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Aerobic degradation of methyl tert-butyl ether by a Proteobacteria strain in a closed culture system

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Journal of Environmental Sciences 2007年第1期19卷 18-22页

作者： ZHONG Wei-hong CHEN Jian-meng lu Zheng CHEN Dong-zhi CHEN xiao College of Biological and Environmental Engineering Zhefiang University of Technology Hangzhou 310032 China

The contamination of methyl ten-butyl ether （MTBE） in underground waters has become a widely concerned problem all over the world. In this study, a novel dosed culture system with oxygen supplied by H2O2 was introduced for MTBE aerobic biodegradation. After 7 d, almost all MTBE was degraded by a pure culture, a member of β-Proteobacteria named as PMI, in a closed system with oxygen supply, while only 40% MTBE was degraded in one without oxygen supply. Dissolved oxygen （DO） levels of the broth in closed systems respectively with and without H2O2 were about 5-6 and 4 mg/L. Higher DO may improve the activity of monooxygemase, which is the key enzyme of metabolic pathway from MTBE to tert-butyl alcohol and finally to CO2, and may result in the increase of the degrading activity of PM1 cell. The purge and trap GC-MS result of the broth in closed systems showed that tea-butyl alcohol, isopronol and acetone were the main intermediate products.

关键词： methyl tert-butyl ether biodegradation β-Proteobacteria intermediate products

Thermoresponsive helical dendronized poly(arylacetylene)s:modulating the dynamic chirality

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Science China Chemistry 2025年

作者： Xueting lu Lei Wang Liangxuan Ren Wen Li Afang Zhang International Joint Laboratory of Biomimetic&Smart Polymers School of Materials Science and EngineeringShanghai University

Helical poly(arylacetylene)s have formed an intriguing class of polymers,whose chiralities are dynamic and liable to external *** combining dendronized polymer topology with dendritic oligoethylene glycols (OEGs),we here report on synthesis of a homologous series of thermoresponsive dendronized poly(arylacetylene)s with either poly(phenyl acetylene) or poly(naphthalene acetylene) as the backbones,and investigate tunability of their helical *** polymers carry dendritic OEG pendants with either methoxyl-or ethoxyl-terminals to tune their unprecedent thermoresponsive behaviors,and at the same time,dendritic shielding plays an important role in mediating their thermal phase transition *** of these polymers is originated from the chiral alanine or phenylalanine moieties within the dendritic pendants,which can be tailored through manipulating solvation in different organic solvents or via thermal dehydration and collapse in ***,achiral additives such as linear or dendritic OEGs and benzene derivatives can act similarly as thermal dehydration to induce chirality transitions of these polymers in aqueous phase through interplay competitions between the additives and the polymers against their hydration.

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Inhibiting ceramide synthase 5 expression in microglia decreases neuroinflammation after spinal cord injury

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Neural Regeneration Research 2025年第10期20卷 2955-2968页

作者： Wei Zhang Yubao lu Ruoqi Shen Yingjie Wu Chenrui Liu Xingxing Fang Liangming Zhang Bin Liu Limin Rong Department of Spine Surgery The Third Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina National Medical Products Administration(NMPA)Key Laboratory for Quality Research and Evaluation of Cell Products GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery GuangzhouGuangdong ProvinceChina

Microglia,the resident monocyte of the central nervous system,play a crucial role in the response to spinal cord ***,the precise mechanism remains *** investigate the molecular mechanisms by which microglia regulate the neuroinflammatory response to spinal cord injury,we performed single-cell RNA sequencing dataset analysis,focusing on changes in microglial *** found that the MG1 subpopulation emerged in the acute/subacute phase of spinal cord injury and expressed genes related to cell pyroptosis,sphingomyelin metabolism,and neuroinflammation at high ***,we established a mouse model of contusive injury and performed intrathecal injection of siRNA and molecular inhibitors to validate the role of ceramide synthase 5 in the neuroinflammatory responses and pyroptosis after spinal cord ***,we established a PC12-BV2 cell co-culture system and found that ceramide synthase 5 and pyroptosis-associated proteins were highly expressed to induce the apoptosis of neuron *** ceramide synthase 5 expression in a mouse model of spinal cord injury effectively reduced ***,ceramide synthase 5-induced pyroptosis was dependent on activation of the NLRP3 signaling *** ceramide synthase 5 expression in microglia in vivo reduced neuronal apoptosis and promoted recovery of neurological ***2g7 formed a“bridge”between sphingolipid metabolism and ceramide synthase 5-mediated cell death by inhibiting the NLRP3 signaling ***,these findings suggest that inhibiting ceramide synthase 5 expression in microglia after spinal cord injury effectively suppressed microglial pyroptosis mediated by NLRP3,thereby exerting neuroprotective effects.

关键词： ceramide synthase 5 gasdermin D microglia neuroinflammation NLRP3 nuclear factor kappa B Pla2g7 pyroptosis sphingomyelin metabolism spinal cord injury

Analysis of RNA-Dependent RNA Polymerase Sequence of Infectious Flacherie Virus Isolated in China and Its Expression in BmN Cells

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Agricultural Sciences in China 2009年第7期8卷 872-879页

作者： LI Ming-qian lu Qi-neng WU xiao-Feng MAN Na-na CHEN xiao-xue JIN Wei lu Xing-meng Laboratory of lnvertebrate Pathology College of Animal Sciences Zhejiang University Hangzhou 310029 P.R.China

Full gene sequence of RNA-dependent RNA polymerase （RdRp） from Bombyx mori infectious flacherie virus isolated in Zhejiang Province, China （Zhejiang01/CHN/2002） was cloned. The sequence was 1 920 nucleotides in length coding 639 amino acid residues. Sequences comparison of RdRp showed Zhejiang01/CHN/2002 was 99.7% nucleotide sequence and 99.1% amino acids sequence homology with Japanese strain. The RdRp sequence was aligned with 8 representative picorna（-like） viruses and 8 highly conserved regions were detected. The result indicated their relevance function. Phylogenetic tree of 14 picorna（-like） viruses which RdRp presumed protein sequences revealed that the viruses from Iflavirus genus formed an independent clade. The RdRp was successfully expressed in BmN cells using Bac-to-Bac expression system.

关键词： infectious flacherie virus RNA-dependent RNA polymerase phylogenetic analysis BmN cells expression

Protein arginine methyltransferase-6 regulates heterogeneous nuclear ribonucleoprotein-F expression and is a potential target for the treatment of neuropathic pain

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Neural Regeneration Research 2025年第9期20卷 2682-2696页

作者： xiaoyu Zhang Yuqi Liu Fangxia Xu Chengcheng Zhou Kaimei lu Bin Fang Lijuan Wang Lina Huang Zifeng Xu Department of Anesthesiology International Peace Maternity and Child Health HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Anesthesiology Shanghai General HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Shanghai Key Laboratory of Embryo Original Diseases ShanghaiChina

Protein arginine methyltransferase-6 participates in a range of biological functions,particularly RNA processing,transcription,chromatin remodeling,and endosomal ***,it remains unclear whether protein arginine methyl transferase-6 modifies neuropathic pain and,if so,what the mechanisms of this *** this study,protein arginine methyltransferase-6 expression levels and its effect on neuropathic pain were investigated in the spared nerve injury model,chronic constriction injury model and bone cancer pain model,using immunohistochemistry,western blotting,immunoprecipitation,and label-free proteomic *** results showed that protein arginine methyltransferase-6 mostly co-localized withβ-tubulinⅢin the dorsal root ganglion,and that its expression decreased following spared nerve injury,chronic constriction injury and bone cancer *** addition,PRMT6 knockout(Prmt6~(-/-))mice exhibited pain ***,the development of spared nerve injury-induced hypersensitivity to mechanical pain was attenuated by blocking the decrease in protein arginine methyltransferase-6 ***,when protein arginine methyltransferase-6 expression was downregulated in the dorsal root ganglion in mice without spared nerve injury,increased levels of phosphorylated extracellular signal-regulated kinases were observed in the ipsilateral dorsal horn,and the response to mechanical stimuli was ***,protein arginine methyltransferase-6 appeared to contribute to spared nerve injury-induced neuropathic pain by regulating the expression of heterogeneous nuclear ***,protein arginine methyltransfe rase-6-mediated modulation of hete rogeneous nuclear ribonucleoprotein-F expression required amino atids 319 to 388,but not classical H3R2 *** findings indicated that protein arginine methyltransferase-6 is a potential therapeutic target fo r the treatment of peripheral neuro pathic pain.

关键词： dorsal root ganglion heterogeneous nuclear ribonucleoprotein F neuropathic pain protein arginine methyltransferase-6 sensory neurons

Research on the effect of the heavy nuclei amount on the temperature reactivity coefficient in a small modular molten salt reactor

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Nuclear Science and Techniques 2019年第9期30卷 83-93页

作者： meng-lu Tan Gui-Feng Zhu Yang Zou xiao-Han Yu Ye Dai Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China University of Chinese Academy of Sciences Beijing 100049 China

Small modular thorium-based graphite-moderated molten salt reactors (smTMSRs), which combine the advantages of small modular reactors and molten salt reactors, are regarded as a wise development path to speed deployment time. In a smTMSR, low enriched uranium and thorium fuels are used in once-through mode, which makes a marked difference in their neutronic properties compared with the case when a conventional molten salt breeder reactor is used. This study investigated the temperature reactivity coefficient (TRC) in a smTMSR, which is mainly affected by the molten salt volume fraction (VF) and the heavy nuclei concentration in the fuel salt (HN). The fourfactor formula method and the reaction rate method were used to indicate the reasons for the TRC change, including the fuel density effect, the fuel Doppler effect, and the graphite thermal scattering effect. The results indicate that only the fuel density has a positive effect on the TRC in the undermoderated region. Thermal scattering from both salt and graphite has a significant negative influence on the TRC in the overmoderated region. The maximal effective multiplication factor, which shows the highest fuel utilization, is located at 10% VF and 12 mol% HN and is still located in the negative TRC region. In addition, on increasing the heavy nuclei amount from 2 mol% HN to 12 mol% HN (VF = 10%), the total TRC undergoes an obvious change from - 11 to - 3 pcm/K, which implies that the change in the HN caused by the fuel feed online should be small to avoid potential trouble in the reactivity control scheme.

关键词： Molten salt reactor Temperature reactivity coefficient Heavy nuclei amount

A Finite-Time Recurrent Neural Network for Computing Quadratic Minimization with Time-Varying Coefficients

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Chinese Journal of Electronics 2019年第2期28卷 253-258页

作者： xiao Lin lu Rongbo College of Information Science and Engineering Jishou University

This paper proposes a Finite-time Zhang neural network(FTZNN) to solve time-varying quadratic minimization problems. Different from the original Zhang neural network(ZNN) that is specially designed to solve time-varying problems and possesses an exponential convergence property, the proposed neural network exploits a sign-bi-power activation function so that it can achieve the finite-time convergence. In addition, the upper bound of the finite convergence time for the FTZNN model is analytically estimated in theory. For comparative purposes, the original ZNN model is also presented to solve time-varying quadratic minimization problems. Numerical experiments are performed to evaluate and compare the performance of the original ZNN model and the FTZNN model. The results demonstrate that the FTZNN model is a more effective solution model for solving time-varying quadratic minimization problems.

关键词： Recurrent neural network Quadratic minimization Activation function Finite-time convergence Comparative verification

Design and evaluation of a novel biopsy needle with hemostatic function

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Frontiers of Mechanical Engineering 2023年第2期18卷 319-331页

作者： xiaolong ZHU Yichi MA xiao xiao Liang lu Wei xiao Ziqi ZHAO Hongliang REN Max Q.-H.meng Department of Electronic and Electrical Engineering Southern University of Science and TechnologyShenzhen 518055China Department of Mechanical Engineering University of CaliforniaBerkeleyBerkeleyCA 48072USA Department of Electronic Engineering The Chinese University of Hong KongHong KongChina

Biopsy is a method commonly used for early cancer ***,bleeding complications of widely available biopsy are risky for *** biopsy will result in a more accurate cancer diagnosis and a decrease in the risk of *** this article,we propose a novel biopsy needle that can reduce bleeding during biopsy procedures and achieve stable *** proposed biopsy needle features a compact structure and can be operated easily by left and right hands.A predictive model for puncture force and tip deflection based on coupled Eulerian–Lagrangian(CEL)method is *** results show that the biopsy needle can smoothly deliver the gelatin sponge hemostatic plug into the *** the hemostatic plug bends,the overall delivery process is stable,and the hemostatic plug retains in the tissue without being affected by the withdrawal of the *** experiments indicate that the specimens are well obtained and evenly distributed in the groove of the outer needle without *** proposed design of biopsy needle possesses strong ability of hemostasis,tissue cutting,and tissue *** CEL model accurately predicts the peak of puncture force and produces close estimation of the insertion force at the postpuncture stage and tip position.

关键词： cancer diagnosis biopsy needle hemostatic function predictive model coupled Eulerian−Lagrangian

On Sums of a Prime and Four Prime Squares in Short Intervals

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Acta Mathematica Sinica,English Series 2008年第8期24卷 1291-1302页

作者： Guang Shi lu Xian meng meng Department of Mathematics Shandong University Ji'nan 250100 P. R. China Department of Statistics and Mathematics Shandong Finance Institute Ji'nan 250014 P. R. China

In this paper, we prove that each sufficiently large integer N ≠1（mod 3） can be written as N=p＋p1^2＋p2^2＋p3^2＋p4^2, with｜p-N/5｜≤U,｜pj-√N/5｜≤U,j=1,2,3,4,where U=N^2/20＋c and p,pj are primes.

关键词： exponential sums over primes circle method prime