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Efficient secretory expression of phospholipase D for the high-yield production of phosphatidylserine and phospholipid derivates from soybean lecithin

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Synthetic and Systems Biotechnology 2023年第2期8卷 273-280页

作者： Peng zhang Jin-Song Gong Zhi-Hao Xie Chang Su xiao-mei zhang Zhi-Ming Rao Zheng-Hong Xu Jin-Song Shi Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of Life Sciences and Health EngineeringJiangnan UniversityWuxi214122PR China National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing School of BiotechnologyJiangnan UniversityWuxi214122PR China Jiangsu Provincial Engineering Research Center for Bioactive Product Processing Jiangnan UniversityWuxi214122PR China Yixing Institute of Food and Biotechnology Co. LtdYixing214200PR China School of Chemical and Material Engineering Jiangnan UniversityWuxi214122PR China

Phospholipase D(PLD)is an essential biocatalyst for the biological production of phosphatidylserine and phospholipid modification.However,the efficient heterologous expression of PLD is limited by its cell toxicity.In this study,a PLD was secretory expressed efficiently in Bacillus subtilis with an activity around 100 U/mL.A secretory expression system containing the signal peptide SPEstA and the dual-promoter PHpaII-SrfA was estab-lished,and the extracellular PLD activity further reached 119.22 U/mL through scale-up fermentation,191.30-fold higher than that of the control.Under optimum reaction conditions,a 61.61%conversion ratio and 21.07 g/L of phosphatidylserine production were achieved.Finally,the synthesis system of PL derivates was established,which could efficiently synthesis novel PL derivates.The results highlight that the secretory expression system constructed in this study provides a promising PLD producing strain in industrial application,and laid the foundation for the biosynthesis of phosphatidylserine and other PL derivates.As far as we know,this work re-ports the highest level of extracellular PLD expression to date and the enzymatic production of several PL der-ivates for the first time.

关键词： Phospholipase D Secretion expression Bacillus subtilis Biosynthesis Enzymatic PLs modification

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Advances in the role of helper T cells in autoimmune diseases

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Chinese Medical Journal 2020年第8期133卷 968-974页

作者： xiao-mei zhang Chun-Yan Liu Zong-Hong Shao Department of Hematology Tianjin Medical University General HospitalTianjin 300052China

Autoimmune diseases are primary immune diseases in which autoreactive antibodies or sensitized lymphocytes destroy and damage tissue and cellular components, resulting in tissue damage and organ dysfunction. Helper T cells may be involved in the pathogenesis of autoimmune diseases under certain conditions. This review summarizes recent research on the role of helper T cells in autoimmune diseases from two aspects, helper T cell-mediated production of autoantibodies by B cells and helper T cell-induced activation of abnormal lymphocytes, and provides ideas for the treatment of autoimmune diseases. The abnormal expression of helper T cells promotes the differentiation of B cells that produce autoantibodies, which leads to the development of different diseases. Among them, abnormal expression of Th2 cells and T follicular helper cells is more likely to cause antibody-mediated autoimmune diseases. In addition, abnormal activation of helper T cells also mediates autoimmune diseases through the production of abnormal cytokines and chemokines. Helper T cells play an essential role in the pathogenesis of autoimmune diseases, and a full understanding of their role in autoimmune diseases is helpful for providing ideas for the treatment of autoimmune diseases.

关键词： Helper T cells B cells Autoimmune disease

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Clonal Transgenerational Effects Transmit for Multiple Generations in a Floating Plant

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1589-1601页

作者： xiao-mei zhang Yu Jin Wei Xue Jun-Qin Gao Ning-Fei Lei Jin-Song Chen Fei-Hai Yu College of Life Science Sichuan Normal UniversityChengdu610101China Institute of Wetland Ecology&Clone Ecology Taizhou UniversityTaizhou318000China School of Ecology and Nature Conservation Beijing Forestry UniversityBeijing100083China College of Ecology and Environment Chengdu University of TechnologyChengdu610059China

Environmental conditions of a parent plant can influence the performance of their clonal offspring,and such clonal transgenerational effects may help offspring adapt to different environments.However,it is still unclear how many vegetative generations clonal transgenerational effects can transmit for and whether it depends on the environmental conditions of the offspring.We grew the ancestor ramets of the floating clonal plant Spirodela polyrhiza under a high and a low nutrient level and obtained the so-called 1^(st)-generation offspring ramets of two types(from these two environments).Then we grew the 1^(st)-generation offspring ramets of each type under the high and the low nutrient level and obtained the so-called 2^(nd)-generation offspring ramets of four types.We repeated this procedure for another five times and analyzed clonal transgenerational effects on growth,morphology and biomass allocation of the 1^(st)-to the 6^(th)-generation offspring ramets.We found positive,negative or neutral(no)transgenerational effects of the ancestor nutrient condition on the offspring of S.polyrhiza,depending on the number of vegetative generations,the nutrient condition of the offspring environment and the traits considered.We observed significant clonal transgenerational effects on the 6^(th)-generation offspring;such effects occurred for all three types of traits(growth,morphology and allocation),but varied depending on the nutrient condition of the offspring environment and the traits considered.Our results suggest that clonal transgenerational effects can transmit for multiple vegetative generations and such impacts can vary depending on the environmental conditions of offspring.

关键词： Clonal plant clonal parental effect maternal effect transgenerational plasticity multi-generation experiment

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The value of epigenetic markers in esophageal cancer

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Frontiers of Medicine 2010年第4期4卷 378-384页

作者： xiao-mei zhang Ming-Zhou GUO Department of Gastroenterology&Hepatology Chinese PLA General HospitalBeijing 100853China

Developing esophageal cancer is a multi-step process that begins with the accumulation of genetic and epigenetic alterations,and leads to the activation of oncogenes and the inactivation or loss of tumor suppressor genes(TSG).In addition to genetic alteration,epigenetic modiﬁcations,and in particular DNA methylation,are recognized as a common molecular alteration in human tumors.In esophageal cancer,aberrant methylation of promoter regions occurs not only in advanced cancer,but also in premalignant lesions.DNA methylation is related to survival time and sensitivity of chemoradiotherapy.This review is mainly focused on epigenetic changes in esophageal cancer and the value of early detection for patient prognosis,treatment choices,and potential target-ing therapy.

关键词： epigenetics DNA methylation esophageal cancer dysplasia

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Analysis of 234 cases of colorectal polyps treated by endoscopic mucosal resection

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World Journal of Clinical Cases 2020年第21期8卷 5180-5187页

作者： Lu Yu Na Li xiao-mei zhang Tao Wang Wei Chen Department of Gastroenterology Maanshan Central HospitalMaanshan 243000Anhui ProvinceChina Department of Gastroenterology Affiliated Hospital of Southeast UniversityNanjing 210009Jiangsu ProvinceChina

BACKGROUND Colorectal polyps refer to all neoplasms that protrude into the intestinal cavity.Researchers believe that 50%-70%of colorectal cancers originate from adenomatous polyps.AIM To investigate the endoscopic morphologic features,pathologic types,and clinical situation;evaluate the efficacy and safety of endoscopic mucosal resection(EMR);and guide clinicians in their daily practice.METHODS Two hundred thirty-four patients who underwent EMR in our hospital from January 1,2018 to December 31,2019 were recruited.Data including sex,age,endoscopic morphology of the polyps,and pathological characteristics were analyzed among groups.RESULTS A total of 295 polyps were resected from the 234 subjects enrolled in the study,of which 4(1.36%)were Yamada type I.There were 75(25.42%)type II,101(34.24%)type III,and 115(38.98%)type IV adenomas.Among them,41 were nonadenomas,110 were low-risk adenomas,139 were high-risk adenomas,and 5 were carcinomas.The differences in distribution were not statistically significant,with P values greater than 0.05.The risk of cancer significantly increased for polyps≥1 cm in diameter(c2=199.825,P=0.00).Regarding the endoscopic morphological features,congestion,erosion,and lobulation were more common on the surface morphology of high-risk adenomas and cancerous polyps(c2=75.257,P=0.00),and most of them were Yamada types III and IV.In all,6 of the 295 polyps could not be removed completely,with a one-time resection rate of 97.97%.There were two cases of postoperative bleeding and no cases of perforation,with an overall complication rate of 0.09%.CONCLUSION Colorectal polyps ranging from non-adenomatous polyps,low-risk adenomas,and high-risk adenomas to adenocarcinomas each has their own endoscopic features,while EMR,as a mature intervention,has good safety and operability and should be promoted clinically,especially at the primary care level.

关键词： Colorectal polyp Endoscopic mucosal resection High-risk adenoma Risk factors Adenocarcinomas 1,4-bis(diphenylphosphino)butane

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Clinical features of postinfectious bronchiolitis obliterans in children undergoing long-term nebulization treatment

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World Journal of Pediatrics 2018年第5期14卷 498-503页

作者： xiao-mei zhang Ai-Zhen Lu Hao-Wei Yang Li-Ling Qian Li-Bo Wang xiao-Bo zhang Respiratory Department Children's Hospital of Fudan University399 Wan Yuan RdShanghaiChina Radiology Department Children's Hospital of Fudan University399 Wan Yuan RdShanghaiChina

Background Limited data are available in relation to the clinical features of PIBO undergoing prolonged nebulization treat-ment with budesonide, terbutaline and ipratropium bromide. This retrospective study aimed to outline the features of clini-cal, high-resolution computed tomography (HRCT) and pulmonary function test (PFT) of PIBO, undergoing maintenance therapy utilizing a triple nebulization treatment and to determine the factors associated with prognosis. Methods Children diagnosed with PIBO were followed up between April 2014 and March 2017. The clinical features after maintenance nebulization treatment for 12 months were thereafter summarized. Results Thirty patients, 21 boys and 9 girls, were enrolled in the study. The median age of patients was 17.4 months, with a range between 3.0 and 33 months. Persistent coughing and wheezing were detected whilst wheezing and crackles were the common manifestations presented. HRCT scans revealed patchy ground and glass opacity, while PFT showed fixed airway obstruction in all patients. Four patients were lost during follow-up. After treatment, the clinical symptoms were improved greatly in all patients (P<0.01). The mean increase in the percentage of TPEF%TE and VPEF%VE were improved greatly (P < 0.01). Images of the HRCT scan indicated marked improvements in 18 patients (81.8%) in comparison with scans obtained pre-treatment. Conclusions Our data suggest a potential role of long-term nebulization treatment of budesonide, terbutaline, ipratropium bromide on PIBO, due to its efficacy as indicated in the improved clinical symptoms, pulmonary functions and CT manifesta-tions identified in the children. New prospective and controlled studies are required to confirm this proposition.

关键词： Clinical features Nebulization treatment Post-infectious bronchiolitis obliterans Prognosis

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Synthesis of phosphorus-containing polyoxamacrocycles with one to two reactive thiophosphoryl chloride groups

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Chinese Chemical Letters 2008年第5期19卷 553-555页

作者： xiao mei zhang Dao Quan Wang Wan Yi Chen Department of Applied Chemistry China Agricultural University Beijing 100094 China College of Chemical Engineering Anhui University of Science and Technology Huainan 232001 China

Novel phosphorus-containing polyoxamacrocycles with one to two reactive thiophosphoryl chloride groups 5, 6, 7, 8 were synthesized from bishydroxyl compounds 1, 2, 3, 4 and thiophosphoryl chloride respectively. Their ... 详细信息

关键词： Phosphorus-containing macrocycle Phosphorus-containing polyoxamacrocycle Thiophosphoryl chloride cyclization

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Synthesis and thermal properties of novel calix[4]arene derivatives containing 1,2,3-triazole moiety via K_2CO_3-catalyzed 1,3-dipolar cycloaddition reaction

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Chinese Chemical Letters 2014年第10期25卷 1346-1348页

作者： Zai-Gang Luo Yu Zhao Feng Xu Chao Ma Xue-mei Xu xiao-mei zhang College of Chemical Engineering Anhui University of Science & Technology

Two calix[4]arene derivatives containing 1,2,3-triazole moiety were synthesized via K2CO3-catalyzed1,3-dipolar cycloaddition reaction between calix[4]arene-based azide and active methylene compounds in good yields.The structures of the two compounds synthesized herein were fully confirmed by 1HNMR,,（13）C NMR,and MS（ESI）.The thermal analysis showed that the mass losses of the synthesized compounds 4 and 5 containing 1,2,3-triazole groups are similar to each other.

关键词： Calixarene derivative 1,2,3-Triazole 1,3-Dipolar cycloaddition Synthesis

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Distinguishing Rectal Cancer from Colon Cancer Based on the Support Vector Machine Method and RNA-sequencing Data

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Current Medical Science 2021年第2期41卷 368-374页

作者： Yan zhang Yuan WU Zi-ying GONG Hai-dan YE xiao kai ZHAO Jie-yi LI xiao-mei zhang Sheng LI Wei ZHU mei WANG Ge-yu LIANG Yun LIU Xin GUAN Dao-yun zhang Bo SHEN Jiangsu Cancer Hospital Jiangsu Institute of Cancer ResearchThe Affiliated Cancer Hospital of Nanjing Medical UniversityNanjing 210009China Shanghai Yunying Medical Technology Co LtdShanghai 201612China Jiaxing Yunying Medical Inspection Co LtdJiaxing 314000China School of Medicine Jiangsu UniversityZhenjiang 212013China School of Public Health Southeast UniversityNanjing 211189China

Colorectal cancer (CRC) is the third most commonly diagnosed cancer worldwide.Several studies have indicated that rectal cancer is significantly different from colon cancer interms of treatment, prognosis, and metastasis. Recently, the differential mRNA expression of coloncancer and rectal cancer has received a great deal of attention. The current study aimed to identifysignificant differences between colon cancer and rectal cancer based on RNA sequencing (RNA-seq)data via support vector machines (SVM). Here, 393 CRC samples from the The Cancer GenomeAtlas (TCGA) database were investigated, including 298 patients with colon cancer and 95 withrectal cancer. Following the random forest (RF) analysis of the mRNA expression data, 96 genessuch as HOXB13, PR4C, and BCLAFI were identified and utilized to build the SVM classificationmodel with the Leave-One-Out Cross-validation (LOOCV) algorithm. In the training (n= 196)and the validation cohorts (n=197), the accuracy (82. 1 % and 82.2 %, respectively) and the AUC(0.87 and 0.91, respectively) indicated that the established optimal SVM classification modeldistinguished colon cancer from rectal cancer reasonably. However, additional experiments arerequired to validate the predicted gene expression levels and functions.

关键词： colon cancer rectal cancer support vector machine classification gene selection

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ABO blood type,diabetes and risk of gastrointestinal cancer in northern China

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World Journal of Gastroenterology 2012年第6期18卷 563-569页

作者： Yan Gong Yun-Sheng Yang xiao-mei zhang Ming Su Jean Wang Jin-Dong Han Ming-Zhou Guo Department of Gastroenterology and Hepatology Chinese PLA General HospitalBeijing 100853China Chinese Academy of Sciences Key Laboratory of Molecular Developmental Biology Center for Molecular Systems BiologyInstitute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China Department of Medicine(Gastroenterology) Johns Hopkins UniversityBaltimoreMD 21287United States

AIM:To explore the potential risk factors related to gastrointestinal cancer in northern China.METHODS:A total of 3314 cases of gastrointestinal cancer(esophageal,gastric,pancreatic and biliary) and 2223 controls(including healthy individuals,glioma and thyroid cancer) were analyzed by case-control study.Multivariable logistic regression analysis was applied to evaluate the association between different cancers and hepatitis B surface antigen,sex,age,blood type,diabetes,or family history of cancer.RESULTS:Type 2 diabetes was significantly associated with gastric,biliary and pancreatic cancer with an OR of 2.0-3.0.Blood type B was significantly associated with esophageal cancer [odd ratio(OR) = 1.53,95% confidence interval(CI) = 1.10-2.14] and biliary cancer(OR = 1.49,95% CI = 1.09-2.05).The prevalence of type 2 diabetes was significantly higher in gastric,biliary and pancreatic cancers compared with other groups,with ORs ranging between 2.0 and 3.0.Family history of cancer was strongly associated with gastrointestinal compared with other cancers.CONCLUSION:Blood type B individuals are susceptible to esophageal and biliary cancer.Type 2 diabetes is significantly associated with gastric,biliary and especially pancreatic cancer.

关键词： 2型糖尿病 ABO血型中国北方风险因素胃癌肠癌 Logistic回归分析消化道肿瘤

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