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Neural Regeneration Research 2015年第10期10卷 1635-1642页

作者： Ya-ling Wang xiao-mei gu Yan Kong Qi-lin Feng Yu-min Yang Key Laboratory of Eco-Textiles Ministry of Education Jiangnan University School of Chemistry and Chemical Engineering Nantong University Jiangsu College of Engineering and Technology Jiangsu Key Laboratory of Neuroregeneration Nantong University School of Medicine Nantong University

We have designed a novel nerve guidance conduit（NGC） made from silk fibroin and poly（lactic-co-glycolic acid） through electrospinning and weaving（ESP-NGCs）. Several physical and biological properties of the ESP-NGCs were assessed in order to evaluate their biocompatibility. The physical properties, including thickness, tensile stiffness, infrared spectroscopy, porosity, and water absorption were determined in vitro. To assess the biological properties, Schwann cells were cultured in ESP-NGC extracts and were assessed by morphological observation, the MTT assay, and immunohistochemistry. In addition, ESP-NGCs were subcutaneously implanted in the backs of rabbits to evaluate their biocompatibility in vivo. The results showed that ESP-NGCs have high porosity, strong hydrophilicity, and strong tensile stiffness. Schwann cells cultured in the ESP-NGC extract fluids showed no significant differences compared to control cells in their morphology or viability. Histological evaluation of the ESP-NGCs implanted in vivo indicated a mild inflammatory reaction and high biocompatibility. Together, these data suggest that these novel ESP-NGCs are biocompatible, and may thus provide a reliable scaffold for peripheral nerve repair in clinical application.

关键词： nerve regeneration peripheral nerve injury poly(lactic-co-glycolic acid) electrospinning silk fibroin biocompatibility nerve guidance conduit weaving

Adeno-associated virus-mediated Bcl-XL prevents aminoglycosideinduced hearing loss in mice

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Chinese Medical Journal 2007年第14期120卷 1236-1240页

作者： LIU Yu-he KE xiao-mei QIN Yong gu Zhi-ping xiao Shui-fang Department of Otolaryngology Head and Neck Surgery Peking University First Hospital Beijing 100034 China

Background Recent studies showed that aminoglycosides destroyed the cochlear cells and induced ototoxicity by producing reactive oxygen species, including free radicals in the mitochondria, damaging the membrane of mitochondria and resulting in apoptotic cell death. Bcl-XL is a well characterized anti-apoptotic member of the Bcl-2 family. The aim of this study was to determine the potential cochlear protective effect of Bcl-XL as a therapeutic agent in the murine model of aminoglycoside ototoxicity. Methods Serotype 2 of adeno-associated virus （AAV2） as a vector encoding the mouse Bcl-XL gene was injected into mice cochleae prior to injection of kanamycin. Bcl-XL expression in vitro and in vivo was examined with Western blotting and immunohistochemistry separately. Cochlear dissection and auditory steady state responses were checked to evaluate the cochlear structure and function. Results The animals in the AAV2-Bcl-XL/kanamycin group displayed better auditory steady state responses hearing thresholds and cochlear structure than those in the artificial perilymph/kanamycin or AAV2-enhanced humanized green fluorescent protein/kanamycin control group at all tested frequencies. The auditory steady state responses hearing thresholds and cochlear structure in the inoculated side were better than that in the contralateral side. Conclusions AAV2-Bcl-XL afforded significant preservation of the cochlear hair cells against ototoxic insults and protected the cochlear function. AAV2-mediated BCI-XL might be an approach with respect to potential therapeutic application in the cochlear degeneration.

关键词： vector, adeno-associated virus gene therapy apoptosis hearing loss

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基于鲁棒主成分分析的红外图像小目标检测

兵工学报 2016年第9期37卷 1753-1760页

作者：王忠美杨晓梅顾行发电子科技大学自动化工程学院四川成都610054 中国科学院地理与资源研究所北京100101 中国科学院遥感与数字地球研究所北京100101

鲁棒的小目标检测是红外目标搜索与跟踪的关键技术,提出一种改进的单帧红外图像小目标检测算法。该方法将原始红外图像通过预处理变换到新的红外块图像模式,在红外块图像上,将红外图像小目标检测问题转换为低秩矩阵和稀疏矩阵分离的鲁... 详细信息

鲁棒的小目标检测是红外目标搜索与跟踪的关键技术,提出一种改进的单帧红外图像小目标检测算法。该方法将原始红外图像通过预处理变换到新的红外块图像模式,在红外块图像上,将红外图像小目标检测问题转换为低秩矩阵和稀疏矩阵分离的鲁棒主成分分析(RPCA)问题。考虑到红外图像中噪声和杂波的存在,用交替方向方法求解带噪声的RPCA问题,获得稀疏目标图像,并对获得的稀疏目标图像采用简单的图像分割算法进行目标检测。对空天、海天、天云、海面4种不同场景的红外图像小目标检测,进行仿真实验,结果验证了所提出算法的有效性。

关键词：兵器科学与技术红外图像小目标检测块图像模型低秩矩阵恢复鲁棒主成分分析

Huangqin decoction alleviates lipid metabolism disorders and insulin resistance in nonalcoholic fatty liver disease by triggering Sirt1/NF-κB pathway

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World Journal of Gastroenterology 2023年第31期29卷 4744-4762页

作者： Bao-Fei Yan Lan-Fen Pan Yi-Fang Quan Qian Sha Jing-Zheng Zhang Yi-Feng Zhang Li-Bing Zhou Xi-Long Qian xiao-mei gu Feng-Tao Li Ting Wang Jia Liu Xian Zheng College of Pharmacy Jiangsu Health Vocational CollegeNanjing 211800Jiangsu ProvinceChina Department of Pathology Affiliated Kunshan Hospital of Jiangsu UniversityKunshan 215300Jiangsu ProvinceChina Department of Education and Science The First People's Hospital of TaicangKunshan 215400Jiangsu ProvinceChina Department of Pharmacy Affiliated Hospital of Xuzhou Medical UniversityXuzhou 221006Jiangsu ProvinceChina School of Pharmacy Nantong UniversityNantong 226019Jiangsu ProvinceChina Department of Pharmacy Affiliated Kunshan Hospital of Jiangsu UniversityKunshan 215300Jiangsu ProvinceChina School of Pharmacy Nanjing University of Chinese MedicineNanjing 210023Jiangsu ProvinceChina

BACKGROUND Nonalcoholic fatty liver disease(NAFLD)is a clinicopathological entity characterized by intrahepatic ectopic *** a consequence of increased consumption of high-calorie diet and adoption of a sedentary lifestyle,the incidence of NAFLD has surpassed that of viral hepatitis,making it the most common cause of chronic liver disease *** decoction(HQD),a Chinese medicinal formulation that has been used clinically for thousands of years,has beneficial outcomes in patients with liver diseases,including ***,the role and mechanism of action of HQD in lipid metabolism disorders and insulin resistance in NAFLD remain poorly *** To evaluate the ameliorative effects of HQD in NAFLD,with a focus on lipid metabolism and insulin resistance,and to elucidate the underlying mechanism of *** High-fat diet-induced NAFLD rats and palmitic acid(PA)-stimulated HepG2 cells were used to investigate the effects of HQD and identify its potential mechanism of *** in HQD were analyzed by highperformance liquid chromatography(HPLC)to identify the key *** Ten primary chemical components of HQD were identified by HPLC *** vivo,HQD effectively prevented rats from gaining body and liver weight,improved the liver index,ameliorated hepatic histological aberrations,decreased transaminase and lipid profile disorders,and reduced the levels of pro-inflammatory factors and insulin *** vitro studies revealed that HQD effectively alleviated PA-induced lipid accumulation,inflammation,and insulin resistance in HepG2 ***-depth investigation revealed that HQD triggers Sirt1/NF-κB pathwaymodulated lipogenesis and inflammation,contributing to its beneficial actions,which was further corroborated by the addition of the Sirt1 antagonist EX-527 that compromised the favorable effects of *** In summary,our study confirmed that HQD mitigates lipid metabolism disorders and insulin resistance i

关键词： Nonalcoholic fatty liver disease Huangqin decoction Lipid metabolism disorders Insulin resistance Sirt1/NF-κB pathway

Design of a cationic accelerator enabling ultrafast ion diffusion kinetics in aqueous zinc-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 377-384页

作者： Yawei xiao Qianqian gu Haoyu Li Mengyao Li Yude Wang National Center for International Research on Photoelectric and Energy Materials School of Materials and Energy Yunnan University Key Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources School of Chemistry and Chemical Engineering Hainan University College of Chemical Engineering Fuzhou University Yunnan Key Laboratory of Carbon Neutrality and Green Low-carbon Technologies Yunnan University

Aqueous zinc-ion batteries are highly favored for grid-level energy storage owing to their low cost and high safety,but their practical application is limited by slow ion *** address this,a strategy has been developed to create a cation-accelerating electric field on the surface of the cathode to achieve ultrafast Zn2+diffusion *** employing electrodeposition to coat MoS2on the surface of BaV6O16·3H2O nanowires,the directional built-in electric field generated at the heterointerface acts as a cation accelerator,continuously accelerating Zn2+diffusion into the active *** optimized Zn2+diffusion coefficient in CC@BaV-6O16·3H2O@MoS2(7.5×10～8cm2s-1) surpasses that of most reported V-based ***,MoS2serving as a cathodic armor extends the cycling life of the Zn-CC@BaV6O16·3H2O@MoS2full batteries to over 10000 *** work provides valuable insights into optimizing ion diffusion kinetics for high-performance energy storage devices.

关键词： Internal electric field Cationic accelerator Ion diffusion kinetics Cathode modification strategy Aqueous zinc ion battery

Plasma homocysteine and major adverse cardiac events in coronary artery disease patients with late hypoglycemia

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Plasma homocysteine and major adverse cardiac events in coro...

Single-cell omics: experimental workflow, data analyses and applications

第十三次全国心血管病学术会议

作者： LI Ning,DING Yue-you,MIAO Peizhi,ZHENG Hong-chao, CUI Ke-jian,HUANG Bo,BAO xiao-mei,gu Shui-ming Xuhui Central Hospital,Shanghai 200031,China

Objective To evaluate a possible relationship between HCY levels and its prognostic value in coronary artery disease (CAD) patients with late hypoglycemia *** Ninety-five CAD patients with late hypoglycemia events were recruited and underwent coronary angiography and in some cases myocardial *** primary end point was the

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei gu Xin Shao Qinqin Wang Xin Dong Bin Duan Feiyang Xing Jun Wu Minmin xiao Fangqing Zhao Jing-Dong J.Han Qi Liu xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

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生物酶在染整加工中的应用及其发展

染整技术 2007年第1期29卷 13-17页

作者：李淑华顾晓梅邱红娟南通纺织职业技术学院江苏南通226007 中国纺织出版社北京100027

介绍了生物酶技术在棉、麻纤维的前处理和后整理、羊毛的防毡缩、真丝的脱胶和砂洗等染整加工中的应用,并讨论了生物酶技术将不断朝着复合化、协同作用、转基因技术等方向发展的趋势。

关键词：生物酶染整前处理后整理发展方向

High efficiency hydrogen purification through P2C3 membrane:A theoretical study

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Chinese Physics B 2019年第12期28卷 389-392页

作者： Zhao-Qin Chu xiao gu Xiang-mei Duan Department of Physics Ningbo UniversityNingbo 315211China Laboratory of Clean Energy Storage and Conversion Ningbo UniversityNingbo 315211China

It is critical to design an effective two-dimensional membrane for hydrogen purification from the mixed gas, due to its wide range of scientific and industrial applications. In this work, we investigate the hydrogen separation performance of P2C3 membranes by density functional theory and molecular dynamics simulations. The results show that the energy barrier of the H2 molecule through the P2C3 film is only 0.18 e V, while the energy barriers of the CO, N2, CO2, and CH4 molecules are 0.77 eV, 0.87 eV, 0.52 eV, and 1.75 eV, respectively. In addition, the P2C3 film has high H2 selectivity toward other gas molecules and high H2 permeability at room temperature. Under 6% tensile strain, 82% hydrogen molecules pass through the film with a H2 permeance of 2.22 × 107 gas permeance unit(GPU), while other molecules cannot across the membrane at all. Therefore, the P2C3 membrane is an excellent material for hydrogen purification.

关键词： two-dimensional membrane hydrogen separation first-principles

Lithium promotes proliferation and suppresses migration of Schwann cells

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Neural Regeneration Research 2020年第10期15卷 1955-1961页

作者： xiao-Kun gu Xin-Rui Li mei-Ling Lu Hui Xu Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education Co-Innovation Center of NeuroregenerationNantong UniversityNantongJiangsu ProvinceChina Department of Hand Surgery Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina State Key Laboratory of Natural Medicines School of Life Science and TechnologyChina Pharmaceutical UniversityNanjingJiangsu ProvinceChina

Schwann cell proliferation,migration and remyelination of regenerating axons contribute to regeneration after peripheral nervous system *** promotes remyelination by Schwann cells and improves peripheral nerve ***,whether lithium modulates other phenotypes of Schwann cells,especially their proliferation and migration remains *** the current study,primary Schwann cells from rat sciatic nerve stumps were cultured and exposed to 0,5,10,15,or 30 mM lithium chloride(LiCl)for 24 *** effects of LiCl on Schwann cell proliferation and migration were examined using the Cell Counting Kit-8,5-ethynyl-2′-deoxyuridine,Transwell and wound healing *** Counting Kit-8 and 5-ethynyl-2′-deoxyuridine assays showed that 5,10,15,and 30 mM LiCl significantly increased the viability and proliferation rate of Schwann ***-based migration assays and wound healing assays showed that 10,15,and 30 mM LiCl suppressed the migratory ability of Schwann ***,the effects of LiCl on the proliferation and migration phenotypes of Schwann cells were mostly *** data indicate that lithium treatment significantly promotes the proliferation and inhibits the migratory ability of Schwann *** conclusion will inform strategies to promote the repair and regeneration of peripheral *** of the animal experiments in this study were ethically approved by the Administration Committee of Experimental Animal Center of Nantong University,China(approval No.20170320-017)on March 2,2017.

关键词： 5-ethynyl-2′-deoxyuridine Cell Counting Kit-8 cell viability lithium migration peripheral nerve proliferation regeneration Schwann cell wound healing assay