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p53 coordinates with △133p53 isoform to promote cell survival under low-level oxidative stress

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分子细胞生物学报:英文版 2016年第1期8卷 88-90页

作者： Lu Gong xiao Pan Zhi-min Yuan Jinrong Peng Jun Chen Key laboratory for Molecular Animal Nutrition Ministry of EducationInnovation Center for Signaling NetworkCollege of Life SciencesZhejiang UniversityHangzhou 310058China Department of Genetics and Complex Diseases Harvard School of Public HealthBostonMA 02115USA College of Animal Sciences Zhejiang UniversityHangzhou 310058China

Dear Editor,Reactive oxygen species(ROS)can serve as intracellular signals that promote cell proliferation and survival at sub-toxic levels,or function as toxic substances that cause cell death and senescence at high levels(Weinberg and Chandel,2009).p53 plays a key role in the control of cellular response to ROS by upregulating the expression of either antioxidant genes under low level of oxidative stresses or pro-oxidative and apoptotic genes under high level of stresses(Vigneron and Vousden,2010;Hafsi and Hainaut,2011).However,how p53 differentially regulates gene expression in response to different ROS level remains elusive.△133p53 is an N-terminal truncated form of p53(Bourdon et al.,2005)and functions to antagonize p53 apoptotic activity and to promote DNA double-strand break repair(Chen et al.,2009;Aoubala et al.,2011;Gong et al.,2015).In this study,we investigated the functional interaction between p53 and △133p53 in response to various levels of ROS.

关键词： al. oxidative p53

孕期传统和新兴真菌毒素暴露的胚胎发育毒性及其生物监测的研究现状

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粮油食品科技 2024年第4期32卷 16-23页

作者：韩小敏叶金纪剑徐文静吴宇叶永丽 Rudolf KRSKA 孙秀兰白莉国家食品安全风险评估中心北京100022 国家粮食和物资储备局科学研究院粮油质量安全研究所北京100037 江南大学食品学院江苏无锡214122 维也纳自然资源与生命科学大学生物分析与农业代谢组学研究所农业生物技术系奥地利图尔恩3430 贝尔法斯特女王大学生物科学学院全球食品安全研究所英国北爱尔兰贝尔法斯特BT95DL

真菌毒素是某些真菌菌种在特定条件下产生的有毒次级代谢产物。研究发现多种真菌毒素的联合暴露可引起胎儿发育迟缓、胚胎停育甚至流产等胚胎发育毒性。目前,尚没有关于孕期真菌毒素暴露对宫内胎儿胚胎发育毒性的影响和生物监测情况的... 详细信息

真菌毒素是某些真菌菌种在特定条件下产生的有毒次级代谢产物。研究发现多种真菌毒素的联合暴露可引起胎儿发育迟缓、胚胎停育甚至流产等胚胎发育毒性。目前,尚没有关于孕期真菌毒素暴露对宫内胎儿胚胎发育毒性的影响和生物监测情况的系统报道。因此,为预防和降低孕期真菌毒素暴露带来的危害,本文梳理了孕期传统真菌毒素如黄曲霉毒素、脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮和新兴真菌毒素如交链孢毒素、白僵菌素和恩镰孢菌素等暴露引起的胚胎发育毒性,以及以生物标志物如暴露标志物和效应标志物为基础的生物监测技术的研究现状。

关键词：孕期传统和新兴真菌毒素胚胎发育毒性生物监测

Disinfection and Protective Measures for Makeshift Hospitals

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Biomedical and Environmental Sciences 2020年第12期33卷 940-942页

作者： WANG Jia Qi LIU xiao Li DUAN Hong Yang CHEN xiao min QIAN Le LYU Xi Fang CHENG Yi Bin LIANG Jian Sheng YAO xiao Yuan National Institute of Environmental Health Chinese Center for Disease Control and PreventionBeijing 100021China Wuhan Center For Disease Control and Prevention Wuhan 430024HubeiChina

During the COVID-19 epidemic,Wuhan city had the hardest hit in *** treatment capacity of the local medical system exceeded due to the rapid growth of COVID-19 *** a result,a considerable part of diagnosed patients could not be admitted to hospitals for isolation and treatment in time,causing the risk of spreading in the *** effectively achieve the goals of controlling the source of infection by cutting off the transmission route,increasing the cure rate and reducing the mortality rate,Wuhan has reconstructed several makeshift hospitals based on large public places.

关键词： China. admitted Wuhan

Peripheral zone PSA density:a predominant variable to improve prostate cancer detection efficiency in men with PSA higher than 4 ng ml^(-1)

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Asian Journal of Andrology 2021年第4期23卷 415-420页

作者： Cheng Wang Yue-Yang Wang shi-Yuan Wang Ji-Xiang Ding Mao Ding Yuan Ruan xiao-Hai Wang Yi-Feng Jing Bang-min Han Shu-Jie Xia Chen-Yi Jiang Fu-Jun Zhao Department of Urology Jiangsu Jiangyin People's HospitalJiangyin 214400China Department of Urology Shanghai General Hospital of Nanjing Medical UniversityShanghai 200080China Department of Urology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 200080China Department of Urology Kashgar Prefecture Second People's HospitalKashi 844000China

To improve the diagnostic efficiency of prostate cancer(PCa)and reduce unnecessary biopsies,we defined and analyzed the diagnostic efficiency of peripheral zone prostate-specific antigen(PSA)density(PZ-PSAD).Patients who underwent systematic 12-core prostate biopsies in Shanghai General Hospital(Shanghai,China)between January 2012 and January 2018 were retrospectively identified(n=529).Another group of patients with benign prostatic hyperplasia(n=100)were randomly preselected to obtain the PSA density of the non-PCa cohort(N-PSAD).Prostate volumes and transition zone volumes were measured using multiparameter magnetic resonance imaging(mpMRI)and were combined with PSA and N-PSAD to obtain the PZ-PSAD from a specific *** operating characteristic(ROC)curve analysis was used to assess the PCa detection efficiency in patients stratified by PSA level,and the area under the ROC curve(AUC)of PZ-PSAD was higher than that of PSA,PSA density(PSAD),and transition zone PSA density(TZ-PSAD).PZ-PSAD could amend the diagnosis for more than half of the patients with inaccurate transrectal ultrasonography(TRUS)and mpMRI *** TRUS and mpMRI findings were ambiguous to predict PCa(PIRADS score≤3),PZ-PSAD could increase the positive rate of biopsy from 21.7%to 54.7%,and help 63.8%(150/235)of patients avoid unnecessary prostate *** patients whose PSA was 4.0–10.0 ng ml^(−1),10.1–20.0 ng ml^(−1),and>20.0 ng ml^(−1),the ideal PZ-PSAD cut-off value for predicting clinically significant PCa was 0.019 ng ml^(−2),0.297 ng ml^(−2),and 1.180 ng ml^(−2),respectively(sensitivity>90%).Compared with PSA,PSAD,and TZ-PSAD,the efficiency of PZ-PSAD for predicting PCa is the highest,leading to fewer missed diagnoses and unnecessary biopsies.

关键词： benign prostate hyperplasia detection efficiency peripheral zone prostate-specific antigen density prostate biopsy prostate-specific antigen prostatic neoplasm

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复方布洛肾素那敏片的开发及体外溶出评估

生物化工 2024年第1期10卷 18-23页

作者：郭铠溶杜欢欢尹民施斌贺敦伟中国海洋大学医药学院山东青岛266003 上海则正医药科技股份有限公司上海201807

目的:制备复方布洛肾素那敏片,优化处方组成与制备工艺,评价自制制剂和参比制剂溶出曲线的相似性。方法:将布洛芬与马来酸氯苯那敏组合进行湿法制粒,盐酸去氧肾上腺素单独进行湿法制粒,考察崩解剂交联羧甲基纤维素钠、粘合剂羟丙甲纤维... 详细信息

目的:制备复方布洛肾素那敏片,优化处方组成与制备工艺,评价自制制剂和参比制剂溶出曲线的相似性。方法:将布洛芬与马来酸氯苯那敏组合进行湿法制粒,盐酸去氧肾上腺素单独进行湿法制粒,考察崩解剂交联羧甲基纤维素钠、粘合剂羟丙甲纤维素、润滑剂硬脂酸镁用量,以及布洛芬原料粒径等影响因素对溶出的影响,确定最终处方组成。结果:经过处方筛选,在片剂总重480 mg条件下,确定布洛芬原料粒径范围为170μm≤D90≤190μm,崩解剂交联羧甲基纤维素钠用量为25.2 mg,粘合剂羟丙甲纤维素用量为4.2 mg,润滑剂硬脂酸镁用量为4.2 mg,自制制剂与参比制剂在4种溶出介质中溶出曲线的相似因子(f2)值高于50。结论:成功制备复方布洛肾素那敏片,制剂处方简单,工艺稳定,重现性好,溶出曲线与参比制剂相似,为后期生产布洛肾素那敏片剂提供理论依据。

关键词：布洛肾素那敏片制备工艺体外溶出一致性

Differentiation of human amniotic epithelial cells into corneal epithelial-like cells in vitro

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International Journal of Ophthalmology(English edition) 2013年第5期6卷 564-572页

作者： min Yao Jian Chen xiao-Xi Yang xiao-Ling Zhang Qing-Shan Ji Qing Zhou Jin-Tang Xu Department of Ophthalmology the First Affiliated Hospital of Jinan University Department of Ophthalmology the Sixth Affiliated Hospital of Sun Yat-sen University

·AIM: To explore the feasibility that human amniotic epithelial cells (hAECs) have the potential to differentiate into corneal epithelial -like cells under the microenvironment replicated by spontaneously immortalized human corneal epithelial cells (S-ihCECs). ·METHODS: hAECs were isolated by enzyme digestion, and flow cytometry was used to analysis the expression of CD29/90/166/73/34 and HLA -DR. Recovered and cultured S -ihCECs, immunocytochemistry was used to detect the expression of CK3/12. The proliferation of S - ihCECs handled by different concentrations of mitomycin was detected by CCK -8. The proliferation of hAECs cultured by S-ihCECs culture media collected at different time was analyzed by CCK -8. After filtered out the optimal conditions, we collected S-ihCECs culture media for 5 days, then prepared conditioned medium to incubate hAECs, inverted phase contrast microscope and scanning electron microscope were used to observe the change of morphology in hAECs. Quantitative real -time reverse transcription -polymerase chain reaction (QRT - PCR) was carried out to evaluate the expression of Oct - 4, NANOG, PAX6, and CK12 in the differentiation period. Immunocytochemistry and western bloting were used to detect the expression of CK3/12. ·RESULTS: The culture media collected every 12h, from 20μg/mL mitomycin pretreatment S -ihCECs could significantly promote the proliferation of hAECs. In the period of differentiation, the morphology of differentiated hAECs was obviously different compared with the control group, and the distinctive CK3/12 for corneal epithelial cells was detected.·CONCLUSION: This study showed that hAECs can differentiate into corneal epithelial -like cells by replication of the corneal epithelial microenvironment, using the culture media collected from S -ihCECs, and it is possible that S -ihCECs culture media could be used in corneal tissue engineering. ·

关键词： human amniotic epithelial cells spontaneously immortalized human corneal epithelial cells mytomicin microenvironment tissue engineering

TiO_(2)光催化降解磺胺类抗生素的研究进展

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广州化工 2024年第16期52卷 14-16页

作者：郭俊材张孝琴王译伟刘小凤郭建敏西南医科大学基础医学院四川泸州646000

磺胺类抗生素滥用引发的环境问题日益严重,而光催化是目前最有前途的处理方法之一。这篇文章归纳了以单一TiO_(2)作为催化材料光催化降解磺胺类抗生素的主要问题,综述了TiO_(2)光催化降解磺胺类抗生素的机理和TiO_(2)光催化剂的改性方法... 详细信息

磺胺类抗生素滥用引发的环境问题日益严重,而光催化是目前最有前途的处理方法之一。这篇文章归纳了以单一TiO_(2)作为催化材料光催化降解磺胺类抗生素的主要问题,综述了TiO_(2)光催化降解磺胺类抗生素的机理和TiO_(2)光催化剂的改性方法,包括石墨烯基TiO_(2)复合改性,超声协同二氧化钛光催化法,重点介绍了TiO_(2)掺杂金属或非金属成分的改性方法。为以TiO_(2)为主要催化剂的光催化降解磺胺类抗生素方法提供借鉴。

关键词： TiO_(2)光催化磺胺类抗生素降解

昆虫病原线虫对红火蚁的致病力及田间防控效果

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环境昆虫学报 2024年第3期46卷 711-717页

作者：易松望李晓维陈利民王金超黄俊张娟吕要斌浙江农林大学现代农学院杭州311300 浙江省农业科学院植物保护与微生物研究所杭州310021 浙江省园林植物与花卉研究所杭州311122

红火蚁Solenopsis invicta Buren是一种国际重大外来入侵生物,严重危害农林生产、生命健康、公共安全和生态环境。本研究在室内条件下测定了小卷蛾斯氏线虫Steinernema carpocapsae All品系、长尾斯氏线虫*** X-7品系和芫菁夜蛾斯氏线虫... 详细信息

红火蚁Solenopsis invicta Buren是一种国际重大外来入侵生物,严重危害农林生产、生命健康、公共安全和生态环境。本研究在室内条件下测定了小卷蛾斯氏线虫Steinernema carpocapsae All品系、长尾斯氏线虫*** X-7品系和芫菁夜蛾斯氏线虫*** SN品系对红火蚁的致病力,并筛选出高致病力品系及其对红火蚁的田间防控效果。结果表明,不同昆虫病原线虫对红火蚁不同虫态的致病力存在显著差异。3种线虫对红火蚁幼虫具有较高的致病力,处理3 d后,幼虫的累计校正致死率均为100%。3种线虫对工蚁的致病力均较弱,处理10 d后的校正死亡率均小于30%。与芫菁夜蛾斯氏线虫SN品系相比,长尾斯氏线虫X-7品系和小卷蛾斯氏线虫All品系对蛹和生殖蚁具有较强的致病能力,10 d后累计校正死亡率均达到90%以上。田间试验表明,施用小卷蛾斯氏线虫All品系对红火蚁工蚁的防控效果最高可达63.7%,且不会增加红火蚁的迁巢风险。综上所述,小卷蛾斯氏线虫All品系和长尾斯氏线虫X-7品系为红火蚁的高致病品系,田间施用小卷蛾斯氏线虫All品系对红火蚁工蚁具有显著的控制作用,可推荐用于红火蚁的生物防治。

关键词：红火蚁昆虫病原线虫致病力田间防控效果

Oncogenic Role of Skp2 and p27^(Kip1) in Intraductal Proliferative Lesions of the Breast

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Chinese Medical Sciences Journal 2012年第3期27卷 161-166页

作者： Yan Lv Yun Niu Xiu-min Ding Xu-qi xiao Department of Acupuncture and Moxibustion Tianjin University of Traditional Chinese MedicineTianjin 300193China Breast Cancer Research and Pathology Laboratory Cancer Institute&Hospital of Tianjin Medical UniversityTianjin 300060China

Objective To investigate whether the connection of p27 Kip1 to S-phase kinase-associated protein 2 (Skp2) plays an oncogenic role in intraductal proliferative lesions of the breast. Methods Here we investigated the mechanism involved in association of Skp2's degradation of p27 Kip1 with the breast carcinogenesis by immunohistochemical method through detection of Skp2 and p27 Kip1 protein levels in 120 paraffin-embedded tissues of intraductal proliferative lesions including usual ductal hyperplasia (UDH, n=30), atypical ductal hyperplasia (n=30), flat epithelial atypia (FEA, n=30), and ductal carcinoma in situ (DCIS, n=30). Moreover, the expression status of Skp2 and p27 Kip1 in 30 cases of the normal breast paraffin-embedded tissues were explored. Results The DCIS group was with the highest Skp2 level and the lowest p27 Kip1 level, and the UDH group was with the lowest Skp2 level and the highest p27 Kip1 level. Both Skp2 and p27 Kip1 levels in the DCIS group were significantly different from those in the UDH group (all P<0.01). The levels of Skp2 and p27 Kip1 in the FEA group were significantly different from both the DCIS and UDH groups (all P<0.05). p27 Kip1 was negatively correlated with Skp2 in both the UDH group (r=-0.629, P=0.026) and DCIS group (r=-0.893, P=0.000). Conclusion Overexpression of Skp2 might be the mechanism underlying p27 Kip1 over degradation.

关键词： breast cancer intraductal proliferative lesions p27 ^Kip1 S-phase kinase-associated protein 2