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A novel CRX mutation by whole-exome sequencing in an autosomal dominant cone-rod dystrophy pedigree

International Journal of Ophthalmology(English edition) 2015年第6期8卷 1112-1117页

作者： Qin-Kang Lu Na Zhao Ya-Su Lv Wei-Kun Gong Hui-Yun Wang Qi-Hu Tong xiao-ming lai Rong-Rong Liu ming-Yan Fang Jian-Guo Zhang Zhen-Fang Du Xian-Ning Zhang Ophthalmology Center Yinzhou People's Hospital Yinzhou Hospital Affiliated to Medical School of Ningbo University Department of Cell Biology and Medical Genetics Research Center for Molecular Medicine Institute of Cell BiologyZhejiang University School of Medicine BGI-Shenzhen

AIMTo identify the disease-causing gene mutation in a Chinese pedigree with autosomal dominant cone-rod dystrophy (adCORD).METHODSA southern Chinese adCORD pedigree including 9 affected individuals was studied. Whole-exome sequencing (WES), coupling the Agilent whole-exome capture system to the Illumina HiSeq 2000 DNA sequencing platform was used to search the specific gene mutation in 3 affected family members and 1 unaffected member. After a suggested variant was found through the data analysis, the putative mutation was validated by Sanger DNA sequencing of samples from all available family *** results of both WES and Sanger sequencing revealed a novel nonsense mutation c.C766T (p.Q256X) within exon 5 of CRX gene which was pathogenic for adCORD in this family. The mutation could affect photoreceptor-specific gene expression with a dominant-negative effect and resulted in loss of the OTX tail, thus the mutant protein occupies the CRX-binding site in target promoters without establishing an interaction and, consequently, may block *** modes of Mendelian inheritance in CORD have been observed, and genetic heterogeneity is a hallmark of CORD. Therefore, conventional genetic diagnosis of CORD would be time-consuming and labor-intensive. Our study indicated the robustness and cost-effectiveness of WES in the genetic diagnosis of CORD.

关键词： cone-rod dystrophy autosomal dominant cone-rod dystrophy whole-exome sequencing Sanger sequencing CRX gene mutation

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Site-specific conjugation of bifunctional chelator BAT to mouse IgG1 Fab~1 fragment

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Acta Pharmacologica Sinica 2006年第2期27卷 237-241页

作者： Jun LI~1 Xue-hao WANG xiao-ming WANG Zhao-lai CHEN Division of Hepatic Surgery.Department of Surgery,the First Affiliated Hospital of Nanjing Medical University and Jiangsu Province Hospital.Nanjing 210029.China Division of Hepatic Surgery Department of Surgery the First Affiliated Hospital of Nanjing Medical University and Jiangsu Province Hospital Nanjing 210029 China

Aim:To perform a site-specific conjugation of Fab’ fragments of a mouse mono-clonal antibody(MoAb)B43(of IgG1subtype)to a bifunctional chelator 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N′,N″,N′″-tetraacetic acid(BAT)via the thiol groups in the hinge distal to the antigen-binding site of the Fab~***:B43 was cleaved using a simple 2-step ***,stable F(ab~1)2was produced by pepsin ***~1 with tree thiol in thehinge region was then obtained by cysteine reduction of F(ab~1)***,a site-specific conjugation of Fab~1 to thiol-specific BAT was performed in a ***:The Fab~1 fragment had approximately 1.8 free thiol groups permolecule after cysteine *** conjugation efficiency and the chemicalyield were approximately 1.28 moles chelator/Fab′ and 74% of the initial concentra-tion of Fab′,*** F(ab′)2,Fab′ and Fab′-BAT all maintained reason-able antigen-binding properties.67Cu labeling of the conjugate under standardconditions did not impair the immunoreactivity of Fab′-***:This isa simple and efficient method for producing immunoreactive conjugates of Fab′-BAT,which can be used to make radiometal-labeled conjugates for further diag-nostic and therapeutic applications.

关键词： conjugation BAT chelator monoclonal antibody

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骨皮质切开加速大鼠正畸牙移动的机制研究

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上海口腔医学 2017年第1期26卷 12-16页

作者：朱绍跃袁长永刘宗响李晓明王鹏来徐州医学院附属徐州市口腔医院种植中心江苏徐州221002 徐州医学院口腔医学院江苏徐州221004 香港大学牙学院香港特别行政区999077 徐州医学院附属徐州市口腔医院牙周病科江苏徐州221002

目的 :研究骨皮质切开术加速大鼠正畸牙移动的组织学改建机制。方法 :选用健康未孕雌性Sprague-Dawley(S-D)大鼠48只,随机分为骨皮质切开手术组和对照组。在手术组大鼠行骨皮质切开术,对照组大鼠行对照手术后,构建正畸牙移动模型。在牙... 详细信息

目的 :研究骨皮质切开术加速大鼠正畸牙移动的组织学改建机制。方法 :选用健康未孕雌性Sprague-Dawley(S-D)大鼠48只,随机分为骨皮质切开手术组和对照组。在手术组大鼠行骨皮质切开术,对照组大鼠行对照手术后,构建正畸牙移动模型。在牙移动0、1、3、7 d分别处死2组大鼠各6只。测量牙移动距离并制备组织学切片,行抗酒石酸酸性磷酸酶(TRAP)染色和核因子κB受体活化因子配体(RANKL)免疫组织化学检测。采用SPSS16.0软件包对数据进行统计学分析。结果:骨皮质切开术可在牙移动第1天及3 d后加速正畸牙移动,而牙移动3 d时2组大鼠牙移动距离无显著差异。牙移动第3天和第7天,手术组大鼠压力区破骨细胞数目均显著高于对照组(P<0.05),手术组压力区RANKL的表达也显著高于对照组。结论:骨皮质切开术可加速大鼠正畸牙移动,这可能是由于牙周组织中RANKL高表达介导的破骨增强所致。

关键词：骨皮质切开术牙移动破骨细胞 RANKL

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鄱阳湖区能源系统网络图及其分析

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江西省科学院院刊 1984年第2期 37-46页

作者：潘阳章晓明袁桂来江西省科学院能源研究所

本文以网络图的形式,对鄱阳湖区十县一市建立了能源描述模型.通过网络图,分析了湖区的能源问题,用具体的数据定量地描述了湖区能源系统的现状和特点,并为能源研究提供了一些有用的数据.在分析的基础上,揭示了湖区能源现状所存在的问题,... 详细信息

本文以网络图的形式,对鄱阳湖区十县一市建立了能源描述模型.通过网络图,分析了湖区的能源问题,用具体的数据定量地描述了湖区能源系统的现状和特点,并为能源研究提供了一些有用的数据.在分析的基础上,揭示了湖区能源现状所存在的问题,并由此提出了一些自己的看法.分析结果表明,为了解决鄱阳湖地区的能源问题,应根据湖区的具体情况,因地制宜,走一条节能和开发并进的路.

关键词：能源能源利用率湖区自然区能源系统消费结构消费构成农村生活用能能源消费总量

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Quantitative Research on the Role of Large Precipitates in V–Ti Micro-Alloyed Steel during Dynamic Transformation

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Acta Metallurgica Sinica(English Letters) 2015年第1期28卷 77-82页

作者： Da-Yong Wu Fu-Ren xiao lai-Lei Wu ming-Yu Sun Bo Liao The Key Laboratory of Metastable Materials Science & Technology College of Materials Science and EngineeringYanshan University

To research the effect of large precipitates（size 〉 0.2 μm） on strain-induced dynamic transformation, the variation of V contents in large precipitates has been investigated quantitatively in two V–Ti micro-alloyed steels. The results showed that high N content promoted V precipitation on the surface of Ti large precipitates rapidly. Subsequently,large precipitates containing V induced the formation of intragranular ferrite, which accelerated the dynamic transformation process remarkably, promoted the occurrence of continuous dynamic recrystallization of ferrite and improved the refinement effect.

关键词： Transformation Precipitates Intragranular ferrite Recrystallization EBSD

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Study of crust-mantle transitional zone along the northeast margin of Qinghai-Xizang plateau

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Acta Seismologica Sinica(English Edition) 2004年第2期17卷 144-151页

作者： lai xiao-ling(赖晓玲) ZHANG Xian-kang(张先康) FANG Sheng-ming(方盛明) Geophysical Exploration Center China Earthquake Administration Zhengzhou 450002 China Geophysical Exploration Center China Earthquake Administration Zhengzhou 450002 China Geophysical Exploration Center China Earthquake Administration Zhengzhou 450002 China

This study deals with complexity, frequency spectrum and velocity model of the crust-mantle transitional zone in different tectonic units along the northeast margin of Qinghai-Xizang plateau, based on PmP waveform data from two deep seismic sounding profiles passing through the area. It reveals that Moho has stable tectonic features in Ordos and Lingzhong basins, where crust and mantle are coupled as first-order discontinuity. Moho shows obvious signs of activity in Haiyuan seismic region and in the contact zone between Bayanhar block and Qaidam block. Crust and mantle in these two areas are coupled as complicated crust-mantle transitional zone consisting of multiple laminae with alternate high and low velocities, totaling 20 km in thickness. The difference between Moho of different tectonic units reflects heterogeneity of the coupled crust-mantle zone; the difference between fine structures of Haiyuan seismic region and Maqin fault zone reflects different deep material composition of the two continent-continent collision zones and the interaction between blocks.

关键词： crust-mantle transitional zone tectonic units Haiyuan seismic region Maqin fault zone

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Leaching and mass transfer characteristics of elements from ion-adsorption type rare earth ore

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Rare Metals 2015年第5期34卷 357-365页

作者： Yan-Fei xiao Zong-Yu Feng Gu-Hua Hu Li Huang xiao-Wei Huang Ying-Ying Chen ming-lai Li National Engineering Research Center for Rare Earth Materials General Research Institute for Nonferrous Metals Grirem Advanced Materials Co. Ltd. Chalco Guangxi Nonferrous Rare Earth Development Co. Ltd. School of Chemical Engineering China University of PetroleumBeijing

The differences in the leaching and mass transfer characteristics of ion-adsorption type rare earth ore between rare earth and non-rare earth impurities were investigated. It is determined that the leaching kinetics of rare earths （REs）, Mg and Ca could be described by the shrinking-core model. The leaching rate is controlled by inner diffusion and is in the order of Mg 〉 Ca 〉 RE. The apparent activation energy is 8.48, 6.79 and 6.32 kJ·mol^-1, respectively. In leaching system of Al, the leaching efficiency decreases with the increase in temperature because of hydrolysis reactions. The leaching efficiency of aluminum increases firstly and then decreases with the passage of time when the temperature is higher than 35 ℃. Besides, there is a good consistency in leaching characteristics and mass transfer, and the order of the time-to- peak is Mg 〈 Ca 〈 RE 〈 Al. Therefore, rare earth and non-rare earth may be partly separated by expanding the separation coefficient. The research could provide a theoretic basis for the separation of rare earth and non-rare earth impurities. Furthermore, compound leaching with magnesium salt and calcium salt is innovatively proposed to solve the problem of calcium and magnesium nutrient loss in the soil as well as eliminate ammonia-nitrogen emissions from the source.

关键词： impurities Rare earth Leaching Non-rare earth Mass transfer

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A TRANSFER FORECASTING MODEL FOR CONTAINER THROUGHPUT GUIDED BY DISCRETE PSO

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Journal of Systems Science & Complexity 2014年第1期27卷 181-192页

作者： xiao Jin xiao Yi FU Julei lai Kin Keung Business School Sichuan University Chengdu 610064 China. School of Information Management Central China Normal University Wuhan 430079 China Academy ofMathematics and Systems Science Chinese Academy of Sciences Beijing 100190 China. Information System & Management National University of Defense Technology Changsha 410073 China. Department of Management Sciences City University of Hong Kong Hong Kong China.

Abstract Accurate forecast of future container throughput of a port is very important for its con struction, upgrading, and operation management. This study proposes a transfer forecasting model guided by discrete particle swarm optimization algorithm （TF-DPSO）. It firstly transfers some related time series in source domain to assist in modeling the target time series by transfer learning technique, and then constructs the forecasting model by a pattern matching method called analog complexing. Finally, the discrete particle swarm optimization algorithm is introduced to find the optimal match between the two important parameters in TF-DPSO. The container throughput time series of two im portant ports in China, Shanghai Port and Ningbo Port are used for empirical analysis, and the results show the effectiveness of the proposed model.

关键词： Analog complexing container throughput forecasting discrete particle swarm optimiza-tion transfer forecasting model.

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Three-dimensional carbon framework as high-proportion sulfur host for high-performance lithium-sulfur batteries

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Journal of Materials Science & Technology 2020年第13期47卷 84-91页

作者： Xianbin Liu Zechen xiao Changgan lai Shuai Zou ming Zhang Kaixi Liu Yanhong Yin Tongxiang Liang Ziping Wu School of Materials Science and Engineering Jiangxi University of Science and TechnologyGanzhou 341000China

Lithium-sulfur(Li-S)batteries are anticipated as one of the most promising candidates for the highenergy-density storage ***,the insulating nature and shuttling effect of sulfur severely limits their *** incorporation of sulfur with carbon materials has been deemed as one of the most powerful strategies to improve electrical conductivity and suppress soluble polysulfide ***,a novel three-dimensional carbon framework(3DCF)is prepared and employed as a sulfur host(3DCF@S)for Li-S *** 3DCF not only supplies abundant paths for lithium ion diffusion and electron transport,but also strengthens polysulfide immobilization during the lithium/sulfur conversion *** a result,the 3DCF@S with high sulfur content of 90%exhibits a high capacity of 1366 mA h/g at 0.1 C and excellent cycling stability with a satisfactory capacity of 601 mA h/g after 600 cycles at 2.0 *** resultant Li-S button battery based 3DCF@S electrode could power a light-emitting diode for 2 *** acquired 3DCF@S is expected to be widely used in Li-S batteries and this study will promote developments of carbon/sulfur composites for Li-S batteries.

关键词： 3D carbon framework High sulfur proportion Lithium-sulfur battery High capacity Outstanding cycling stability

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Theoretical Researches on the Charge Transport Properties of Humic Acid Coordinating with Fe^(3+)\Cu^(2+)\Al^(3+) Metal Ions

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Chinese Journal of Structural Chemistry 2019年第3期38卷 439-447页

作者： LUO Hui CHEN xiao LIU Liu-Xie ZHANG ming LI lai-Cai TIAN An-ming College of Chemistry and Material Science Sichuan Normal University Department of Chemistry Sichuan University

Humic acid is a key component of extracellular electron acceptor. Experimental study elucidates that humic acid molecular ligand with different metal elements has different abilities to accept electrons. By using density functional theory, this article selected the leonardite humic acid(LHA) organic macromolecule as ligand to study interactions between the ligand and different metals. At the same time, the calculation of binding energy, the analysis of characteristics for the complex structure and the distribution of frontier molecular orbital were also completed. On the basis of Marcus theory, the reorganization energy, matrix element and charge transport rate constant were calculated. The results show that the order of the charge transfer rates is Fe^(3+)>Cu^(2+)>Al^(3+) for different metal complexes, and are in good agreement with the experimental ones.

关键词： charge transport density functional theory humic acid Marcus theory

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