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Gene Therapy Activates Retinal Pigment Epithelium Cell Proliferation for Age-related Macular Degeneration in a Mouse Model

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Current Medical Science 2023年第2期43卷 384-392页

作者： Yun YUAN Wen KONG xiao-mei liu Guo-hua SHI School of Biomedical Engineering(Suzhou) Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaSuzhou215000China Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of ScienceSuzhou215000China

Objective Age-related macular degeneration(AMD)is a degenerative retinal *** degeneration or death of retinal pigment epithelium(RPE)cells is implicated in the pathogenesis of *** study aimed to activate the proliferation of RPE cells in vivo by using an adeno-associated virus(AAV)vector encodingβ-catenin to treat AMD in a mouse *** Mice were intravitreally injected with AAV2/8-Y733F-VMD2-β-catenin for 2 or 4 weeks,andβ-catenin expression was measured using immunofluorescence staining,real-time quantitative reverse transcription polymerase chain reaction(PCR),and Western *** function ofβ-catenin was determined using retinal flat mounts and laser-induced damage ***,the safety of AAV2/8-Y733F-VMD2-β-catenin was evaluated by multiple intravitreal *** AAV2/8-Y733F-VMD2-β-catenin induced the expression ofβ-catenin in RPE *** activated the proliferation of RPE cells and increased cyclin D1 *** was beneficial to the recovery of laser-induced damage by activating the proliferation of RPE ***,it could induce apoptosis of RPE cells by increasing the expression of Trp53,Bax and caspase3 while decreasing the expression of *** AAV2/8-Y733F-VMD2-β-catenin increasedβ-catenin expression in RPE cells,activated RPE cell proliferation,and helped mice heal from laser-induced eye ***,it could induce the apoptosis of RPE ***,it may be a safe approach for AMD treatment.

关键词： gene therapy adeno-associated virus age-related macular degeneration retinal pigment epithelium cells β-catenin

Controlled Radical Photopolymerization of n-Butyl Methacrylate in Bluk Mediated by HTEMPO

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Chinese Chemical Letters 2006年第8期17卷 1109-1112页

作者： xiao Xuan liu mei HAN Lun Yong DUAN Guang Guo WU Department of Chemistry College of Science Shantou University Shantou 515063

Controlled radical photopolymerization of n-butyl methacrylate（n-BMA） mediated by 4-hydroxy-2,2,6,6-tetramethyl-1-piperidinyloxy（HTEMPO） was carried out in bulk at ambient temperature. It was found that obtained n-BMA homopolymers exhibited narrow polydispersities of around 1.4, which was characterized by GPC. Plots of number-average molecular weight vs. conversion and Ln（[Mo]/[M]） vs. time were liner respectively, indicating that was a controlled photopolymerization.

关键词： photopolymerization nitroxide n-butyl methacrylate HTEMPO.

Herbivore-induced rice resistance against rice blast mediated by salicylic acid

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Insect Science 2020年第1期27卷 49-57页

作者： Kai-Li Zhang Qing-Song liu Hou-Xiang Kang xiao-mei liu Xiu-Ping Chen Yu-Fa Peng Yun-He Li College of Environment and Plant Protection Hainan UniversityHaikouChina State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina College of Life Sciences Xinyang Normal UniversityXinyangHenanChina

In agro-ecosystems,plants are important mediators of interactions between their associated herbivorous insects and microbes,and any change in plants induced by one species may lead to cascading effects on interactions with other ***,such effects are regulated by phytohormones such as jasmonic acid(JA)and salicylic acid(SA).Here,we investigated the tripartite interactions among rice plants,three insect herbivores(Chilo suppressalis,Cnaphalocrocis medinalis or Nilapai-vata lugens),and the causal agent of rice blast disease,the fungus Magnaporthe *** found that pre-infestation of rice by *** or *** but not by *** conferred resistance to *** *** and ***,insect infestation without fungal inoculation induced the accumulation of both JA and SA in rice *** contrast,infestation by *** increased JA levels but reduced SA *** exogenous application of SA but not of JA conferred resistance against *** results suggest that preinfestation by C suppressalis or *** conferred resistance against *** by increasing SA *** findings enhance our understanding of the interactions among rice plant,insects and pathogens,and provide valuable information for developing an ecologically sound strategy for controlling rice blast.

关键词： induced defense jasmonic acid plant-insect-pathogen interaction rice salicylic acid

Glucose Metabolic and Gluconeogenic Pathways Disturbance in the Intrauterine Growth Restricted Adult Male Rats

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Chinese Medical Sciences Journal 2009年第4期24卷 208-212页

作者： xiao-mei liu Jing Kong Wei-wei Song Yan Lu Central Laboratory Department of Surgery Department of Gynaecology and Obstetrics Shengjing Hospital China Medical University Shenyang 110004 China

Objective To explore the molecular mechanism of type 2 diabetes in intrauterine growth restricted adult rats through determination of blood glucose and expression of gluconeogenic enzymes in *** Male intrauterine growth restriction(IUGR) offspring induced by maternal protein-malnutrition and normal controls were *** body weights of offspring rats were weighted from birth to 12 weeks of *** plasma glucose and insulin levels were determined by glucose oxidase method and enzyme-linked immunosorbent assay(ELISA) respectively at 1 week,8 weeks,and 12 *** proliferator-activated receptor-γ coactivator-1α(PGC-1α),phosphoenolpyruvate carboxykinase(PEPCK),and glucose-6-phosphatase(G6Pase) mRNA and protein levels in liver were measured by real time RT-PCR and Western blot in newborn rats(Week 1) and adult rats(Week 12).Results Birth weights of IUGR rats were significantly lower than those of controls until 4 weeks later,when IUGR rats caught up to *** 8 and 12 weeks,the growth of IUGR rats surpassed that of *** significant differences were observed in blood glucose and insulin levels at newborn rats between the two ***,by the end of 8 weeks IUGR rats developed hyperinsulinemia and high insulin resistance *** the age of 12 weeks,IUGR rats had mild fasting *** addition,hepatic PGC-1α mRNA and protein levels as well as hepatic mRNA levels of PEPCK and G6Pase at Week 1 and Week 12 in IUGR rats were all significantly higher than those of controls(P<0.05).Conclusions As a result of intrauterine malnutrition,the expression of gluconeogenic genes is exaggerated in *** change stays through adulthood and may contribute to the pathogenesis of type 2 diabetes.

关键词： intrauterine growth restriction insulin resistance gluconeogenesis

Blocking postsynaptic density-93 binding to C-X3-C motif chemokine ligand 1 promotes microglial phenotypic transformation during acute ischemic stroke

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Neural Regeneration Research 2023年第5期18卷 1033-1039页

作者： xiao-Wei Cao Hui Yang xiao-mei liu Shi-Ying Lou Li-Ping Kong Liang-Qun Rong Jun-Jun Shan Yun Xu Qing-Xiu Zhang Department of Neurology of Drum Tower Hospital Medical School and the State Key Laboratory of Pharmaceutical BiotechnologyNanjing UniversityNanjingJiangsu ProvinceChina Nanjing Drum Tower Clinical College of Xuzhou Medical University NanjingJiangsu ProvinceChina Institute of Brain Sciences Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Key Laboratory for Molecular Medicine Medical School of Nanjing UniversityNanjingJiangsu ProvinceChina Jiangsu Province Stroke Center for Diagnosis and Therapy NanjingJiangsu ProvinceChina Nanjing Neurology Clinic Medical Center NanjingJiangsu ProvinceChina Department of Neurology Lianyungang Municipal HospitalAffiliated Hospital of Xuzhou Medical UniversityLianyungangJiangsu ProvinceChina Department of Neurosurgery of Drum Tower Hospital Medical School and the State Key Laboratory of Pharmaceutical BiotechnologyNanjing UniversityNanjingJiangsu ProvinceChina Department of Neurosurgery Affiliated Xuzhou Municipal Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Jiangsu Key Laboratory of Immunity and Metabolism Department of Pathogen Biology and Immunology and Laboratory of Infection and ImmunityXuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Neurology Second Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina

We previously reported that postsynaptic density-93 mediates neuron-microglia crosstalk by interacting with amino acids 357–395 of C-X3-C motif chemokine ligand 1(CX3 CL1) to induce microglia polarization. More importantly, the peptide Tat-CX3 CL1(comprising amino acids 357–395 of CX3 CL1) disrupts the interaction between postsynaptic density-93 and CX3 CL1, reducing neurological impairment and exerting a protective effect in the context of acute ischemic stroke. However, the mechanism underlying these effects remains unclear. In the current study, we found that the pro-inflammatory M1 phenotype increased and the anti-inflammatory M2 phenotype decreased at different time points. The M1 phenotype increased at 6 hours after stroke and peaked at 24 hours after perfusion, whereas the M2 phenotype decreased at 6 and 24 hours following reperfusion. We found that the peptide Tat-CX3 CL1(357–395 aa) facilitates microglial polarization from M1 to M2 by reducing the production of soluble CX3 CL1. Furthermore, the a disintegrin and metalloprotease domain 17(ADAM17) inhibitor GW280264 x, which inhibits metalloprotease activity and prevents CX3 CL1 from being sheared into its soluble form, facilitated microglial polarization from M1 to M2 by inhibiting soluble CX3 CL1 formation. Additionally, Tat-CX3 CL1(357–395 aa) attenuated long-term cognitive deficits and improved white matter integrity as determined by the Morris water maze test at 31–34 days following surgery and immunofluorescence staining at 35 days after stroke, respectively. In conclusion, Tat-CX3 CL1(357–395 aa) facilitates functional recovery after ischemic stroke by promoting microglial polarization from M1 to M2. Therefore, the Tat-CX3 CL1(357–395 aa) is a potential therapeutic agent for ischemic stroke.

关键词： a disintegrin and metalloprotease domain 17 cerebral ischemia/reperfusion C-X3-C motif chemokine ligand 1 GW280264x microglia neuroinflammation postsynaptic density-93 Tat-CX3CL1(357–395aa)

Human hair follicle-derived mesenchymal stem cells:Isolation,expansion,and differentiation

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World Journal of Stem Cells 2020年第6期12卷 462-470页

作者： Bo Wang xiao-mei liu Zi-Nan liu Yuan Wang Xing Han Ao-Bo Lian Ying Mu Ming-Hua Jin Jin-Yu liu Department of Toxicology School of Public HealthJilin UniversityChangchun 130021Jilin ProvinceChina Research Center for Analytical Instrumentation Institute of Cyber-Systems and ControlState Key Laboratory of Industrial Control TechnologyZhejiang UniversityHangzhou 310000Zhejiang ProvinceChina

Hair follicles are easily accessible skin appendages that protect against cold and potential *** follicles contain various pools of stem cells,such as epithelial,melanocyte,and mesenchymal stem cells(MSCs)that continuously self-renew,differentiate,regulate hair growth,and maintain skin ***,MSCs derived from the dermal papilla or dermal sheath of the human hair follicle have received attention because of their accessibility and broad differentiation *** this review,we describe the applications of human hair follicle-derived MSCs(hHF-MSCs)in tissue engineering and regenerative *** have described protocols for isolating hHF-MSCs from human hair follicles and their culture condition in *** also summarize strategies for maintaining hHF-MSCs in a highly proliferative but undifferentiated state after repeated in vitro passages,including supplementation of growth factors,3D suspension culture technology,and 3D aggregates of *** addition,we report the potential of hHF-MSCs in obtaining induced smooth muscle cells and tissue-engineered blood vessels,regenerated hair follicles,induced red blood cells,and induced pluripotent stem *** summary,the abundance,convenient accessibility,and broad differentiation potential make hHF-MSCs an ideal seed cell source of regenerative medical and cell therapy.

关键词： Human hair follicle Regenerative therapy Mesenchymal stem cell Tissue engineering Cell differentiation

Employing a random forest model to forecast the likelihood of coronary artery lesions in Kawasaki disease: a study centered on four biomarkers

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Medical Data Mining 2024年第3期7卷 1-7页

作者： Chu-Xiong Gong Yue-Wen Li Ya-Min Li Zi-Yu Wang Hui-Qing Gao xiao-mei liu Department of Infectious Diseases Children’s Hospital Affiliated of Kunming Medical UniversityKunming Children’s HospitalKunming 650228China Department of Rehabilitation and Palliative Medicine The Third Affiliated Hospital of Kunming Medical UniversityKunming 650000China

Background:Kawasaki disease is an acute immune vasculitis,which is more common in children under 5 years *** disease mainly affects the cardiovascular system,especially the coronary *** coronary artery damage occurs,it can significantly impact the patient’s ***,in some countries and regions,Kawasaki disease has become a common acquired heart ***:First,univariate analysis was conducted on each predictive ***,Least Absolute Shrinkage and Selection Operator and random forest algorithms were used to screen all predictive factors,and the prediction model was evaluated using receiver operating characteristic curve,calibration curve,and Decision Curve ***:This study,based on data from 228 Kawasaki disease patients,utilized a random forest model to identify four predictive factors:white blood cell count,creatine kinase isoenzyme MB,albumin,and neutrophil *** factors were used to construct a prediction model,which achieved an area under the curve of ***:We developed a forest plot based on white blood cell count,creatine kinase isoenzyme MB,albumin,and neutrophil count to effectively predict the occurrence of coronary artery lesions in Kawasaki disease.

关键词： Kawasaki disease coronary artery lesions nomogram machine learning

A frozen-thawed embryo transfer program improves the embryo utilization rate

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Chinese Medical Journal 2009年第17期122卷 1974-1978页

作者： ZHOU Feng LIN xiao-na TONG xiao-mei LI Chao liu liu JIN xiao-ying ZHU Hai-yan ZHANG Song-ying Assisted Reproduction Unit Department of Obstetrics andGynecology Sir Run Run Shaw Hospital Zhejiang UniversitySchool of Medicine Hangzhou Zhejiang 310016 China

Background Frozen-thawed embryo transfer （FET） is the most common way to prevent serious late ovarian hyperstimulation syndrome and increase the cumulative pregnancy rate. We evaluated the effectiveness of an FET program for improving the embryo implantation and clinical pregnancy rates, and ultimate embryo utilization rate in infertility treatment. Methods Patients undergoing in vitro fertilisation （IVF） cycles from January 2006 to June 2008 were enrolled, including 179 patients who had undergone the first FET cycle after controlled ovarian hyperstimulation （COH） in which all embryos were frozen （group C1） and 1306 patients who had COH with fresh embryo transfer （ET）（group T1）. Logistic regression was used to model the embryo implantation and clinical pregnancy rates based on the mother＇s age, numbers of oocytes retrieved, embryos transferred and high-quality embryos transferred. The embryo implantation and clinical pregnancy rates were also compared between two groups after adjusting for age, the numbers of oocytes retrieved and the numbers of embryos transferred. Results Logistic regression analysis confirmed that embryo implantation and clinical pregnancy rates in group C1 were both significantly higher than those in group T1 after adjusting for confounding factors （43.6% vs 29.0%, 63.1% vs 47.0%, respectively; P 〈0.01）. The embryo implantation and clinical pregnancy rates were consistently higher in group C1 by comparing the age groups ≥35 or 〈35 years. The clinical pregnancy rates for the numbers of oocytes retrieved per cycle being ≥15 or 〈15 were higher in group C1, as was the embryo implantation rate. These differences were statistically significant for oocyte numbers 〉15 （P 〈0.05）. The embryo implantation and clinical pregnancy rates in group C1 were both significantly higher than in group T1 when two or three embryos were transferred （P 〈0.05）. Conclusion A program of freezing all embryos and performing FET improved the rates of embryo implantation and c

关键词： embryo frozen-thawed embryo transfer pregnancy rate endometrial receptivity

Application of LC-MS based glutathione-trapped reactive metabolites in the discovery of toxicity of traditional Chinese medicine

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TMR Modern Herbal Medicine 2018年第4期1卷 209-219页

作者： xiao-mei liu Hong Lv xiao-Ming Wang Ya-Qing Guo Ting-Ting Li Gui-Xiang Pan Tianjin State Key Laboratory of Modern Chinese Medicine Tianjin University of Traditional Chinese Medicine Tianjin China. Second Affiliated hospital of Tianjin University of Traditional Chinese Medicine Tianjin China.

We summarized the discovery of liquid chromatography mass spectrometry （LC-MS） based glutathione （GSH） capture of reactive metabolites in traditional Chinese medicine （TCM）, which provides scientific basis for further research and clinical application of Chinese medicine toxicity discovery. This dissertation overviews relevant literatures of GSH capture of reactive metabolites in recent years. And then we review the principles and methods of LC-MS based GSH capture of reactive metabolites, as well as the research progress in the discovery of toxicity of TCM including pyrrolizidine alkaloids, furans and quinoid species. The review shows that the representative compounds of TCM includes adonifoline, lasiocarpine, diosbulbin B and safrol are well dctected by LC-MS based GSH capture technique. And the main analytic systems of LC-MS are triple quadrupole and Q-Trap mass spectrometer. Constant neutral loss scan （CNLS）, precursor ion scan （PIS） and multiple reaction monitor （MRM） are main detection methods to monitor the characteristic GSH conjugate fragmentations. The approach of LC-MS based GSH-trapped metabolites has a good application prospect in the discovery of toxic components of TCM.

关键词： glutathione conjugation Liquid Chromatography Mass Spectrometry Reactive metabolites Toxicity traditional Chinese medicine