and morphological changes in spinal cord injuries. Our findings will influence future strategies for rehabilitation exercises, restoring functional movement after spinal cord injury.

关键词： behavioral assessment motor function neural plasticity running wheel exercise spinal cord injury treadmill exercise voluntary exercise

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Platelet RNA enables accurate detection of ovarian cancer:an intercontinental,biomarker identification study

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Protein & Cell 2023年第8期14卷 579-590页

作者： Yue Gao Chun-Jie Liu Hua-Yi Li xiao-ming xiong Gui-Ling Li Sjors G.J.G.In't Veld Guang-Yao Cai Gui-Yan Xie Shao-Qing Zeng Yuan Wu Jian-Hua Chi Jia-Hao Liu Qiong Zhang xiao-Fei Jiao Lin-Li Shi Wan-Rong Lu Wei-Guo Lv Xing-Sheng Yang Jurgen M.J.Piek Cornelis D de Kroon C.A.R.Lok Anna Supernat Sylwia Łapińska-Szumczyk Anna Łojkowska Anna J Żaczek Jacek Jassem Bakhos A.Tannous Nik Sol Edward Post Myron G.Best Bei-Hua Kong Xing Xie Ding Ma Thomas Wurdinger An-Yuan Guo Qing-Lei Gao Department of Gynecological Oncology Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China National Clinical Research Center for Obstetrics and Gynecology Cancer Biology Research Center(Key Laboratory of the Ministry of Education)Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Center for Artificial Intelligence Biology Hubei Bioinformatics&Molecular Imaging Key LaboratoryKey Laboratory of Molecular Biophysics of the Ministry of EducationCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China Cancer Center Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Neurosurgery Amsterdam UMCVU University Medical CenterCancer Center AmsterdamDe Boelelaan 11171081 HV AmsterdamThe Netherlands Brain Tumor Center Amsterdam Amsterdam UMCVU University Medical CenterCancer Center AmsterdamDe Boelelaan 11171081 HV AmsterdamThe Netherlands Department of Gynecological Oncology Women's HospitalSchool of MedicineZhejiang UniversityHangzhou 310011China Gynecological Oncology Key Laboratory Qilu HospitalShandong UniversityJinan 250100China Department of Obstetrics and Gynecology Catharina HospitalMichelangelolaan 25623EJ EindhovenEindhovenThe Netherlands Department of Obstetrics and Gynecology Leiden University Medical CenterAlbinusdreef 22300 RCLeidenThe Netherlands Department of Gynecological Oncology Center of Gynecologic Oncology AmsterdamAntoni van LeeuwenhoekPlesmanlaan 1211066 CX AmsterdamThe Netherlands Laboratory of Translational Oncology Intercollegiate Faculty of BiotechnologyUniversity of Gdansk and Medical University of GdanskGdanskPoland Department of Gynecology Gynecological Oncology and Gynecological EndocrinologyMedical University of GdanskGdanskPoland Department of Oncology and Radiotherapy Medical University of GdanskGdanskPoland Department of Neurology Massachuse

Platelets are reprogrammed by cancer via a process called education,which favors cancer *** transcriptional profile of tumor-educated platelets(TEPs)is skewed and therefore practicable for cancer *** intercontinental,hospital-based,diagnostic study included 761 treatment-naive inpatients with histologically confirmed adnexal masses and 167 healthy controls from nine medical centers(China,n=3;Netherlands,n=5;Poland,n=1)between September 2016 and May *** main outcomes were the performance of TEPs and their combination with CA125 in two Chinese(VC1 and VC2)and the European(VC3)validation cohorts collectively and *** outcome was the value of TEPs in public pan-cancer platelet transcriptome *** AUCs for TEPs in the combined validation cohort,VC1,VC2,and VC3 were 0.918(95%CI 0.889-0.948),0.923(0.855-0.990),0.918(0.872-0.963),and 0.887(0.813-0.960),*** of TEPs and CA125 demonstrated an AUC of 0.922(0.889-0.955)in the combined validation cohort;0.955(0.912-0.997)in VC1;0.939(0.901-0.977)in VC2;0.917(0.824-1.000)in *** subgroup analysis,TEPs exhibited an AUC of o.858,0.859,and 0.920 to detect early-stage,borderline,non-epithelial diseases and 0.899 to discriminate ovarian cancer from *** had robustness,compatibility,and universality for *** diagnosis of ovarian cancer since it withstood validations in populations of different ethnicities,heterogeneous histoiogical subtypes,and early-stage ovarian ***,these observations warrant prospective validations in a larger population beforeclinicalutilities.

关键词： tumor-educated platelets ovarian cancer liquid biopsy preoperative diagnosis

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中华民族视觉形象的文化叙事及实践——基于文化形象学的论析

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新疆师范大学学报（哲学社会科学版） 2025年第2期46卷 112-121页

作者：令小雄苗昕西北师范大学马克思主义学院教育部高校思想政治工作队伍培训研修中心(西北师范大学) 甘肃兰州730070

文本基于多维度理论系统梳理中华民族视觉形象的文化意蕴、内涵质态与构建策略,为铸牢中华民族共同体意识提供理论基础。一是运用文化符号学理论解析中华民族视觉形象的意涵及其历史演变,探赜“和合共生,合谐共美”价值理想承载的多元... 详细信息

文本基于多维度理论系统梳理中华民族视觉形象的文化意蕴、内涵质态与构建策略,为铸牢中华民族共同体意识提供理论基础。一是运用文化符号学理论解析中华民族视觉形象的意涵及其历史演变,探赜“和合共生,合谐共美”价值理想承载的多元一体文化格局,通过提炼核心符号,揭示视觉形象塑造在铸牢中华民族共同体意识中的重要作用。二是采用历史唯物主义方法论考察中华民族视觉形象在不同历史时期的构建与传播路径,通过文化形象考古学和文献学剖析视觉形象文化功能转变与价值重塑过程,探讨其在凝聚民族力量、强化国家认同中的重要意义。三是引入文化哲学、文化心理学视角分析视觉形象在增强社会凝聚力和心理认同中的内在机制,探究视觉符号通过激活集体记忆和情感共鸣、唤起民族自豪感和共同体意识成为社会整合的重要媒介。四是基于文化形象学视角提出构建中华民族视觉形象的策略:从中华文化形象本体论和认识论出发,提炼具有代表性的符号,利用现代数智科技和新媒体创新视觉形象的表达与传播,注重文化形象多样性的辩证统一,发掘整合各民族形象文化元素的共同意识。

关键词：中华民族视觉形象国家形象文化形象认同文化传播

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GPS: graph contrastive learning via multi-scale augmented views from adversarial pooling

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Science China(Information Sciences) 2025年第1期 145-158页

作者： Wei JU Yiyang GU Zhengyang MAO Ziyue QIAO Yifang QIN xiao LUO Hui xiong ming ZHANG School of Computer Science National Key Laboratory for Multimedia Information ProcessingPeking University Artificial Intelligence Thrust The Hong Kong University of Science and Technology Department of Computer Science University of California

Self-supervised graph representation learning has recently shown considerable promise in a range of fields, including bioinformatics and social networks. A large number of graph contrastive learning approaches have shown promising performance for representation learning on graphs, which train models by maximizing agreement between original graphs and their augmented views(i.e., positive views). Unfortunately, these methods usually involve pre-defined augmentation strategies based on the knowledge of human experts. Moreover, these strategies may fail to generate challenging positive views to provide sufficient supervision signals. In this paper, we present a novel approach named graph pooling contrast(GPS) to address these *** by the fact that graph pooling can adaptively coarsen the graph with the removal of redundancy, we rethink graph pooling and leverage it to automatically generate multi-scale positive views with varying emphasis on providing challenging positives and preserving semantics, i.e., strongly-augmented view and weakly-augmented view. Then, we incorporate both views into a joint contrastive learning framework with similarity learning and consistency learning, where our pooling module is adversarially trained with respect to the encoder for adversarial robustness. Experiments on twelve datasets on both graph classification and transfer learning tasks verify the superiority of the proposed method over its counterparts.

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Cyclin D1反义寡核苷酸对SGC7901胃癌细胞生长和化疗敏感性的影响

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实用肿瘤杂志 2003年第6期18卷 437-440页

作者：帅晓明韩高雄王国斌华中科技大学同济医学院附属协和医院普外科湖北武汉430022

目的　研究 Cyclin D1反义寡核苷酸对胃癌细胞 SGC790 1生长和化疗敏感性的影响 ,并探讨其内在机制。方法　采用脂质体 lipofect AMINE2 0 0 0包裹硫代修饰的 Cyclin D1ASODN转染细胞 ,观察对 SGC790 1细胞的量效曲线及其生长曲线。 0 .... 详细信息

目的　研究 Cyclin D1反义寡核苷酸对胃癌细胞 SGC790 1生长和化疗敏感性的影响 ,并探讨其内在机制。方法　采用脂质体 lipofect AMINE2 0 0 0包裹硫代修饰的 Cyclin D1ASODN转染细胞 ,观察对 SGC790 1细胞的量效曲线及其生长曲线。 0 .2μmol/ L Cyclin D1ASODN转染细胞 2 4小时后 ,给予梯度浓度的 5 -氟尿嘧啶 (5 -FU )、氨甲喋呤 (MTX)、顺铂 (DDP) ,观察量效反应 ,计算 IC50 。应用 RT- PCR检测细胞转染后 2 4小时和 4 8小时时Cyclin D1、胸苷酸合成酶 (TS)、胸腺嘧啶磷酸化酶 (TP)、二氢叶酸还原酶 (DHFR) m RNA表达情况。结果　 CyclinD1ASODN对 SGC790 1细胞产生剂量依赖性的抑制效应 ,0 .2μm ol/ L Cyclin D1ASODN转染 2 4小时能显著抑制胃癌细胞生长 ,降低 Cyclin D1m RNA表达。 Cyclin D1ASODN增加了胃癌细胞对 5 - FU ,MTX,DDP的敏感性 ,ASODN +化疗组对各化疗药物的 IC50 均有显著下降。 RT- PCR显示 Cyclin D1ASODN转染后 2 4小时时 TS、DHFRm RNA表达较对照组下降 ,而 TPm RNA表达较对照组升高 ,4 8小时时 TS、TP、DHFR m RNA表达的改变更加明显。结论　 Cyclin D1ASODN能降低 Cyclin D1m RNA表达 ,抑制胃癌细胞 SGC790 1的生长 ,并通过改变5 - FU,MTX代谢相关酶的表达提高细胞对它们的?

关键词： CyclinD1反义寡核苷酸 SGC7901 胃癌化疗敏感性癌细胞生长

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基于颜色校正与改进的对比度增强的水下图像增强方法

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现代电子技术 2025年第1期48卷 40-46页

作者：刘明肖汉城云南民族大学电气信息工程学院云南昆明650500

针对水下介质分布不均匀引起的光吸收和散射导致图像颜色失真、对比度低、细节模糊,严重影响ORBSLAM2算法前端特征提取与匹配鲁棒性的问题,文中提出一种基于颜色校正与改进的自适应对比度增强的图像处理方法。首先,利用一种改进的颜色... 详细信息

针对水下介质分布不均匀引起的光吸收和散射导致图像颜色失真、对比度低、细节模糊,严重影响ORBSLAM2算法前端特征提取与匹配鲁棒性的问题,文中提出一种基于颜色校正与改进的自适应对比度增强的图像处理方法。首先,利用一种改进的颜色通道补偿和颜色通道拉伸方法去除色偏;其次,采用改进的自适应对比度增强方法提高图像的亮度与对比度;最后,将彩色校正图像与对比度增强图像在HSV空间中融合。此外,将提出的算法与一些其他算法进行主客观的评价,并将处理好的图片进行特征提取和匹配。结果表明,该算法处理的图片不仅在主客观评价上均优于对比算法,而且增加了特征点的匹配数量,为水下图像处理提供了借鉴。

关键词：水下视觉SLAM 水下图像增强自适应对比度增强颜色校正颜色补偿特征匹配

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